Special Issue "Application of Nanomaterials for Biosensors"

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: 31 August 2022 | Viewed by 5324

Special Issue Editors

Dr. Yaoguang Wang
E-Mail Website
Guest Editor
School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
Interests: electrochemical biosensors; biomarker detection; environmental analysis; nanomaterials; nanozymes; nanotechnology
Dr. Shakil Awan
E-Mail Website
Guest Editor
Wolfson Nanomaterials and Devices Laboratory, School of Computing, Electronics and Mathematics, Faculty of Science and Engineering, University of Plymouth, Plymouth PL4 8AA, UK
Interests: Alzheimer’s disease biomarker detection; electrochemical biosensors; graphene and 2D materials; high-speed and quantum electronics; metrology; superconductivity

Special Issue Information

Dear Colleagues,

The study of biosensors is an interdisciplinary subject that organically combines bioactive materials (enzymes, proteins, DNA, antibodies, antigens, biofilms, etc.) with physical and chemical transducers. Biosensors represent an essential advanced detection method and monitoring method for the development of biotechnology, as well as a rapid trace analysis method at the molecular level of substances. It has wide application prospects in clinical diagnosis, food and drug analysis, environmental protection, biotechnology, biochips, and more. Nanomaterials, spanning graphene and 2D materials, carbon nanotubes, nanowires, quantum dots etc., are considered to be the hot spot in the field of materials research. The rapid development of nanotechnology provides a rare opportunity for the development of novel biosensors with high sensitivity, specificity, and stability. Therefore, this Special Issue “Application of Nanomaterials for Biosensors” focuses on the recent advances about the application of nanomaterials for fabricating novel biosensors for the detection of disease biomarkers, as well as food and environmental contaminants. We invite the submission of research that helps to advance the field of bionanotechnology and its applications. 

Dr. Yaoguang Wang
Dr. Shakil Awan
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • nanomaterials
  • disease markers
  • food contaminants
  • environmental analysis
  • nanotechnology
  • proteins
  • DNA
  • antibodies
  • antigens

Published Papers (9 papers)

