Submit to Biosensors Review for Biosensors Propose a Special Issue

Journal Menu

Journal Browser

Point-of-Care Detection of COVID-19 and Other Infections Disease: Advancement and Innovations in Biosensing Methods

Special Issue Editors
Special Issue Information
Keywords
Published Papers

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (31 August 2023) | Viewed by 8855

Share This Special Issue

Special Issue Editors

Dr. Sharmili Roy

E-Mail Website
Guest Editor

Division of Oncology, School of Medicine, Stanford University, Palo Alto, CA 94305, USA
Interests: biosensors; molecular technologies; next-generation sequencing

Dr. Pranjal Chandra

E-Mail Website
Guest Editor

School of Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi, India
Interests: bio-physio sensors; nanobioengineering; microfluidics; healthcare technologies; material engineering for diverse applications

Special Issue Information

Dear Colleagues,

Novel human coronavirus, considered as a severe acute respiratory syndrome coronavirus (SARS-CoV-2), had a worldwide outbreak that started in late 2019. This new virus and its mutated strains are continuing to rapidly spread around the world and many deaths are still occurring in every country. Evidence from many researchers has shown that COVID-19 transmission occurs primarlily between individuals, directly through close contact via respiratory droplets during coughing, sneezing and even while talking. Considering this emergency, point-of-care (POC) detection of an infected person and isolating those individuals is very much important, and needs to be achieved as quickly as possible. Hence, biosensor and biosensing on-the-spot devices are mainly in high demand for rapid testing of COVID-19 for safe and healthy indibiduals. Biosensors also can make things easier for healthcare personnel, so that they do not have to carry infected samples to the laboratory, since these devices can detect the virus within few mintues on-the-spot. Thus, spreading can be reduced and safety can be improved. Recently, loop-mediated isothermal-based biosensor and antigen-based biosensing devices are in huge demand compared to any conventional process that takes more than 2 days to obtain the reports. Moreover, integrated biosensor processing with a mobile app has brought a new development in this current situation to track down the infected person easily. Hence, in this Special Issue, our main motivation to highlight new biosensing innovations to the world so that fighting coronaviruses will be easier in the future.

Dr. Sharmili Roy
Dr. Pranjal Chandra
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

biosensors
point-of-care detection
molecular techniques
nanotechnology
integrated biosensensors
isothermal techniques
RNA Biosensor
DNA biosensor
microarray
biomarkers

Published Papers (3 papers)

Download All Papers

Research

Jump to: Review

13 pages, 3963 KiB

Open AccessArticle

Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera

by Ashutosh Kumar, Tamal Sarkar and Pratima R. Solanki

Biosensors 2023, 13(2), 177; https://doi.org/10.3390/bios13020177 - 23 Jan 2023

Cited by 5 | Viewed by 2249

Abstract

Herein, we report the synthesis and functionalization of gadolinium oxide nanoparticles (Gd₂O₃ NPs) to fabricate a highly efficient immunosensor for the detection of Vibrio cholera toxin (CT). Gd₂O₃ NPs were produced in a straightforward manner utilizing the microwave irradiation technique using a domestic microwave oven. X-ray diffraction, transmission electron microscopy, and spectroscopic techniques were used to characterize the structural and physical aspects of Gd₂O₃ NPs. The Gd₂O₃ NPs were then functionalized with 3-(Aminopropyl) triethoxysilane (APTES) and electrophoretically deposited onto an ITO-coated glass substrate. The anti-CT monoclonal antibodies were covalently attached to the APTES-Gd₂O₃/ITO electrode via EDC-NHS chemistry, followed by bovine serum albumin (BSA). For CT detection, electrochemical response experiments using BSA/anti-CT/APTES-Gd₂O₃/ITO immunoelectrodes were carried out (5–700 ng mL⁻¹). The immunoelectrode demonstrated an outstanding electrochemical reaction against CT, with a sensitivity of 8.37 mA ng⁻¹ mL cm⁻² and a detection limit of 1.48 ng mL⁻¹. Full article

(This article belongs to the Special Issue Point-of-Care Detection of COVID-19 and Other Infections Disease: Advancement and Innovations in Biosensing Methods)

