Germplasm Resources and Genetic Breeding of Aquatic Animals

A special issue of Fishes (ISSN 2410-3888). This special issue belongs to the section "Genetics and Biotechnology".

Deadline for manuscript submissions: 15 November 2025 | Viewed by 955

Special Issue Editors

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
Interests: fish; genetic; breeding
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Guest Editor
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
Interests: sperm cryopreservation; gynogenesis; selective breeding; crossbreeding; sex control; vitrification cryopreservation for frozen embryos; flounder breeding system; Paralichthys olivaceus; fish

Special Issue Information

Dear Colleagues,

Multifarious taxonomically diverse groups are detected in aquatic animals. Their remarkable evolutionary success is characterized by varied reproductive strategies and multiple genome duplication events throughout their evolutionary history. Investigations into germplasm resources and the genetic breeding of aquatic animals will provide critical insights for understanding growth and development, population diversity, evolutionary trajectories, and molecular breeding applications. Current genetic breeding technologies employed to combat species degeneration encompass hybridization, gynogenesis, androgenesis, selective breeding, transgenic modification, and gene editing technologies. These interventions frequently yield progeny with significant enhancements in commercially important traits including growth performance, survival rates, disease resistance, body color, and environmental stress tolerance. Through advanced genomic approaches, including genome-wide association studies (GWAS), QTL-seq analysis, and transcriptome sequencing, researchers have successfully identified key candidate genes and genetic markers associated with economically valuable traits.

This Special Issue aims to share the latest research trends and applications of “Germplasm Resources and Genetic Breeding of Aquatic Animals” that might shed light on genomic evolution, regulatory mechanism, functional gene, and novel breeding strategies. To advance the knowledge of such intricate issues, contributions from experts in the field, in the form of research papers and critical reviews, are called for.

Dr. Yang Liu
Prof. Dr. Yongsheng Tian
Guest Editors

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Keywords

  • germplasm preservation and utilization
  • genome evolution
  • omics analysis
  • economic traits
  • functional gene
  • mitochondrial genome
  • microsatellite
  • growth and development

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Published Papers (2 papers)

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Research

15 pages, 12128 KB  
Article
Metabolomic and Transcriptomic Analyses of Soft-Body Coloration in Jinjiang Oyster (Crassostrea ariakensis)
by Zhuanzhuan Li, Shuqi Zhao, Jianing Yu, Biao Wu, Peizhen Ma, Xiujun Sun, Liqing Zhou and Zhihong Liu
Fishes 2025, 10(10), 499; https://doi.org/10.3390/fishes10100499 - 3 Oct 2025
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Abstract
The coloration of shellfish significantly influences both environmental adaptability and economic value. In the Jinjiang oyster (Crassostrea ariakensis), soft-body color varies between individuals, with an orange-yellow phenotype distinct from the milky white coloration of the wild type. To elucidate the compositional [...] Read more.
The coloration of shellfish significantly influences both environmental adaptability and economic value. In the Jinjiang oyster (Crassostrea ariakensis), soft-body color varies between individuals, with an orange-yellow phenotype distinct from the milky white coloration of the wild type. To elucidate the compositional differences and molecular mechanisms underlying orange-yellow (designated as CaR) versus milky white (CaW) soft-body color in C. ariakensis, we conducted comparative ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) non-targeted and transcriptomic analyses. A total of 280 differential accumulation metabolites (DAMs) and 691 differentially expressed genes (DEGs) were detected between the CaR and CaW groups. The metabolite set enrichment analysis (MSEA) revealed that DAMs were significantly enriched in pigment metabolism pathways, including tyrosine metabolism, porphyrin metabolism, and lipid metabolism. Furthermore, genes associated with melanin synthesis and carotenoids conversions or transports were upregulated in the CaR vs. CaW group. These genes included Cyp4z1, Cyp4f22, Cyp17a1, Cyp1a5, Cyp2d28a, Lrp4, Aldh, and Tyr-3, potentially driving the accumulation of pheomelanin and carotenoids. This study demonstrates the vital roles of melanin and carotenoid metabolism in Jinjiang oyster body color formation, providing key insights into the molecular mechanisms of color determination in shellfish. Full article
(This article belongs to the Special Issue Germplasm Resources and Genetic Breeding of Aquatic Animals)
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15 pages, 1849 KB  
Article
Embryonic and Larval Development of an Interspecific Grouper Backcross Between the Kelp Grouper (Epinephelus moara) ♀ and KGGG (E. moara ♀ × E. lanceolatus ♂) ♂
by Yidan Xu, Yongsheng Tian, Shihao Wang, Tangtang Ding, Linna Wang, Zhentong Li, Linlin Li and Yang Liu
Fishes 2025, 10(10), 488; https://doi.org/10.3390/fishes10100488 - 1 Oct 2025
Viewed by 295
Abstract
Backcrossing serves as an effective tool for interspecific gene introgression, facilitating germplasm improvement. To develop superior grouper aquaculture varieties, in this study, a backcrossed breed was constructed by crossing a male hybrid grouper, KGGG (Epinephelus moara ♀ × E. lanceolatus ♂), with [...] Read more.
Backcrossing serves as an effective tool for interspecific gene introgression, facilitating germplasm improvement. To develop superior grouper aquaculture varieties, in this study, a backcrossed breed was constructed by crossing a male hybrid grouper, KGGG (Epinephelus moara ♀ × E. lanceolatus ♂), with a female parent kelp grouper (KG; E. moara). Here, we observed embryonic, larval development, and morphological changes in KG × KGGG. Additionally, the total length and body weight of the backcrossed breed and maternal parent were compared at 160 d post hatching (dph). The results showed that fertilization and the hatching rate were 73.23 ± 4.23% and 51.74 ± 3.67%, respectively, and the egg size was 0.89 ± 0.03 mm. Hatching occurred 23:19 h after fertilization at 25 ± 1 °C, and the newly hatched larvae were 1.94 ± 0.13 mm in length. Furthermore, at 160 dph, the total length and body weight of the backcrossed breed were 1.2-fold and 1.9-fold greater, respectively, when compared with those of the maternal parent. Importantly, it is possible that backcrossed KG × KGGG could become a dominant strain in grouper aquaculture practices. Full article
(This article belongs to the Special Issue Germplasm Resources and Genetic Breeding of Aquatic Animals)
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