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Fishes
Special Issues
Advances in Germplasm Resources and Genetic Breeding of Aquatic Animals
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Advances in Germplasm Resources and Genetic Breeding of Aquatic Animals

A special issue of Fishes (ISSN 2410-3888). This special issue belongs to the section "Genetics and Biotechnology".

Deadline for manuscript submissions: 10 June 2025 | Viewed by 2387

Special Issue Editors

Dr. Li-Min Wu

E-Mail Website
Guest Editor
College of Fisheries, Henan Normal University, Xinxiang, China
Interests: sex determination and differentiation; reproduction; development; oogenesis; gynogenesis
Special Issues, Collections and Topics in MDPI journals
Dr. Dong-Neng Jiang

E-Mail Website
Guest Editor
College of Fisheries, Guangdong Ocean University, Zhanjiang, China
Interests: reproduction; development; genetics; sex determination and differentiation; aquaculture
Special Issues, Collections and Topics in MDPI journals
Dr. Qing-Ping Xie

E-Mail Website
Guest Editor
School of Marine Sciences, Ningbo University, Ningbo, China
Interests: fish; sex determination; sex differentiation; sex-control breeding

Special Issue Information

Dear Colleagues,

With the rapid development of aquaculture around the world, an increasing number of excellent new varieties are urgently needed. Meanwhile, a complete understanding of the range of germplasm resources and rudimentary breeding techniques will be more helpful in mitigating the current problems in fish breeding. This Special Issue will focus on how to retain the wild and farmed genetic resources that underpin global fish production, as well as the applied genetics approaches used for the genetic breeding of fish to increase aquaculture productivity and the conservation of fish genetic resources. Current technology, including genome selective breeding, sex-controlled breeding, and distant hybridization breeding, have shown significant efficiency. Genome selective breeding is one of the most advanced breeding techniques; on the other hand, many fish exhibit sexual dimorphism and new genes (or conventional genes with some potential undiscovered functions), and they are related to sex determination pathways that are yet to be explored. The aim of this Special Issue is to deepen our understanding of the theory and practice of fish genome selective breeding and the mechanisms of sex determination, as well as the analysis of hybridization traits.

Dr. Li-Min Wu
Dr. Dong-Neng Jiang
Dr. Qing-Ping Xie
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fishes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • germplasm resources
  • genetic breeding
  • fish
  • sex control breeding
  • gonadal differentiation

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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Research

14 pages, 9625 KiB  
Article
Mutation of Genes Associated with Body Color, Growth, Intermuscular Bone, and Sex Differentiation in Onychostoma macrolepis Using CRISPR/Cas9
by Tian Gao, Feilong Wang, Qihui Wu, Lingyao Gan, Canbiao Jin, Li Ma, Deshou Wang and Lina Sun
Fishes 2025, 10(2), 40; https://doi.org/10.3390/fishes10020040 - 22 Jan 2025
Viewed by 902
Abstract
Onychostoma macrolepis is not only a protected Cyprinid species in the wild but also an emerging commercial aquaculture fish in China. The objective of this research was to genetically modify the genes associated with commercial traits by CRISPR/Cas9 for the protection and utilization [...] Read more.
Onychostoma macrolepis is not only a protected Cyprinid species in the wild but also an emerging commercial aquaculture fish in China. The objective of this research was to genetically modify the genes associated with commercial traits by CRISPR/Cas9 for the protection and utilization of the germplasm resources of O. macrolepis. To that end, one-cell stage embryos were obtained via hormone-induced ovulation and artificial insemination in O. macrolepis. Eight genes related to body color, growth, intermuscular bone, and sex differentiation were mutated in O. macrolepis using the CRISPR/Cas9 system by microinjection of gRNA/Cas9 mRNA. The optimal dose of gRNA/Cas9 mRNA was determined by injection of different concentrations of tyr (tyrosinase)-gRNA/Cas9 and examination of the mutation rate and hatching rate of embryos. Indels were detected by T7 endonuclease I digestion and Sanger sequencing. F0 mutants with high mutation rates were selected for phenotype analyses. Disruption of body color gene tyr, mpv17 (mitochondrial inner membrane protein MPV17), and csf1ra (colony-stimulating factor 1 receptor, a) resulted in obvious phenotype with decreased or even absence of melanophores, iridophores, and xanthophores, respectively. Mutation of mstnb (myostatin b) led to improved growth performance. Mutation of mc4r (melanocortin 4 receptor) led to no obvious phenotype. Mutation of runx2b (RUNX family transcription factor 2b) and bmp6 (bone morphogenetic protein 6) resulted in decreased or absence of intermuscular bones, as revealed by alizarin red S staining. Mutation of cyp19a1a (cytochrome P450, family 19, subfamily A, polypeptide 1a) resulted in ovarian degeneration as revealed by gonadal histological examination. Therefore, this study successfully obtained mutants with obvious phenotypes of genes associated with body color, growth, intermuscular bone, and sex differentiation by CRISPR/Cas9 in O. macrolepis. Full article
(This article belongs to the Special Issue Advances in Germplasm Resources and Genetic Breeding of Aquatic Animals)
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13 pages, 6946 KiB  
Article
Antibody Preparation, Protein Expression, and Function Analysis of Cyp19a1b in Ovarian Differentiation in a Natural Triploid Teleost Qi River Crucian Carp (Carassius auratus)
by Yongjing Li, Yujiao Jin, Limin Wu and Xuejun Li
Fishes 2024, 9(11), 442; https://doi.org/10.3390/fishes9110442 - 31 Oct 2024
Cited by 1 | Viewed by 1020
Abstract
Estrogen is an essential sex steroid that functions in numerous biological systems including female reproduction, neuroendocrine, vascular, skeletal, and immune systems. The synthesis of estrogen is controlled by the rate-limiting enzyme, which has been confirmed to exist in two different forms, named brain [...] Read more.
Estrogen is an essential sex steroid that functions in numerous biological systems including female reproduction, neuroendocrine, vascular, skeletal, and immune systems. The synthesis of estrogen is controlled by the rate-limiting enzyme, which has been confirmed to exist in two different forms, named brain aromatase and ovary aromatase, and encoded by cyp19a1a and cyp19a1b respectively in teleosts. However, existing studies have primarily focused on the expression and function of cyp19a1b in the brain and cyp19a1a in the gonad, the roles of cyp19a1b in the female gonad of teleosts are largely unknown. In our previous study, we cloned the full length of the cyp19a1b gene from a natural triploid teleost Qi River crucian carp (Carassius auratus), andthe spatial and temporal expression patterns of cyp19a1b mRNA were detected. To further clarify the roles of cyp19a1b in the ovarian differentiation of Qi River crucian carp, we produced a polyclonal antibody of Cyp19a1b in this study. Western blotting results showed that Cyp19a1b was mainly expressed in the brain and then in the ovary, heart, liver, and muscle. During embryogenesis, Cyp19a1b was abundantly expressed in the neurula stage. Immunohistochemistry demonstrated that Cyp19a1b was expressed in the radioactive glial cells (RGCs) of the brain from 20 days after hatching (dah) and the somatic cells of the ovaries from 30 dah, the critical period of ovarian differentiation in Qi River crucian carp. With the treatment of letrozole, an inhibitor of the aromatase, the expression of Cyp19a1b was downregulated both in the brain and gonad. Our results suggested that Cyp19a1b might be involved in the development of the nervous system and also participate in the ovarian differentiation of Qi River crucian carp. Full article
(This article belongs to the Special Issue Advances in Germplasm Resources and Genetic Breeding of Aquatic Animals)
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