Animals

Article

38 pages, 8260 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Understanding the Influence of Early-Life Stressors on Social Interaction, Telomere Length, and Hair Cortisol Concentration in Homeless Kittens

by Jennifer Vernick, Chelsea Martin, William Montelpare, Arthur E. Dunham and Karen L. Overall

Animals 2025, 15(3), 446; https://doi.org/10.3390/ani15030446 - 6 Feb 2025

Viewed by 1498

Abstract

The early postnatal period is a critical neurodevelopmental stage characterized by rapid neural maturation and is adversely affected by early-life stressors. This study explored the behavioural, physiological, and epigenetic consequences of early-life stress in a population of homeless rescue kittens. This longitudinal study [...] Read more.

The early postnatal period is a critical neurodevelopmental stage characterized by rapid neural maturation and is adversely affected by early-life stressors. This study explored the behavioural, physiological, and epigenetic consequences of early-life stress in a population of homeless rescue kittens. This longitudinal study included 50 kittens rescued and placed into foster care by the Prince Edward Island Humane Society. They underwent behavioural testing at 8, 10, and 12 weeks of age. Hair cortisol concentration was measured at 8 weeks and served as a physiological marker of the previous 3 months’ cumulative stress response, which, for these kittens, included the late gestation period. A blood sample for relative telomere length measurement was taken at 10–12 weeks to estimate epigenetic changes as young kittens. Data were analyzed with respect to age and performance in all repeated measures tests, status as a stray or a surrender, and the presence of the dam in their foster homes. As expected, the performance of kittens in all tests changed over the 5 weeks of testing. Kittens separated from their mothers exhibited significantly higher hair cortisol concentrations (p = 0.02) and elongated relative telomere lengths (p = 0.04). No correlation was found between hair cortisol concentration and relative telomere lengths (p = 0.99). These results support the need for further study on the effects of epigenetics and early-life stress, both in kittens and across species. Full article

(This article belongs to the Section Animal Physiology)

► Show Figures

Figure 1

11 pages, 1327 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Effects of a Cannabinoid-Based Phytocomplex (Pain Relief^TM) on Chronic Pain in Osteoarthritic Dogs

by Ruggero Amato, Eleonora Pacifico, Daria Lotito, Valeria Iervolino, Ludovica Pierantoni, Laura Cortese, Nadia Musco, Pietro Lombardi, Vincenzo Mastellone and Maria Elena Pero

Animals 2025, 15(1), 101; https://doi.org/10.3390/ani15010101 - 5 Jan 2025

Viewed by 4678

Abstract

Twenty-one adult crossbreed dogs with chronic pain due to severe osteoarthrosis were enrolled in the study (placebo vs. treatment groups). The dogs in the experimental group received the dietary supplement (Pain Relief^TM, Giantec, Isernia, Italy) for 30 days to evaluate its [...] Read more.

Twenty-one adult crossbreed dogs with chronic pain due to severe osteoarthrosis were enrolled in the study (placebo vs. treatment groups). The dogs in the experimental group received the dietary supplement (Pain Relief^TM, Giantec, Isernia, Italy) for 30 days to evaluate its effects on metabolism and pain relief. During the trial, the Helsinki Chronic Pain Index significantly decreased (p < 0.01) in the experimental group, indicating reduced pain and improved quality of life. Additionally, the treated group showed improvements in oxidative stress, demonstrated by a reduction in reactive oxygen metabolites, and an increase in biological antioxidant potential. Interleukins 6 levels decreased in the treated group, while interleukins 10 levels increased, thus suggesting an anti-inflammatory effect of the supplement. Importantly, no adverse effects were observed. Results suggest that Pain Relief^TM is effective in ameliorating osteoarthritis in dogs, improving their quality of life. Full article

(This article belongs to the Collection Nutraceuticals and Animal Physiology: Performance and Welfare)

► Show Figures

Figure 1

15 pages, 1767 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Using Social Network Analysis to Assess ‘Groupness’ in a Mixed-Species Zoo Exhibit of Tufted Capuchins (Sapajus apella) and Squirrel Monkeys (Saimiri sciureus)

by Sophia Daoudi-Simison, Phyllis Lee and Hannah M. Buchanan-Smith

Animals 2024, 14(23), 3360; https://doi.org/10.3390/ani14233360 - 22 Nov 2024

Viewed by 1692

Abstract

Mixed-species groups have been recorded in various primates, including tufted capuchin and squirrel monkeys. Measures of their ‘groupness’ are typically based on factors such as group stability, social interactions, proximity, or behavioural coordination. Social network analysis has become a useful tool for quantifying [...] Read more.

Mixed-species groups have been recorded in various primates, including tufted capuchin and squirrel monkeys. Measures of their ‘groupness’ are typically based on factors such as group stability, social interactions, proximity, or behavioural coordination. Social network analysis has become a useful tool for quantifying relationships among group-living individuals. Here, we apply social network analysis to two captive mixed-species groups of tufted capuchins and squirrel monkeys housed at the Living Links to Human Evolution Research Centre, Royal Zoological Society of Scotland, Edinburgh Zoo, UK. We conducted 183 h of focal observations (three hours per individual, excluding co-observations) and calculated association rates using a simple index ratio. Permutation t-tests were used to assess differences in the overall mixed-species network and network metrics according to species. While the two species exhibited some level of association, they formed separate clusters in the mixed-species networks; however, the East group had more balanced group sizes and showed some signs of closer inter-specific social ties compared to the West group. Our data indicate that, in captivity at least, while these groups co-exist in a small, shared space, they do not form cohesive mixed-species groups. We suggest caution in the assumption of mixed-species groups based on shared space only. Full article

(This article belongs to the Section Zoo Animals)

► Show Figures

Figure 1

22 pages, 6222 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Mitigating the Effects of Maternal Loss on Harbour Seal Pups in Captive Care

by Susan C. Wilson and Rhiannon Alger

Animals 2024, 14(22), 3264; https://doi.org/10.3390/ani14223264 - 13 Nov 2024

Viewed by 1149

Abstract

Stranded newborn “orphan” harbour seal pups entering captive care are often maintained for some weeks in isolation, mainly as a precaution against the spread of infection. However, this practice raises concerns for the welfare and normal socialisation of pups, who normally spend their [...] Read more.

Stranded newborn “orphan” harbour seal pups entering captive care are often maintained for some weeks in isolation, mainly as a precaution against the spread of infection. However, this practice raises concerns for the welfare and normal socialisation of pups, who normally spend their first post-natal weeks close to their mothers and other seals. The present study recorded and described the behaviour of six paired orphan pups in rehabilitation up to about five weeks of age, provided with free access to water and haul-out areas. The occurrences of resting, following, nosing and body contacts, and aquatic play were recorded and compared qualitatively and quantitatively with the same behaviours of free-living pups with their mothers. The pups entered the water every day, although more often from about 2.5 weeks of age. They displayed to each other the same behaviours that free-living pups display to their mothers, although they engaged in relatively more physical contact, body nosing, and aquatic play. The study has shown that orphan pups maintained in pairs with free water access can act reciprocally as mother substitutes, thereby promoting species-typical primary socialisation and welfare during their early days of captive care. Full article

(This article belongs to the Section Zoo Animals)

► Show Figures

Figure 1

22 pages, 7393 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Making Noah’s Ark Work for Fishing Cat Conservation: A Blueprint for Connecting Populations across an Interactive Wild Ex Situ Spectrum

by Tiasa Adhya, Simran Singh, Himaja Varma Gottumukkala, Aditya Banerjee, Ishita Chongder, Sulata Maity and P. Anuradha Reddy

Animals 2024, 14(19), 2770; https://doi.org/10.3390/ani14192770 - 25 Sep 2024

Viewed by 2904

Abstract

The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, [...] Read more.

The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, and emotional needs of captive individuals, thereby improving conservation outcomes. Here, we present an integrated framework for the conservation of a globally threatened wetland wild cat species, the fishing cat, by synthesizing optimal ex situ management practices and in situ conservation strategies. Further, we examined the genetic constitution of the founder population in a fishing cat captive breeding program that was recently initiated by the West Bengal Zoo Authority, India and conducted a population viability analysis to suggest how best to maintain the genetic diversity of the population. We found that the present genetic diversity of 56% and maximum carrying capacity of the captive population (30 individuals) can be maintained for more than 100 years with a combination of supplementation and harvesting. Keeping stochastic events in mind, the introduction of two adult males and females to the existing population each year will seamlessly allow the harvesting of two adult males and two adult females every alternate year to supplement wild populations. Further, we adopted the proposed integrated framework to delineate recommendations for the supplementation of wild populations in West Bengal. We used environmental criteria known to influence fishing cat occurrence to identify 21 potential reintroduction zones in the Sundarbans landscape and Terai region in northern West Bengal with habitable areas for the fishing cat that are larger than the maximum known species’ home range. Our study is timely and insightful because it provides a holistic blueprint for implementing the One Plan Approach in safeguarding a threatened species. Full article

(This article belongs to the Special Issue Zoo Animals: How Actual Zoological Institutions Assess, Ensure, and Promote Their Animals’ Welfare?)

► Show Figures

Figure 1

9 pages, 1715 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

The Determination of the Prohibited Herbicide 4,6-Dinitro-Ortho-Cresol (DNOC) in Poisoned Domestic and Wild Animals in Italy

by Rachele Rocchi, Antonella Tinelli, Giuseppe Gatti, Pietro Badagliacca, Antonio Cocco, Maria Chiara Cantelmi, Antonella Damiano, Giampiero Scortichini, Carmine Merola and Antonio Petrini

Animals 2024, 14(17), 2483; https://doi.org/10.3390/ani14172483 - 26 Aug 2024

Viewed by 1705

Abstract

This study reports the determination of DNOC in the baits and gastric contents of poisoned dogs and wild canids collected in the Abruzzo region between 2014 and 2022. A total of 663 samples of the baits and carcasses of domestic and wild animals [...] Read more.

This study reports the determination of DNOC in the baits and gastric contents of poisoned dogs and wild canids collected in the Abruzzo region between 2014 and 2022. A total of 663 samples of the baits and carcasses of domestic and wild animals were analyzed for the determination of DNOC through GC-MS. DNOC was found to be present in 58 cases from 663 analyzed samples, with 49 poison baits and nine gastric content samples from dogs and red foxes. This study confirms for the first time that the banned pesticide DNOC still poisons both domestic and wild animals in the Abruzzo region. It should be considered that this study was carried out on a population of animals from a regional geographical area, and more detailed country-wide studies need to be carried out to obtain the incidence of poisoning from this herbicide in Italy. Furthermore, these findings emphasize the importance of considering this chemical in differential diagnosis during toxicological investigations of animal poisoning. Full article

(This article belongs to the Special Issue Environmental Toxicology: The Effects of Chemical Exposure on Animal Physiological and Biochemical Indicators)

► Show Figures

Figure 1

14 pages, 11156 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Chromium Affects Mitochondrial Function, Leading to Apoptosis and Autophagy in Turtle Primary Hepatocytes

by Shuqin Lin, Yunjuan Xiao, Jing Lin, Yue Yuan, Haitao Shi, Meiling Hong and Li Ding

Animals 2024, 14(16), 2403; https://doi.org/10.3390/ani14162403 - 19 Aug 2024

Cited by 1 | Viewed by 1911

Abstract

Hexavalent chromium (Cr(VI)), a pervasive industrial contaminant, is highly toxic to both humans and animals. However, its effects on turtles are largely unexplored. Our study aimed to investigate the toxic effects of Cr(VI) on the Reeves’ turtles (Mauremys reevesii) primary hepatocytes. [...] Read more.

Hexavalent chromium (Cr(VI)), a pervasive industrial contaminant, is highly toxic to both humans and animals. However, its effects on turtles are largely unexplored. Our study aimed to investigate the toxic effects of Cr(VI) on the Reeves’ turtles (Mauremys reevesii) primary hepatocytes. We exposed hepatocytes to two concentrations (25 μM and 50 μM) of Cr(VI) for 24 h. The results showed that compared to controls, Cr(VI)-treated cells showed elevated antioxidant enzyme activity (catalase (CAT) and superoxide dismutase (SOD)) and increased reactive oxygen species (ROS) levels. Adenosine triphosphatae (ATP) levels decreased, indicating mitochondrial dysfunction. Additionally, we found significant changes in mitochondrial dynamics related genes, with downregulation of mitofusin 2 (Mfn2) and silent information regulator 1 (SIRT1) and a decrease in sirtuin 3 (SIRT3) and tumor protein 53 (p53) mRNA levels. Annexin V-FITC fluorescence staining-positive cells increased with higher Cr(VI) concentrations, marked by elevated bcl-2-associated X protein (Bax) and cysteinyl aspartate specific proteinase (Caspase3) mRNA levels and reduced B-cell lymphoma-2 (Bcl2) expression. Autophagy-related genes were also affected, with increased microtubule-associated protein 1 light chain 3 (LC3-I), microtubule-associated protein light chain 3II (LC3-II), unc-51-like autophagy-activating kinase 1 (ULK1), and sequestosome 1 (p62/SQSTM1) mRNA levels and decreased mammalian target of rapamycin (mTOR) and Beclin1 expression. Taken together, Cr(VI) promotes cell apoptosis and autophagy in turtle hepatocytes by inducing oxidative stress and disrupting mitochondrial function. These findings highlight the serious health risks posed by Cr(VI) pollution and emphasize the need for protecting wild turtle populations. Full article

(This article belongs to the Special Issue Aquatic Animal Medicine and Pathology)

► Show Figures

Figure 1

20 pages, 18545 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Stress Responses in Horses Housed in Different Stable Designs during Summer in a Tropical Savanna Climate

by Chanoknun Poochipakorn, Thita Wonghanchao, Kanokpan Sanigavatee and Metha Chanda

Animals 2024, 14(15), 2263; https://doi.org/10.3390/ani14152263 - 4 Aug 2024

Cited by 1 | Viewed by 1912

Abstract

Single-confinement housing can pose welfare risks to domestic horses. This study investigated horses’ stress responses when confined to single stalls in different stable designs in a tropical savanna region to address a gap in the literature. In total, 23 horses were assigned to [...] Read more.

