Fluorescence-Based Sensors for Bio-Analysis: Current Advances, Gaps, and Future Outlook
A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".
Deadline for manuscript submissions: 31 August 2026 | Viewed by 276
Special Issue Editor
Special Issue Information
Dear Colleagues,
Fluorescence-based techniques have become dominant in the field of bioimaging because of their high sensitivity and selectivity. They enable the real-time detection of bioanalytes with spatio-temporal precision, revealing the intricate molecular details of complex biological processes in living cells and organisms. This has been possible with the help of a number of strategies such as fluorescence turn-on probes, ratiometric sensing, FRET, smFRET, BRET, and fluorescence lifetime-based approaches. Biosensors can be designed using fluorescent proteins (FPs), small-molecule fluorescent organic dyes, and nanomaterials. Because of the versatile nature of fluorescence techniques, these fluorescence-based approaches are highly effective for detecting diverse analytes, including small molecules, metabolites, metal ions, proteins, DNA, and RNA. Therefore, fluorescence-based biosensing has attracted significant attention across the fields of biomedical and life sciences.
For this Special Issue, we invite original research manuscripts focused on the following: (1) The design and development of novel fluorescence probes (smFRET, BRET, FLIM-FRET, FRET, turn-on, ratiometric, lifetime) for the targeted detection of bioanalytes such as metal ions, lipids, proteins, metabolites, and small molecules. (2) The application of biosensors in diagnostics and the real-time monitoring of cellular dynamics and biological processes. (3) Multiplexed imaging with biosensors. Articles can also explore the intersections of the topics discussed above. Additionally, review articles covering recent progress, research gaps, and future outlooks in the domain of biosensors—particularly those categorically addressing the detection of bioanalytes such as ions, small molecules and metabolites, proteins, lipids, and enzymes through a specific fluorescence-based technique or a combination of different techniques—are highly encouraged.
Dr. Ananya Rakshit
Guest Editor
Manuscript Submission Information
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Keywords
- fluorescence
- optical sensors design
- smFRET
- BRET
- FLIM-FRET
- FRET sensors
- turn-on probes
- ratiometric sensing
- genetically encoded sensors
- lifetime imaging
- biomolecules detection
- bioanalytes imaging
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