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Editor’s Choice Articles

Editor’s Choice articles are based on recommendations by the scientific editors of MDPI journals from around the world. Editors select a small number of articles recently published in the journal that they believe will be particularly interesting to readers, or important in the respective research area. The aim is to provide a snapshot of some of the most exciting work published in the various research areas of the journal.

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18 pages, 4359 KiB  
Article
Deep Learning Methods for Automatic Identification of Male and Female Chickens in a Cage-Free Flock
by Bidur Paneru, Ramesh Bahadur Bist, Xiao Yang, Anjan Dhungana, Samin Dahal and Lilong Chai
Animals 2025, 15(13), 1862; https://doi.org/10.3390/ani15131862 - 24 Jun 2025
Viewed by 553
Abstract
Rooster behavior and activity are critical for egg fertility and hatchability in broiler and layer breeder houses. Desirable roosters are expected to have good leg health, reach sexual maturity, be productive, and show less aggression toward females during mating. However, not all roosters [...] Read more.
Rooster behavior and activity are critical for egg fertility and hatchability in broiler and layer breeder houses. Desirable roosters are expected to have good leg health, reach sexual maturity, be productive, and show less aggression toward females during mating. However, not all roosters are desirable, and low-productive roosters should be removed and replaced. The objectives of this study were to apply an object detection model based on deep learning to identify hens and roosters based on phenotypic characteristics, such as comb size and body size, in a cage-free (CF) environment, and to compare the performance metrics among the applied models. Six roosters were mixed with 200 Lohmann LSL Lite hens during the pre-peak phase in a CF research facility and were marked with different identifications. Deep learning methods, such as You Only Look Once (YOLO) models, were innovated and trained (based on a comb size of up to 2500 images) for the identification of male and female chickens based on comb size and body features. The performance matrices of the YOLOv5u and YOLOv11 models, including precision, recall, mean average precision (mAP), and F1 score, were statistically compared for hen and rooster detection using a one-way ANOVA test at a significance level of p < 0.05. For rooster detection based on comb size, YOLOv5lu, and YOLOv11x variants performed the best among the five variants of each model, with YOLOv5lu achieving a precision of 87.7%, recall of 56.3%, and mAP@0.50 of 60.1%, while YOLOv11x achieved a precision of 86.7%, recall of 65.3%, and mAP@0.50 of 61%. For rooster detection based on body size, YOLOv5xu, and YOLOv11m outperformed other variants, with YOLOv5xu achieving a precision of 88.9%, recall of 77.7%, and mAP@0.50 of 82.3%, while YOLOv11m achieved a precision of 89.0%, recall of 78.8%, and mAP@0.50 of 82.6%. This study provides a reference for automatic rooster monitoring based on comb and body size and offers further opportunities for tracking the activities of roosters in a poultry breeder farm for performance evaluation and genetic selection in the future. Full article
(This article belongs to the Section Animal System and Management)
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14 pages, 1426 KiB  
Article
Rumination Time, Reticulorumen Temperature, and Activity in Relation to Postpartum Health Status in Dairy Cows During Heat Stress
by Szilvia Szalai, Ákos Bodnár, Hedvig Fébel, Mikolt Bakony and Viktor Jurkovich
Animals 2025, 15(11), 1616; https://doi.org/10.3390/ani15111616 - 30 May 2025
Viewed by 537
Abstract
Effective health management during the transition period depends on early disease detection, which can be achieved through continuous monitoring using precision livestock farming tools. This study assessed reticulorumen temperature, rumination time, and activity in dairy cows during the periparturient period under summer heat [...] Read more.
