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Molecular Mechanism of Extracellular Vesicles in Human Diseases

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 20 September 2025 | Viewed by 1536

Special Issue Editor


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Guest Editor
1. Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
2. Buda Hospital of the Hospitaller Order of Saint John of God, Budapest, Hungary
Interests: extracellular vesicles; protein corona; surface interactions; immunology; rheumatology

Special Issue Information

Dear Colleagues,

Extracellular vesicles (EVs) are nano-sized, membrane-bound particles released by all cell types, playing essential roles in intercellular communication. They facilitate the transfer of proteins, lipids, and nucleic acids, impacting various physiological processes and disease mechanisms. EVs are gaining recognition for their potential in diagnostics and therapeutics, serving as biomarkers for diseases such as cancer, infections, and immunological and neurodegenerative disorders. Their ability to transport cargo across biological barriers positions them as promising vehicles for drug delivery and regenerative medicine.
This Special Issue aims to focus on the molecular mechanisms through which EVs contribute to the pathophysiology of human diseases. As a journal dedicated to advancing molecular science, this Special Issue emphasizes studies that explore the intricate biomolecular interactions of EVs in both clinical and model systems. While purely clinical studies fall outside the scope of this Issue, we welcome submissions that incorporate biomolecular experiments alongside clinical insights or studies utilizing relevant models. Your contributions can significantly enhance our understanding of the role of EVs in disease processes and potential therapeutic applications.

Dr. Eszter Ágnes Tóth
Guest Editor

Manuscript Submission Information

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Keywords

  • extracellular vesicles
  • intercellular communication
  • disease pathophysiology
  • biomarkers
  • nanoscience
  • biomolecular interactions

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Published Papers (1 paper)

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19 pages, 3764 KiB  
Article
Isolation and Characterization of Tissue-Derived Extracellular Vesicles from Mouse Lymph Nodes
by Bernadett R. Bodnár, Sayam Ghosal, Brachyahu M. Kestecher, Panna Királyhidi, András Försönits, Nóra Fekete, Edina Bugyik, Zsolt I. Komlósi, Éva Pállinger, György Nagy, Edit I. Buzás and Xabier Osteikoetxea
Int. J. Mol. Sci. 2025, 26(13), 6092; https://doi.org/10.3390/ijms26136092 - 25 Jun 2025
Viewed by 1212
Abstract
Extracellular vesicles (EVs) are lipid membrane-enclosed particles released by all cells and can be isolated from various sources, even from solid tissues. This study focuses on isolating and characterizing EVs from mouse lymph nodes (LNs). Male C57BL/6 mice were injected with complete Freund’s [...] Read more.
Extracellular vesicles (EVs) are lipid membrane-enclosed particles released by all cells and can be isolated from various sources, even from solid tissues. This study focuses on isolating and characterizing EVs from mouse lymph nodes (LNs). Male C57BL/6 mice were injected with complete Freund’s adjuvant, with or without ovalbumin. Inguinal and popliteal LNs were incised 9 days after immunization, and EV isolation was carried out using a combination of differential centrifugation and size-exclusion chromatography. The characteristic morphology of small and large EVs was confirmed by transmission electron microscopy. Particle size distribution and concentration were determined by nanoparticle tracking analysis, while protein and lipid contents were measured by bicinchoninic acid assay, and sulfo-phospho-vanillin assays, respectively, to calculate the protein-to-lipid ratio. Immune and EV markers were analyzed by using flow cytometry and Western blot assay, revealing significant changes between immunized mice compared to controls. This study establishes a novel protocol for isolating and characterizing EVs from LNs and highlights the impact of immunization on EV properties, offering insights into their roles in immune processes. Full article
(This article belongs to the Special Issue Molecular Mechanism of Extracellular Vesicles in Human Diseases)
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