Single-confinement housing can pose welfare risks to domestic horses. This study investigated horses’ stress responses when confined to single stalls in different stable designs in a tropical savanna region to address a gap in the literature. In total, 23 horses were assigned to a stable with a central corridor and solid external walls (A) (N = 8), a stable with one side corridor and solid external walls (B) (N = 6), or a stable with a central corridor and no solid external walls (C) (N = 9). Air velocity, relative humidity, air temperature, and noxious gases were measured inside the stables, and the heart rate and HRV of the horses were also determined. The relative humidity was lower in stable C than in stable A (p < 0.05), while the air temperature was higher in stable C than in stable B (p < 0.05) during the day. The airflow and ammonia levels were higher in stable C than in stables B and A (p < 0.01–0.0001). Overall, horses’ HRV in stable A was lower than in those in stables B and C (p < 0.05–0.01). Horses in stable A tended to experience more stress than those in other stables. Full article

(This article belongs to the Special Issue Exploring Stress Responses in Farm Animals: A Focus on Physiological and Behavioral Aspects)

► Show Figures

Figure 1

11 pages, 1990 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

The Interplay of TLR-NFκB Signalling Pathway and Functional Immune-Related Enzymes in the Inflammatory Response of Ciona robusta

by Luca Bisanti, Claudia La Corte, Mariano Dara, Federica Bertini, Jacopo Vizioli, Maria Giovanna Parisi, Matteo Cammarata and Daniela Parrinello

Animals 2024, 14(15), 2169; https://doi.org/10.3390/ani14152169 - 25 Jul 2024

Cited by 2 | Viewed by 1348

Abstract

The close phylogenetic relationship between ascidians (Tunicata) and vertebrates makes them a powerful model for studying the innate immune system. To better understand the nature and dynamics of immune responses and the mechanisms through which bacterial infections are detected and translated into inflammation [...] Read more.

The close phylogenetic relationship between ascidians (Tunicata) and vertebrates makes them a powerful model for studying the innate immune system. To better understand the nature and dynamics of immune responses and the mechanisms through which bacterial infections are detected and translated into inflammation in Ciona robusta, we applied an approach combining in vivo lipopolysaccharide (LPS) stimulation, immune-labelling techniques and functional enzymatic analyses. The immunohistochemistry showed that Toll-like receptor 4 (TLR4) and nuclear factor kappa B (NFκB) were expressed during the inflammatory pharynx response 4 h post-LPS, with the formation of nodules in pharynx vessel lumen. Also, the endothelium vessels were involved in the inflammatory response. Observations of histological sections from naive and buffer-inoculated ascidians confirmed an immuno-positive response. Enzyme immune parameters—which included the activity of phenoloxidase, glutathione peroxidase, lysozyme, alkaline phosphatase and esterase—showed up-modulation 4 h after LPS injection, confirming their participation during ascidian inflammatory response. These findings provide new insights into the mechanisms underlying the LPS-induced C. robusta response and suggest that a broad innate immune mechanism, as in vertebrates, is involved in the regulation of inflammatory responses. Further findings in this direction are needed to cover knowledge gaps regarding the organized set of molecular and cellular networks involved in universal immune interactions with pathogens. Full article

(This article belongs to the Section Aquatic Animals)

► Show Figures

Figure 1

17 pages, 1645 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Different Founding Effects Underlie Dominant Blue Eyes (DBE) in the Domestic Cat

by Marie Abitbol, Caroline Dufaure de Citres, Gabriela Rudd Garces, Gesine Lühken, Leslie A. Lyons and Vincent Gache

Animals 2024, 14(13), 1845; https://doi.org/10.3390/ani14131845 - 21 Jun 2024

Cited by 1 | Viewed by 6251

Abstract

During the last twenty years, minimal white spotting associated with blue eyes was selected by feline breeders to create the Altai, Topaz, and Celestial breeds. Additionally, certain breeders introduced this trait in their lineages of purebred cats. The trait has been called “dominant [...] Read more.

During the last twenty years, minimal white spotting associated with blue eyes was selected by feline breeders to create the Altai, Topaz, and Celestial breeds. Additionally, certain breeders introduced this trait in their lineages of purebred cats. The trait has been called “dominant blue eyes (DBE)” and was confirmed to be autosomal dominant in all lineages. DBE was initially described in outbred cats from Kazakhstan and Russia and in two purebred lineages of British cats from Russia, as well as in Dutch Maine Coon cats, suggesting different founding effects. We have previously identified two variants in the Paired Box 3 (PAX3) gene associated with DBE in Maine Coon and Celestial cats; however, the presence of an underlying variant remains undetermined in other DBE breeding lines. Using a genome-wide association study, we identified a single region on chromosome C1 that was associated with DBE in British cats. Within that region, we identified PAX3 as the strongest candidate gene. Whole-genome sequencing of a DBE cat revealed an RD-114 retrovirus LTR (long terminal repeat) insertion within PAX3 intron 4 (namely NC_018730.3:g.206975776_206975777insN[433]) known to contain regulatory sequences. Using a panel of 117 DBE cats, we showed that this variant was fully associated with DBE in two British lineages, in Altai cats, and in some other DBE lineages. We propose that this NC_018730.3:g.206975776_206975777insN[433] variant represents the DBE^ALT (Altai Dominant Blue Eye) allele in the domestic cat. Finally, we genotyped DBE cats from 14 lineages for the three PAX3 variants and showed that they were not present in four lineages, confirming genetic heterogeneity of the DBE trait in the domestic cat. Full article

(This article belongs to the Section Animal Genetics and Genomics)

► Show Figures

Figure 1

11 pages, 2290 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Comparative Cytogenetics of the Malagasy Ground Geckos of the Paroedura bastardi and Paroedura picta Species Groups

by Marcello Mezzasalma, Gaetano Odierna, Rachele Macirella and Elvira Brunelli

Animals 2024, 14(11), 1708; https://doi.org/10.3390/ani14111708 - 6 Jun 2024

Viewed by 1212

Abstract

We present a comparative chromosome study of several taxa of the Malagasy ground geckos of the Paroedura bastardi and P. picta species groups. We employed a preliminary molecular analysis using a trait of the mitochondrial 16S rRNA gene (of about 570 bp) to [...] Read more.

We present a comparative chromosome study of several taxa of the Malagasy ground geckos of the Paroedura bastardi and P. picta species groups. We employed a preliminary molecular analysis using a trait of the mitochondrial 16S rRNA gene (of about 570 bp) to assess the taxonomic status of the samples studied and a cytogenetic analysis with standard karyotyping (5% Giemsa solution), silver staining (Ag–NOR staining) and sequential C-banding (C-banding + Giemsa and + fluorochromes). Our results show that all the taxa studied of the P. bastardi group (P. ibityensis, P. rennerae and P. cf. guibeae) have a similar karyotype composed of 2n = 34 chromosomes, with two metacentric pairs (1 and 3) and all other pairs being acrocentric. Chromosome diversification in the P. bastardi group was mainly linked to the diversification of heteromorphic sex chromosome systems (ZZ/ZW) in P. ibityensis and P. rennerae, while no heteromorphic sex chromosome pair was found in P. cf. guibeae. The two taxa investigated of the P. picta species group (here named P. picta and P. cf. picta based on molecular data) showed the same chromosome number of 2n = 36, mostly acrocentric elements, but differed in the number of metacentric elements, probably as a result of an inversion at chromosome pair 2. We highlight that the genus Paroedura is characterized by the independent diversification of heterogametic sex chromosomes in different evolutionary lineages and, similarly to other phylogenetically related gecko genera, by a progressive formation of a biarmed element by means of tandem fusions and inversions of distinct pairs. Full article

(This article belongs to the Section Animal Genetics and Genomics)

► Show Figures

Figure 1

16 pages, 1004 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

A Study on Mineral Oil Hydrocarbons (MOH) Contamination in Pig Diets and Its Transfer to Back Fat and Loin Tissues

by Paula Albendea, Chiara Conchione, Luca Menegoz Ursol and Sabrina Moret

Animals 2024, 14(10), 1450; https://doi.org/10.3390/ani14101450 - 13 May 2024

Cited by 2 | Viewed by 1876

Abstract

This study assessed saturated mineral oil hydrocarbons (MOSH) and aromatic mineral oil hydrocarbons (MOAH) levels in grower–finisher feeds for pigs supplemented with 5% crude palm oil (CP), crude olive pomace oil (COP), olive pomace acid oil (OPA), or a blend of CP and [...] Read more.

This study assessed saturated mineral oil hydrocarbons (MOSH) and aromatic mineral oil hydrocarbons (MOAH) levels in grower–finisher feeds for pigs supplemented with 5% crude palm oil (CP), crude olive pomace oil (COP), olive pomace acid oil (OPA), or a blend of CP and OPA (50:50, w/w); the contribution of the lipid source to that contamination; and the ability of pigs to accumulate MOH in back fat and loin tissues after 60 days of trial. MOSH and MOAH were analyzed with liquid chromatography (LC)–gas chromatography (GC)–flame ionization detection (FID) after sample preparation. Among the lipid sources, CP had the lowest MOH levels, but CP feeds showed the highest contamination. This, along with the different MOSH profiles, indicated the presence of more significant contamination sources in the feeds than the lipid source. The higher MOH contamination in CP feeds was reflected in the highest MOSH levels in pig back fat, whereas MOAH were not detected in animal tissues. Also, MOSH bioaccumulation in pig tissues was influenced by the carbon chain length. In conclusion, feed manufacturing processes can determine the MOSH contamination present in animal adipose tissues that can be included in human diets. Full article

(This article belongs to the Special Issue The Influence of Environment and Management on the Animal-Origin Products)

► Show Figures

Figure 1

23 pages, 6104 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Maternal Effects and Trophodynamics Drive Interannual Larval Growth Variability of Atlantic Bluefin Tuna (Thunnus thynnus) from the Gulf of Mexico

by José M. Quintanilla, Ricardo Borrego-Santos, Estrella Malca, Rasmus Swalethorp, Michael R. Landry, Trika Gerard, John Lamkin, Alberto García and Raúl Laiz-Carrión

Animals 2024, 14(9), 1319; https://doi.org/10.3390/ani14091319 - 28 Apr 2024

Viewed by 1535

Abstract

Two cohorts of Atlantic bluefin tuna (Thunnus thynnus) larvae were sampled in 2017 and 2018 during the peak of spawning in the Gulf of Mexico (GOM). We examined environmental variables, daily growth, otolith biometry and stable isotopes and found that the [...] Read more.

Two cohorts of Atlantic bluefin tuna (Thunnus thynnus) larvae were sampled in 2017 and 2018 during the peak of spawning in the Gulf of Mexico (GOM). We examined environmental variables, daily growth, otolith biometry and stable isotopes and found that the GOM18 cohort grew at faster rates, with larger and wider otoliths. Inter and intra-population analyses (deficient vs. optimal growth groups) were carried out for pre- and post-flexion developmental stages to determine maternal and trophodynamic influences on larval growth variability based on larval isotopic signatures, trophic niche sizes and their overlaps. For the pre-flexion stages in both years, the optimal growth groups had significantly lower δ¹⁵N, implying a direct relationship between growth potential and maternal inheritance. Optimal growth groups and stages for both years showed lower C:N ratios, reflecting a greater energy investment in growth. The results of this study illustrate the interannual transgenerational trophic plasticity of a spawning stock and its linkages to growth potential of their offsprings in the GOM. Full article

(This article belongs to the Special Issue Fisheries Larval Ecology and Oceanography)

► Show Figures

Figure 1

16 pages, 866 KiB

Open AccessEditor’s ChoiceArticle

Effects of Garlic Oil and Cinnamaldehyde on Sheep Rumen Fermentation and Microbial Populations in Rusitec Fermenters in Two Different Sampling Periods

by Jairo García-Rodríguez, Cristina Saro, Iván Mateos, María Dolores Carro and María José Ranilla

Animals 2024, 14(7), 1067; https://doi.org/10.3390/ani14071067 - 30 Mar 2024

Viewed by 2060

Abstract

Garlic oil (GO) and cinnamaldehyde (CIN) have shown potential to modify rumen fermentation. The aim of this study was to assess the effects of GO and CIN on rumen fermentation, microbial protein synthesis (MPS), and microbial populations in Rusitec fermenters fed a mixed [...] Read more.

