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Biosensors
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Aptamer-Based Biosensing and Bioassays
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Aptamer-Based Biosensing and Bioassays

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: closed (30 September 2024) | Viewed by 6232

Special Issue Editor

Dr. Mahmoud Amouzadeh Tabrizi

E-Mail Website
Guest Editor
Electronic Technology Department, Universidad Carlos III de Madrid, 28911 Leganés, Spain
Interests: biosensors; electrochemistry; point of care devices; nanomaterials; surface modification
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Thanks to advances in science and technology, several artificial recognizers (AFRs) have been synthesized in recent years, opening a new chapter in bioassays and biosensors.

Aptamers are well-known examples of AFRs that are used for the detection of a wide range of targets, from heavy ions to cancer cells. The aptamer is an artificial probe widely used for the fabrication of biosensors for the determination and imaging of molecules, ions, cells, bacteria, etc. Peptide, nucleic acid (DNA and RNA) and peptide nucleic acid (PNA) probes are the most common aptamers used to fabricate aptasensors. Aptamers can selectively interact with their targets, preparing a unique opportunity for the detection of materials of biological phenomena such as cellular communication, mechanical tension forces in cells, etc. The aptamer/target interaction can be detected with the use of several transducers such as electrochemical and optical ones.

Results indicate that through the integration of nanomaterials with aptamers, the analytical performance of sensors such as the limit of detection and linear range can be improved.

This Special Issue intends to collect novel research on sensing technologies with the potential to be used for the fabrication of aptamer-based sensors in real samples, including original papers describing novel biosensors for the detection of biomarkers, cancer cells, bacteria, viruses, DNA or RNA fragments, and water and soil pollution components. Review articles should address recent advances in aptamer sensor technologies.

We look forward to receiving your contributions.

Dr. Mahmoud Amouzadeh Tabrizi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • aptamer
  • nanomaterials
  • biomarkers
  • point-of-care
  • biosensors
  • bioassays

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Published Papers (2 papers)

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14 pages, 2357 KiB  
Article
Comparative Analysis of QCM and Electrochemical Aptasensors for SARS-CoV-2 Detection
by Katarína Nemčeková, Jana Korčeková, Veronika Svitková, Denis Baraniak, Michaela Domšicová, Eva Melníková, Michaela Hornychová, Viktória Szebellaiová, Miroslav Gál and Alexandra Poturnayová
Biosensors 2024, 14(9), 431; https://doi.org/10.3390/bios14090431 - 6 Sep 2024
Cited by 1 | Viewed by 1756
Abstract
The rapid and accurate detection of SARS-CoV-2, particularly its spike receptor-binding domain (S-RBD), was crucial for managing the COVID-19 pandemic. This study presents the development and optimization of two types of aptasensors: quartz crystal microbalance (QCM) and electrochemical sensors, both employing thiol-modified DNA [...] Read more.
The rapid and accurate detection of SARS-CoV-2, particularly its spike receptor-binding domain (S-RBD), was crucial for managing the COVID-19 pandemic. This study presents the development and optimization of two types of aptasensors: quartz crystal microbalance (QCM) and electrochemical sensors, both employing thiol-modified DNA aptamers for S-RBD detection. The QCM aptasensor demonstrated exceptional sensitivity, achieved by optimizing aptamer concentration, buffer composition, and pre-treatment conditions, with a limit of detection (LOD) of 0.07 pg/mL and a linear range from 1 pg/mL to 0.1 µg/mL, and a significant frequency change was observed upon target binding. The electrochemical aptasensor, designed for rapid and efficient preparation, utilized a one-step modification process that reduced the preparation time to 2 h while maintaining high sensitivity and specificity. Electrochemical impedance spectroscopy (EIS) enabled the detection of S-RBD concentrations as low as 132 ng/mL. Both sensors exhibited high specificity, with negligible non-specific interactions observed in the presence of competing proteins. Additionally, the QCM aptasensor’s functionality and stability were verified in biological fluids, indicating its potential for real-world applications. This study highlights the comparative advantages of QCM and electrochemical aptasensors in terms of preparation time, sensitivity, and specificity, offering valuable insights for the development of rapid, sensitive, and specific diagnostic tools for the detection of SARS-CoV-2 and other viruses. Full article
(This article belongs to the Special Issue Aptamer-Based Biosensing and Bioassays)
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24 pages, 761 KiB  
Systematic Review
Are Aptamer-Based Biosensors the Future of the Detection of the Human Gut Microbiome?—A Systematic Review and Meta-Analysis
by Maria João Moreira, Manuela Pintado and José M. M. M. De Almeida
Biosensors 2024, 14(9), 423; https://doi.org/10.3390/bios14090423 - 2 Sep 2024
Cited by 2 | Viewed by 4059
Abstract
The gut microbiome is shaped early in life by dietary and lifestyle factors. Specific compounds in the gut affect the growth of different bacterial species and the production of beneficial or harmful byproducts. Dysbiosis of the gut microbiome has been linked to various [...] Read more.
The gut microbiome is shaped early in life by dietary and lifestyle factors. Specific compounds in the gut affect the growth of different bacterial species and the production of beneficial or harmful byproducts. Dysbiosis of the gut microbiome has been linked to various diseases resulting from the presence of harmful bacteria and their byproducts. Existing methods for detecting microbial species, such as microscopic observation and molecular biological techniques, are costly, labor-intensive, and require skilled personnel. Biosensors, which integrate a recognition element, transducer, amplifier, signal processor, and display unit, can convert biological events into electronic signals. This review provides a comprehensive and systematic survey of scientific publications from 2018 to June 2024, obtained from ScienceDirect, PubMed, and Scopus databases. The aim was to evaluate the current state-of-the-art and identify knowledge gaps in the application of aptamer biosensors for the determination of gut microbiota. A total of 13 eligible publications were categorized based on the type of study: those using microbial bioreceptors (category 1) and those using aptamer bioreceptors (category 2) for the determination of gut microbiota. Point-of-care biosensors are being developed to monitor changes in metabolites that may lead to disease. They are well-suited for use in the healthcare system and offer an excellent alternative to traditional methods. Aptamers are gaining attention due to their stability, specificity, scalability, reproducibility, low production cost, and low immunogenicity. While there is limited research on using aptamers to detect human gut microbiota, they show promise for providing accurate, robust, and cost-effective diagnostic methods for monitoring the gut microbiome. Full article
(This article belongs to the Special Issue Aptamer-Based Biosensing and Bioassays)
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