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Molecular and Genomic Aspects of Viral Pathogens

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 31 October 2025 | Viewed by 101

Special Issue Editor


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Guest Editor
Department of Virology and Viral Diseases of Animals, National Veterinary Research Institute, Partyzantów 57 Avenue, 24-100 Puławy, Poland
Interests: whole genome sequencing; molecular biology; parasite; cat

Special Issue Information

Dear Colleagues,

In recent decades, rapid advancements in the field of virology, driven by breakthroughs in molecular biology and genomic technologies, have enhanced our understanding of viral pathogens. The proposed Special Issue, “Molecular and Genomic Aspects of Viral Pathogens”, aims to provide a comprehensive overview of current research on the genetic and molecular mechanisms that underpin viral infection, replication, evolution, and host interaction. This issue will cover a wide range of topics, including, but not limited to, viral genome organization and variability, host–pathogen molecular interactions, the mechanisms of viral replication and gene expression, antiviral resistance, and the applications of high-throughput sequencing in virus discovery and surveillance. Special attention will also be paid to emerging and re-emerging viral pathogens, particularly those with the potential to cause a pandemic. By gathering original research articles, reviews, and short communications from international experts, this Special Issue seeks to foster interdisciplinary dialogue and present novel insights into viral biology, with the ultimate goal of supporting the development of innovative diagnostic tools, therapeutic strategies, and public health responses.

Dr. Joanna Dąbrowska
Guest Editor

Manuscript Submission Information

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Keywords

  • viral pathogens
  • genetic and molecular mechanisms
  • viral infection
  • viral replication
  • host interaction

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Published Papers (1 paper)

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Research

20 pages, 1784 KiB  
Article
Detection of Feline Coronavirus Membrane Gene Based on Conventional Revere Transcription-Polymerase Chain Reaction, Nested Reverse Transcription-Polymerase Chain Reaction, and Reverse Transcription-Quantitative Polymerase Chain Reaction: A Comparative Study
by Chiraphat Kopduang, Witsanu Rapichai, Chalandhorn Leangcharoenpong, Piyamat Khamsingnok, Thanapol Puangmalee, Siriluk Ratanabunyong, Amonpun Rattanasrisomporn, Thanawat Khaoiam, Hieu Van Dong, Kiattawee Choowongkomol and Jatuporn Rattanasrisomporn
Int. J. Mol. Sci. 2025, 26(14), 6861; https://doi.org/10.3390/ijms26146861 - 17 Jul 2025
Abstract
Feline coronavirus (FCoV) is a major pathogen causing feline infectious peritonitis (FIP), a lethal disease in cats, necessitating accurate diagnostic methods. This study developed and compared novel primers targeting the FCoV membrane (M) gene for enhanced detection. Specific primers were designed [...] Read more.
Feline coronavirus (FCoV) is a major pathogen causing feline infectious peritonitis (FIP), a lethal disease in cats, necessitating accurate diagnostic methods. This study developed and compared novel primers targeting the FCoV membrane (M) gene for enhanced detection. Specific primers were designed for the M gene and their performance evaluated using reverse transcription-PCR (RT-PCR), nested RT-PCR, and reverse transcription-quantitative PCR (RT-qPCR) on 80 clinical effusion samples from cats suspected of FIP. Specificity of assays was tested against other feline viruses, with sensitivity being assessed via serial dilutions of FCoV RNA. RT-qPCR had the highest sensitivity, detecting 9.14 × 101 copies/µL, identifying 93.75% of positive samples, followed by nested RT-PCR (87.50%, 9.14 × 104 copies/µL) and RT-PCR (61.25%, 9.14 × 106 copies/µL). All assays had 100% specificity, with no cross-reactivity to other viruses. The nested RT-PCR and RT-qPCR outperformed RT-PCR significantly, with comparable diagnostic accuracy. The novel primers targeting the FCoV M gene, coupled with RT-qPCR, delivered unparalleled sensitivity and robust reliability for detecting FCoV in clinical settings. Nested RT-PCR was equally precise and amplified diagnostic confidence with its high performance. These cutting-edge assays should revolutionize FCoV detection, offering trusted tools that seamlessly integrate into veterinary practice, empowering clinicians to manage feline infectious peritonitis with unprecedented accuracy and speed. Full article
(This article belongs to the Special Issue Molecular and Genomic Aspects of Viral Pathogens)
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