Garlic oil (GO) and cinnamaldehyde (CIN) have shown potential to modify rumen fermentation. The aim of this study was to assess the effects of GO and CIN on rumen fermentation, microbial protein synthesis (MPS), and microbial populations in Rusitec fermenters fed a mixed diet (50:50 forage/concentrate), as well as whether these effects were maintained over time. Six fermenters were used in two 15-day incubation runs. Within each run, two fermenters received no additive, 180 mg/L of GO, or 180 mg/L of CIN. Rumen fermentation parameters were assessed in two periods (P1 and P2), and microbial populations were studied after each of these periods. Garlic oil reduced the acetate/propionate ratio and methane production (p < 0.001) in P1 and P2 and decreased protozoal DNA concentration and the relative abundance of fungi and archaea after P1 (p < 0.05). Cinnamaldehyde increased bacterial diversity (p < 0.01) and modified the structure of bacterial communities after P1, decreased bacterial DNA concentration after P2 (p < 0.05), and increased MPS (p < 0.001). The results of this study indicate that 180 mg/L of GO and CIN promoted a more efficient rumen fermentation and increased the protein supply to the animal, respectively, although an apparent adaptive response of microbial populations to GO was observed. Full article

(This article belongs to the Section Animal Nutrition)

► Show Figures

Figure 1

10 pages, 289 KiB

Open AccessEditor’s ChoiceArticle

Evaluation of Two Species of Macroalgae from Azores Sea as Potential Reducers of Ruminal Methane Production: In Vitro Ruminal Assay

by Helder P. B. Nunes, Cristiana S. A. M. Maduro Dias, Nuno V. Álvaro and Alfredo E. S. Borba

Animals 2024, 14(6), 967; https://doi.org/10.3390/ani14060967 - 20 Mar 2024

Cited by 7 | Viewed by 2361

Abstract

The utilisation of seaweeds as feed supplements has been investigated for their potential to mitigate enteric methane emissions from ruminants. Enteric methane emissions are the primary source of direct greenhouse gas emissions in livestock and significantly contribute to anthropogenic methane emissions worldwide. The [...] Read more.

The utilisation of seaweeds as feed supplements has been investigated for their potential to mitigate enteric methane emissions from ruminants. Enteric methane emissions are the primary source of direct greenhouse gas emissions in livestock and significantly contribute to anthropogenic methane emissions worldwide. The aim of the present study is to evaluate the nutritional role and the in vitro effect on cumulative gas and methane production of Asparagopsis taxiformis (native species) and Asparagopsis armata (invasive species), two species of red algae from the Azorean Sea, as well as the ability to reduce biogas production when incubated with single pasture (Lolium perenne and Trifollium repens) as substrate. Four levels of concentrations marine algae were used (1.25%, 2.25%, 5%, and 10% DM) and added to the substrate to evaluate ruminal fermentation using the in vitro gas production technique. The total amount of gas and methane produced by the treatment incubation was recorded during 72 h of incubation. The results indicate that both algae species under investigation contain relatively high levels of protein (22.69% and 24.23%, respectively, for Asparagopsis taxiformis and Asparagopsis armata) and significant amounts of minerals, namely magnesium (1.15% DM), sodium (8.6% DM), and iron (2851 ppm). Concerning in vitro ruminal fermentation, it was observed that A. taxiformis can reduce enteric methane production by approximately 86%, during the first 24 h when 5% is added. In the same period and at the same concentration, A. armata reduced methane production by 34%. Thus, it can be concluded that Asparagopsis species from the Azorean Sea have high potential as a protein and mineral supplement, in addition to enabling a reduction in methane production from rumen fermentation. Full article

(This article belongs to the Special Issue Algae in Animal Nutrition)

► Show Figures

Figure 1

10 pages, 947 KiB

Open AccessEditor’s ChoiceArticle

Effects of Sugarcane-Derived Polyphenol Supplementation on Methane Production and Rumen Microbial Diversity of Second-Cross Lambs

by Pragna Prathap, Surinder S. Chauhan, Matthew Flavel, Shane Mitchell, Jeremy J. Cottrell, Brian J. Leury and Frank R. Dunshea

Animals 2024, 14(6), 905; https://doi.org/10.3390/ani14060905 - 14 Mar 2024

Cited by 7 | Viewed by 2548

Abstract

The objective of this study was to evaluate the effects of feeding sugarcane-derived polyphenolic supplement (Polygain, The Product Makers Australia, Keysborough, VIC, Australia) on enteric methane (CH₄) emission, rumen microbiota, and performance of second-cross lambs. For this purpose, 24 Poll Dorset [...] Read more.

The objective of this study was to evaluate the effects of feeding sugarcane-derived polyphenolic supplement (Polygain, The Product Makers Australia, Keysborough, VIC, Australia) on enteric methane (CH₄) emission, rumen microbiota, and performance of second-cross lambs. For this purpose, 24 Poll Dorset × (Border Leicester × Merino) lambs were allocated to 3 different treatments: Control (C), 0.25% Polygain (0.25 PG), and 1% Polygain (1 PG) diets with a uniform basal feed (25% cracked wheat grain, 25% cracked barley grain, 25% oaten chaff, 25% lucerne chaff). Both doses of Polygain reduced the total CH₄ production (g/day; p = 0.006), CH₄ yield (CH₄, g/kg of dry matter intake; p = 0.003) and CH₄ intensity (CH₄, g/kg of BW; p = 0.003). Dry matter intake tended to be greater (p = 0.08) in sheep fed 1 PG compared to the C group, with the 0.25 PG group being intermediate. The average daily gain of the lambs was improved (p = 0.03) with 1% Polygain supplementation. The relative abundance of genera Methanobrevibacter_unidentified, Methanomethylophilaceae_uncultured, Methanogenic archaeon mixed culture ISO4-G1, Methanosphaera uncultured rumen methanogen, Methanogenic archaeon ISO4-H5, and Methanobrevibacter boviskoreani JH1 were reduced with Polygain supplementation. In conclusion, feeding Polygain reduced lambs’ enteric CH₄ emissions, altered the rumen microbiome, and improved the growth performance of lambs. Full article

(This article belongs to the Section Animal Nutrition)

► Show Figures

Figure 1

14 pages, 2035 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Influence of Different Plant Extracts on CYP-Mediated Skatole and Indole Degradation in Pigs

by Philipp Marro, Raffael Wesoly and Volker Stefanski

Animals 2024, 14(6), 888; https://doi.org/10.3390/ani14060888 - 13 Mar 2024

Cited by 3 | Viewed by 1819

Abstract

One of the primary substances responsible for the unpleasant odor in boar meat is skatole. Enzymes belonging to the cytochrome P450 (CYP) family play a pivotal role in the hepatic clearance of skatole. This study aimed to investigate the impact of oregano essential [...] Read more.

One of the primary substances responsible for the unpleasant odor in boar meat is skatole. Enzymes belonging to the cytochrome P450 (CYP) family play a pivotal role in the hepatic clearance of skatole. This study aimed to investigate the impact of oregano essential oil (OEO), Schisandra chinensis extract (SC), and garlic essential oil (GEO) on hepatic CYP2E1 and CYP2A activity in pigs. In three consecutive trials, cannulated castrated male pigs were provided with a diet containing 0.2–0.3% of one of these plant extracts. Following a 14-day feeding period, the animals were slaughtered, and liver and fat samples were collected. The findings indicate that the activities of CYP2E1 were unaffected by any treatment. However, GEO treatment demonstrated a significant reduction in CYP2A activity (p < 0.05). Pigs treated with GEO also exhibited a notable increase in skatole concentrations in both plasma and adipose tissue. In contrast, animals fed SC displayed elevated skatole concentrations in plasma but not in fat tissue. OEO did not influence skatole concentrations in either blood or fat. Furthermore, the study revealed that a supplementation of 6 g GEO per animal per day induced a significant increase in skatole concentrations in blood plasma within 24 h. Full article

(This article belongs to the Special Issue Plant Extracts as Feed Additives in Animal Nutrition and Health)

► Show Figures

Figure 1

16 pages, 3470 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis

by Rafael González-Cano, Ana González-Martínez, Manuel Ramón, Miriam González Serrano, Miguel Moreno Millán, Alejandro Rubio de Juan and Evangelina Rodero Serrano

Animals 2024, 14(5), 793; https://doi.org/10.3390/ani14050793 - 4 Mar 2024

Viewed by 1897

Abstract

Most of the previous studies on the genetic variability in Spanish “Berrenda” breeds have been carried out using DNA microsatellites. The present work aimed to estimate the genetic diversity, population structure, and potential genetic differences among individuals of both Berrenda breeds and groups [...] Read more.

Most of the previous studies on the genetic variability in Spanish “Berrenda” breeds have been carried out using DNA microsatellites. The present work aimed to estimate the genetic diversity, population structure, and potential genetic differences among individuals of both Berrenda breeds and groups based on the presence of the Robertsonian chromosomal translocation, rob (1;29). A total of 373 samples from animals belonging to the two breeds, including 169 cases diagnosed as rob (1;29)-positive, were genotyped using an SNP50K chip. The genetic diversity at the breed level did not show significant differences, but it was significantly lower in those subpopulations containing the rob (1;29). Runs of homozygosity identified a region of homozygosity on chromosome 6, where the KIT (KIT proto-oncogene, receptor tyrosine kinase) gene, which determines the typical spotted coat pattern in both breeds, is located. The four subpopulations considered showed minor genetic differences. The regions of the genome that most determined the differences between the breeds were observed on chromosomes 4, 6, 18, and 22. The presence of this Robertsonian translocation did not result in sub-structuring within each of the breeds considered. To improve the reproductive performance of Berrenda breeds, it would be necessary to implement strategies considering the involvement of potential breeding stock carrying rob (1;29). Full article

(This article belongs to the Section Animal Genetics and Genomics)

► Show Figures

Figure 1

13 pages, 233 KiB

Open AccessEditor’s ChoiceArticle

Estimation of Protein and Amino Acid Requirements in Layer Chicks Depending on Dynamic Model

by Miao Liu, Zhi-Yuan Xia, Hong-Lin Li, Yu-Xuan Huang, Alainaa Refaie, Zhang-Chao Deng and Lv-Hui Sun

Animals 2024, 14(5), 764; https://doi.org/10.3390/ani14050764 - 29 Feb 2024

Cited by 5 | Viewed by 2610

Abstract

Four trials were conducted to establish a protein and amino acid requirement model for layer chicks over 0–6 weeks by using the analytical factorization method. In trial 1, a total of 90 one-day-old Jing Tint 6 chicks with similar body weight were selected [...] Read more.

Four trials were conducted to establish a protein and amino acid requirement model for layer chicks over 0–6 weeks by using the analytical factorization method. In trial 1, a total of 90 one-day-old Jing Tint 6 chicks with similar body weight were selected to determine the growth curve, carcass and feather protein deposition, and amino acid patterns of carcass and feather proteins. In trials 2 and 3, 24 seven-day-old and 24 thirty-five-day-old Jing Tint 6 chicks were selected to determine the protein maintenance requirements, amino acid pattern, and net protein utilization rate. In trial 4, 24 ten-day-old and 24 thirty-eight-day-old Jing Tint 6 chicks were selected to determine the standard terminal ileal digestibility of amino acids. The chicks were fed either a corn–soybean basal diet, a low nitrogen diet, or a nitrogen-free diet throughout the different trials. The Gompertz equation showed that there is a functional relationship between body weight and age, described as BWt(g) = 2669.317 × exp(−4.337 × exp(−0.019t)). Integration of the test results gave a comprehensive dynamic model equation that could accurately calculate the weekly protein and amino acid requirements of the layer chicks. By applying the model, it was found that the protein requirements for Jing Tint 6 chicks during the 6-week period were 21.15, 20.54, 18.26, 18.77, 17.79, and 16.51, respectively. The model-predicted amino acid requirements for Jing Tint 6 chicks during the 6-week period were as follows: Aspartic acid (0.992–1.284), Threonine (0.601–0.750), Serine (0.984–1.542), Glutamic acid (1.661–1.925), Glycine (0.992–1.227), Alanine (0.909–0.961), Valine (0.773–1.121), Cystine (0.843–1.347), Methionine (0.210–0.267), Isoleucine (0.590–0.715), Leucine (0.977–1.208), Tyrosine (0.362–0.504), Phenylalanine (0.584–0.786), Histidine (0.169–0.250), Lysine (0.3999–0.500), Arginine (0.824–1.147), Proline (1.114–1.684), and Tryptophan (0.063–0.098). In conclusion, this study constructed a dynamic model for the protein and amino acid requirements of Jing Tint 6 chicks during the brooding period, providing an important insight to improve precise feeding for layer chicks through this dynamic model calculation. Full article

(This article belongs to the Special Issue Amino Acid Nutrition in Poultry)

24 pages, 319 KiB

Open AccessEditor’s ChoiceArticle

A Reexamination of the Relationship between Training Practices and Welfare in the Management of Ambassador Animals

by Steve Martin, Grey Stafford and David S. Miller

Animals 2024, 14(5), 736; https://doi.org/10.3390/ani14050736 - 27 Feb 2024

Cited by 2 | Viewed by 5560

Abstract

There is an ethical need to document and develop best practices for meeting ambassador animals’ welfare needs within the context of meeting zoo and aquarium program objectives. This is because ambassador animals experience direct and frequent contact with humans. This paper rigorously synthesizes [...] Read more.

