Antioxidants Isolation and Characterization

A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "Methods for Antioxidants Evaluation/Measurement".

Deadline for manuscript submissions: 31 December 2026 | Viewed by 1216

Special Issue Editors


E-Mail Website
Guest Editor
Department of Analytical Chemistry, Nutrition and Food Science, University of Alicante, 03690 San Vicente del Raspeig, Spain
Interests: development of analytical methods for food, clinical, environmental, and (bio)fuel samples; advances in chromatography and atomic spectroscopy; analysis of microsamples; study of interferences in ICP-OES and ICP-MS techniques; chromatograph–ICP coupling for detailed chemical information; application of laser ablation for the introduction of solid samples; development of novel liquid sample introduction systems; design of innovative extraction methods for bioactive products; elemental speciation studies
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Department of Analytical Chemistry, Nutrition and Food Science, University of Alicante, 03690 Alicante, Spain
Interests: active food packaging; polymers characterization; analytical chemistry; food chemistry; food authentication; food waste reduction; polyphenols; volatile composition; antioxidant activity; oxidative stability of foods; environmental analysis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Antioxidants from plant sources constitute a wide and heterogeneous group of secondary metabolites, including polyphenols, flavonoids, tannins, phenolic acids, carotenoids, and tocopherols. These compounds have attracted growing interest due to their capacity to counteract oxidative damage associated with chronic diseases such as cardiovascular disorders, diabetes, and neurodegenerative conditions. Beyond their relevance in human health, plant-derived antioxidants are also of considerable importance in food technology, where they contribute to the stabilization of products, and in the development of nutraceuticals, pharmaceuticals, and cosmetic formulations.

Recent years have witnessed significant progress in both extraction and characterization strategies. Innovative techniques—such as ultrasound- and microwave-assisted extraction, supercritical fluid extraction, and the use of natural deep eutectic solvents—are providing more efficient, selective, and environmentally sustainable alternatives to conventional methods. In parallel, advances in chromatographic, spectroscopic, and metabolomic platforms allow deeper insights into the chemical diversity and structural complexity of plant antioxidants, enabling their accurate identification and quantification even within complex matrices.

This Special Issue of Antioxidants would bring together contributions that address fundamental and applied aspects of plant-derived antioxidants. Topics of interest include, but are not limited to: the optimization of novel extraction processes; chemical and structural elucidation of antioxidant compounds; evaluation of antioxidant capacity and mechanisms of action in vitro, in vivo, and in silico; and the valorization of agricultural by-products as cost-effective sources of bioactive molecules.

By providing a comprehensive overview of recent advances, this Special Issue aims to enhance understanding of the chemistry, functionality, and applications of natural antioxidants, while fostering interdisciplinary collaborations across food science, chemistry, biotechnology, and health-related disciplines.

Prof. Dr. José Luis Todolí Torró
Prof. Dr. Ana Beltrán Sanahuja
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antioxidants is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant-derived antioxidants
  • polyphenols
  • flavonoids
  • carotenoids
  • phenolic acids
  • green extraction techniques
  • chromatographic and spectroscopic analysis
  • metabolomics
  • antioxidant activity evaluation
  • agro-industrial by-products valorization

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

15 pages, 1526 KB  
Article
Absolute Quantitation of Phenolic Compounds in Olive Oil for Health Claim Recognition
by Ana Castillo-Luna and Feliciano Priego-Capote
Antioxidants 2026, 15(4), 511; https://doi.org/10.3390/antiox15040511 - 20 Apr 2026
Cited by 1 | Viewed by 377
Abstract
The European Regulation (UE) 432/2012 includes a specific health claim for olive-oil-associated with its phenolic content, which is based on its protective role against lipid oxidation in the blood. To make use of the health claim, olive oil must have a minimum concentration [...] Read more.
The European Regulation (UE) 432/2012 includes a specific health claim for olive-oil-associated with its phenolic content, which is based on its protective role against lipid oxidation in the blood. To make use of the health claim, olive oil must have a minimum concentration in phenolic compounds of 250 mg/kg. Reviewing the health claim, the phenolic compounds referred to are the secoiridoid derivatives of hydroxytyrosol and tyrosol. A method based on absolute quantification of phenolic compounds in olive oil is proposed for the recognition of the health claim. The method involves liquid–liquid extraction with a 1:8 (v/v) oil:extract ratio to avoid saturation of the extract in oils with a higher phenolic content and its subsequent determination through LC–MS/MS in multiple reaction monitoring (MRM) mode, the gold standard technique in many application fields because of its analytical features. The optimized method was applied to a set of 100 extra virgin olive oils (EVOOs), and the results obtained were compared with the classic Folin–Ciocalteu method. The comparison between the two methods showed that the classic method is a non-selective method that can be affected by many interferences and that the Folin method underestimates the real phenolic content. Full article
(This article belongs to the Special Issue Antioxidants Isolation and Characterization)
Show Figures

Graphical abstract

14 pages, 889 KB  
Article
Trace Element Imbalance and Redox-Relevant Serum Profile in Hemodialysis: A Validated Multielement ICP-MS Study
by Sara Valderrama-Sanz, Jordi Tortosa-Carreres, Ricardo Molina-Gasset and José-Luis Todolí-Torró
Antioxidants 2026, 15(4), 457; https://doi.org/10.3390/antiox15040457 - 8 Apr 2026
Viewed by 496
Abstract
Chronic kidney disease (CKD) is associated with persistent inflammation and increased oxidative stress. Trace elements play an important role in these processes as modulators of redox balance, acting either as cofactors of antioxidant systems or as potential inducers of pro-oxidant mechanisms. In this [...] Read more.
Chronic kidney disease (CKD) is associated with persistent inflammation and increased oxidative stress. Trace elements play an important role in these processes as modulators of redox balance, acting either as cofactors of antioxidant systems or as potential inducers of pro-oxidant mechanisms. In this study, the serum profile of trace elements with antioxidant and pro-oxidant relevance was characterized by inductively coupled plasma mass spectrometry (ICP-MS) in hemodialysis patients. Results were compared with those from a healthy control group, and associations with biochemical and hematological parameters were explored. A cross-sectional observational study was conducted including 117 hemodialysis patients and 82 healthy controls, determining a panel of eight trace elements (Zn, Se, Cu, Mn, As, Cr, Co, and Ni). The method showed adequate sensitivity, satisfactory precision for most elements, and acceptable trueness. Hemodialysis patients exhibited significantly lower serum concentrations of Zn and Se, together with greater interindividual variability, as well as higher concentrations of elements with potential pro-oxidant effects, including As, Cr, Co, and Ni. Within this group, Zn and Se were mainly associated with markers of the protein compartment, Cu with ceruloplasmin and inflammatory markers, and Cr and Ni with parameters of renal function and vitamin D status. Full article
(This article belongs to the Special Issue Antioxidants Isolation and Characterization)
Show Figures

Figure 1

Back to TopTop