Strategies for Optimal Fermentation by Using Modern Tools and Methods, 2nd Edition

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Fermentation Process Design".

Deadline for manuscript submissions: 15 September 2025 | Viewed by 871

Special Issue Editor


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Guest Editor
Institute of Microbiology Bulgarian Academy of Sciences, Sofia, Bulgaria
Interests: microalgae; lipids; cultivation; kinetics modeling; photobioreactors modeling; downstream; biodiesel; industrial-scale biodiesel production
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Special Issue Information

Dear Colleagues,

The optimization of fermentation processes to produce biomass, fuels, and chemicals has always faced challenges. The current state of the art highlights the complexity of achieving success in all areas of fermentation, emphasizing that success cannot be achieved without first applying methods that isolate and identify microorganisms from habitats or communities or improve the function of existing microorganisms. Notably, special attention will be given to the following:

  1. Manuscripts on the identification of microorganisms by target amplification methods and nucleic acid sequence analysis;
  2. Nanoparticles as a tool to enhance mass transfer processes and metabolic control during fermentation;
  3. Innovative culturing techniques to facilitate fermentation processes;
  4. The development of subsystem techniques involving microbial kinetics, liquid flow distribution in bioreactors, and heat and mass transfers.

Additionally, manuscripts containing innovative models, response surface analysis, and artificial intelligence-based neural networks to facilitate and improve process design, optimization, control, and large-scale applications are welcome.

Prof. Dr. Alexander Dimitrov Kroumov
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fermentation process
  • metabolic control
  • process modeling
  • design
  • optimization

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Published Papers (1 paper)

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Research

13 pages, 1412 KiB  
Article
Harnessing Raman Spectroscopy for Enhanced Bioprocess Monitoring: Predictive CO2 Analysis and Robust pH Determination in Bioreactor Off-Gas Stream
by Tobias Wallocha and Michaela Poth
Fermentation 2025, 11(6), 317; https://doi.org/10.3390/fermentation11060317 - 2 Jun 2025
Viewed by 660
Abstract
The accurate measurement of CO2 concentration in fermentation off-gas is crucial for monitoring and optimizing bioprocesses, particularly in mammalian cell cultures. In this study, we successfully utilized Raman off-gas spectroscopy to achieve time-resolved prediction of CO2 concentrations in the fermentation off-gas. [...] Read more.
The accurate measurement of CO2 concentration in fermentation off-gas is crucial for monitoring and optimizing bioprocesses, particularly in mammalian cell cultures. In this study, we successfully utilized Raman off-gas spectroscopy to achieve time-resolved prediction of CO2 concentrations in the fermentation off-gas. Our experiments were conducted using two different media: a commercial medium (medium 1) and an in-house Roche medium (medium 2), each tested with two different lots. The results demonstrated that Raman spectroscopy provides precise and real-time CO2 measurements, which are essential for effective process monitoring and control. Furthermore, we established that CO2 off-gas analysis can be directly correlated with the pH value of the fermentation medium. This correlation allows for accurate pH prediction with comparable precision to traditional methods, where CO2 levels are first determined via Raman spectroscopy or an off-gas analyzer and then used to infer pH through a correlation curve. In the final step of our study, we employed a Raman submers probe to predict CO2 and pH directly within the fermentation medium. Compared to the model accuracy in the off-gas stream, the performance of the Raman submers probe in predicting CO2 and pH within the medium was significantly worse, likely due to the absence of a pretrained model for CO2. Our findings highlight the potential of Raman off-gas spectroscopy as a powerful tool for real-time bioprocess monitoring and control, offering significant advantages in terms of accuracy and efficiency. Full article
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