Spatial Biology: Decoding Cellular Complexity in Tissues

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Tissues and Organs".

Deadline for manuscript submissions: 30 April 2026 | Viewed by 1087

Special Issue Editor


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Guest Editor

Special Issue Information

Dear Colleagues,

Spatial biology (SB), also known as spatial tissue profiling, is a new and rapidly advancing discipline. Being a descendent of traditional multiplex immunohistochemistry, SB far exceeds the latter in multiplexing capacity: it allows for interrogating hundreds of genes and proteins in a single histological tissue sample, shedding light on the in situ localization of biomarkers and their interactions within the tissue microenvironment.

To mark the importance of the field of SB and address the needs of researchers to share their latest achievements, we are launching a Special Issue entitled “Spatial Biology: Decoding Cellular Complexity in Tissues”. This Special Issue will present research articles and high-quality review papers in the spatial biology/spatial tissue profiling research fields. We encourage researchers working in proteomics and transcriptomics, as well as other cell and tissue research areas, to make contributions to this Special Issue.

Dr. Alexander E. Kalyuzhny
Guest Editor

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Keywords

  • spatial biology
  • spatial tissue profiling
  • proteomics
  • transcriptomics
  • cell and tissue

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Published Papers (1 paper)

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Research

14 pages, 37937 KB  
Communication
Unlocking the Tumor Microenvironment: Innovations in Multiplex Immunohistochemistry
by Bipin Gupta, George Yang and Marc Key
Cells 2025, 14(22), 1819; https://doi.org/10.3390/cells14221819 - 20 Nov 2025
Viewed by 660
Abstract
The immune control of cancer growth is an area of active investigation. In this study, we demonstrated the feasibility of using standard immunohistochemistry methods in conjunction with a set of newly developed chromogens to demonstrate immune cell markers in a multiplex staining system. [...] Read more.
The immune control of cancer growth is an area of active investigation. In this study, we demonstrated the feasibility of using standard immunohistochemistry methods in conjunction with a set of newly developed chromogens to demonstrate immune cell markers in a multiplex staining system. Immune infiltrating cells in breast cancer were identified using antibodies to CD20 (B-cells), CD3 (T-cells), and CD163 (macrophages). Additionally, the tumor compartment was identified using cytokeratin (AE1/AE3), and Ki67 was used to determine the proliferation index. These stains showed a significant immune cell infiltrate surrounding and within the tumors. B-cells, T-cells, and macrophages were abundant at the tumor periphery, particularly in areas where tertiary lymphoid structures were also present. In contrast, B-cells were significantly reduced within the tumor interior compared to an abundant infiltrate of T-cells and macrophages. Patterns of B-cell, T-cell, and macrophage infiltration were identified. Depending upon the particular set of markers chosen for analysis, a simple visual examination, without the aid of computer-assisted imaging systems, was sufficient to differentiate up to five different markers. Full article
(This article belongs to the Special Issue Spatial Biology: Decoding Cellular Complexity in Tissues)
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