Effective health management during the transition period depends on early disease detection, which can be achieved through continuous monitoring using precision livestock farming tools. This study assessed reticulorumen temperature, rumination time, and activity in dairy cows during the periparturient period under summer heat stress. We hypothesized differences in these parameters between healthy (HE) cows and those developing postpartum disorders (DI). Forty clinically healthy, multiparous cows were monitored from 5 days prepartum to 14 days after calving (days in milk; DIM). A cow was considered healthy and allocated to the HE group (n = 26) if she was not affected by any postpartum health disorders until the end of the study period. A cow was considered diseased and allocated to the DI group (n = 14) if she had been diagnosed with mastitis, metritis, lameness, or ketosis. Weather loggers recorded barn microclimate data, while rumination, activity, and rumen temperature were tracked using a microphone-based sensor in the neck collar (Ruminact HR) and rumen bolus (Smaxtec). THI values remained above 68 throughout the study, peaking at 80, indicating sustained heat stress. Rumen temperature ranged between 39 and 41 °C and moderately correlated with THI (correlation coefficient was 0.27; 95% CI: 0.20; 0.33; p < 0.0001). Both groups exhibited a nadir in rumen temperature at calving, with no differences. Rumination time declined prepartum, reaching its lowest at 2 DIM in DI cows. It was significantly affected by days around calving, postpartum disorders, and THI. Activity increased prepartum and normalized by 4 DIM in HE cows, while DI cows showed higher activity at 4 DIM, stabilizing by 5–7 DIM. These findings underscore the value of precision monitoring tools for early disease detection and intervention. Full article
(This article belongs to the Special Issue Heat Stress and Livestock: Effects on the Physiology)
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11 pages, 1699 KiB  
Communication
The Effect of Ambient Temperature, Relative Humidity, and Temperature–Humidity Index on Stress Hormone and Inflammatory Response in Exercising Adult Standardbred Horses
by Francesca Arfuso, Maria Rizzo, Laura Perillo, Federica Arrigo, Elisabetta Giudice, Giuseppe Piccione, Caterina Faggio and Vincenzo Monteverde
Animals 2025, 15(10), 1436; https://doi.org/10.3390/ani15101436 - 15 May 2025
Viewed by 637
Abstract
In the present study, the changes in the markers of stress and inflammatory responses of 12 Standardbred horses were investigated before and after exercise during late spring and summer. The environmental temperature, relative humidity, and temperature–humidity index (THI) were assessed during this study. [...] Read more.
In the present study, the changes in the markers of stress and inflammatory responses of 12 Standardbred horses were investigated before and after exercise during late spring and summer. The environmental temperature, relative humidity, and temperature–humidity index (THI) were assessed during this study. From horses, blood sampling, measurement of heart and respiratory rate (HR and RR), and measurement of rectal temperature (RT) were performed before exercise (Pre) and within 5 min following the end of the exercise (Post). THI values showed mild stress in June and high stress in July. Direct erythrocyte indices statistically increased after exercise compared to the rest condition (p < 0.05). Higher serum cortisol, α1-, α2-, and β-globulin values were found at Post than at Pre (p < 0.05), whereas lower serum albumin and A/G ratio values were found at Post than at Pre. The HR, RR, and RT statistically increased at Post than at Pre (p < 0.05). The month influenced the values of RR, platelets, cortisol, albumin, α1-, α2-, and β-globulins (p < 0.05). Overall, the results of this study confirmed the well-recognized physiological response of athletic horses to re-establish homeostasis following exercise. Full article
(This article belongs to the Special Issue Diagnosis, Monitoring and Treatment of Physiological Inflammatory in Equids)
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18 pages, 3395 KiB  
Article
Polyphenol-Containing Feed Additive Polygain™ Reduces Methane Production and Intensity from Grazing Dairy Cows Measured Using an Inverse-Dispersion Technique
by Mei Bai, Pragna Prathap, Muhammed Elayadeth-Meethal, Matthew Flavel, Richard Eckard, Frank R. Dunshea, Richard Osei-Amponsah, Mohammad Javed Ashar, Deli Chen and Surinder Chauhan
Animals 2025, 15(7), 926; https://doi.org/10.3390/ani15070926 - 24 Mar 2025
Cited by 2 | Viewed by 1213
Abstract
This study, conducted on a commercial dairy farm using a robotic milking system in Victoria, Australia, examined the effects of Polygain™ (The Product Makers Australia), a polyphenol-rich sugarcane feed material (PRSFM), on CH4 emissions in grazing dairy cattle using an inverse-dispersion model [...] Read more.
