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Molecular Research on Parasitic Infection

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 20 February 2026 | Viewed by 451

Special Issue Editors


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Guest Editor
Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-786 Warsaw, Poland
Interests: parasitic infection

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Guest Editor
Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-786 Warsaw, Poland
Interests: immunology; microbiology; veterinary; biochemistry
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Special Issue Information

Dear Colleagues,

Parasitic infection remains a major global health challenge for both human and veterinary medicine, affecting millions of people and causing a significant socioeconomic burden, particularly in low- and middle-income countries. Advances in molecular biology have revolutionized our understanding of parasite biology, host–parasite interactions, and immune mechanism evasion. These insights are crucial not only for improving diagnostic tools, but also for designing effective therapies and control strategies. Despite these advances, many parasitic diseases remain understudied, and emerging parasitic diseases pose new challenges. This Special Issue provides a platform to highlight the latest molecular discoveries and their translational applications in parasitology.

We invite researchers to contribute original research articles, reviews, and case studies on the molecular and cellular mechanisms underlying parasitic infections. Topics of interest include, but are not limited to, parasite genomics, transcriptomics, proteomics, host–parasite interactions, mechanisms of drug resistance, and novel therapeutic approaches. By bringing together cutting-edge research, this Special Issue aims to foster interdisciplinary collaboration and accelerate the development of innovative strategies to mitigate the effects of parasitic infections.

Prof. Dr. Marcin Wiśniewski
Prof. Dr. Piotr Bąska
Guest Editors

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Keywords

  • parasitic infections
  • host–parasite interactions
  • parasite genomics
  • drug-resistance mechanisms
  • transcriptomics
  • proteomics
  • diagnostic tools
  • immune evasion
  • therapeutic strategies

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Published Papers (1 paper)

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Research

25 pages, 7475 KiB  
Article
Human Dialyzable Leukocyte Extract Enhances Albendazole Efficacy and Promotes Th1/Th2-Biased Lymphocyte and Antibody Responses in Peritoneal Cavity of Murine Model of Mesocestoides vogae Infection
by Gabriela Hrčková, Dagmar Mudroňová, Katarína Reiterová, Serena Cavallero and Ilaria Bellini
Int. J. Mol. Sci. 2025, 26(14), 6994; https://doi.org/10.3390/ijms26146994 - 21 Jul 2025
Viewed by 160
Abstract
Human leukocyte extract (HLE), a non-immunogenic dialyzable leukocyte preparation (<10 kDa), may serve as a safe adjuvant in immunotherapy. We investigated the effects of albendazole (ABZ), HLE, and their combination in Mesocestoides vogae infected mice, focusing on lymphoid cells in the peritoneal cavity, [...] Read more.
Human leukocyte extract (HLE), a non-immunogenic dialyzable leukocyte preparation (<10 kDa), may serve as a safe adjuvant in immunotherapy. We investigated the effects of albendazole (ABZ), HLE, and their combination in Mesocestoides vogae infected mice, focusing on lymphoid cells in the peritoneal cavity, the site of larval proliferation and parasite-induced immunosuppression. Peritoneal lymphoid cells were analysed by flow cytometry and qPCR. Cells proliferative responses to ConA, LPS, and parasite excretory/secretory (E/S) antigens, cytokine production (ELISA), IgM and IgG isotypes in exudates and parasite antigen recognition (Western blot) were assessed. Efficacy was measured by larval burden and 14-3-3 gene expression in larvae. HLE combined with ABZ enhanced larval clearance and suppressed 14-3-3 gene expression in larvae. HLE and combination therapy increased CD3+ T cell frequencies, especially CD3+high, reduced regulatory CD3+/IL-10 Tregs and expression of Foxp3+. All treatments diminished CD19+/IL-10+ Bregs, correlating with lower CD9 and Atf3 mRNA levels compared to infected mice. Transcription factors T-bet expression was strongly upregulated, while GATA3 was moderately elevated. IFN-γ production and T/B cell proliferation were restored after HLE and combination therapy, partially, even in the presence of E/S antigens. IgM and total IgG levels against parasite antigens declined, while Th1-associated IgG2a increased in ABZ+HLE and HLE-treated groups. Albendazole failed to reverse the immunosuppressive Treg-type immunity but was more effective in reducing Breg populations and their functions. HLE enhanced ABZ efficacy by restoring Th1 responsiveness, reducing Treg/Breg activity, and modulating antibody profiles. It represents a promising immunomodulatory adjuvant in the treatment of the infections associated with Th2/Treg-driven immunosuppression. Full article
(This article belongs to the Special Issue Molecular Research on Parasitic Infection)
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