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Special Issue "Recent Advances in Bioseparation Technologies for the Capture of Emerging Biomacromolecules"

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Macromolecules".

Deadline for manuscript submissions: 31 July 2023 | Viewed by 932

Special Issue Editors

1. Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2819-516 Caparica, Portugal
2. UCIBIO – Applied Molecular Biosciences Unit, Chemistry Department, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal
3. CICS-UBI - Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, Portugal
Interests: method development and validation; electrochemical detection; proteomics; protein biomarkers
Special Issues, Collections and Topics in MDPI journals
CICS-UBI – Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, Portugal
Interests: separation and purification methodologies; recombinant proteins; therapeutic nucleic acids
Special Issues, Collections and Topics in MDPI journals
1. Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior (CICS-UBI), 6201-506 Covilhã, Portugal
2. Laboratório de Farmaco-Toxicologia, UBIMedical, Universidade da Beira Interior (CICS-UBI), 6201-506 Covilhã, Portugal
Interests: toxicology; analytical method development; recreational drugs; natural psychoactive substances; therapeutic drug monitoring; sample preparation; alternative samples; miniaturized extraction procedures
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Several research groups are devoted to the dissemination of new and original knowledge in all fields of bioseparation sciences. In order to assemble these findings, a Special Issue entitled “Recent Advances in Bioseparation Technologies for the Capture of Emerging Biomacromolecules” is being launched. This Special Issue aims to gather scientific full papers, short communications, and review articles covering novel separation and isolation methods based on chromatographic and nonchromatographic platforms, demonstrating a huge societal impact—for instance, advances in the purification of biomacromolecules or pathological factors using microdevices and advanced aqueous two-phase systems. Developments with interdisciplinary approaches are particularly welcome, and systems should be proven with suitable biological, therapeutic, or biotechnological challenging samples.

Presenting a very broad scope, this Special Issue welcomes but is not limited to the following technologies and emergent applications: molecular diagnostics, biosensors and bioengineering, drug development and pharmaceutical analysis, microfluidics and nanotechnology, omics analysis (such as proteomics, metabolomics or glycomics), food science, neuroscience, biochemical and forensic analysis, industrial processes, and method development.

This issue will serve as a collection of papers for academia or as a reference tool for researchers, including health and industry professionals, particularly those working in the fields of biotechnology, biomedicine, biology, chemistry, drug design, medicine, and pharmaceutical sciences.

We look forward to receiving your contributions.

Dr. Luís Passarinha
Dr. Ângela Sousa
Prof. Dr. Eugenia Gallardo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nucleic acids
  • proteins
  • purification
  • isolation

Published Papers (1 paper)

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Research

Article
Specific Six-Transmembrane Epithelial Antigen of the Prostate 1 Capture with Gellan Gum Microspheres: Design, Optimization and Integration
Int. J. Mol. Sci. 2023, 24(3), 1949; https://doi.org/10.3390/ijms24031949 - 18 Jan 2023
Viewed by 730
Abstract
This work demonstrates the potential of calcium- and nickel-crosslinked Gellan Gum (GG) microspheres to capture the Six-Transmembrane Epithelial Antigen of the Prostate 1 (STEAP1) directly from complex Komagataella pastoris mini-bioreactor lysates in a batch method. Calcium-crosslinked microspheres were applied in an ionic exchange [...] Read more.
This work demonstrates the potential of calcium- and nickel-crosslinked Gellan Gum (GG) microspheres to capture the Six-Transmembrane Epithelial Antigen of the Prostate 1 (STEAP1) directly from complex Komagataella pastoris mini-bioreactor lysates in a batch method. Calcium-crosslinked microspheres were applied in an ionic exchange strategy, by manipulation of pH and ionic strength, whereas nickel-crosslinked microspheres were applied in an affinity strategy, mirroring a standard immobilized metal affinity chromatography. Both formulations presented small diameters, with appreciable crosslinker content, but calcium-crosslinked microspheres were far smoother. The most promising results were obtained for the ionic strategy, wherein calcium-crosslinked GG microspheres were able to completely bind 0.1% (v/v) DM solubilized STEAP1 in lysate samples (~7 mg/mL). The target protein was eluted in a complexed state at pH 11 with 500 mM NaCl in 10 mM Tris buffer, in a single step with minimal losses. Coupling the batch clarified sample with a co-immunoprecipitation polishing step yields a sample of monomeric STEAP1 with a high degree of purity. For the first time, we demonstrate the potential of a gellan batch method to function as a clarification and primary capture method towards STEAP1, a membrane protein, simplifying and reducing the costs of standard purification workflows. Full article
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