This study, conducted on a commercial dairy farm using a robotic milking system in Victoria, Australia, examined the effects of Polygain™ (The Product Makers Australia), a polyphenol-rich sugarcane feed material (PRSFM), on CH4 emissions in grazing dairy cattle using an inverse-dispersion model (IDM) combined with open-path laser techniques. Thirty lactating Holstein Friesian cows (aged 2–5 years with an average body weight of 663 kg and average daily milk production of 28.9 kg) were divided into two dietary treatment groups of fifteen cows each. Before the measurement, the PRSFM (0.25%) was supplemented for 3 weeks as an adaptation period and mixed with pellet feed for the treatment group. Over the 2-week measurement period, CH4 production (MP) was 495 ± 12 and 377 ± 12 (mean ± standard error) g CH4/animal/day for the control and treatment groups, respectively. Methane intensity (MI) was 17.04 and 13.01 g CH4/animal/kg milk/day in the control and treatment groups, respectively. On average, Polygain supplementation reduced MP and MI by 24%. This potential CH4 reduction extrapolated across Australia contributes to a 2.63% reduction in national agricultural emissions. This study underscores the potential of Polygain for CH4 mitigation in dairy cattle. Full article
(This article belongs to the Section Animal Nutrition)
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13 pages, 1449 KiB  
Article
Storage Conditions of Sperm Samples and Gametic Characterization by Sperm Head Morphometry in Drones (Apis mellifera)
by Milagros Cristina Esteso, Adolfo Toledano-Díaz, Cristina Castaño, Mariano Higes, Raquel Martín-Hernández, Agustin López-Goya, Pilar De la Rúa, Belén Martínez-Madrid and Julián Santiago-Moreno
Animals 2025, 15(5), 672; https://doi.org/10.3390/ani15050672 - 26 Feb 2025
Viewed by 861
Abstract
The present study aimed to evaluate an optimal method to transport and store drone sperm samples, as well as to characterize drone spermatozoa through sperm head morphometry. A total of 291 mature drones were used. We performed three experiments. In a first experiment, [...] Read more.
The present study aimed to evaluate an optimal method to transport and store drone sperm samples, as well as to characterize drone spermatozoa through sperm head morphometry. A total of 291 mature drones were used. We performed three experiments. In a first experiment, sperm variables were assessed under different incubation conditions (5 °C, 15 °C, and 37 °C with 5% CO2). Results showed that sperm viability was optimally maintained at 15 °C (p ˂ 0.05). In the second experiment, the supplementation of extender with catalase (200 UI) improved (p ˂ 0.05) the sperm viability and motility during liquid storage at different incubation times. Finally, a morphometric analysis of sperm head was made: length 5.13 µm, width 0.85 µm, area 3.78 µm2, perimeter 15.01 µm, acrosome length 3.50 µm. The variability in sperm head morphometry was calculated by coefficients of variation (CV) within- and between-drones. The CV within-drone was higher than the CV between-drones for all morphometric parameters regardless of hive origin, indicating a high degree of sperm pleomorphism. Full article
(This article belongs to the Special Issue Apiculture and Challenges for Future—2nd Edition)
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12 pages, 779 KiB  
Article
Effect of Stocking Density on Behavioural and Physiological Traits of Laying Hens
by Kamila Janicka, Kamil Drabik, Karolina Wengerska and Iwona Rozempolska-Rucińska
Animals 2025, 15(4), 604; https://doi.org/10.3390/ani15040604 - 19 Feb 2025
Viewed by 697
Abstract
This study analysed stocking density as a major stress factor for laying hens and assessed its impact on selected behavioural and physiological traits of the birds. The study population included 142 birds of the Green-legged Partridge breed (12 males and 132 females). The [...] Read more.
This study analysed stocking density as a major stress factor for laying hens and assessed its impact on selected behavioural and physiological traits of the birds. The study population included 142 birds of the Green-legged Partridge breed (12 males and 132 females). The birds were randomly assigned to three experimental groups of standard, low, and high stocking density, and a modified open-field test was conducted to assess their behavioural traits. The following blood parameters were analysed: corticosterone, cortisol, and testosterone. The behavioural test results demonstrated an impact of stocking density on laying hens’ behaviour. The birds kept at a low density exhibited the greatest diversity in behaviour as well as the most frequent and longest locomotion. There were no differences between the groups in terms of stress hormone results. All groups showed a significant decrease in testosterone levels compared to the control. This may suggest that, despite differences in stocking, the structure was established quickly, resulting in decreased competition and, consequently, a positive impact on the birds. When kept in lower-stocking-density groups with a stable structure, birds are less competitive; this stable structure improves their well-being to a greater extent than the stocking density. Full article
(This article belongs to the Special Issue Advances in Poultry Behaviour and Welfare)
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38 pages, 8260 KiB  
Article
Understanding the Influence of Early-Life Stressors on Social Interaction, Telomere Length, and Hair Cortisol Concentration in Homeless Kittens
by Jennifer Vernick, Chelsea Martin, William Montelpare, Arthur E. Dunham and Karen L. Overall
Animals 2025, 15(3), 446; https://doi.org/10.3390/ani15030446 - 6 Feb 2025
Viewed by 1575
Abstract
The early postnatal period is a critical neurodevelopmental stage characterized by rapid neural maturation and is adversely affected by early-life stressors. This study explored the behavioural, physiological, and epigenetic consequences of early-life stress in a population of homeless rescue kittens. This longitudinal study [...] Read more.