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Research

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Article
A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green
Biosensors 2022, 12(7), 456; https://doi.org/10.3390/bios12070456 - 26 Jun 2022
Viewed by 216
Abstract
In this research, we designed a rapid tricolour immunochromatographic test strip with double test lines (TS-DTL) and two-colour AuNP probes, which realised the simultaneous detection of tricaine mesylate (TMS) and malachite green (MG). Through a distinct tricolour system (red T1 line, blue [...] Read more.
In this research, we designed a rapid tricolour immunochromatographic test strip with double test lines (TS-DTL) and two-colour AuNP probes, which realised the simultaneous detection of tricaine mesylate (TMS) and malachite green (MG). Through a distinct tricolour system (red T1 line, blue T2 line and purple C line), a visual identification of TMS (0.2 μg/mL) and MG (0.5 μg/mL) was quickly achieved on site, which improved the accuracy of naked eye observations. The LODs of TMS in aquaculture water, fish and shrimp were 11.0, 29.6 and 61.4 ng/mL, respectively. MG LODs were 47.0 ng/mL (aquaculture water), 82.8 ng/mL (fish) and 152.4 ng/mL (shrimp). The LOD of MG was close to the similar TS methods. However, visual detection of TMS could meet the requirements of the residue limit (1 μg/mL) of TMS in the USA, and the quantitative detection of TMS was over 16 times lower than the USA standard. The developed platform was rapid (~20 min, HPLC~3 h) and accurate, which was verified using a traditional HPLC method. The recovery rates ranged from 82.2% to 108.6% in three types of real samples, indicating a potential application in on-site fast screening or multiple detection for TMS and MG residues in aquatic products. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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Communication
Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor
Biosensors 2022, 12(7), 444; https://doi.org/10.3390/bios12070444 - 23 Jun 2022
Viewed by 245
Abstract
MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor consisting [...] Read more.
MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor consisting of grapheme oxide modified with a molecular beacon and peptide. The nanosensor can simultaneously detect and image miRNA-221 and apoptotic protein caspase-3 in living cells. Intracellular experiments showed that the nanosensor could be successfully applied for in situ monitoring of the effect of miRNA-221 expression changes on apoptosis by dual-color imaging. The current strategy could provide new avenues for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA and developing personalized treatment plans. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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Article
Solid-Phase Synthesis of Red Fluorescent Carbon Dots for the Dual-Mode Detection of Hexavalent Chromium and Cell Imaging
Biosensors 2022, 12(6), 432; https://doi.org/10.3390/bios12060432 - 20 Jun 2022
Viewed by 317
Abstract
The excellent optical properties and biocompatibility of red fluorescence carbon dots (R-CDs) provide a new approach for the effective analysis of hexavalent chromium Cr(VI) in environmental and biological samples. However, the application of R-CDs is still limited by low yield and unfriendly synthesis [...] Read more.
The excellent optical properties and biocompatibility of red fluorescence carbon dots (R-CDs) provide a new approach for the effective analysis of hexavalent chromium Cr(VI) in environmental and biological samples. However, the application of R-CDs is still limited by low yield and unfriendly synthesis route. In this study, we developed a new type of R-CDs based on a simple and green solid-phase preparation strategy. The synthesized R-CDs can emit bright red fluorescence with an emission wavelength of 625 nm and also have an obvious visible light absorption capacity. Furthermore, the absorption and fluorescence signals of the R-CDs aqueous solution are sensitive to Cr(VI), which is reflected in color change and fluorescence quenching. Based on that, a scanometric and fluorescent dual-mode analysis system for the rapid and accurate detection of Cr(VI) was established well within the limit of detection at 80 nM and 9.1 nM, respectively. The proposed methods also possess high specificity and were applied for the detection of Cr(VI) in real water samples. More importantly, the synthesized R-CDs with good biocompatibility were further successfully applied for visualizing intracellular Cr(VI) in Hela cells. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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Article
An Efficient Enzyme-Less Uric Acid Sensor Development Based on PbO-Doped NiO Nanocomposites
Biosensors 2022, 12(6), 381; https://doi.org/10.3390/bios12060381 - 31 May 2022
Viewed by 494
Abstract
Here, the voltammetric electrochemical approach was applied to detect uric acid (UA) in a conductive sensing medium (phosphate buffer solution-PBS) by using PbO-doped NiO nanocomposites (NCs)-decorated glassy carbon electrode (GCE) performing as working electrode. The wet-chemically prepared PbO-doped NiO NCs were subjected to [...] Read more.
Here, the voltammetric electrochemical approach was applied to detect uric acid (UA) in a conductive sensing medium (phosphate buffer solution-PBS) by using PbO-doped NiO nanocomposites (NCs)-decorated glassy carbon electrode (GCE) performing as working electrode. The wet-chemically prepared PbO-doped NiO NCs were subjected to characterization by the implementation of XRD, FESEM, XPS, and EDS analysis. The modified GCE was used to detect uric acid (UA) in an enzyme-free conductive buffer (PBS) of pH = 7.0. As the outcomes of this study reveal, it exhibited good sensitivity of 0.2315 µAµM−1cm−2 and 0.2233 µAµM−1cm−2, corresponding to cyclic (CV) and differential pulse (DPV) voltammetric analysis of UA, respectively. Furthermore, the proposed UA sensor showed a wider detection (0.15~1.35 mM) range in both electrochemical analysis methods (CV & DPV). In addition, the investigated UA sensor displayed appreciable limit of detection (LOD) of 41.0 ± 2.05 µM by CV and 43.0 ± 2.14 µM by DPV. Good reproducibility performance, faster response time and long-time stability in detection of UA were perceived in both electrochemical analysis methods. Finally, successful analysis of the bio-samples was performed using the recovery method, and the results were found to be quite acceptable in terms of accuracy. Thus, the findings indicate a reliable approach for the development of 5th generation biosensors using metal-oxides as sensing substrate to fulfill the requirements of portable use for in situ detection. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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Article