There is an ethical need to document and develop best practices for meeting ambassador animals’ welfare needs within the context of meeting zoo and aquarium program objectives. This is because ambassador animals experience direct and frequent contact with humans. This paper rigorously synthesizes behavioral research and theory, contemporary practices, and personal experiences to offer key concepts that can be applied to meet ambassador animal welfare needs. These key concepts include addressing an animal’s recognition of choice and control, the use of the most positive and least intrusive effective interventions when training animals to participate in programming, and an overall reduction in aversive strategy use. Our model for increasing ambassador animal welfare focuses on seven main areas of concern, including the following: choosing the most suitable animal for the program; choosing the human with the right skills and knowledge for the program; using the most positive, least intrusive, effective training methods; developing a strong trusting relationship between trainer and animal; developing a comprehensive enrichment program; the need for institutional support; and creating opportunities for animals to practice species-appropriate behaviors. Our model will provide guidelines for improved ambassador animal welfare that can be refined with future research. Full article

(This article belongs to the Special Issue Zoo and Aquarium Welfare, Ethics, Behavior)

23 pages, 3187 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Dietary Protein Quality Affects the Interplay between Gut Microbiota and Host Performance in Nile Tilapia

by Gabriella do Vale Pereira, Carla Teixeira, José Couto, Jorge Dias, Paulo Rema and Ana Teresa Gonçalves

Animals 2024, 14(5), 714; https://doi.org/10.3390/ani14050714 - 24 Feb 2024

Cited by 6 | Viewed by 3210

Abstract

Dietary protein quality plays a key role in maintaining intestinal mucosal integrity, but also modulates the growth of luminal microorganisms. This work assessed the effect of dietary protein sources on the performance, gut morphology, and microbiome in Nile tilapia. Four isonitrogenous and isolipidic [...] Read more.

Dietary protein quality plays a key role in maintaining intestinal mucosal integrity, but also modulates the growth of luminal microorganisms. This work assessed the effect of dietary protein sources on the performance, gut morphology, and microbiome in Nile tilapia. Four isonitrogenous and isolipidic diets comprising equivalent amounts of the protein supply derived from either PLANT, ANIMAL, INSECT, or BACTERIAL (bacterial biomass) sources were fed to triplicate groups of fish (IBW: 12 g) for 46 days. Fish fed the ANIMAL and BACTERIAL diets showed significantly higher weight gains than those fed the PLANT and INSECT diets (p < 0.05). Relative abundance at the phylum level showed that Bacteroidetes, Fusobacteria, and Proteobacteria were the more abundant phyla in tilapia’s intestine, while Cetobacterium was the most representative genus in all treatments. Interesting patterns were observed in the correlation between amino acid intake and genus and species abundance. Metabolism prediction analysis showed that BACTERIAL amine and polyamine degradation pathways are modulated depending on diets. In conclusion, different protein sources modulate the relationship between bacteria functional pathways and amino acid intake. Full article

(This article belongs to the Special Issue Feed Ingredients and Fish Mucosal Health)

► Show Figures

Figure 1

32 pages, 1417 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Dolphin-WET—Development of a Welfare Evaluation Tool for Bottlenose Dolphins (Tursiops truncatus) under Human Care

by Katrin Baumgartner, Tim Hüttner, Isabella L. K. Clegg, Manuel Garcia Hartmann, Daniel Garcia-Párraga, Xavier Manteca, Birgitta Mercera, Tania Monreal-Pawlowsky, Cristina Pilenga, Kerstin Ternes, Oriol Tallo-Parra, Ruta Vaicekauskaite, Lorenzo von Fersen, Lisa Yon and Fabienne Delfour

Animals 2024, 14(5), 701; https://doi.org/10.3390/ani14050701 - 23 Feb 2024

Cited by 3 | Viewed by 5606

Abstract

Ensuring high standards of animal welfare is not only an ethical duty for zoos and aquariums, but it is also essential to achieve their conservation, education, and research goals. While for some species, animal welfare assessment frameworks are already in place, little has [...] Read more.

Ensuring high standards of animal welfare is not only an ethical duty for zoos and aquariums, but it is also essential to achieve their conservation, education, and research goals. While for some species, animal welfare assessment frameworks are already in place, little has been done for marine animals under human care. Responding to this demand, the welfare committee of the European Association for Aquatic Mammals (EAAM) set up a group of experts on welfare science, cetacean biology, and zoo animal medicine across Europe. Their objective was to develop a comprehensive tool to evaluate the welfare of bottlenose dolphins (Tursiops truncatus), named Dolphin-WET. The tool encompasses 49 indicators that were either validated through peer review or management-based expertise. The first of its kind, the Dolphin-WET is a species-specific welfare assessment tool that provides a holistic approach to evaluating dolphin welfare. Inspired by Mellor’s Five Domains Model and the Welfare Quality^®, its hierarchical structure allows for detailed assessments from overall welfare down to specific indicators. Through combining 37 animal-based and 12 resource-based indicators that are evaluated based on a two- or three-level scoring, the protocol offers a detailed evaluation of individual dolphins. This approach allows for regular internal monitoring and targeted welfare management, enabling caretakers to address specific welfare concerns effectively. Full article

(This article belongs to the Special Issue Advances in Marine Mammal Cognition and Cognitive Welfare)

► Show Figures

Figure 1

31 pages, 13259 KiB

Open AccessEditor’s ChoiceArticle

Impact of Climate Change on the Distribution of Three Rare Salamanders (Liua shihi, Pseudohynobius jinfo, and Tylototriton wenxianensis) in Chongqing, China, and Their Conservation Implications

by Qi Ma, Lipeng Wan, Shengchao Shi and Zhijian Wang

Animals 2024, 14(5), 672; https://doi.org/10.3390/ani14050672 - 21 Feb 2024

Cited by 9 | Viewed by 2408

Abstract

The Wushan Salamander (Liua shihi), Jinfo Salamander (Pseudohynobius jinfo), and Wenxian Knobby Salamander (Tylototriton wenxianensis) are rare national Class II protected wild animals in China. We performed MaxEnt modeling to predict and analyze the potential distribution and [...] Read more.

The Wushan Salamander (Liua shihi), Jinfo Salamander (Pseudohynobius jinfo), and Wenxian Knobby Salamander (Tylototriton wenxianensis) are rare national Class II protected wild animals in China. We performed MaxEnt modeling to predict and analyze the potential distribution and trends of these species in Chongqing under current and future climate conditions. Species distribution data were primarily obtained from field surveys, supplemented by museum collections and the existing literature. These efforts yielded 636 records, including 43 for P. jinfo, 23 for T. wenxianensis, and 570 for L. shihi. Duplicate records within the same 100 m × 100 m grid cell were removed using ENMTools, resulting in 10, 12, and 58 valid distribution points for P. jinfo, T. wenxianensis, and L. shihi, respectively. The optimization of feature class parameters (FC) and the regularization multiplier (RM) were applied using R package “ENMeval 2.0” to establish the optimal model with MaxEnt. The refined models were applied to simulate the suitable distribution areas for the three species. The results indicate that the current suitable habitat area for L. shihi accounted for 9.72% of the whole region of the Chongqing municipality. It is projected that, by 2050, the proportion of suitable habitat will increase to 12.54% but will decrease to 11.98% by 2070 and further decline to 8.80% by 2090. The current suitable habitat area for P. jinfo accounted for 1.08% of the whole region of the Chongqing municipality, which is expected to decrease to 0.31%% by 2050, 0.20% by 2070, and 0.07% by 2090. The current suitable habitat area for T. wenxianensis accounted for 0.81% of the whole region of the Chongqing municipality, which is anticipated to decrease to 0.37% by 2050, 0.21% by 2070, and 0.06% by 2090. Human disturbance, climate variables, and habitat characteristics are the primary factors influencing the distribution of three salamander species in Chongqing. The proximity to roads significantly impacts L. shihi, while climate conditions mainly affect P. jinfo, and the distance to water sources is crucial for T. wenxianensis. The following suggestions were made based on key variables identified for each species: (1) For L. shihi, it is imperative to minimize human disturbances and preserve areas without roads and the existing vegetation within nature reserves to ensure their continued existence. (2) For P. jinfo, the conservation of high-altitude habitats is of utmost importance, along with the reduction in disturbances caused by roads to maintain the species’ ecological niche. (3) For T. wenxianensis, the protection of aquatic habitats is crucial. Additionally, efforts to mitigate the impacts of road construction and enhance public awareness are essential for the preservation of this species and the connectivity of its habitats. Full article

(This article belongs to the Special Issue Adaptive Responses of Vertebrates to Climate Change)

► Show Figures

Figure 1

19 pages, 9714 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Exploration of Toxins from a Marine Annelid: An Analysis of Phyllotoxins and Accompanying Bioactives

by Ana P. Rodrigo, Inês Moutinho Cabral, António Alexandre and Pedro M. Costa

Animals 2024, 14(4), 635; https://doi.org/10.3390/ani14040635 - 16 Feb 2024

Cited by 1 | Viewed by 3184

Abstract

Proteinaceous toxins are peptides or proteins that hold great biotechnological value, evidenced by their ecological role, whether as defense or predation mechanisms. Bioprospecting using bioinformatics and omics may render screening for novel bioactives more expeditious, especially considering the immense diversity of toxin-secreting marine [...] Read more.

Proteinaceous toxins are peptides or proteins that hold great biotechnological value, evidenced by their ecological role, whether as defense or predation mechanisms. Bioprospecting using bioinformatics and omics may render screening for novel bioactives more expeditious, especially considering the immense diversity of toxin-secreting marine organisms. Eulalia sp. (Annelida: Phyllodocidae), a toxin bearing marine annelid, was recently shown to secrete cysteine-rich protein (Crisp) toxins (hitherto referred to as ‘phyllotoxins’) that can immobilize its prey. By analyzing and validating transcriptomic data, we narrowed the list of isolated full coding sequences of transcripts of the most abundant toxins or accompanying bioactives secreted by the species (the phyllotoxin Crisp, hyaluronidase, serine protease, and peptidases M12A, M13, and M12B). Through homology matching with human proteins, the biotechnological potential of the marine annelid’s toxins and related proteins was tentatively associated with coagulative and anti-inflammatory responses for the peptidases PepM12A, SePr, PepM12B, and PepM13, and with the neurotoxic activity of Crisp, and finally, hyaluronidase was inferred to bear properties of an permeabilizing agent. The in silico analysis succeeded by validation by PCR and Sanger sequencing enabled us to retrieve cDNAs can may be used for the heterologous expression of these toxins. Full article

(This article belongs to the Special Issue Marine Invertebrates: From Ecological Traits to Biotechnological Clues)

► Show Figures

Figure 1

23 pages, 2057 KiB

Open AccessEditor’s ChoiceArticle

Alternatives to Carbon Dioxide in Two Phases for the Improvement of Broiler Chickens’ Welfare during Stunning

by Daniel Santiago Rucinque, Antonio Velarde, Aida Xercavins, Aranzazu Varvaró-Porter, Troy John Gibson, Virginie Michel and Alexandra Contreras-Jodar

Animals 2024, 14(3), 486; https://doi.org/10.3390/ani14030486 - 1 Feb 2024

Viewed by 3197

Abstract

This study evaluated the exposure to gas mixtures of carbon dioxide (CO₂) associated with nitrogen (N₂) as alternatives to CO₂ in two phases to improve the welfare of broiler chickens at slaughter. Broilers were exposed to one of [...] Read more.

This study evaluated the exposure to gas mixtures of carbon dioxide (CO₂) associated with nitrogen (N₂) as alternatives to CO₂ in two phases to improve the welfare of broiler chickens at slaughter. Broilers were exposed to one of three treatments: 40C90C (1st phase: <40% CO₂ for 2 min; 2nd phase: >90% CO₂ and <2% O₂ for 2 min, n = 92), 40C60N (40% CO₂, 60% N₂, and <2% O₂ for 4 min, n = 79), or 20C80N (20% CO₂, 80% N₂, and <2% O₂ for 4 min, n = 72). Brain activity (EEG) was assessed to determine the onset of loss of consciousness (LOC) and death. Behavioural assessment allowed for characterisation of an aversive response to the treatments and confirmed loss of posture (LOP) and motionlessness as behavioural proxies of LOC and brain death in 40C60N and 20N80C. However, the lack of quality of the EEG traces obtained in 40C90C did not allow us to determine the onset of LOC and brain death for this treatment. The onset of LOC in 40C60N was found at 19 s [14–30 s] and in 20C80N at 21 s [16–37 s], whereas a LOP was seen at 53 s [26–156 s] in 40C90C. Birds showed brain death in 40C60N at 64 s [43–108 s] and in 20C80N at 70 s [45–88 s]), while they became motionless in 40C90C at 177 s [89–212 s]. The 40C90C birds not only experienced more events of aversive behaviours related to mucosal irritation, dyspnoea, and breathlessness during induction to unconsciousness but were at risk of remaining conscious when the CO₂ concentration was increased in the 2nd phase (known to cause severe pain). From an animal welfare point of view, 40C60N proved to be the least aversive of the three treatments tested, followed by 20C80N and 40C90C. Full article

(This article belongs to the Special Issue Animal Stress and Welfare During Transport and Slaughtering)

► Show Figures

Figure 1

11 pages, 250 KiB

Open AccessEditor’s ChoiceArticle

Effects of Dietary Crude Protein Level of Concentrate Mix on Growth Performance, Rumen Characteristics, Blood Metabolites, and Methane Emissions in Fattening Hanwoo Steers

by Joonpyo Oh, Hyunjin Cho, Sinyong Jeong, Kyewon Kang, Mingyung Lee, Seoyoung Jeon, Hamin Kang and Seongwon Seo

Animals 2024, 14(3), 469; https://doi.org/10.3390/ani14030469 - 31 Jan 2024

Cited by 3 | Viewed by 2145

Abstract

This study aimed to investigate the effect of varying levels of dietary crude protein (CP) on growth performance, rumen characteristics, blood metabolites, and methane emissions in fattening Hanwoo steers. Twenty-four steers, weighing 504 ± 33.0 kg (16 months old), were assigned to four [...] Read more.