The early postnatal period is a critical neurodevelopmental stage characterized by rapid neural maturation and is adversely affected by early-life stressors. This study explored the behavioural, physiological, and epigenetic consequences of early-life stress in a population of homeless rescue kittens. This longitudinal study included 50 kittens rescued and placed into foster care by the Prince Edward Island Humane Society. They underwent behavioural testing at 8, 10, and 12 weeks of age. Hair cortisol concentration was measured at 8 weeks and served as a physiological marker of the previous 3 months’ cumulative stress response, which, for these kittens, included the late gestation period. A blood sample for relative telomere length measurement was taken at 10–12 weeks to estimate epigenetic changes as young kittens. Data were analyzed with respect to age and performance in all repeated measures tests, status as a stray or a surrender, and the presence of the dam in their foster homes. As expected, the performance of kittens in all tests changed over the 5 weeks of testing. Kittens separated from their mothers exhibited significantly higher hair cortisol concentrations (p = 0.02) and elongated relative telomere lengths (p = 0.04). No correlation was found between hair cortisol concentration and relative telomere lengths (p = 0.99). These results support the need for further study on the effects of epigenetics and early-life stress, both in kittens and across species. Full article
(This article belongs to the Section Animal Physiology)
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11 pages, 1327 KiB  
Article
Effects of a Cannabinoid-Based Phytocomplex (Pain ReliefTM) on Chronic Pain in Osteoarthritic Dogs
by Ruggero Amato, Eleonora Pacifico, Daria Lotito, Valeria Iervolino, Ludovica Pierantoni, Laura Cortese, Nadia Musco, Pietro Lombardi, Vincenzo Mastellone and Maria Elena Pero
Animals 2025, 15(1), 101; https://doi.org/10.3390/ani15010101 - 5 Jan 2025
Cited by 1 | Viewed by 4718
Abstract
Twenty-one adult crossbreed dogs with chronic pain due to severe osteoarthrosis were enrolled in the study (placebo vs. treatment groups). The dogs in the experimental group received the dietary supplement (Pain ReliefTM, Giantec, Isernia, Italy) for 30 days to evaluate its [...] Read more.
Twenty-one adult crossbreed dogs with chronic pain due to severe osteoarthrosis were enrolled in the study (placebo vs. treatment groups). The dogs in the experimental group received the dietary supplement (Pain ReliefTM, Giantec, Isernia, Italy) for 30 days to evaluate its effects on metabolism and pain relief. During the trial, the Helsinki Chronic Pain Index significantly decreased (p < 0.01) in the experimental group, indicating reduced pain and improved quality of life. Additionally, the treated group showed improvements in oxidative stress, demonstrated by a reduction in reactive oxygen metabolites, and an increase in biological antioxidant potential. Interleukins 6 levels decreased in the treated group, while interleukins 10 levels increased, thus suggesting an anti-inflammatory effect of the supplement. Importantly, no adverse effects were observed. Results suggest that Pain ReliefTM is effective in ameliorating osteoarthritis in dogs, improving their quality of life. Full article
(This article belongs to the Collection Nutraceuticals and Animal Physiology: Performance and Welfare)
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14 pages, 1243 KiB  
Review
Social Perception of Zoos and Aquariums: What We Know and How We Know It
by Ana Villarroya, Rafael Miranda, Andrea Pino-del-Carpio and María Casas
Animals 2024, 14(24), 3671; https://doi.org/10.3390/ani14243671 - 19 Dec 2024
Cited by 1 | Viewed by 3040
Abstract
Zoos and aquariums attract millions of visitors around the world. At the same time, the functions they fulfill and the way they do that have been subject to scrutiny from different fields of knowledge. The way societies perceive animals has evolved in recent [...] Read more.
Zoos and aquariums attract millions of visitors around the world. At the same time, the functions they fulfill and the way they do that have been subject to scrutiny from different fields of knowledge. The way societies perceive animals has evolved in recent decades and so has the social perception of institutions that work with them. This scoping review aims to gather studies from the last three decades that shade light on how current societies perceive zoos and aquariums. We selected papers describing quantitative studies published in English in peer-reviewed journals. We reviewed 71 works from different fields of knowledge. The proportion of female authors was noticeably higher than in general science. Most studies were carried out in culturally Western countries, a fact that constitutes a gap in knowledge from a global perspective. We also noticed an underrepresentation of aquariums and of children in the analyzed papers, and a need for more pre–post studies to ascertain the effect of a zoo or aquarium on its visitors. These conclusions point out interesting directions for future studies to help scientists, managers, and citizens design the future of these institutions. Full article
(This article belongs to the Special Issue Zoo Animals: How Actual Zoological Institutions Assess, Ensure, and Promote Their Animals’ Welfare?)
