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Journals
Biosensors
Special Issues
Microarray for Biomarker Detection and Discovery
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Microarray for Biomarker Detection and Discovery

A special issue of Biosensors (ISSN 2079-6374).

Deadline for manuscript submissions: closed (30 April 2020) | Viewed by 8579

Special Issue Editors

Dr. Taisun Kim

E-Mail Website
Guest Editor
Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Republic of Korea
Interests: supramolecular chemistry; microarray; protein chips; lateral flow strip membrane assay
Special Issues, Collections and Topics in MDPI journals
Dr. Satish Balasaheb Nimse

E-Mail Website
Co-Guest Editor
Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 24252, Gangwon-do, Republic of Korea
Interests: medicinal chemistry; bioorganic chemistry; supramolecular chemistry; biosensors; chemosensors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nucleic acid microarrays including DNA chips and lateral flow membrane strips are state-of-the art technologies that allow efficient detection and discrimination of bacteria and viruses. Protein–DNA conjugation chemistry has extended the applications of microarrays for detection and quantification of biomarker proteins. Other applications of microarrays include gene discovery, disease diagnosis, drug discovery, toxicological research, etc. Nonetheless, the demand for high quality microarrays that are robust, applicable in point-of-care settings, easy to use, and more importantly, allow effective mass production is ever increasing. Therefore, this Special Issue "Microarray for Biomarker Detection and Discovery" focuses on the recent advances in the production of DNA chips, lateral flow membrane strips, and their applications in the detection and discovery of biomarkers (DNA, RNA, antigen, antibody, small molecules). We invite submissions of research that help to advance the field of microarray technology and its application for high-throughput analysis of biomarkers.

With best regards,

Prof. Dr. Taisun Kim
Prof. Dr. Satish Balasaheb Nimse
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microarray
  • bacteria, viruses
  • biomarkers
  • DNA
  • RNA
  • hybridization
  • antigen
  • antibody
  • small molecules

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Published Papers (1 paper)

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Research

14 pages, 5322 KiB  
Article
Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay
by Marc Prante, Torsten Schüling, Bernhard Roth, Kort Bremer and Johanna Walter
Biosensors 2019, 9(4), 134; https://doi.org/10.3390/bios9040134 - 13 Nov 2019
Cited by 29 | Viewed by 7547
Abstract
Detection of the small molecule 25-hydroxyvitamin D (25(OH)D) as the most relevant marker for vitamin D supply suffers from a high variability of results using the current detection methods, such as high-performance liquid chromatography (HPLC) and immunoassays. A new detection approach using a [...] Read more.
Detection of the small molecule 25-hydroxyvitamin D (25(OH)D) as the most relevant marker for vitamin D supply suffers from a high variability of results using the current detection methods, such as high-performance liquid chromatography (HPLC) and immunoassays. A new detection approach using a highly specific aptamer directed against 25(OH)D was established in this study based on the target-induced dissociation (TID) sensing approach. In this work, the aptamer was investigated regarding its structural properties as well as its binding affinity by using microscale thermophoresis (MST). Moreover, complementary oligonucleotides were designed based on the aptamer structure and were evaluated in MST experiments. Binding experiments of immobilized aptamers were conducted in microarray experiments. It could be shown that the aptamer exhibited the usual B-DNA structure and did not form any G-quadruplexes. The design of complementary oligonucleotides for the TID assay identified a putative 25(OH)D binding site within the aptamer. The limit of detection of the established competitive assay was determined to be 5.4 nM, which sets the stage for the development of a biosensor system. Full article
(This article belongs to the Special Issue Microarray for Biomarker Detection and Discovery)
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