This study aimed to investigate the effect of varying levels of dietary crude protein (CP) on growth performance, rumen characteristics, blood metabolites, and methane emissions in fattening Hanwoo steers. Twenty-four steers, weighing 504 ± 33.0 kg (16 months old), were assigned to four dietary treatments with different CP concentrations (15, 18, 19, and 21% of CP on a dry matter (DM) basis). A linear increasing trend in the average daily gain (ADG) was observed (p = 0.066). With increased dietary CP levels, the rumen ammonia concentration significantly increased (p < 0.001), while the propionate proportion linearly decreased (p = 0.004) and the proportions of butyrate and valerate linearly increased (p ≤ 0.003). The blood urea exhibited a linear increase (p < 0.001), whereas the blood non-esterified fatty acids and cholesterol showed a linear decrease (p ≤ 0.003) with increasing dietary CP. The methane concentration from eructation per intake (ppm/kg), forage neutral detergent fiber (NDF) intake, total NDF intake, and ADG exhibited linear decreases (p ≤ 0.014) across the treatments. In conclusion, increasing the dietary CP up to 21% in concentrates demonstrated a tendency to linearly increase the ADG and significantly decrease the propionate while increasing the butyrate. The methane concentration from eructation exhibited a tendency to linearly decrease with increasing dietary CP. Full article

(This article belongs to the Section Animal Nutrition)

44 pages, 4601 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Impacts of Puppy Early Life Experiences, Puppy-Purchasing Practices, and Owner Characteristics on Owner-Reported Problem Behaviours in a UK Pandemic Puppies Cohort at 21 Months of Age

by Claire L. Brand, Dan G. O’Neill, Zoe Belshaw, Fiona C. Dale, Bree L. Merritt, Kathryn N. Clover, Mi-Xue Michelle Tay, Camilla L. Pegram and Rowena M. A. Packer

Animals 2024, 14(2), 336; https://doi.org/10.3390/ani14020336 - 22 Jan 2024

Cited by 11 | Viewed by 8634

Abstract

Problem behaviours are a leading cause of relinquishment and euthanasia of young dogs. Previous research has identified associations between owner-reported problem behaviours and risk factors, including how dogs were acquired as puppies, early socialisation experiences, and owners’ experience levels. Puppies acquired during the [...] Read more.

Problem behaviours are a leading cause of relinquishment and euthanasia of young dogs. Previous research has identified associations between owner-reported problem behaviours and risk factors, including how dogs were acquired as puppies, early socialisation experiences, and owners’ experience levels. Puppies acquired during the 2020 phase of the UK COVID-19 pandemic (“Pandemic Puppies”) were more likely to be exposed to many of these risk factors compared to puppies acquired in 2019; however, consequences for their adult behaviour are, as yet, unexplored. This study aimed to investigate the impact of these early-life and provenance-based risk factors, in addition to owner management strategies (including training methods) into early adulthood, upon adult dog behaviour aged 21 months. An online longitudinal cohort study of n = 985 Pandemic Puppies was conducted, recruited from a cohort of n = 4369 puppies originally surveyed in November–December 2020, which included data on how and why they were acquired and their socialisation/habituation experiences <16 weeks of age. Nearly all owners (96.7%) reported that their dog had exhibited at least one problem behaviour up to 21 months of age (median: 5; IQR: 3–7). Almost one third of dogs (30.9%) were reported to have displayed separation-related behaviours. Multivariable linear regression modelling revealed that owners reporting more problem behaviours were more likely to use multiple aversive training techniques (e.g., physical punishment), the use of which was notably high in this population (82.3%) compared to previous studies. Attendance at online puppy classes was the sole protective factor against owner use of aversive training methods. Almost one third of owners had underestimated how hard training their dog would be; this view was more likely among first-time owners. Urgent efforts are required to support this vulnerable population of dogs, including providing owner education regarding humane training techniques, to improve canine emotional health and avoid future relinquishment and/or behavioural euthanasia. Full article

(This article belongs to the Section Companion Animals)

► Show Figures

Figure 1

12 pages, 1382 KiB

Open AccessEditor’s ChoiceArticle

An Influx of Non-Native Bird Species into the Natural Environment Owing to the Accidental Release of Pet Birds in Japan

by Sumiko Nishida and Wataru Kitamura

Animals 2024, 14(2), 221; https://doi.org/10.3390/ani14020221 - 10 Jan 2024

Cited by 4 | Viewed by 3957

Abstract

The escape of pet birds into the wild raises concerns about the introduction of invasive avian species. This study investigated the impact of escaped pet birds on the introduction of non-native species in Japan. Data sourced from four lost-and-found pet websites between January [...] Read more.

The escape of pet birds into the wild raises concerns about the introduction of invasive avian species. This study investigated the impact of escaped pet birds on the introduction of non-native species in Japan. Data sourced from four lost-and-found pet websites between January 2018 and December 2021 revealed 12,125 recorded escapes exhibiting both daily occurrences and seasonal fluctuations. Statistical modeling identified the monthly average temperature (positively correlated) and maximum electricity demand (negatively correlated) as influential factors. Text analysis revealed “window” and “open” as frequently cited reasons for escapes. Budgerigars (Melopsittacus undulatus) and Cockatiels (Nymphicus hollandicus) accounted for 76% of the total escape, suggesting a low perceived risk of establishment in nonnative environments. Interestingly, two globally established invasive species, the Rose-ringed Parakeet (Psittacula krameri) and Monk Parakeet (Myiopsitta monachus), were among the escaped birds. While the Rose-ringed Parakeet is locally naturalized in Tokyo and its adjacent prefectures, the Monk Parakeet failed to establish itself in Japan. Despite the limited number of escaped Monk Parakeets, ongoing efforts are crucial for preventing the potential re-establishment of species with such capabilities. Full article

(This article belongs to the Special Issue Invasive Birds: New Advances and Perspectives)

► Show Figures

Figure 1

14 pages, 257 KiB

Open AccessFeature PaperEditor’s ChoiceArticle

Effect of a Dietary Essential Oil Blend in Dairy Cows during the Dry and Transition Period on Blood and Metabolic Parameters of Dams and Their Calves

by Cangir Uyarlar, Abdur Rahman, Eyup Eren Gultepe, Ibrahim Sadi Cetingul and Ismail Bayram

Animals 2024, 14(1), 150; https://doi.org/10.3390/ani14010150 - 2 Jan 2024

Cited by 5 | Viewed by 2844

Abstract

Cows face severe challenges of immunosuppression and negative energy balance during transition periods. The current study was designed to investigate the effects of essential oil blend supplementation in dairy cow’s ration during dry periods on the health of the cow and calf. In [...] Read more.

Cows face severe challenges of immunosuppression and negative energy balance during transition periods. The current study was designed to investigate the effects of essential oil blend supplementation in dairy cow’s ration during dry periods on the health of the cow and calf. In the current study, 45 dry Holstein dairy cows were divided into three groups, each consisting of 15 animals. The control group was without any supplementation; the positive control group was only injected with 0.2 mg/kg levamisole (intramuscular) at 2 months before parturition and 1 month before parturition; and the treatment group was supplemented with 3 g/day for each cow essential oil blend mixed in total mixed ration (TMR). A mixed vaccine against E. coli, Rotavirus, and Coronavirus was administered to all cows before 42 days and after 21 days of calving. The day of the calving of the animal was accepted as day 0. Blood samples were collected from the coccygeal vein of all cows at −60, −45, −30, −15, −7, 0, 1 and 2 days, and the colostrum samples were taken on days 0, 1, and 2. Blood samples were also collected from the jugular vein (V. Jugularis) of the newborn calves on days 0, 1, and 2. The results of the hematological parameters revealed no difference in the total red blood cell count, hemoglobin amount, hematocrit, MCV, MCH, MCHC, RDW, PLT, MPV, and PCT values in both prenatal and postnatal blood of the cows (p > 0.05). In terms of immunological parameters, the total IgG level was significantly higher in the experimental group on the 7th day before birth compared to the other two groups, while the total leukocyte count, lymphocyte count, monocyte count and granulocyte counts were found to be lower after birth (p < 0005). Blood NEFA and BHBA levels were also lower in the experimental group compared to the other two groups (p < 0.005), and the blood glucose level was not different (p > 0.05). In calves, no difference was found between the groups in any of the parameters examined (p > 0.05). It is concluded that the dietary addition of an essential oil blend during the transition period enhanced the immune status and energy metabolism of cows without any effect on the health status of newborn calves. Full article

(This article belongs to the Section Animal Physiology)

12 pages, 3766 KiB

Open AccessEditor’s ChoiceArticle

Cultivable Gut Microbiota in Synanthropic Bats: Shifts of Its Composition and Diversity Associated with Hibernation

by Igor V. Popov, Iraida S. Berezinskaia, Ilia V. Popov, Irina B. Martiusheva, Elizaveta V. Tkacheva, Vladislav E. Gorobets, Iuliia A. Tikhmeneva, Anna V. Aleshukina, Tatiana I. Tverdokhlebova, Michael L. Chikindas, Koen Venema and Alexey M. Ermakov

Animals 2023, 13(23), 3658; https://doi.org/10.3390/ani13233658 - 26 Nov 2023

Cited by 8 | Viewed by 2763

Abstract

The role of bats in the global microbial ecology no doubt is significant due to their unique immune responses, ability to fly, and long lifespan, all contributing to pathogen spread. Some of these animals hibernate during winter, which results in the altering of [...] Read more.

The role of bats in the global microbial ecology no doubt is significant due to their unique immune responses, ability to fly, and long lifespan, all contributing to pathogen spread. Some of these animals hibernate during winter, which results in the altering of their physiology. However, gut microbiota shifts during hibernation is little studied. In this research, we studied cultivable gut microbiota composition and diversity of Nyctalus noctula before, during, and after hibernation in a bat rehabilitation center. Gut microorganisms were isolated on a broad spectrum of culture media, counted, and identified with mass spectrometry. Linear modeling was used to investigate associations between microorganism abundance and N. noctula physiological status, and alpha- and beta-diversity indexes were used to explore diversity changes. As a result, most notable changes were observed in Serratia liquefaciens, Hafnia alvei, Staphylococcus sciuri, and Staphylococcus xylosus, which were significantly more highly abundant in hibernating bats, while Citrobacter freundii, Klebsiella oxytoca, Providencia rettgeri, Citrobacter braakii, and Pedicoccus pentosaceus were more abundant in active bats before hibernation. The alpha-diversity was the lowest in hibernating bats, while the beta-diversity differed significantly among all studied periods. Overall, this study shows that hibernation contributes to changes in bat cultivable gut microbiota composition and diversity. Full article

(This article belongs to the Special Issue Bats of Europe: Current Research on Diversity, Ecology and Conservation Needs)

► Show Figures

Figure 1

18 pages, 2195 KiB

Open AccessEditor’s ChoiceArticle

Understanding the Factors Influencing Cat Containment: Identifying Opportunities for Behaviour Change

by Gemma C. Ma and Lynette J. McLeod

Animals 2023, 13(10), 1630; https://doi.org/10.3390/ani13101630 - 12 May 2023

Cited by 11 | Viewed by 3861

Abstract

There are over 5 million pet cats in Australia managed on a spectrum from fully indoors to completely outdoor free roaming. Roaming cats threaten biodiversity, can create a nuisance and are at risk of accidents and injury. Hence, there is substantial interest in [...] Read more.

There are over 5 million pet cats in Australia managed on a spectrum from fully indoors to completely outdoor free roaming. Roaming cats threaten biodiversity, can create a nuisance and are at risk of accidents and injury. Hence, there is substantial interest in behaviour change interventions to increase cat containment. An online questionnaire collected information on cat owner demographics, the number of cats owned, current containment behaviours and an agreement with 15 capability, opportunity and motivation (COM) items. Responses were received from 4482 cat owners. More than half (65%) indicated that they currently keep their cat(s) fully contained. Another 24% practiced a night curfew. Owners’ psychological capability had the greatest influence on containment behaviour. Motivation (community- and cat welfare-framed), living in an apartment and renting were also associated with a greater likelihood of containment. Cat owners not currently containing their cats could be divided into six profiles who differed on agreement with COM themes, age, future intentions, current behaviour, location and gender. Understanding differences between cat owner segments can assist with designing behaviour change interventions. Increasing cat owners’ psychological capability to contain their cats and encouraging the adoption of a night curfew as a first step towards 24 h containment are recommended. Full article

(This article belongs to the Section Companion Animals)

► Show Figures

Figure 1

12 pages, 872 KiB

Open AccessEditor’s ChoiceArticle

Multimodal Communication in the Human–Cat Relationship: A Pilot Study

by Charlotte de Mouzon and Gérard Leboucher

Animals 2023, 13(9), 1528; https://doi.org/10.3390/ani13091528 - 3 May 2023

Cited by 7 | Viewed by 21102

Abstract

Across all species, communication implies that an emitter sends signals to a receiver, through one or more channels. Cats can integrate visual and auditory signals sent by humans and modulate their behaviour according to the valence of the emotion perceived. However, the specific [...] Read more.