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15 pages, 1767 KiB  
Article
Using Social Network Analysis to Assess ‘Groupness’ in a Mixed-Species Zoo Exhibit of Tufted Capuchins (Sapajus apella) and Squirrel Monkeys (Saimiri sciureus)
by Sophia Daoudi-Simison, Phyllis Lee and Hannah M. Buchanan-Smith
Animals 2024, 14(23), 3360; https://doi.org/10.3390/ani14233360 - 22 Nov 2024
Viewed by 1718
Abstract
Mixed-species groups have been recorded in various primates, including tufted capuchin and squirrel monkeys. Measures of their ‘groupness’ are typically based on factors such as group stability, social interactions, proximity, or behavioural coordination. Social network analysis has become a useful tool for quantifying [...] Read more.
Mixed-species groups have been recorded in various primates, including tufted capuchin and squirrel monkeys. Measures of their ‘groupness’ are typically based on factors such as group stability, social interactions, proximity, or behavioural coordination. Social network analysis has become a useful tool for quantifying relationships among group-living individuals. Here, we apply social network analysis to two captive mixed-species groups of tufted capuchins and squirrel monkeys housed at the Living Links to Human Evolution Research Centre, Royal Zoological Society of Scotland, Edinburgh Zoo, UK. We conducted 183 h of focal observations (three hours per individual, excluding co-observations) and calculated association rates using a simple index ratio. Permutation t-tests were used to assess differences in the overall mixed-species network and network metrics according to species. While the two species exhibited some level of association, they formed separate clusters in the mixed-species networks; however, the East group had more balanced group sizes and showed some signs of closer inter-specific social ties compared to the West group. Our data indicate that, in captivity at least, while these groups co-exist in a small, shared space, they do not form cohesive mixed-species groups. We suggest caution in the assumption of mixed-species groups based on shared space only. Full article
(This article belongs to the Section Zoo Animals)
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22 pages, 6222 KiB  
Article
Mitigating the Effects of Maternal Loss on Harbour Seal Pups in Captive Care
by Susan C. Wilson and Rhiannon Alger
Animals 2024, 14(22), 3264; https://doi.org/10.3390/ani14223264 - 13 Nov 2024
Viewed by 1184
Abstract
Stranded newborn “orphan” harbour seal pups entering captive care are often maintained for some weeks in isolation, mainly as a precaution against the spread of infection. However, this practice raises concerns for the welfare and normal socialisation of pups, who normally spend their [...] Read more.
Stranded newborn “orphan” harbour seal pups entering captive care are often maintained for some weeks in isolation, mainly as a precaution against the spread of infection. However, this practice raises concerns for the welfare and normal socialisation of pups, who normally spend their first post-natal weeks close to their mothers and other seals. The present study recorded and described the behaviour of six paired orphan pups in rehabilitation up to about five weeks of age, provided with free access to water and haul-out areas. The occurrences of resting, following, nosing and body contacts, and aquatic play were recorded and compared qualitatively and quantitatively with the same behaviours of free-living pups with their mothers. The pups entered the water every day, although more often from about 2.5 weeks of age. They displayed to each other the same behaviours that free-living pups display to their mothers, although they engaged in relatively more physical contact, body nosing, and aquatic play. The study has shown that orphan pups maintained in pairs with free water access can act reciprocally as mother substitutes, thereby promoting species-typical primary socialisation and welfare during their early days of captive care. Full article
(This article belongs to the Section Zoo Animals)
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22 pages, 7393 KiB  
Article
Making Noah’s Ark Work for Fishing Cat Conservation: A Blueprint for Connecting Populations across an Interactive Wild Ex Situ Spectrum
by Tiasa Adhya, Simran Singh, Himaja Varma Gottumukkala, Aditya Banerjee, Ishita Chongder, Sulata Maity and P. Anuradha Reddy
Animals 2024, 14(19), 2770; https://doi.org/10.3390/ani14192770 - 25 Sep 2024
Viewed by 2944
Abstract
The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, [...] Read more.
