Microfluidic Systems for Single Cell Analysis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (31 July 2024) | Viewed by 5636

Special Issue Editors


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Guest Editor
College of Control Science and Engineering, Zhejiang University, Hangzhou 310007, China
Interests: high throughput single cell genomic sequencing; biomicrofluidic chip; digital PCR; deep learning and artificial intelligence
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
College of Control Science and Engineering, Zhejiang University, Hangzhou 310007, China
Interests: microfluidics system and its application in life sciences; life instrumentation; integrated nanobiotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Up to now, microfluidic systems have dramatically facilitated the development of single-cell analysis by leveraging the micro-scale geometry for improving sensitivity, decreasing reaction time, decreasing reagents consumption, and improving parallelization and automation for high throughput. Microfluidic droplets offer extremely high scalability of reactions and straightforward single cell manipulation. Single-cell analysis techniques based on the microfluidics systems have become a powerful tool and a driving force for biological studies and discoveries. This growing field has increased the sensitivity, accuracy, and throughput of traditional single-cell analysis methods. Especially, single-cell sequencing methods based on microfluidics systems have enabled the rapid genome-wide analysis of thousands of single cells. These cutting-edge methods have allowed us to profile cells at unprecedented resolutions and numbers, thus building a complete human cell atlas. Such a comprehensive database would inform us of the fundamental features of each cell type and lead insights into the changes and underlying mechanisms behind biological regulatory mechanisms and evolutionary processes, as well as disease onset and progression. Microfluidic systems also contribute to human tumor atlases, and deciphering this complex issue involves a high degree of heterogeneity among different cell populations and their interactions.

For this Special Issue titled "Microfluidic Systems for Single-Cell Analysis", we welcome original works, perspectives, and reviews including but not limited to the developments and applications of single-cell analysis methods based on microfluidic systems, including single-cell capturing, sorting, culture, imaging, protein analysis, nucleic acid analysis, as well as genomics, transcriptomics, spatial transcriptomics, epigenomics, and multi-omics analyses.

Prof. Dr. Qiangyuan Zhu
Prof. Dr. Ying Mu
Guest Editors

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Keywords

  • single-cell isolation
  • capturing
  • sorting
  • culture
  • imaging
  • protein analysis
  • nucleic acid analysis
  • genomics analysis
  • transcriptomics analysis
  • spatial transcriptomics analysis
  • epigenomics analysis
  • multi-omics analysis

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Published Papers (2 papers)

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Research

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12 pages, 4085 KiB  
Article
D-Glucose-Mediated Gold Nanoparticle Fabrication for Colorimetric Detection of Foodborne Pathogens
by Seo Yeon Park, Rajamanickam Sivakumar and Nae Yoon Lee
Biosensors 2024, 14(6), 284; https://doi.org/10.3390/bios14060284 - 1 Jun 2024
Viewed by 819
Abstract
Gold nanoparticle (AuNP) fabrication via the oxidation of D-glucose is applied for detecting two foodborne pathogens, Enterococcus faecium (E. faecium) and Staphylococcus aureus (S. aureus). D-glucose is used as a reducing agent due to its oxidation to gluconic acid [...] Read more.
Gold nanoparticle (AuNP) fabrication via the oxidation of D-glucose is applied for detecting two foodborne pathogens, Enterococcus faecium (E. faecium) and Staphylococcus aureus (S. aureus). D-glucose is used as a reducing agent due to its oxidation to gluconic acid by sodium hydroxide (NaOH), resulting in the formation of AuNPs. Based on this mechanism, we develop AuNP-based colorimetric detection in conjunction with loop-mediated isothermal amplification (LAMP) for accurately identifying the infectious bacteria. Here, Au+ ions bind to the base of double-stranded DNA. In the presence of D-glucose and NaOH, the LAMP amplicon-Au+ complex maintains its bound state at 65 °C for 10 min while it is reduced to AuNPs in a dispersed form, exhibiting a red color. We aimed to pre-mix D-glucose with LAMP reagents before amplification and induce successful colorimetry without inhibiting amplification to simplify the experimental process and decrease the reaction time. Therefore, the entire process, including LAMP and colorimetric detection, is accomplished in approximately 1 h. The limit of detection of E. faecium and S. aureus is confirmed using the introduced method as 101 CFU/mL and 100 fg/μL, respectively. We expect that colorimetric detection using D-glucose-mediated AuNP synthesis offers an application for simple and immediate molecular diagnosis. Full article
(This article belongs to the Special Issue Microfluidic Systems for Single Cell Analysis)
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Review

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19 pages, 3156 KiB  
Review
Spatial Omics Sequencing Based on Microfluidic Array Chips
by Jianyu Shi, Yating Pan, Xudong Liu, Wenjian Cao, Ying Mu and Qiangyuan Zhu
Biosensors 2023, 13(7), 712; https://doi.org/10.3390/bios13070712 - 6 Jul 2023
Cited by 1 | Viewed by 3828
Abstract
Spatial profiling technologies fill the gap left by the loss of spatial information in traditional single-cell sequencing, showing great application prospects. After just a few years of quick development, spatial profiling technologies have made great progress in resolution and simplicity. This review introduces [...] Read more.
Spatial profiling technologies fill the gap left by the loss of spatial information in traditional single-cell sequencing, showing great application prospects. After just a few years of quick development, spatial profiling technologies have made great progress in resolution and simplicity. This review introduces the development of spatial omics sequencing based on microfluidic array chips and describes barcoding strategies using various microfluidic designs with simplicity and efficiency. At the same time, the pros and cons of each strategy are compared. Moreover, commercialized solutions for spatial profiling are also introduced. In the end, the future perspective of spatial omics sequencing and research directions are discussed. Full article
(This article belongs to the Special Issue Microfluidic Systems for Single Cell Analysis)
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