Across all species, communication implies that an emitter sends signals to a receiver, through one or more channels. Cats can integrate visual and auditory signals sent by humans and modulate their behaviour according to the valence of the emotion perceived. However, the specific patterns and channels governing cat-to-human communication are poorly understood. This study addresses whether, in an extraspecific interaction, cats are sensitive to the communication channel used by their human interlocutor. We examined three types of interactions—vocal, visual, and bimodal—by coding video clips of 12 cats living in cat cafés. In a fourth (control) condition, the human interlocutor refrained from emitting any communication signal. We found that the modality of communication had a significant effect on the latency in the time taken for cats to approach the human experimenter. Cats interacted significantly faster to visual and bimodal communication compared to the “no communication” pattern, as well as to vocal communication. In addition, communication modality had a significant effect on tail-wagging behaviour. Cats displayed significantly more tail wagging when the experimenter engaged in no communication (control condition) compared to visual and bimodal communication modes, indicating that they were less comfortable in this control condition. Cats also displayed more tail wagging in response to vocal communication compared to the bimodal communication. Overall, our data suggest that cats display a marked preference for both visual and bimodal cues addressed by non-familiar humans compared to vocal cues only. Results arising from the present study may serve as a basis for practical recommendations to navigate the codes of human–cat interactions. Full article

(This article belongs to the Section Companion Animals)

► Show Figures

Figure 1

23 pages, 5672 KiB

Open AccessEditor’s ChoiceArticle

Taurine Supplementation to Plant-Based Diets Improves Lipid Metabolism in Senegalese Sole

by Cláudia Aragão, Rita Teodósio, Rita Colen, Nadège Richard, Ivar Rønnestad, Jorge Dias, Luís E. C. Conceição and Laura Ribeiro

Animals 2023, 13(9), 1501; https://doi.org/10.3390/ani13091501 - 28 Apr 2023

Cited by 7 | Viewed by 4802

Abstract

Taurine is a sulphur-containing amino acid with important physiological roles and a key compound for the synthesis of bile salts, which are essential for the emulsion and absorption of dietary lipids. This study aimed to evaluate the effects of taurine supplementation to low-fishmeal [...] Read more.

Taurine is a sulphur-containing amino acid with important physiological roles and a key compound for the synthesis of bile salts, which are essential for the emulsion and absorption of dietary lipids. This study aimed to evaluate the effects of taurine supplementation to low-fishmeal diets on the metabolism of taurine, bile acids, and lipids of Senegalese sole. A fishmeal (FM) and a plant-protein-based (PP0) diet were formulated, and the latter was supplemented with taurine at 0.5 and 1.5% (diets PP0.5 and PP1.5). Diets were assigned to triplicate tanks containing 35 fish (initial weight ~14 g) for 6 weeks. Fish from the PP0 treatment presented lower taurine and bile-acid concentrations compared with the FM treatment, and a downregulation of cyp7a1 and abcb11 was observed. Triolein catabolism decreased in PP0-fed fish, resulting in increased hepatic fat content and plasma triglycerides, while no effects on plasma cholesterol were observed. Taurine supplementation to plant-based diets resulted in a higher taurine accumulation in fish tissues, increased bile-acid concentration, and upregulation of cyp7a1 and abcb11. Hepatic fat content and plasma triglycerides decreased with increasing dietary taurine supplementation. Taurine supplementation mitigated part of the negative effects of plant-based diets, leading to better lipid utilisation. Full article

(This article belongs to the Special Issue Amino Acid Supplementation in Fish Nutrition and Welfare)

► Show Figures

Figure 1

17 pages, 2294 KiB

Open AccessEditor’s ChoiceArticle

Exploring the Effect of Functional Diets Containing Phytobiotic Compounds in Whiteleg Shrimp Health: Resistance to Acute Hepatopancreatic Necrotic Disease Caused by Vibrio parahaemolyticus

by Carla Hernández-Cabanyero, Esther Carrascosa, Silvia Jiménez and Belén Fouz

Animals 2023, 13(8), 1354; https://doi.org/10.3390/ani13081354 - 15 Apr 2023

Cited by 11 | Viewed by 5082

Abstract

Acute hepatopancreatic necrosis (AHPND) is an emerging severe disease caused by strains of Vibrio parahaemolyticus (Vp_AHPND) in whiteleg shrimp (Litopenaeus vannamei). Mitigating its negative impact, and at the same time minimizing antibiotics treatments, is the major challenge in [...] Read more.

Acute hepatopancreatic necrosis (AHPND) is an emerging severe disease caused by strains of Vibrio parahaemolyticus (Vp_AHPND) in whiteleg shrimp (Litopenaeus vannamei). Mitigating its negative impact, and at the same time minimizing antibiotics treatments, is the major challenge in shrimp aquaculture. A sustainable strategy could be to include immunostimulants in diet. Phytobiotics, harmless plant extracts with immunostimulatory and biocidal activities, are promising candidates. In this study, we evaluated the effectiveness of two diets (E and F) supplemented with phytobiotics (functional diets) in terms of protecting shrimp against AHPND. For this purpose, groups of animals were fed functional or control diets for 4 and 5 weeks and, subsequently, they were challenged with Vp_AHPND by immersion. We compared the mortality in infected groups and estimated the percentage of carriers by using a specific qPCR in hepatopancreas tissue. The results showed that mortality was significantly lower in the group fed functional diet E and, after a 5-week feeding schedule. This group also showed the lowest percentage of carriers. The pathological effects were also reduced with diet F. Thus, feeding shrimp with phytobiotic-enriched diets in critical periods will be highly beneficial because it increases the host’s resistance to AHPND pathology. Full article

(This article belongs to the Special Issue Functional Feeds to Improve Shrimp and Fish Aquaculture)

► Show Figures

Figure 1

16 pages, 4922 KiB

Open AccessEditor’s ChoiceArticle

Red Sea Bream Iridovirus Kinetics, Tissue Tropism, and Interspecies Horizontal Transmission in Flathead Grey Mullets (Mugil cephalus)

by Kyung-Ho Kim, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Mun-Gyeong Kwon, Jae-Ok Kim and Chan-Il Park

Animals 2023, 13(8), 1341; https://doi.org/10.3390/ani13081341 - 13 Apr 2023

Cited by 6 | Viewed by 3131

Abstract

Red sea bream iridovirus (RSIV) causes significant economic losses in the aquaculture industry. We analyzed the pathogenicity of RSIV in flathead grey mullets (Mugil cephalus), the correlation of histopathological lesions, and interspecies horizontal transmission, through immersion infection and cohabitation challenges. Flathead [...] Read more.

Red sea bream iridovirus (RSIV) causes significant economic losses in the aquaculture industry. We analyzed the pathogenicity of RSIV in flathead grey mullets (Mugil cephalus), the correlation of histopathological lesions, and interspecies horizontal transmission, through immersion infection and cohabitation challenges. Flathead grey mullets, which were challenged by immersion infection, exhibited mortality at 14 and 24 days after RSIV exposure. Viral shedding in seawater peaked 2–3 days before or after the observed mortality. Specific lesions of RSIV were observed in the spleen and kidney, and the correlation between histopathological grade and viral load was the highest in the spleen. In a cohabitation challenge, flathead grey mullets were the donors, and healthy rock bream, red sea bream, and flathead grey mullets were the recipients. Viral shedding in seawater was the highest in flathead grey mullet and rock bream at 25 °C, with 10^6.0 RSIV copies L/g at 14 dpi. No mortality was observed in any group challenged at 15 °C, and no RSIV was detected in seawater after 30 dpi. The virus shed from RSIV-infected flathead grey mullets caused horizontal transmission through seawater. These findings suggest that rapid decision-making is warranted when managing disease in fish farms. Full article

(This article belongs to the Section Aquatic Animals)

► Show Figures

Figure 1

14 pages, 1240 KiB

Open AccessEditor’s ChoiceArticle

Acoustic Monitoring Confirms Significant Poaching Pressure of European Turtle Doves (Streptopelia turtur) during Spring Migration across the Ionian Islands, Greece

by Christos Astaras, Zoi-Antigoni Sideri-Manoka, Manolia Vougioukalou, Despina Migli, Ioakim Vasiliadis, Sotirios Sidiropoulos, Christos Barboutis, Aris Manolopoulos, Michalis Vafeiadis and Savas Kazantzidis

Animals 2023, 13(4), 687; https://doi.org/10.3390/ani13040687 - 16 Feb 2023

Cited by 3 | Viewed by 4450

Abstract

The European turtle dove (Streptopelia turtur) is an Afro-Palearctic migrant whose populations have declined by 79% from 1980 to 2014. In 2018, the International Single Species Action Plan for the Turtle Dove (ISSAP) was developed with the goal of enabling, by [...] Read more.

The European turtle dove (Streptopelia turtur) is an Afro-Palearctic migrant whose populations have declined by 79% from 1980 to 2014. In 2018, the International Single Species Action Plan for the Turtle Dove (ISSAP) was developed with the goal of enabling, by 2028, an increase in turtle dove numbers along each of the three migration flyways (western, central, eastern). To achieve this, the illegal killing of turtle doves, a critical threat to the species, has to be eradicated. The Ionian Islands off the west coast of Greece lie on the eastern flyway and are considered a major turtle dove poaching hot-spot during spring migration. Quantifying wildlife crime, however, is challenging. In the absence of a reliable protocol for monitoring spring poaching levels, the agencies tasked with tackling the problem have no means of assessing the effectiveness of the anti-poaching measures and adapting them if required. Using passive acoustic monitoring (PAM) methods, we recorded gun hunting intensity at known turtle dove poaching sites during the 2019–2022 spring migrations (2–10 sites/season) with unprecedented spatial and temporal resolution. Based on published gunshot to killed/injured bird ratio for similar species (corroborated with discussions with local hunters) and an estimate of the proportion of hunting sites monitored by our PAM grid (using gunshot detection range estimates from control gunshots), we estimated that in 2021, up to 57,095 turtle doves were killed or injured across five Ionian Islands (Zakynthos, Paxi, Antipaxi, Othoni, and Mathraki). The 2022 estimate was almost half, but it is unclear as to whether the change is due to a decline in poachers or turtle doves. We propose ways of improving confidence in future estimates, and call for a temporary moratorium of autumn turtle dove hunting in Greece—as per ISSAP recommendation—until spring poaching is eradicated and the eastern flyway population shows signs of a full recovery. Finally, we hope our findings will pave the way for the development of PAM grids at turtle dove poaching hot-spots across all migration flyways, contributing to the global conservation of the species. Full article

(This article belongs to the Section Birds)

► Show Figures

Figure 1

10 pages, 291 KiB

Open AccessEditor’s ChoiceArticle

Dental Disease in Rabbits (Oryctolagus cuniculus) and Its Risk Factors—A Private Practice Study in the Metropolitan Region of Chile

by Tamara Palma-Medel, Daniela Marcone and Raúl Alegría-Morán

Animals 2023, 13(4), 676; https://doi.org/10.3390/ani13040676 - 15 Feb 2023

Cited by 17 | Viewed by 3918

Abstract

Rabbits (Oryctolagus cuniculus) have elodont dentition, a characteristic that predisposes them to the development of Acquired Dental Disease (ADD), which is a multifactorial disease. The aim of this study was to assess the risk factors for ADD in domestic pet rabbits. [...] Read more.

Rabbits (Oryctolagus cuniculus) have elodont dentition, a characteristic that predisposes them to the development of Acquired Dental Disease (ADD), which is a multifactorial disease. The aim of this study was to assess the risk factors for ADD in domestic pet rabbits. To do this, a retrospective analysis of 1420 rabbits treated at a private practice during 2018–2021 was performed. For this, several variables were retrieved from clinical records, in addition to signology at the time of diagnosis. ADD was found on 25.4% of rabbits, mostly on their cheek teeth. In addition, age (OR = 1.029; 95% CI = 1.023–1.035; p < 0.001) and sex (male) (OR = 1.591; 95% CI = 1.226–2.064; p < 0.001) were found to be significant risk factors for ADD. In contrast, a free lifestyle (OR = 0.565; 95% CI = 0.362–0.882; p = 0.012) and consuming hay in the diet (OR = 0.323; 95% CI = 0.220–0.473; p < 0.001) were protective factors. In conclusion, ADD has a high prevalence and is usually underdiagnosed, highlighting the need for an exhaustive evaluation of patients during the clinical examination. This study improves our knowledge of ADD and its prevention. Full article

(This article belongs to the Section Veterinary Clinical Studies)

9 pages, 3139 KiB

Open AccessEditor’s ChoiceCommunication

Assessment of Ventral Tail Base Surface Temperature for the Early Detection of Japanese Black Calves with Fever

by Yosuke Sasaki, Yoshihiro Iki, Tomoaki Anan, Jun Hayashi and Mizuho Uematsu

Animals 2023, 13(3), 469; https://doi.org/10.3390/ani13030469 - 29 Jan 2023

Cited by 2 | Viewed by 2532

Abstract

The objective in the present study was to assess the ventral tail base surface temperature (ST) for the early detection of Japanese Black calves with fever. This study collected data from a backgrounding operation in Miyazaki, Japan, that included 153 calves aged 3–4 [...] Read more.