The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, and emotional needs of captive individuals, thereby improving conservation outcomes. Here, we present an integrated framework for the conservation of a globally threatened wetland wild cat species, the fishing cat, by synthesizing optimal ex situ management practices and in situ conservation strategies. Further, we examined the genetic constitution of the founder population in a fishing cat captive breeding program that was recently initiated by the West Bengal Zoo Authority, India and conducted a population viability analysis to suggest how best to maintain the genetic diversity of the population. We found that the present genetic diversity of 56% and maximum carrying capacity of the captive population (30 individuals) can be maintained for more than 100 years with a combination of supplementation and harvesting. Keeping stochastic events in mind, the introduction of two adult males and females to the existing population each year will seamlessly allow the harvesting of two adult males and two adult females every alternate year to supplement wild populations. Further, we adopted the proposed integrated framework to delineate recommendations for the supplementation of wild populations in West Bengal. We used environmental criteria known to influence fishing cat occurrence to identify 21 potential reintroduction zones in the Sundarbans landscape and Terai region in northern West Bengal with habitable areas for the fishing cat that are larger than the maximum known species’ home range. Our study is timely and insightful because it provides a holistic blueprint for implementing the One Plan Approach in safeguarding a threatened species. Full article
(This article belongs to the Special Issue Zoo Animals: How Actual Zoological Institutions Assess, Ensure, and Promote Their Animals’ Welfare?)
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30 pages, 444 KiB  
Review
Nutritional Strategies to Alleviate Stress and Improve Welfare in Dairy Ruminants
by Basiliki Kotsampasi, Maria Anastasia Karatzia, Dimitrios Tsiokos and Stella Chadio
Animals 2024, 14(17), 2573; https://doi.org/10.3390/ani14172573 - 4 Sep 2024
Cited by 7 | Viewed by 3827
Abstract
Dairy ruminants provide a major part of the livestock and agriculture sectors. Due to the increase in world population and the subsequent increase in dairy product demands, the dairy sector has been intensified. Dairy farming intensification and the subsequent increase in animal nutritional [...] Read more.
Dairy ruminants provide a major part of the livestock and agriculture sectors. Due to the increase in world population and the subsequent increase in dairy product demands, the dairy sector has been intensified. Dairy farming intensification and the subsequent increase in animal nutritional demands and the increase in the average global temperature as well have subjected animals to various stress conditions that impact their health and welfare. Various management practices and nutritional strategies have been proposed and studied to alleviate these impacts, especially under heat stress, as well as during critical periods, like the transition period. Some of the nutritional interventions to cope with stress factors and ensure optimal health and production are the inclusion of functional fatty acids and amino acids and feed additives (minerals, prebiotics, probiotics, essential oils and herbs, phytobiotics, enzymes, etc.) that have been proven to regulate animals’ metabolism and improve their antioxidant status and immune function. Thus, these nutritional strategies could be the key to ensuring optimum growth, milk production, and reproduction efficiency. This review summarizes and highlights key nutritional approaches to support the remarkable metabolic adaptations ruminants are facing during the transition period and to reduce heat stress effects and evaluate their beneficial effects on animal physiology, performance, health, as well as welfare. Full article
(This article belongs to the Special Issue Management of Stress Impact on Domestic Animals Physiology and Welfare)
9 pages, 1715 KiB  
Article
The Determination of the Prohibited Herbicide 4,6-Dinitro-Ortho-Cresol (DNOC) in Poisoned Domestic and Wild Animals in Italy
by Rachele Rocchi, Antonella Tinelli, Giuseppe Gatti, Pietro Badagliacca, Antonio Cocco, Maria Chiara Cantelmi, Antonella Damiano, Giampiero Scortichini, Carmine Merola and Antonio Petrini
Animals 2024, 14(17), 2483; https://doi.org/10.3390/ani14172483 - 26 Aug 2024
Viewed by 1733
Abstract
This study reports the determination of DNOC in the baits and gastric contents of poisoned dogs and wild canids collected in the Abruzzo region between 2014 and 2022. A total of 663 samples of the baits and carcasses of domestic and wild animals [...] Read more.
This study reports the determination of DNOC in the baits and gastric contents of poisoned dogs and wild canids collected in the Abruzzo region between 2014 and 2022. A total of 663 samples of the baits and carcasses of domestic and wild animals were analyzed for the determination of DNOC through GC-MS. DNOC was found to be present in 58 cases from 663 analyzed samples, with 49 poison baits and nine gastric content samples from dogs and red foxes. This study confirms for the first time that the banned pesticide DNOC still poisons both domestic and wild animals in the Abruzzo region. It should be considered that this study was carried out on a population of animals from a regional geographical area, and more detailed country-wide studies need to be carried out to obtain the incidence of poisoning from this herbicide in Italy. Furthermore, these findings emphasize the importance of considering this chemical in differential diagnosis during toxicological investigations of animal poisoning. Full article
(This article belongs to the Special Issue Environmental Toxicology: The Effects of Chemical Exposure on Animal Physiological and Biochemical Indicators)
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14 pages, 11156 KiB  
Article
Chromium Affects Mitochondrial Function, Leading to Apoptosis and Autophagy in Turtle Primary Hepatocytes
by Shuqin Lin, Yunjuan Xiao, Jing Lin, Yue Yuan, Haitao Shi, Meiling Hong and Li Ding
Animals 2024, 14(16), 2403; https://doi.org/10.3390/ani14162403 - 19 Aug 2024
Cited by 1 | Viewed by 1935
Abstract
Hexavalent chromium (Cr(VI)), a pervasive industrial contaminant, is highly toxic to both humans and animals. However, its effects on turtles are largely unexplored. Our study aimed to investigate the toxic effects of Cr(VI) on the Reeves’ turtles (Mauremys reevesii) primary hepatocytes. [...] Read more.
