Oxidative Stress in Animal Reproduction and Nutrition

A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "Health Outcomes of Antioxidants and Oxidative Stress".

Deadline for manuscript submissions: 28 November 2025 | Viewed by 2743

Special Issue Editors

Guangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Interests: sow nutrition; placenta; intrauterine growth restriction; nutrient transport
Special Issues, Collections and Topics in MDPI journals
Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
Interests: sow and piglet nutrition; antioxidants; oxidative stress; trace elements; plant-derived bioactive additives
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Oxidative stress plays an important role in animals during reproductive phases. In turn, reproductive phases are commonly regarded as a significant source of oxidative stress. Furthermore, a myriad of stress challenges may be encountered, resulting in oxidative stress for reproductive animals, including environmental stress (such as heat stress), dietary stress sources (such as exposure to mycotoxins and the utilization of oxidized oil or fat in the diets), and social stress, among others.

This research topic will mainly focus on identifying the potential sources of oxidative stress in animal reproduction, as well as investigating the ameliorative effects of nutritional interventions on oxidative injury, thereby resulting in improved productivity. To begin with, the present topic will concentrate on stress sources that have the potential to lead to oxidative stress, which may occur in animals during specific physiological phases, such as gestation and lactation in female mammals and service male animals, as well as laying poultry. It may also be induced by various forms of environmental stress, including heat stress, mycotoxin exposure, and management stress (social stress).

Additionally, further emphasis will be placed on the nutritional regulation of antioxidant function and oxidative stress status in reproductive animals, utilizing either animals or in vitro cells. The nutrients include, but are not limited to, vitamins, trace elements, amino acids, fatty acids, and plant-derived polyphenols. In addition, this topic encourages research involving model animals, such as mice and rats, to investigate the underlying mechanisms of oxidative stress or antioxidant defences in female and male reproduction.

Dr. Fang Chen
Dr. Jun Chen
Guest Editors

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Keywords

  • oxidative stress
  • antioxidants
  • animal reproduction
  • nutritional regulation
  • livestock and poultry
  • mouse or rat model
  • antioxidant mechanisms
  • oxidative stress mechanisms

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Published Papers (3 papers)

