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Feature Papers on Developmental and Reproductive Biology
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Feature Papers on Developmental and Reproductive Biology

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Developmental and Reproductive Biology".

Deadline for manuscript submissions: 15 September 2025 | Viewed by 13219

Special Issue Editor

Prof. Dr. Jacek Z. Kubiak

E-Mail Website
Guest Editor
Dynamics and Mechanics of Epithelia Group, Faculty of Medicine, Institute of Genetics and Development of Rennes, University of Rennes, CNRS, UMR 6290, 35043 Rennes, France
Interests: embryo development; cell cycle; gene regulation; cancer; stem cells; gonads; genetic diseases
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue, “Feature Papers on Developmental and Reproductive Biology”, aims to publish original and review articles focusing on mechanisms of embryonic development; cell differentiation; growth; homeostasis; regeneration in humans, animals, plants, and unicellular organisms; stem cells; organoids; sex differentiation; gonad development; the hormonal regulation of reproduction; reproductive immunology and toxicology; and environmental effects on health, reproduction, and embryo development. 

We also invite manuscripts covering the immunological aspects of pregnancy and the mechanisms of immune tolerance of genetically disparate embryos by the mother. In this Issue, we wish to focus on the molecular, cellular, genetic, and evolutionary aspects of developmental and reproductive processes in all organisms. We are also interested in publishing papers on aging and pathologies related to reproduction and developmental processes, such as cancers, genetic disorders, or toxicologic adverse effects. 

We welcome your submissions to this Special Issue of Biology on any of the aforementioned subject areas and any others that are closely related to all aspects of developmental and reproductive biology.

Prof. Dr. Jacek Z. Kubiak
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • embryo/fetus development
  • reproductive biology
  • gametogenesis
  • differentiation
  • growth
  • homeostasis
  • regeneration
  • aging
  • cell cycle regulation
  • stem cells
  • organoids

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Published Papers (6 papers)