Hexavalent chromium (Cr(VI)), a pervasive industrial contaminant, is highly toxic to both humans and animals. However, its effects on turtles are largely unexplored. Our study aimed to investigate the toxic effects of Cr(VI) on the Reeves’ turtles (Mauremys reevesii) primary hepatocytes. We exposed hepatocytes to two concentrations (25 μM and 50 μM) of Cr(VI) for 24 h. The results showed that compared to controls, Cr(VI)-treated cells showed elevated antioxidant enzyme activity (catalase (CAT) and superoxide dismutase (SOD)) and increased reactive oxygen species (ROS) levels. Adenosine triphosphatae (ATP) levels decreased, indicating mitochondrial dysfunction. Additionally, we found significant changes in mitochondrial dynamics related genes, with downregulation of mitofusin 2 (Mfn2) and silent information regulator 1 (SIRT1) and a decrease in sirtuin 3 (SIRT3) and tumor protein 53 (p53) mRNA levels. Annexin V-FITC fluorescence staining-positive cells increased with higher Cr(VI) concentrations, marked by elevated bcl-2-associated X protein (Bax) and cysteinyl aspartate specific proteinase (Caspase3) mRNA levels and reduced B-cell lymphoma-2 (Bcl2) expression. Autophagy-related genes were also affected, with increased microtubule-associated protein 1 light chain 3 (LC3-I), microtubule-associated protein light chain 3II (LC3-II), unc-51-like autophagy-activating kinase 1 (ULK1), and sequestosome 1 (p62/SQSTM1) mRNA levels and decreased mammalian target of rapamycin (mTOR) and Beclin1 expression. Taken together, Cr(VI) promotes cell apoptosis and autophagy in turtle hepatocytes by inducing oxidative stress and disrupting mitochondrial function. These findings highlight the serious health risks posed by Cr(VI) pollution and emphasize the need for protecting wild turtle populations. Full article
(This article belongs to the Special Issue Aquatic Animal Medicine and Pathology)
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13 pages, 637 KiB  
Review
Environmental Enrichment in Rabbit Husbandry: Comparative Impacts on Performance and Welfare
by Karim El-Sabrout, Anjum Sherasiya, Sohail Ahmad, Sarah Aggag, Eleonora Nannoni, Damiano Cavallini and Giovanni Buonaiuto
Animals 2024, 14(16), 2367; https://doi.org/10.3390/ani14162367 - 15 Aug 2024
Cited by 17 | Viewed by 4526
Abstract
Rabbits are highly susceptible to environmental stress. Such stress, stemming from conventional housing conditions, can negatively impact well-being and productivity. Some of these negative consequences are increased susceptibility to diseases and infections and reduced growth rates and reproductive performance, as well as increased [...] Read more.
Rabbits are highly susceptible to environmental stress. Such stress, stemming from conventional housing conditions, can negatively impact well-being and productivity. Some of these negative consequences are increased susceptibility to diseases and infections and reduced growth rates and reproductive performance, as well as increased behavioral issues such as aggression. Environmental enrichment is the modification of the environment in which a domesticated animal lives in order to meet the species’ behavioral needs. The objective of providing enrichment is to facilitate animals in expressing natural behaviors, thereby preventing behavioral frustration and negative affective states. Several inexpensive and safe materials can be used to enrich rabbit enclosures. This review emphasizes the significance of implementing diverse environmental enrichments to alleviate stress in rabbit farming. It summarizes their effects on animal performance and welfare while exploring potential future perspectives in this field. Full article
(This article belongs to the Special Issue Management of Stress Impact on Domestic Animals Physiology and Welfare)
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20 pages, 18545 KiB  
Article
Stress Responses in Horses Housed in Different Stable Designs during Summer in a Tropical Savanna Climate
by Chanoknun Poochipakorn, Thita Wonghanchao, Kanokpan Sanigavatee and Metha Chanda
Animals 2024, 14(15), 2263; https://doi.org/10.3390/ani14152263 - 4 Aug 2024
Cited by 1 | Viewed by 1961
Abstract
Single-confinement housing can pose welfare risks to domestic horses. This study investigated horses’ stress responses when confined to single stalls in different stable designs in a tropical savanna region to address a gap in the literature. In total, 23 horses were assigned to [...] Read more.