The objective in the present study was to assess the ventral tail base surface temperature (ST) for the early detection of Japanese Black calves with fever. This study collected data from a backgrounding operation in Miyazaki, Japan, that included 153 calves aged 3–4 months. A wearable wireless ST sensor was attached to the surface of the ventral tail base of each calf at its introduction to the farm. The ventral tail base ST was measured every 10 min for one month. The present study conducted an experiment to detect calves with fever using the estimated residual ST (rST), calculated as the estimated rST minus the mean estimated rST for the same time on the previous 3 days, which was obtained using machine learning algorithms. Fever was defined as an increase of ≥1.0 °C for the estimated rST of a calf for 4 consecutive hours. The machine learning algorithm that applied was a random forest, and 15 features were included. The variable importance scores that represented the most important predictors for the detection of calves with fever were the minimum and maximum values during the last 3 h and the difference between the current value and 24- and 48-h minimum. For this prediction model, accuracy, precision, and sensitivity were 98.8%, 72.1%, and 88.1%, respectively. The present study indicated that the early detection of calves with fever can be predicted by monitoring the ventral tail base ST using a wearable wireless sensor. Full article

(This article belongs to the Special Issue Artificial Intelligence (AI) Applied to Animal Health and Welfare)

► Show Figures

Figure 1

12 pages, 2371 KiB

Open AccessEditor’s ChoiceArticle

Comparative Metabolome Analyses of Ivermectin-Resistant and -Susceptible Strains of Haemonchus contortus

by Waresi Tuersong, Xin Liu, Yifan Wang, Simin Wu, Peixi Qin, Shengnang Zhu, Feng Liu, Chunqun Wang and Min Hu

Animals 2023, 13(3), 456; https://doi.org/10.3390/ani13030456 - 28 Jan 2023

Cited by 6 | Viewed by 2895

Abstract

Resistance to anthelmintics such as ivermectin (IVM) is currently a major problem in the treatment of Haemonchus contortus, an important parasitic nematode of small ruminants. Although many advances have been made in understanding the IVM resistance mechanism, its exact mechanism remains unclear [...] Read more.

Resistance to anthelmintics such as ivermectin (IVM) is currently a major problem in the treatment of Haemonchus contortus, an important parasitic nematode of small ruminants. Although many advances have been made in understanding the IVM resistance mechanism, its exact mechanism remains unclear for H. contortus. Therefore, understanding the resistance mechanism becomes increasingly important for controlling haemonchosis. Recent research showed that the metabolic state of bacteria influences their susceptibility to antibiotics. However, little information is available on the roles of metabolites and metabolic pathways in IVM resistance of H. contortus. In this study, comparative analyses of the metabolomics of IVM-susceptible and -resistant adult H. contortus worms were carried out to explore the role of H. contortus metabolism in IVM resistance. In total, 705 metabolites belonging to 42 categories were detected, and 86 differential metabolites (17 upregulated and 69 downregulated) were identified in the IVM-resistant strain compared to the susceptible one. A KEGG pathway analysis showed that these 86 differential metabolites were enriched in 42 pathways that mainly included purine metabolism; the biosynthesis of amino acids; glycine, serine, and threonine metabolism; and cysteine and methionine metabolism. These results showed that amino acid metabolism may be mediated by the uptake of IVM and related with IVM resistance in H. contortus. This study contributes to our understanding of the mechanisms of IVM resistance and may provide effective approaches to manage infection by resistant strains of H. contortus. Full article

(This article belongs to the Special Issue Advances in the Diagnosis of Parasitic Infections in Animals)

► Show Figures

Figure 1

11 pages, 1952 KiB

Open AccessEditor’s ChoiceArticle

Conservation of Major Satellite DNAs in Snake Heterochromatin

by Artem Lisachov, Alexander Rumyantsev, Dmitry Prokopov, Malcolm Ferguson-Smith and Vladimir Trifonov

Animals 2023, 13(3), 334; https://doi.org/10.3390/ani13030334 - 17 Jan 2023

Cited by 11 | Viewed by 3012

Abstract

Repetitive DNA sequences constitute a sizeable portion of animal genomes, and tandemly organized satellite DNAs are a major part of them. They are usually located in constitutive heterochromatin clusters in or near the centromeres or telomeres, and less frequently in the interstitial parts [...] Read more.

Repetitive DNA sequences constitute a sizeable portion of animal genomes, and tandemly organized satellite DNAs are a major part of them. They are usually located in constitutive heterochromatin clusters in or near the centromeres or telomeres, and less frequently in the interstitial parts of chromosome arms. They are also frequently accumulated in sex chromosomes. The function of these clusters is to sustain the architecture of the chromosomes and the nucleus, and to regulate chromosome behavior during mitosis and meiosis. The study of satellite DNA diversity is important for understanding sex chromosome evolution, interspecific hybridization, and speciation. In this work, we identified four satellite DNA families in the genomes of two snakes from different families: Daboia russelii (Viperidae) and Pantherophis guttatus (Colubridae) and determine their chromosomal localization. We found that one family is localized in the centromeres of both species, whereas the others form clusters in certain chromosomes or subsets of chromosomes. BLAST with snake genome assemblies showed the conservation of such clusters, as well as a subtle presence of the satellites in the interspersed manner outside the clusters. Overall, our results show high conservation of satellite DNA in snakes and confirm the “library” model of satellite DNA evolution. Full article

(This article belongs to the Special Issue Reptile Evolution and Genetics - Special Issue Dedicated to the Memory of Prof. Teresa Capriglione)

► Show Figures

Figure 1

22 pages, 1438 KiB

Open AccessEditor’s ChoiceArticle

Characterization of the Fecal and Mucosa-Associated Microbiota in Dogs with Chronic Inflammatory Enteropathy

by David Díaz-Regañón, Mercedes García-Sancho, Alejandra Villaescusa, Ángel Sainz, Beatriz Agulla, Mariana Reyes-Prieto, Antonio Rodríguez-Bertos and Fernando Rodríguez-Franco

Animals 2023, 13(3), 326; https://doi.org/10.3390/ani13030326 - 17 Jan 2023

Cited by 19 | Viewed by 4171

Abstract

Canine chronic inflammatory enteropathy implicates multifactorial pathogenesis where immunological dysregulation and gut microbiota changes have a central role. Most sequencing-based taxonomic studies have been focused on the fecal microbiota. However, the analysis of these samples does not provide complete information regarding the composition [...] Read more.

Canine chronic inflammatory enteropathy implicates multifactorial pathogenesis where immunological dysregulation and gut microbiota changes have a central role. Most sequencing-based taxonomic studies have been focused on the fecal microbiota. However, the analysis of these samples does not provide complete information regarding the composition of the small intestine affected by this canine disease. Therefore, in this study, we aimed to characterize the intestinal bacterial microbiota in dogs with inflammatory bowel disease (IBD) (n = 34) by means of duodenal biopsies and fecal samples collected at the time of the diagnosis and to compare those to a group of healthy dogs (n = 12) using the 16S ribosomal RNA (16S rRNA) gene-targeted sequencing (Illumina MiSeq platform). Our study showed that IBD dogs presented differences in the fecal bacterial communities when compared with healthy dogs, with a lower relative abundance of Prevotellaceae (p = 0.005), Prevotella (p = 0.002), and Prevotellaceae Ga6A1 group (0.006); Erysipelotrichales (p = 0.019), Candidatus Stoquefichus (p < 0.001), Erysipelotrichaceae (p = 0.011), and Allobaculum (p = 0.003); Lachnospiraceae NK4A136 group (p = 0.015), Sellimonas (p = 0.042), Oscillospirales (p = 0.037), Oscillospiraceae UCG–005 (p < 0.001), Faecalibacterium (p = 0.028), and Fournierella (p = 0.034); Acidaminococcales, Acidaminococcaceae, and Phascolarctobacterium (p = 0.001); Aeromonadales (p = 0.026), Succinivibrionaceae (p = 0.037), and Succinivibrio (p = 0.031). On the other hand, a higher relative abundance of Enterococcaceae (Enterococcus; p = 0.003), Streptococcaceae (Streptococcus, p = 0.021), Enterobacterales (p = 0.027), Enterobacteriaceae (p = 0.008), and Escherichia–Shigella (p = 0.011) was detected. Moreover, when evaluating α–diversity, the dogs with IBD showed lower diversity in terms of richness and abundance of species (observed species [p = 0.031] and Shannon index [p = 0.039]). Furthermore, fecal microbiota in dogs with IBD was significantly different from healthy dogs (p = 0.006). However, only a few taxa relative abundance shifts (lower Rubrobacteria, Rubrobacterales, Rubrobacteriaceae, and Rubrobacter [p = 0.002]; Cyanobacteria [p = 0.010], Vampirivibrionia, Obscuribacterales, and Obscuribacteraceae [p = 0.005]; Neisseriaceae [p = 0.004] and Conchiformibius [p = 0.003]) were observed when assessing duodenal-associated microbiota of dogs with IBD. Thus, even if the bowel inflammation mainly affects the small intestine in the IBD-affected dogs of the study, fecal specimens may constitute a better sample due not only to their easy availability but also in terms of searching for bacterial taxa as biomarkers for canine IBD. The use of different diets in the study can also have a partial influence on the microbiota composition. Future studies encompassing multi-omics approaches should evaluate the functionality in both levels to unravel the pathophysiology of canine IBD. Full article

(This article belongs to the Special Issue Microbiome and Resistome in Companion Animals and at the Animal–Human Interface)

► Show Figures

Figure 1

13 pages, 684 KiB

Open AccessEditor’s ChoiceArticle

When and Why Cats Are Returned to Shelters

by Vivian Mundschau and Malini Suchak

Animals 2023, 13(2), 243; https://doi.org/10.3390/ani13020243 - 9 Jan 2023

Cited by 6 | Viewed by 5443

Abstract

There is considerable research on why cats are initially relinquished to shelters, but much less attention has been given to returns, despite the significant implications for shelter capacity and cat welfare. Furthermore, the structure of many databases fails to account for cats who [...] Read more.

There is considerable research on why cats are initially relinquished to shelters, but much less attention has been given to returns, despite the significant implications for shelter capacity and cat welfare. Furthermore, the structure of many databases fails to account for cats who are returned beyond 30 days, despite this making up a substantial portion of returns. In the current study, we examined common risk factors and reasons for return in a population of 2642 shelter cats. We found that cats who were older at the time of adoption or had a bite history had an increased risk of return, whereas cats that were in foster care prior to adoption had a decreased risk of return. We divided the returns by the time to return (<30 days: short term, >30 days: long term) to examine whether time to return had an impact. Approximately half the cats were returned in the short term. Cats were more likely to be returned for reasons, such as behavior, unwanted, and other pet in the short term and personal reasons, cost, euthanasia, and stray in the long-term return. Strategies to reduce returns should consider different solutions for short and long returns to maximize effectiveness. Full article

(This article belongs to the Section Companion Animals)

► Show Figures

Graphical abstract

17 pages, 554 KiB

Open AccessEditor’s ChoiceArticle

Radiographic Diagnosis of Hip Laxity in Rottweilers: Interobserver Agreement at Eight- and Twelve-Months of Age

by Masoud Aghapour, Barbara Bockstahler, Sibylle Kneissl, Aldo Vezzoni, Michaela Gumpenberger, Harald Hechinger, Alexander Tichy and Britta Vidoni

Animals 2023, 13(2), 231; https://doi.org/10.3390/ani13020231 - 8 Jan 2023

Cited by 2 | Viewed by 2650

Abstract

Hip laxity is one of the predisposing factors of canine hip dysplasia. The early diagnosis of hip laxity allows veterinarians to prevent the participation of dysplastic dogs in breeding programs, which could lower the disease’s prevalence due to its genetic background. Furthermore, it [...] Read more.

Hip laxity is one of the predisposing factors of canine hip dysplasia. The early diagnosis of hip laxity allows veterinarians to prevent the participation of dysplastic dogs in breeding programs, which could lower the disease’s prevalence due to its genetic background. Furthermore, it allows them to plan preventive/therapeutic procedures for mild/near-normal hips to reduce the symptoms of the disease at older ages. A reliable screening program must be repeatable and reproducible, and intra- and inter-observer studies can help us to determine the best methods. In this study, we aimed to evaluate the radiographic parameters used for the early diagnosis of hip dysplasia in Rottweilers at 8 and 12 months of age with five observers to assess the interobserver agreements. According to our findings, there were high interobserver agreements at both ages for the quantitative values, such as the center edge angle (CEA), dorsal acetabular rim slope (DARS), distraction index (DI), and Norberg angle (NA), whereas we recorded from poor to moderate agreements for the qualitative values, such as the grading of the dorsal acetabular rim (GDAR), grading of the degenerative joint disease (GDJD), location of the center of the femoral head (LCFH), and sclerosis of the cranial acetabular rim (SCAR). Full article

(This article belongs to the Section Veterinary Clinical Studies)

► Show Figures

Figure 1

17 pages, 2070 KiB

Open AccessEditor’s ChoiceArticle

Population Genetic Structure of Anisakis simplex Infecting the European Hake from North East Atlantic Fishing Grounds

by Andrea Ramilo, Helena Rodríguez, Santiago Pascual, Ángel F. González and Elvira Abollo

Animals 2023, 13(2), 197; https://doi.org/10.3390/ani13020197 - 4 Jan 2023

Cited by 10 | Viewed by 3381

Abstract

The European hake, one of the most commercially valuable species in ICES fishing areas, is considered an important neglected source of zoonotic risk by nematode parasites belonging to the genus Anisakis. Merluccius merluccius is, by far, the most important host of Anisakis [...] Read more.

