Nanosensors for the Detection and Characterization of Food, Environmental and Medical Samples—2nd Edition

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 467

Special Issue Editor

Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA, USA
Interests: biosensors; nanotechnology; spectroscopy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The development of rapid-response biosensing technologies utilizing nanotechnology has become more relevant for furthering our understanding of biological phenomena related to food, agriculture, environment, and medicine. As we understand the molecular mechanisms that underlie the emergence and spread of pathogens/toxins and their consequent impact on our agricultural and food systems, environment and ecosystems, as well as public health, and gain insight into the molecular mechanisms of life itself, it becomes clear that the technologies to investigate, intervene, and mitigate need to be correspondingly small (i.e., within the realm of nanotechnology).

In this Special Issue, we will focus on research furthering our understanding of the interaction between nanoscale phenomena and various reagents, with a view to developing better nano-based biosensing technology. We will report novel devices and applications using nano-based approaches that can detect various pathogens and/or toxins and chemical pollutants faster and more accurately—especially in the areas of food, environmental and medical samples. This Special Issue will also discuss specificity and sensitivity challenges that still face biosensors applications, and hurdles to be overcome for more successful commercialization in various biosensor technologies. We would like to invite all researchers interested in these topics to join the discussion, and to show their work and findings to the scientific community to advance the welfare of human society. In light of the ongoing COVID-19 pandemic, progress in biosensor technologies is more important than ever before. We look forward to your contributions.

Dr. Chenxu Yu
Guest Editor

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Keywords

  • biosensors
  • environmental
  • surveillance
  • pathogens
  • foods
  • medical
  • diagnosis
  • nanomaterials
  • optical
  • electrochemical
  • signal
  • processing
  • nanoimpact

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Published Papers (1 paper)

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Research

14 pages, 1793 KiB  
Article
A Metal–Organic Hybrid Composed of Dual Quenching Cofactors as a Nanoquencher for the Fluorescent Determination of Protease Caspase-3
by Fengli Gao, Lin Liu, Cancan He, Yong Chang and Weiqiang Wang
Biosensors 2025, 15(6), 354; https://doi.org/10.3390/bios15060354 - 4 Jun 2025
Viewed by 403
Abstract
Nanoquenchers with a single quenching cofactor exhibit limited fluorescence quenching efficiency. In this work, a metal–organic hybrid with dual quenching cofactors (Cu2+ and pyrroloquinoline quinone or PQQ) was prepared by metal-coordinated assembly and used as a nanoquencher for a protease assay with [...] Read more.
Nanoquenchers with a single quenching cofactor exhibit limited fluorescence quenching efficiency. In this work, a metal–organic hybrid with dual quenching cofactors (Cu2+ and pyrroloquinoline quinone or PQQ) was prepared by metal-coordinated assembly and used as a nanoquencher for a protease assay with enhanced quenching efficiency. The peptide substrate with an oligohistidine (His6) tag was labeled with a fluorophore. Caspase-3 was determined as a protease example. The substrate was attached onto the surface of the Cu-PQQ nanoquencher by a metal coordination interaction between the unsaturated Cu2+ on the nanoparticle surface and the His6 tag in the peptide. The cleavage of the peptide substrate by enzymatic hydrolysis led to the release of a fluorophore-conjugated segment from the nanoquencher surface, thus turning on the fluorescence. The nanoprobe was used to determine caspase-3 with a linear range of 0.01–5 ng/mL and a detection limit of 7 pg/mL. Furthermore, the method was used to evaluate inhibition efficiency and monitor drug-induced cell apoptosis. In contrast to other means of peptide immobilization, such as physical adsorption and covalent coupling, the strategy based on the metal coordination interaction is simple and powerful, thereby achieving assays of caspase-3 activity in lysates with a satisfactory result. The work should be valuable for the design of nanoquenchers with multiple quenching cofactors and the development of novel biosensors. Full article
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