Acetone Gas Sensor Based on SWCNT/Polypyrrole/Phenyllactic Acid Nanocomposite with High Sensitivity and Humidity Stability
Biosensors 2022, 12(5), 354; https://doi.org/10.3390/bios12050354 - 19 May 2022
Viewed by 559
Abstract
We synthesized core-shell-shaped nanocomposites composed of a single-walled carbon nanotube (SWCNT) and heptadecafluorooctanesulfonic acid-doped polypyrrole (C8F-doped-PPy)/phenyllatic acid (PLA), i.e., C8F-doped-PPy/[email protected], for detecting acetone gas with high sensitivity and humidity stability. The obtained nanocomposites have the structural features of a sensing material as a [...] Read more.
We synthesized core-shell-shaped nanocomposites composed of a single-walled carbon nanotube (SWCNT) and heptadecafluorooctanesulfonic acid-doped polypyrrole (C8F-doped-PPy)/phenyllatic acid (PLA), i.e., C8F-doped-PPy/[email protected], for detecting acetone gas with high sensitivity and humidity stability. The obtained nanocomposites have the structural features of a sensing material as a C8F-doped-PPy layer surrounding a single-stranded SWCNT, and a PLA layer on the outer surface of the PPy as a specific sensing layer for acetone. PLA was chemically combined with the positively charged PPy backbone and provided the ability to reliably detect acetone gas at concentrations as low as 50 ppb even at 25 °C, which is required for medical diagnoses via human breath analysis. When C8F was contained in the pyrrole monomer in a ratio of 0.1 mol, it was able to stably detect an effective signal in a relative humidity (RH) of 0–80% range. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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Article
Multi-Functional Silver Nanoparticles for High-Throughput Endospore Sensing
Biosensors 2022, 12(2), 68; https://doi.org/10.3390/bios12020068 - 25 Jan 2022
Viewed by 864
Abstract
In spore-forming bacteria such as Bacillus and Clostridium, the vegetative cells form highly durable hard shells called endospores inside the bacteria to survive as the growth environment deteriorates. Because of these properties, endospores can cause food poisoning and medical accidents if they [...] Read more.
In spore-forming bacteria such as Bacillus and Clostridium, the vegetative cells form highly durable hard shells called endospores inside the bacteria to survive as the growth environment deteriorates. Because of these properties, endospores can cause food poisoning and medical accidents if they contaminate food, medicine, or other products, and it is required for technology to detect the spores at the manufacturing site. In this study, we focused on the surface-enhanced Raman scattering (SERS) method for the sensitive detection of dipicolinic acid (DPA), a molecular marker of endospores. We constructed Fe3O4/Ag core–shell functional silver nanoparticles that specifically bind to DPA, and investigated a method for the qualitative detection of DPA by SERS and the quantitative detection of DPA by fluorescence method using a terbium complex formed on the surface. As a result, the concentration of the functional silver nanoparticles constructed could detect spore-derived DPA by fluorescence detection method, and SERS was several tens of nM. The functionalized nanoparticles can detect DPA quantitatively and qualitatively, and are expected to be applied to detection technology in the production of food and pharmaceuticals. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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Article
Ultrasensitive Photochemical Immunosensor Based on Flowerlike SnO2/BiOI/Ag2S Composites for Detection of Procalcitonin
Biosensors 2021, 11(11), 421; https://doi.org/10.3390/bios11110421 - 28 Oct 2021
Cited by 2 | Viewed by 683
Abstract
Based on the necessity and urgency of detecting infectious disease marker procalcitonin (PCT), a novel unlabeled photoelectrochemical (PEC) immunosensor was prepared for the rapid and sensitive detection of PCT. Firstly, SnO2 porous nanoflowers with good photocatalytic performance were prepared by combining hydrothermal [...] Read more.
Based on the necessity and urgency of detecting infectious disease marker procalcitonin (PCT), a novel unlabeled photoelectrochemical (PEC) immunosensor was prepared for the rapid and sensitive detection of PCT. Firstly, SnO2 porous nanoflowers with good photocatalytic performance were prepared by combining hydrothermal synthesis and calcining. BiOI nanoflowers were synthesized by facile ultrasonic mixed reaction. Ag2S quantum dots were deposited on SnO2/BiOI composites by in situ growth method. The SnO2/BiOI/Ag2S composites with excellent photoelectric properties were employed as substrate material, which could provide significantly enhanced and stable signal because of the energy level matching of SnO2, BiOI and Ag2S and the good light absorption performance. Accordingly, a PEC immunosensor based on SnO2/BiOI/Ag2S was constructed by using the layered modification method to achieve high sensitivity analysis of PCT. The linear dynamic range of the detection method was 0.50 pg·mL−1~100 ng·mL−1, and the detection limit was 0.14 pg·mL−1. In addition, the designed PEC immunosensor exhibited satisfactory sensitivity, selectivity, stability and repeatability, which opened up a new avenue for the analyzation of PCT and further provided guidance for antibiotic therapy. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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Review