► Show Figures

Figure 1

19 pages, 2867 KiB

Open AccessArticle

Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocolitica

by Maria G. Sande, Débora Ferreira, Joana L. Rodrigues, Luís D. R. Melo, Dirk Linke, Carla J. Silva, Felismina T. C. Moreira, Maria Goreti F. Sales and Ligia R. Rodrigues

Biosensors 2022, 12(8), 614; https://doi.org/10.3390/bios12080614 - 8 Aug 2022

Cited by 8 | Viewed by 2922

Abstract

New point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on the cell surface was used as a target cell. After eight cycles of selection, the final aptamer pool was sequenced by high throughput sequencing using the Illumina Novaseq platform. The sequencing data, analyzed using the Geneious software, was aligned, filtered and demultiplexed to obtain the key nucleotides possibly involved in the target binding. The most promising aptamer candidate, Apt1, bound specifically to YadA with a dissociation constant (K_d) of 11 nM. Apt1 was used to develop a simple electrochemical biosensor with a two-step, label-free design towards the detection of YadA. The sensor surface modifications and its ability to bind successfully and stably to YadA were confirmed by cyclic voltammetry, impedance spectroscopy and square wave voltammetry. The biosensor enabled the detection of YadA in a linear range between 7.0 × 10⁴ and 7.0 × 10⁷ CFU mL⁻¹ and showed a square correlation coefficient >0.99. The standard deviation and the limit of detection was ~2.5% and 7.0 × 10⁴ CFU mL⁻¹, respectively. Overall, the results suggest that this novel biosensor incorporating Apt1 can potentially be used as a sensitive POC detection system to aid the diagnosis of Y. enterocolitica infections. Furthermore, this simple yet innovative approach could be replicated to select aptamers for other (bacterial) targets and to develop the corresponding biosensors for their detection. Full article

(This article belongs to the Special Issue Point-of-Care Detection of COVID-19 and Other Infections Disease: Advancement and Innovations in Biosensing Methods)

► Show Figures

Graphical abstract

Review

Jump to: Research

31 pages, 2978 KiB

Open AccessReview

Carbon-Based Fluorescent Nano-Biosensors for the Detection of Cell-Free Circulating MicroRNAs

by Pooja Ratre, Nazim Nazeer, Roshani Kumari, Suresh Thareja, Bulbul Jain, Rajnarayan Tiwari, Arunika Kamthan, Rupesh K. Srivastava and Pradyumna Kumar Mishra

Biosensors 2023, 13(2), 226; https://doi.org/10.3390/bios13020226 - 4 Feb 2023

Cited by 6 | Viewed by 2725

Abstract

Currently, non-communicable diseases (NCDs) have emerged as potential risks for humans due to adopting a sedentary lifestyle and inaccurate diagnoses. The early detection of NCDs using point-of-care technologies significantly decreases the burden and will be poised to transform clinical intervention and healthcare provision. An imbalance in the levels of circulating cell-free microRNAs (ccf-miRNA) has manifested in NCDs, which are passively released into the bloodstream or actively produced from cells, improving the efficacy of disease screening and providing enormous sensing potential. The effective sensing of ccf-miRNA continues to be a significant technical challenge, even though sophisticated equipment is needed to analyze readouts and expression patterns. Nanomaterials have come to light as a potential solution as they provide significant advantages over other widely used diagnostic techniques to measure miRNAs. Particularly, CNDs-based fluorescence nano-biosensors are of great interest. Owing to the excellent fluorescence characteristics of CNDs, developing such sensors for ccf-microRNAs has been much more accessible. Here, we have critically examined recent advancements in fluorescence-based CNDs biosensors, including tools and techniques used for manufacturing these biosensors. Green synthesis methods for scaling up high-quality, fluorescent CNDs from a natural source are discussed. The various surface modifications that help attach biomolecules to CNDs utilizing covalent conjugation techniques for multiple applications, including self-assembly, sensing, and imaging, are analyzed. The current review will be of particular interest to researchers interested in fluorescence-based biosensors, materials chemistry, nanomedicine, and related fields, as we focus on CNDs-based nano-biosensors for ccf-miRNAs detection applications in the medical field. Full article

(This article belongs to the Special Issue Point-of-Care Detection of COVID-19 and Other Infections Disease: Advancement and Innovations in Biosensing Methods)

► Show Figures