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Research

27 pages, 11269 KB  
Article
Integrated Transcriptomic and Metabolomic Analysis Reveals Regulatory Effects of Fermented Chinese Chive on Early Testicular Development in Piglets
by Yupeng Xie, Suthar Teerath Kumar, Hong Zou, Ting-Ting Luo, Yunpeng Zhang, Qi Zhang, Yang Li, Kai-Min Niu, Zhenya Zhai, Chunfeng Wang, Wu-Sheng Sun and Shu-Min Zhang
Antioxidants 2025, 14(9), 1056; https://doi.org/10.3390/antiox14091056 - 28 Aug 2025
Viewed by 560
Abstract
Early testicular development is vital for adult male fertility but remains highly vulnerable to stress during the suckling stage. Fermented Chinese chive (Allium tuberosum) is known for its antioxidant and immunomodulatory properties, yet its role in testicular development remains unclear. In [...] Read more.
Early testicular development is vital for adult male fertility but remains highly vulnerable to stress during the suckling stage. Fermented Chinese chive (Allium tuberosum) is known for its antioxidant and immunomodulatory properties, yet its role in testicular development remains unclear. In this study, Songliao Black piglets received 3‰ fermented Chinese chive (LK group) mixed with starter feed and compared to a control (OD group). Testicular samples at weaning (28 days) underwent transcriptomic and metabolomic analyses. Although no significant differences were observed in gross testicular morphology, the LK group significantly increased individual (13.85%) and litter (15.11%) weaning weights (p < 0.05), with elevated serum triglycerides, total cholesterol, and a 32.2% rise in IgG levels (p < 0.05). Integrated analysis identified 76 shared pathways, including ferroptosis, insulin resistance, PI3K-Akt, MAPK, and cAMP signaling. Upregulated genes in the LK group were mainly related to energy metabolism, antioxidant defense, immune regulation, steroidogenesis, and neuroendocrine signaling, suggesting improved metabolic activity, reduced oxidative stress, and accelerated reproductive maturation. Molecular docking indicated that kaempferol and isorhamnetin from Chinese chive bind strongly to proteins involved in testicular development. Overall, fermented Chinese chive supplementation enhances early testicular development in suckling piglets via integrated modulation of metabolic, immune, and signaling pathways, providing a nutritional strategy to optimize reproductive potential in breeding boars. Full article
(This article belongs to the Special Issue Oxidative Stress in Animal Reproduction and Nutrition)
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22 pages, 7389 KB  
Article
Mangosteen Pericarp Extract Mitigates Diquat-Induced Hepatic Oxidative Stress by NRF2/HO-1 Activation, Intestinal Barrier Integrity Restoration, and Gut Microbiota Modulation
by Weichen Huang, Yujie Lv, Chenhao Zou, Chaoyue Ge, Shenao Zhan, Xinyu Shen, Lianchi Wu, Xiaoxu Wang, Hongmeng Yuan, Gang Lin, Dongyou Yu and Bing Liu
Antioxidants 2025, 14(9), 1045; https://doi.org/10.3390/antiox14091045 - 25 Aug 2025
Viewed by 728
Abstract
Poultry production exposes birds to diverse environmental and physiological stressors that disrupt redox balance, impair gut–liver axis function, and undermine health and productivity. This study investigated the hepatoprotective and antioxidative effects of mangosteen pericarp extract (MPE) in an experimental model of diquat-induced oxidative [...] Read more.
Poultry production exposes birds to diverse environmental and physiological stressors that disrupt redox balance, impair gut–liver axis function, and undermine health and productivity. This study investigated the hepatoprotective and antioxidative effects of mangosteen pericarp extract (MPE) in an experimental model of diquat-induced oxidative stress in laying hens. A total of 270 Hy-Line White laying hens were randomly assigned to three groups: control group (CON), diquat-challenged group (DQ), and MEP intervention with diquat-challenged group (MQ), with six replicates of 15 birds each. The results showed that MPE supplementation effectively mitigated the hepatic oxidative damage caused by diquat, as evidenced by the increased ALT and AST activity, improved lipid metabolism, and reduced hepatic fibrosis. Mechanistically, MPE activated the NRF2/HO-1 antioxidant pathway, thus enhancing the liver’s ability to counteract ROS-induced damage and reducing lipid droplet accumulation in liver tissue. MPE supplementation restored intestinal barrier integrity by upregulating tight junction protein expression (Occludin-1 and ZO-1), enhancing MUC-2 expression, and thereby decreasing gut microbiota-derived LPS transferring from the intestine. Additionally, MPE also modulated gut microbiota composition by enriching beneficial bacterial genera such as Lactobacillus and Ruminococcus while suppressing the growth of potentially harmful taxa (e.g., Bacteroidales and UCG-010). Fecal microbiota transplantation (FMT) from MPE-treated donors into diquat-exposed recipients reproduced these beneficial effects, further highlighting the role of gut microbiota modulation in mediating MPE’s systemic protective actions. Together, these findings demonstrated that MPE alleviated DQ-induced liver injury and oxidative stress through a combination of antioxidant activity, protection of intestinal barrier function, and modulation of gut microbiota, positioning MPE as a promising natural strategy for mitigating oxidative stress-related liver damage by regulating the gut microbiota and gut–liver axis in poultry. Full article
(This article belongs to the Special Issue Oxidative Stress in Animal Reproduction and Nutrition)
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21 pages, 3896 KB  
Article
Dietary Glyceryl Monolaurate Supplementation During Pregnancy Enhances Fetal Intrauterine Development and Antioxidant Capacity in Sows via Microbiota Modulation
by Zhichao Fu, Jun Wang, Yueqi Zhao, Tanyi Deng, Ziwei Ma, Wutai Guan, Xiangfang Zeng and Fang Chen
Antioxidants 2025, 14(7), 783; https://doi.org/10.3390/antiox14070783 - 25 Jun 2025
Viewed by 583
Abstract
This study elucidates the mechanisms underlying the positive effect of glyceryl monolaurate (GML) on fetal intrauterine development via maternal gut-microbiota modulating effects using a sow model. Addition of GML (1000 mg/kg) improved neonatal intestinal conditions (jejunal villus height, VH/CD ratio and tight junctions) [...] Read more.
This study elucidates the mechanisms underlying the positive effect of glyceryl monolaurate (GML) on fetal intrauterine development via maternal gut-microbiota modulating effects using a sow model. Addition of GML (1000 mg/kg) improved neonatal intestinal conditions (jejunal villus height, VH/CD ratio and tight junctions) and dorsal longissimus muscle (MyoD, MyoG and MSTN) development in the GML-treated group. Furthermore, GML improved maternal gut microbiota composition by enriching short-chain fatty acid (SCFA)-producing bacteria Lactobacillus and Akkermansia. Meanwhile, SCFA concentrations in sow feces and newborn plasma, as well as their receptors (GPR41/43) in intestine and muscle were upregulated with GML, corresponding with enhanced antioxidative and anti-inflammatory capacity. Further correlation analysis revealed Akkermansia and Lactobacillus positively correlated with SCFAs, antioxidative indicators, and anti-inflammatory capacity markers. Moreover, GML inhibited the activation of the MAPK/NF-κB inflammatory signaling pathway. In summary, GML enhanced fetal intrauterine development by modulating sow intestinal SCFA-producing bacteria. Full article
(This article belongs to the Special Issue Oxidative Stress in Animal Reproduction and Nutrition)
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