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Research

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18 pages, 38533 KiB  
Article
Integration of Microarray and Single-Cell RNA-Seq Data and Machine Learning Allows the Identification of Key Histone Modification Gene Changes in Spermatogonial Stem Cells
by Ali Shakeri Abroudi, Hossein Azizi, Melika Djamali, Ali Qorbanee and Thomas Skutella
Biology 2025, 14(4), 387; https://doi.org/10.3390/biology14040387 - 8 Apr 2025
Viewed by 991
Abstract
Histone modifications play a critical role in regulating gene expression and maintaining the functionality of spermatogonial stem cells (SSCs), which are essential for male fertility and spermatogenesis. In this study, we integrated microarray and single-cell RNA-sequencing (scRNA-seq) data to identify key histone modification [...] Read more.
Histone modifications play a critical role in regulating gene expression and maintaining the functionality of spermatogonial stem cells (SSCs), which are essential for male fertility and spermatogenesis. In this study, we integrated microarray and single-cell RNA-sequencing (scRNA-seq) data to identify key histone modification gene changes associated with SSC function and aging. Through differential expression analysis, we identified 2509 differentially expressed genes (DEGs) in SSCs compared to fibroblasts. Among these, genes involved in histone modification, such as KDM5B, SCML2, SIN3A, and ASXL3, were highlighted for their significant roles in chromatin remodeling and gene regulation. Protein–protein interaction (PPI) networks and gene ontology (GO) enrichment analysis revealed critical biological processes such as chromatin organization, histone demethylation, and chromosome structure maintenance. Weighted gene co-expression network analysis (WGCNA) further revealed three key modules of co-expressed genes related to spermatogonial aging. Additionally, ligand–receptor interaction scoring based on tumor microenvironment analysis suggested potential signaling pathways that could influence the stemness and differentiation of SSCs. Our findings provide new insights into the molecular mechanisms underlying SSC aging, highlighting histone modification genes as potential therapeutic targets for preserving male fertility and improving SSC-culturing techniques. This study advances our understanding of histone modification in SSC biology and will serve as a valuable resource for future investigations into male fertility preservation. Full article
(This article belongs to the Special Issue Feature Papers on Developmental and Reproductive Biology)
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17 pages, 1355 KiB  
Article
Impact of Kalanchoe (Kalanchoe daigremontiana) Supplementation in Goat Maternal Diet on Hepatic and Renal Function and Reproductive Performance
by Juan M. Vázquez-García, Gilberto Ballesteros-Rodea, Venancio Cuevas-Reyes, Luisa E. S. Hernández-Arteaga, Luz Y. Peña-Avelino, Samuel López-Aguirre, Reagan Sims, Jaime M. Cavazos-Galindo and Cesar A. Rosales-Nieto
Biology 2025, 14(4), 376; https://doi.org/10.3390/biology14040376 - 5 Apr 2025
Viewed by 645
Abstract
Kalanchoe daigremontiana, a medicinal plant rich in bioactive compounds, has the potential to serve as a feed supplement, reducing reliance on conventional livestock medications while potentially enhancing productivity. This study evaluated the effects of K. daigremontiana supplementation over 52 days on kidney [...] Read more.
Kalanchoe daigremontiana, a medicinal plant rich in bioactive compounds, has the potential to serve as a feed supplement, reducing reliance on conventional livestock medications while potentially enhancing productivity. This study evaluated the effects of K. daigremontiana supplementation over 52 days on kidney and liver function, metabolism, weight changes, and reproductive efficiency in multiparous Alpine goats during the breeding season. Fifty-five goats were assigned to either a control (CTL, n = 27; 47.6 ± 1.1 kg) or a K. daigremontiana-supplemented (KAL, n = 28; 47.6 ± 1.3 kg) diet. The KAL group received K. daigremontiana at a dry matter-based inclusion rate of 2 kg t−1 of feed, while the CTL group received no supplementation. Blood samples were collected at four time points to assess the biochemical markers of kidney and liver function. Pregnancy was achieved through natural mating, and reproductive efficiency was evaluated. Overall, liver and kidney function did not differ significantly between treatments (p > 0.05). However, KAL supplementation was associated with increased creatinine (p < 0.05), ALP (p < 0.001), and bilirubin (p < 0.05) at specific time points, whereas CTL goats exhibited higher BUN (p < 0.001), AST (p < 0.05), albumin (p < 0.001), total protein (p ≤ 0.05), and phosphorus (p < 0.01) on specific dates. Other metabolic markers, weight gain, and reproductive efficiency did not differ between treatments (p > 0.05). Overall, Kalanchoe daigremontiana supplementation had mild, transient effects on goat health without significantly impacting productivity. Further research is needed to explore its long-term effects and optimal dosage for livestock nutrition. Full article
(This article belongs to the Special Issue Feature Papers on Developmental and Reproductive Biology)
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20 pages, 833 KiB  
Article
Twinning and Individuation: An Appraisal of the Current Model and Ethical Implications
by Francis J. O’Keeffe and George L. Mendz
Biology 2025, 14(2), 104; https://doi.org/10.3390/biology14020104 - 21 Jan 2025
Viewed by 5452
Abstract
Discourses on human embryo experimentation often refer to monozygotic twinning and individuation. A criterion to establish regulations that guide human embryo research proposes that individuation is achieved once the embryo ceases to have the potential for dividing into two or more viable entities [...] Read more.
Discourses on human embryo experimentation often refer to monozygotic twinning and individuation. A criterion to establish regulations that guide human embryo research proposes that individuation is achieved once the embryo ceases to have the potential for dividing into two or more viable entities at about 15 days of gestational age. This standard is based on an updated version of a developmental model initially proposed by George Corner. A fundamental problem with this approach is the model’s lack of sufficient evidence to explain adequately human embryo twinning and, consequently, to serve as a basis to establish appropriate ethical guidelines for embryo experimentation. In addition, subsequent formulations of Corner’s model added an extension of blastomere totipotency to different moments of gestation, without a proper scientific basis. The model is also challenged by monozygotic twinnings that result in placental and amniotic arrangements incompatible with Corner’s framework. Investigations into the physiology of fertilisation and of the zygote suggest that individuation may occur at a very early stage. An alternative description of monozygotic twinning may explain better sesquizygotic twinning events and serve to re-evaluate the individuation criterion. The study aims to investigate deficiencies in the embryology of this model and assess their ethical implications. Full article
(This article belongs to the Special Issue Feature Papers on Developmental and Reproductive Biology)
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14 pages, 6257 KiB  
Article
Impact of Benzodiazepine Delorazepam on Growth and Behaviour of Artemia salina Nauplii
by Chiara Fogliano, Rosa Carotenuto, Claudio Agnisola, Chiara Maria Motta and Bice Avallone
Biology 2024, 13(10), 808; https://doi.org/10.3390/biology13100808 - 10 Oct 2024
Cited by 1 | Viewed by 1751
Abstract
Benzodiazepines, a significant group of newly recognised water contaminants, are psychotropic medications prescribed for common anxiety symptoms and sleep disorders. They resist efficient degradation during sewage treatment and endure in aquatic environments. Their presence in aquatic matrices is increasing, particularly after the recent [...] Read more.
Benzodiazepines, a significant group of newly recognised water contaminants, are psychotropic medications prescribed for common anxiety symptoms and sleep disorders. They resist efficient degradation during sewage treatment and endure in aquatic environments. Their presence in aquatic matrices is increasing, particularly after the recent pandemic period, which has led many people to systematically use benzodiazepines to manage anxiety. In previous studies, an important interference of this class of drugs on both the larval and adult stages of some aquatic species has been demonstrated, with effects on behaviour and embryonic development. This study examined the influence of delorazepam, a diazepam metabolite, on Artemia salina development to gain insight into responses in naupliar larvae. Results demonstrated that treatments (1, 5, and 10 µg/L) increase the hatching percentage and induce a desynchronisation in growth. Mortality was only slightly increased (close to 10% at six days post-hatching), but lipid reserve consumption was modified, with the persistence of lipid globules at the advanced naupliar stages. Locomotory activity significantly decreased only at 10 µg/L treatment. No teratogenic effects were observed, though modest damages were noticed in the posterior trunk and eyes, two targets of environmental toxicity. The negative impact of delorazepam on Artemia salina adds to those already reported in other species of invertebrates and vertebrates, which are not yet considered targets of these drugs. This study underscores the need for further research and immediate attention to this class of contaminants and the importance of monitoring their presence during environmental risk assessments. Full article
(This article belongs to the Special Issue Feature Papers on Developmental and Reproductive Biology)
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Review

