Increased Level of IFN-γ and IL-4 Spot-Forming Cells on ELISPOT Assay as Biomarkers for Acute Graft-Versus-Host Disease and Concurrent Infections
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Characteristics
Characteristic | aGVHD grade 0 (n = 49) | aGVHD grade I-IV (n = 31) | P | |
---|---|---|---|---|
Median age (range) | 15 (1–61) | 14 (1–49) | NS* | |
Male: Female | 33:16 | 19:12 | NS# | |
Donor type | ||||
Related | 23 | 13 | NS# | |
Unrelated | 26 | 18 | ||
Stem cell source | ||||
Bone marrow | 37 | 22 | ||
Peripheral blood | 5 | 5 | NS† | |
Cord blood | 7 | 4 | ||
GVHD prophylaxis | ||||
Cyclosporin A ± MTX | 40 | 24 | NS# | |
Tacrolimus ± MTX | 9 | 7 | ||
Conditioning regimen | ||||
TBI based | 26 | 19 | ||
BU based | 15 | 9 | NS† | |
Others | 8 | 3 | ||
HLA histocompatibility | ||||
6/6 | 38 | 26 | ||
5/6 | 6 | 4 | NS† | |
4/6, 3/6 | 5 | 1 | ||
Pretransplant CMV serostatus | ||||
(Donor/recipient) | ||||
+/+ | 24 | 14 | NS† | |
−/+ | 9 | 8 | ||
+/− | 11 | 4 | ||
−/− | 5 | 5 | ||
Posttransplant CMV antigenemia | ||||
Positive | 16 | 10 | NS# | |
Negative | 33 | 21 |
2.2. Evaluation of Events and Sample Collection
2.3. ELISA
2.4. ELISPOT Assay
2.5. Statistical Analysis
3. Results
3.1. Spot-Forming Cells in Patients Who Underwent Allogeneic HSCT
3.2. Plasma Cytokine Levels in Patients Who Underwent Allogeneic HSCT
3.3. ELISPOT Assay Discriminates aGVHD from Infection
4. Discussion
5. Conclusions
Acknowledgments
Conflict of Interest
References
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Hirayama, M.; Azuma, E.; Komada, Y. Increased Level of IFN-γ and IL-4 Spot-Forming Cells on ELISPOT Assay as Biomarkers for Acute Graft-Versus-Host Disease and Concurrent Infections. Cells 2012, 1, 61-73. https://doi.org/10.3390/cells1020061
Hirayama M, Azuma E, Komada Y. Increased Level of IFN-γ and IL-4 Spot-Forming Cells on ELISPOT Assay as Biomarkers for Acute Graft-Versus-Host Disease and Concurrent Infections. Cells. 2012; 1(2):61-73. https://doi.org/10.3390/cells1020061
Chicago/Turabian StyleHirayama, Masahiro, Eiichi Azuma, and Yoshihiro Komada. 2012. "Increased Level of IFN-γ and IL-4 Spot-Forming Cells on ELISPOT Assay as Biomarkers for Acute Graft-Versus-Host Disease and Concurrent Infections" Cells 1, no. 2: 61-73. https://doi.org/10.3390/cells1020061
APA StyleHirayama, M., Azuma, E., & Komada, Y. (2012). Increased Level of IFN-γ and IL-4 Spot-Forming Cells on ELISPOT Assay as Biomarkers for Acute Graft-Versus-Host Disease and Concurrent Infections. Cells, 1(2), 61-73. https://doi.org/10.3390/cells1020061