The European hake, one of the most commercially valuable species in ICES fishing areas, is considered an important neglected source of zoonotic risk by nematode parasites belonging to the genus Anisakis. Merluccius merluccius is, by far, the most important host of Anisakis spp. at the European fishing grounds, in terms of demographic infection values, and carries the highest parasite burden. These high parasite population densities within an individual fish host offer a chance to explore new sources of variations for the genetic structure of Anisakis spp. populations. A total of 873 Anisakis spp. third-stage larvae, originally sampled from viscera and muscular sections of hake collected at ten fishing grounds, were primarily identified using ITS rDNA region as molecular marker. After that, we used mtDNA cox2 gene to reveal the high haplotype diversity and the lack of genetic structure for A. simplex. Dominant haplotypes were shared among the different fishing areas and fish sections analyzed. Results indicate a clear connection of A. simplex from European hake along the Northern North Sea to the Portuguese coast, constituting a single genetic population but revealing a certain level of genetic sub-structuring on the Northwest coast of Scotland. This study also provides useful information to advance the understanding of parasite speciation to different fish host tissues or microenvironments. Full article

(This article belongs to the Special Issue Molecular Identification, Taxonomy and Genetic Variation of Parasite in Animals)

► Show Figures

Figure 1

9 pages, 845 KiB

Open AccessEditor’s ChoiceArticle

Investigation of the Efficacy of Pyrantel Pamoate, Mebendazole, Albendazole, and Ivermectin against Baylisascaris schroederi in Captive Giant Pandas

by Yaxian Lu, Linhua Deng, Zhiwei Peng, Mengchao Zhou, Chengdong Wang, Lei Han, Shan Huang, Ming Wei, Rongping Wei, Lihong Tian, Desheng Li and Zhijun Hou

Animals 2023, 13(1), 142; https://doi.org/10.3390/ani13010142 - 29 Dec 2022

Cited by 1 | Viewed by 3805

Abstract

Baylisascaris schroederi is one of the main health risks threatening both wild and captive giant pandas. The administration of anthelmintics is a common method to effectively control B. schroederi infection, but there is a notable risk of anthelmintic resistance (AR) after long-term, constant [...] Read more.

Baylisascaris schroederi is one of the main health risks threatening both wild and captive giant pandas. The administration of anthelmintics is a common method to effectively control B. schroederi infection, but there is a notable risk of anthelmintic resistance (AR) after long-term, constant use of anthelmintics. Four anthelmintics—pyrantel pamoate (PYR), mebendazole (MBZ), albendazole (ABZ), and ivermectin (IVM)—were each administered separately at intervals of 2 months to 22 enrolled giant pandas. The fecal egg count reduction (FECR) proportions were calculated by both the Markov chain Monte Carlo (MCMC) Bayesian mathematical model and the arithmetic mean. AR was assessed based on the criteria recommended by the World Association for the Advancement of Veterinary Parasitology (WAAVP). The estimated prevalence of B. schroederi infection was 34.1%. After treatment with PYR, MBZ, ABZ, and IVM, it was determined that MBZ, ABZ, and IVM were efficacious against B. schroederi, while nematodes were suspected to be resistant to PYR according to the fecal egg count reduction (FECR) proportions. Full article

(This article belongs to the Special Issue Veterinary Parasitology: Epidemiology, Control and Prevention Strategies)

► Show Figures

Figure 1

12 pages, 1354 KiB

Open AccessEditor’s ChoiceArticle

Role of Guinea Pigs (Cavia porcellus) Raised as Livestock in Ecuadorian Andes as Reservoirs of Zoonotic Yeasts

by Lenys Buela, Mercy Cuenca, Jéssica Sarmiento, Diana Peláez, Ana Yolanda Mendoza, Erika Judith Cabrera and Luis Andrés Yarzábal

Animals 2022, 12(24), 3449; https://doi.org/10.3390/ani12243449 - 7 Dec 2022

Cited by 5 | Viewed by 4598

Abstract

Guinea pigs (Cavia porcellus) have been reared for centuries in the Andean region for ceremonial purposes or as the main ingredient of traditional foods. The animals are kept in close proximity of households and interact closely with humans; this also occurs in [...] Read more.

Guinea pigs (Cavia porcellus) have been reared for centuries in the Andean region for ceremonial purposes or as the main ingredient of traditional foods. The animals are kept in close proximity of households and interact closely with humans; this also occurs in western countries, where guinea pigs are considered pets. Even though it is acknowledged that domestic animals carry pathogenic yeasts in their tissues and organs that can cause human diseases, almost nothing is known in the case of guinea pigs. In this work we used traditional microbiological approaches and molecular biology techniques to isolate, identify, and characterize potentially zoonotic yeasts colonizing the nasal duct of guinea pigs raised as livestock in Southern Ecuador (Cañar Province). Our results show that 44% of the 100 animals studied were colonized in their nasal mucosa by at least eleven yeast species, belonging to eight genera: Wickerhamomyces, Diutina, Meyerozyma, Candida, Pichia, Rhodotorula, Galactomyces, and Cryptococcus. Noticeably, several isolates were insensitive toward several antifungal drugs of therapeutic use, including fluconazole, voriconazole, itraconazole, and caspofungin. Together, our results emphasize the threat posed by these potentially zoonotic yeasts to the farmers, their families, the final consumers, and, in general, to public and animal health. Full article

(This article belongs to the Collection Veterinary Microbiology in Farm Animals)

► Show Figures

Figure 1

11 pages, 1434 KiB

Open AccessEditor’s ChoiceArticle

Evaluation of a Novel Infrared Thermography Projection to Assess Udder Health in Primigravid Dairy Heifers

by Patrícia B. A. Simões, Lorenzo Viora, Pieter T. Pepler, Timothy Geraghty, Dominic J. McCafferty and Ruth N. Zadoks

Animals 2022, 12(23), 3410; https://doi.org/10.3390/ani12233410 - 3 Dec 2022

Viewed by 2843

Abstract

Heifer mastitis in early lactation impacts negatively on animal welfare, milk production and longevity. A major challenge for the prevention and control of mastitis in dairy heifers is to establish when intramammary infection occurs because pre-partum secretum sampling is risky. We evaluated a [...] Read more.

Heifer mastitis in early lactation impacts negatively on animal welfare, milk production and longevity. A major challenge for the prevention and control of mastitis in dairy heifers is to establish when intramammary infection occurs because pre-partum secretum sampling is risky. We evaluated a ventrodorsal projection to capture thermal images of the entire udder of primigravid and compared results against caudocranial projection, which is used in lactating cattle. Based on the analysis of 119 heifers and images taken at 2 months and 2 weeks pre-partum, a very strong positive correlation (r = 0.91 and r = 0.96, respectively) was shown between caudocranial and ventrodorsal projections of hind quarters. Quarter maximum gradient temperatures were consistently greater on ventrodorsal projection than on caudocranial projection, and less variable than minimum gradient temperatures. The collection of ventrodorsal images is a simple one-step method involving the imaging of the entire udder in a manner safe for both the cattle and handlers. Together, these results demonstrate that a single projection can be used to scan the entire udder of primigravid dairy heifers in commercial farm conditions, with the potential to implement this as a routine method for the early detection of intramammary infection based on udder surface temperature. Full article

(This article belongs to the Special Issue Diagnostic Imaging Applied to the Internal Medicine of Ruminants)

► Show Figures

Figure 1

13 pages, 2054 KiB

Open AccessEditor’s ChoiceArticle

Effects of Paratuberculosis Vaccination at Different Ages in a Dairy Goat Herd: A 2-Year Follow-Up

by Miguel Fernández, Marcos Royo, Miguel Fuertes, Noive Arteche-Villasol, M. Carmen Ferreras, Julio Benavides and Valentín Pérez

Animals 2022, 12(22), 3135; https://doi.org/10.3390/ani12223135 - 14 Nov 2022

Cited by 8 | Viewed by 2670

Abstract

Vaccination could be considered as an effective method for paratuberculosis control, although controversial, with a need for investigation in some aspects. The objective of this study was to evaluate the effect of vaccination, depending on the age of the animals, on their immune [...] Read more.

Vaccination could be considered as an effective method for paratuberculosis control, although controversial, with a need for investigation in some aspects. The objective of this study was to evaluate the effect of vaccination, depending on the age of the animals, on their immune response, the reduction of paratuberculosis cases, mortality and culled animals in a commercial dairy herd. Goats from three different ages were immunized with the inactivated Gudair^® vaccine. Peripheral antibody and IFN-γ output were evaluated for 21 months post-vaccination (mpv) and intradermal skin tests (IDSTs) for tuberculosis, with avian- and bovine-purified protein derivatives (PPD), were carried out at 6 and at 18 mpv to evaluate the humoral and cellular immune peripheral responses, respectively. The number of dead or culled animals, regardless of the reason, was also monitored and the causes of death determined by pathological examination. A significant increase in the production of IFN-γ was observed in all the vaccinated groups when the blood samples were stimulated with avian PPD, from 3 mpv to 18 mpv, and with bovine PPD, between 3 and 21 mpv. Moreover, serum antibody levels increased between 3 and 21 mpv in all vaccinated groups. The highest levels were found in animals vaccinated at 5 months, and the lowest in adult individuals. No positive reactants to tuberculosis were found by intradermal skin test. No animal losses associated with clinical paratuberculosis were detected in any of the groups. The number of total culled animals was significantly lower in the vaccinated than in the unvaccinated groups, especially on 1.5-month-old vaccinated kids. These results suggest that vaccination of paratuberculosis, especially in young animals, could induce heterologous protection. Full article

(This article belongs to the Section Veterinary Clinical Studies)

► Show Figures

Figure 1

17 pages, 2235 KiB

Open AccessEditor’s ChoiceArticle

Protective Effect of Litchi chinensis Peel Extract-Prepared Nanoparticles on Rabbits Experimentally Infected with Eimeria stiedae

by Dina M. Metwally, Afrah F. Alkhuriji, Ibrahim A. H. Barakat, Hanadi B. Baghdadi, Manal F. El-Khadragy, Wafa Abdullah I. Al-Megrin, Abdullah D. Alanazi and Fatemah E. Alajmi

Animals 2022, 12(22), 3098; https://doi.org/10.3390/ani12223098 - 10 Nov 2022

Cited by 4 | Viewed by 3134

Abstract

The present study used Litchi chinensis peel extract to synthesize silver nanoparticles (AgNPs). This technique is eco-friendly and can be performed in a single step; thus, it has attracted great attention for NPs biosynthesis. Herein, we biosynthesized AgNPs with L. chinensis peel extract [...] Read more.

The present study used Litchi chinensis peel extract to synthesize silver nanoparticles (AgNPs). This technique is eco-friendly and can be performed in a single step; thus, it has attracted great attention for NPs biosynthesis. Herein, we biosynthesized AgNPs with L. chinensis peel extract and examined their anticoccidial activity in rabbit hepatic coccidiosis induced by E. stiedae infection. Thirty-five rabbits were allocated into seven groups: a healthy group (G1), an infected control group (G2), four groups infected before treatment with 10 mg/kg L. chinensis peel extract-biosynthesized AgNPs (G3, G5) or 50 mg/kg amprolium (G4, G6), and rabbits infected after two weeks of pretreatment with 10 mg/kg L. chinensis eel extract-biosynthesized AgNPs (G7). In this study, both pre-and post-treatment with AgNPs produced a substantial reduction in fecal oocyst output, liver enzyme levels, and histopathological hepatic lesions relative to the infected group. In conclusion, L. chinensis peel extract-prepared AgNPs should be considered harmless and efficient in the cure of hepatic coccidiosis in rabbits. Full article

(This article belongs to the Section Veterinary Clinical Studies)

► Show Figures

Figure 1

19 pages, 670 KiB

Open AccessEditor’s ChoiceArticle

Nutritional Composition and Untargeted Metabolomics Reveal the Potential of Tetradesmus obliquus, Chlorella vulgaris and Nannochloropsis oceanica as Valuable Nutrient Sources for Dogs

by Ana R. J. Cabrita, Joana Guilherme-Fernandes, Inês M. Valente, Agostinho Almeida, Sofia A. C. Lima, António J. M. Fonseca and Margarida R. G. Maia

Animals 2022, 12(19), 2643; https://doi.org/10.3390/ani12192643 - 1 Oct 2022

Cited by 30 | Viewed by 4644

Abstract

The growing pet population is questioning the sustainability of the pet food system. Although microalgae may constitute a more sustainable food resource, the assessment of their potential for canine diets is almost non-existent. The present study aimed to evaluate the potential of three [...] Read more.

The growing pet population is questioning the sustainability of the pet food system. Although microalgae may constitute a more sustainable food resource, the assessment of their potential for canine diets is almost non-existent. The present study aimed to evaluate the potential of three microalgae species (Tetradesmus obliquus, Chlorella vulgaris and Nannochloropsis oceanica) grown locally in industrial photobioreactors as alternative food resources for dogs. A detailed characterization of their nutritional composition and metabolomic profile was carried out and related to the nutritional requirements of dogs. Overall, the essential amino acid content exceeded the amounts required for dogs at all life stages, except methionine and cysteine. The three microalgae were deficient in linoleic acid, N. oceanica presented a linolenic acid content below requirements and T. obliquus and C. vulgaris were deficient in arachidonic and eicosapentaenoic acids. The fiber was mainly composed of insoluble dietary fiber. The mineral profile varied greatly with the microalgae species, demonstrating their different potential for dog feeding. Untargeted metabolomics highlighted glycolipids, glycerolipids and phospholipids as the most discriminating compounds between microalgae species. Overall, the results support the potential of T. obliquus, C. vulgaris and N. oceanica as valuable macro- and micro-nutrients sources for dog feeding. Full article

(This article belongs to the Collection The Role of Nutrition in Maintaining Well-Being and Health of Dogs and Cats)

► Show Figures

Figure 1

Journal Menu

Journal Browser

Editor’s Choice Articles

Article

Further Information

Guidelines

MDPI Initiatives

Follow MDPI