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21 pages, 1761 KiB  
Review
Unlocking Gamete Quality Through Extracellular Vesicles: Emerging Perspectives
by Notsile H. Dlamini, Alessandra Bridi, Juliano Coelho da Silveira and Jean M. Feugang
Biology 2025, 14(2), 198; https://doi.org/10.3390/biology14020198 - 13 Feb 2025
Viewed by 1318
Abstract
Extracellular vesicles (EVs) are gaining recognition for their essential role in enhancing gamete quality and improving outcomes in assisted reproductive technologies. These nanosized particles, released by cells, carry proteins, lipids, and RNAs, facilitating critical cell communication and offering the potential to enhance gamete [...] Read more.
Extracellular vesicles (EVs) are gaining recognition for their essential role in enhancing gamete quality and improving outcomes in assisted reproductive technologies. These nanosized particles, released by cells, carry proteins, lipids, and RNAs, facilitating critical cell communication and offering the potential to enhance gamete maturation and improve fertilization rates. Most research on males has concentrated on seminal plasma, a complex fluid produced by the testes and accessory glands vital in modulating sperm fertility potential. The components of seminal plasma significantly affect sperm functionality, embryo survival, and placental development, making this a prominent area of interest in reproductive biology. The EVs within seminal plasma contribute to maintaining sperm membrane stability, enhancing motility, and promoting capacitation, which may influence the female reproductive tract following mating. In females, EVs have been identified in both the follicular and uterine environments, where effective embryo–maternal communication is crucial. The oviduct epithelium supports gamete transport and early embryonic development, with EVs found in oviductal fluid playing a key role in reproductive processes. These EVs support the embryo’s growth in the nutrient-rich uterine environment. These important studies underscore the significant role of EVs in transporting essential molecular compounds to gametes and embryos, leading to an enhanced understanding and potential manipulation of reproductive processes. This review aims to summarize the current research on the benefits of EVs in gamete manipulation and embryo development, highlighting their promising implications for reproductive health. Full article
(This article belongs to the Special Issue Feature Papers on Developmental and Reproductive Biology)
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11 pages, 601 KiB  
Review
Evolution of Media Supporting the Development of Mammalian Preimplantation Embryos In Vitro
by Hongsheng Men
Biology 2024, 13(10), 789; https://doi.org/10.3390/biology13100789 - 1 Oct 2024
Cited by 2 | Viewed by 1776
Abstract
Assisted reproductive technology has revolutionized our ability to genetically manipulate, maintain and rederive laboratory animals of biomedical importance; manipulate animal reproduction or genetics to boost production of farm animals; and improve human reproductive health. The media for in vitro manipulation and the culture [...] Read more.
Assisted reproductive technology has revolutionized our ability to genetically manipulate, maintain and rederive laboratory animals of biomedical importance; manipulate animal reproduction or genetics to boost production of farm animals; and improve human reproductive health. The media for in vitro manipulation and the culture of embryos play a critical role in the development of assisted reproductive technology. In this review, the evolution of culture media supporting embryo development in vitro from selected animal species, laboratory animals (mice and rats) and farm animals (pigs and cattle), will be discussed with a focus on the development of chemically defined media. Full article
(This article belongs to the Special Issue Feature Papers on Developmental and Reproductive Biology)
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