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Molecular Breeding for Abiotic Stress Tolerance in Crops (Second Edition)

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: closed (28 June 2025) | Viewed by 1561

Special Issue Editor


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Guest Editor
Department of Agricultural and Forest Sciences, Tuscia University, Via S. C. de Lellis, snc, 01100 Viterbo, Italy
Interests: plant population genetics; plant evolution and domestication; in situ and ex situ conservation of plant germplasm; molecular characterization; molecular markers; molecular evolution; plant breeding
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Special Issue Information

Dear Colleagues,

There is a crucial need for plant varieties which are resistant/tolerant to abiotic stresses in the face of the consequences of climate change, such as heat waves, drought events, and the rise in saline-cultivated land. Nowadays, molecular tools are very useful for better understanding plant mechanisms in reacting to stresses and for accelerating the process of selecting the best germplasm throughout the different steps of a breeding program, from germplasm characterization to variety registration. This Special Issue titled “Molecular Breeding for Abiotic Stress Tolerance in Crops (Second Edition)” aims to bring together the latest research which uses new molecular approaches to improve crop varieties.

This Special Issue attempts to cover all aspects relating to studies of plant responses to abiotic stresses including physiology, biochemistry, cell biology, and molecular biology, in addition to literature reviews of subjects related to this field. Potential papers may focus on the experimental, theoretical, and computational methodologies used in molecular breeding across different plant species, including crops and their wild relatives.

Potential topics include the following:

  • Plant response to abiotic stresses;
  • Plant breeding;
  • Physiology;
  • Biochemistry;
  • Cell biology;
  • Molecular biology.

Dr. Mario A. Pagnotta
Guest Editor

Manuscript Submission Information

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Keywords

  • plant breeding
  • advance molecular approach
  • molecular characterization
  • environmental stresses plant tolerance
  • resistant genes
  • QTLs
  • drought
  • salinity
  • climatic changes

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Published Papers (2 papers)

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Research

19 pages, 4633 KiB  
Article
The Nuclear Transcription Factor SlNF-YC9 Regulates the Protrusion of Tomato Fruit Tip
by Zihan Gao, Ting Long, Pengyu Guo, Junjie Luo, Xiaoqian Nie, Qiaoli Xie, Guoping Chen and Zongli Hu
Int. J. Mol. Sci. 2025, 26(13), 6511; https://doi.org/10.3390/ijms26136511 - 6 Jul 2025
Viewed by 18
Abstract
NF-Y transcriptional regulators play crucial roles in diverse biological processes in plants, primarily through the formation of NF-Y complexes that bind to specific DNA motifs. These complexes modulate the expression of downstream genes, which influence plant development and growth. In our research, the [...] Read more.
NF-Y transcriptional regulators play crucial roles in diverse biological processes in plants, primarily through the formation of NF-Y complexes that bind to specific DNA motifs. These complexes modulate the expression of downstream genes, which influence plant development and growth. In our research, the function of the NF-Y family C subunit member SlNF-YC9 gene in tomato was investigated with the CRISPR/Cas9 method. In contrast to the WT (wild type), the mutant CR-SlNF-YC9 exhibited a prominent protrusion at the fruit tip. The quantitative PCR analysis displayed that the transcription levels of genes associated with auxin transport (PIN4, PIN5, and PIN9) as well as auxin response genes (ARF7 and LAX3) were enhanced in the CR-SlNF-YC9 fruits than in the WT. Analysis of dual-luciferase reporter and EMSA assays showed that the SlNF-YC9-YB13b-YA7a trimer specifically binds the FUL2 promoter and represses its expression. In conclusion, our results suggest that SlNF-YC9 is crucial in influencing tomato fruit shape by the formation of NF-Y heterotrimeric complexes. Full article
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20 pages, 4483 KiB  
Article
SlUPA-like, a bHLH Transcription Factor in Tomato (Solanum lycopersicum), Serves as the Crosstalk of GA, JA and BR
by Pengyu Guo, Xin Cheng, Yunshu Wang, Guoping Chen, Xuqing Chen, Yingwu Yang, Xiuhai Zhang and Zongli Hu
Int. J. Mol. Sci. 2024, 25(24), 13419; https://doi.org/10.3390/ijms252413419 - 14 Dec 2024
Cited by 1 | Viewed by 1105
Abstract
The bHLH (basic Helix–Loop–Helix) transcription factor serves as pivotal controller in plant growth and development. In a previous study, the overexpression of SlUPA-like in Solanum lycopersicum L. Ailsa Craig (AC++) altered the JA (Jasmonic acid) response and endogenous GA (Gibberellic acid) [...] Read more.
The bHLH (basic Helix–Loop–Helix) transcription factor serves as pivotal controller in plant growth and development. In a previous study, the overexpression of SlUPA-like in Solanum lycopersicum L. Ailsa Craig (AC++) altered the JA (Jasmonic acid) response and endogenous GA (Gibberellic acid) content. However, the detailed regulation mechanism was not fully explored. In the present research, we found that the overexpression of SlUPA-like influenced the accumulation of GA, JA and BR (Brassinolide). RNA-Seq data illustrated that the expression levels of genes related to these plant hormones were significantly affected. Additionally, the interaction of SlUPA-like with SlMYB21, SlMYC2 and SlDELLA was characterized by employing Y2H (Yeast Two-Hybrid) and BiFC (Bimolecular Fluorescence Complementation) assay. Furthermore, Dual-LUC (Dual-Luciferase) assay and EMSA (Electrophoretic Mobility Shift Assay) identified that SlUPA-like directly targeted the E-box motif in the promoter of SlGID2 and activated the transcription of SlGID2. These results shed light on the potential role of SlUPA-like in mediating crosstalk among multiple plant hormones and established a robust theoretical framework for further unraveling the functions of SlUPA-like transcription factors in the context of plant growth and hormone signal transduction. Full article
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