ELISPOT Research

Dear Colleagues,

ELISPOT is a technique of choice for the detection of spontaneous and antigen-induced secretion of cytokines (e.g. IFN-gamma, TNF-alpha, IL-2, IL-4, etc.) from peripheral blood lymphocytes. This assay is widely used for vaccine development, AIDS research, cancer research, infectious diseases monitoring, autoimmune disease studies, and allergy and transplantation research. Due to its unsurpassed sensitivity, the areas of ELISPOT application are rapidly growing and expanding into human and veterinary diagnostics, vaccine development, drug screening, and as a companion assay for high content screening. The growing number of publications attests towards the recognition that ELISPOT technique is an indispensable tool for both basic research and diagnostic studies and is strong evidence that this technique is a must-use tool in modern day biomedical research. Due to its inherent nature, ELISPOT is one of the most flexible assays and can be endlessly modified and adapted to particular experimental needs of the investigator. This Special Issue offers an Open Access forum at bringing together a collection of novel protocols, methods and techniques that can be shared among investigators from different laboratories. We encourage novices and experienced scientists to contribute research papers and reviews dedicated to analysis of secretion of novel cytokines, multiplex ELSIPOT techniques using chromogenic and fluorescence detection, statistical analysis, novel cell-treatment strategies, high-sensitivity detection protocols, T-cell and B-cell ELISPOT assays, vaccine development, diagnostics and drug discovery research. ELISPOT has enormous potential for development and expansion into new research areas and our goal is to help advancing in this direction.

Prof. Dr. Alexander E. Kalyuzhny
Guest Editor

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