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Review
Recent Advances of Biochar-Based Electrochemical Sensors and Biosensors
Biosensors 2022, 12(6), 377; https://doi.org/10.3390/bios12060377 - 31 May 2022
Viewed by 533
Abstract
In the context of accelerating the global realization of carbon peaking and carbon neutralization, biochar produced from biomass feedstock via a pyrolysis process has been more and more focused on by people from various fields. Biochar is a carbon-rich material with good properties [...] Read more.
In the context of accelerating the global realization of carbon peaking and carbon neutralization, biochar produced from biomass feedstock via a pyrolysis process has been more and more focused on by people from various fields. Biochar is a carbon-rich material with good properties that could be used as a carrier, a catalyst, and an absorbent. Such properties have made biochar a good candidate as a base material in the fabrication of electrochemical sensors or biosensors, like carbon nanotube and graphene. However, the study of the applications of biochar in electrochemical sensing technology is just beginning; there are still many challenges to be conquered. In order to better carry out this research, we reviewed almost all of the recent papers published in the past 5 years on biochar-based electrochemical sensors and biosensors. This review is different from the previously published review papers, in which the types of biomass feedstock, the preparation methods, and the characteristics of biochar were mainly discussed. First, the role of biochar in the fabrication of electrochemical sensors and biosensors is summarized. Then, the analytes determined by means of biochar-based electrochemical sensors and biosensors are discussed. Finally, the perspectives and challenges in applying biochar in electrochemical sensors and biosensors are provided. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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Review
Research Progress of Graphene and Its Derivatives towards Exhaled Breath Analysis
Biosensors 2022, 12(2), 48; https://doi.org/10.3390/bios12020048 - 18 Jan 2022
Viewed by 879
Abstract
The metabolic process of the human body produces a large number of gaseous biomarkers. The tracking and monitoring of certain diseases can be achieved through the detection of these markers. Due to the superior specific surface area, large functional groups, good optical transparency, [...] Read more.
The metabolic process of the human body produces a large number of gaseous biomarkers. The tracking and monitoring of certain diseases can be achieved through the detection of these markers. Due to the superior specific surface area, large functional groups, good optical transparency, conductivity and interlayer spacing, graphene, and its derivatives are widely used in gas sensing. Herein, the development of graphene and its derivatives in gas-phase biomarker detection was reviewed in terms of the detection principle and the latest detection methods and applications in several common gases, etc. Finally, we summarized the commonly used materials, preparation methods, response mechanisms for NO, NH3, H2S, and volatile organic gas VOCs, and other gas detection, and proposed the challenges and prospective applications in this field. Full article
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
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