Single-confinement housing can pose welfare risks to domestic horses. This study investigated horses’ stress responses when confined to single stalls in different stable designs in a tropical savanna region to address a gap in the literature. In total, 23 horses were assigned to a stable with a central corridor and solid external walls (A) (N = 8), a stable with one side corridor and solid external walls (B) (N = 6), or a stable with a central corridor and no solid external walls (C) (N = 9). Air velocity, relative humidity, air temperature, and noxious gases were measured inside the stables, and the heart rate and HRV of the horses were also determined. The relative humidity was lower in stable C than in stable A (p < 0.05), while the air temperature was higher in stable C than in stable B (p < 0.05) during the day. The airflow and ammonia levels were higher in stable C than in stables B and A (p < 0.01–0.0001). Overall, horses’ HRV in stable A was lower than in those in stables B and C (p < 0.05–0.01). Horses in stable A tended to experience more stress than those in other stables. Full article
(This article belongs to the Special Issue Exploring Stress Responses in Farm Animals: A Focus on Physiological and Behavioral Aspects)
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11 pages, 1990 KiB  
Article
The Interplay of TLR-NFκB Signalling Pathway and Functional Immune-Related Enzymes in the Inflammatory Response of Ciona robusta
by Luca Bisanti, Claudia La Corte, Mariano Dara, Federica Bertini, Jacopo Vizioli, Maria Giovanna Parisi, Matteo Cammarata and Daniela Parrinello
Animals 2024, 14(15), 2169; https://doi.org/10.3390/ani14152169 - 25 Jul 2024
Cited by 3 | Viewed by 1371
Abstract
The close phylogenetic relationship between ascidians (Tunicata) and vertebrates makes them a powerful model for studying the innate immune system. To better understand the nature and dynamics of immune responses and the mechanisms through which bacterial infections are detected and translated into inflammation [...] Read more.
The close phylogenetic relationship between ascidians (Tunicata) and vertebrates makes them a powerful model for studying the innate immune system. To better understand the nature and dynamics of immune responses and the mechanisms through which bacterial infections are detected and translated into inflammation in Ciona robusta, we applied an approach combining in vivo lipopolysaccharide (LPS) stimulation, immune-labelling techniques and functional enzymatic analyses. The immunohistochemistry showed that Toll-like receptor 4 (TLR4) and nuclear factor kappa B (NFκB) were expressed during the inflammatory pharynx response 4 h post-LPS, with the formation of nodules in pharynx vessel lumen. Also, the endothelium vessels were involved in the inflammatory response. Observations of histological sections from naive and buffer-inoculated ascidians confirmed an immuno-positive response. Enzyme immune parameters—which included the activity of phenoloxidase, glutathione peroxidase, lysozyme, alkaline phosphatase and esterase—showed up-modulation 4 h after LPS injection, confirming their participation during ascidian inflammatory response. These findings provide new insights into the mechanisms underlying the LPS-induced C. robusta response and suggest that a broad innate immune mechanism, as in vertebrates, is involved in the regulation of inflammatory responses. Further findings in this direction are needed to cover knowledge gaps regarding the organized set of molecular and cellular networks involved in universal immune interactions with pathogens. Full article
(This article belongs to the Section Aquatic Animals)
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17 pages, 1645 KiB  
Article
Different Founding Effects Underlie Dominant Blue Eyes (DBE) in the Domestic Cat
by Marie Abitbol, Caroline Dufaure de Citres, Gabriela Rudd Garces, Gesine Lühken, Leslie A. Lyons and Vincent Gache
Animals 2024, 14(13), 1845; https://doi.org/10.3390/ani14131845 - 21 Jun 2024
Cited by 1 | Viewed by 6367
Abstract
During the last twenty years, minimal white spotting associated with blue eyes was selected by feline breeders to create the Altai, Topaz, and Celestial breeds. Additionally, certain breeders introduced this trait in their lineages of purebred cats. The trait has been called “dominant [...] Read more.
During the last twenty years, minimal white spotting associated with blue eyes was selected by feline breeders to create the Altai, Topaz, and Celestial breeds. Additionally, certain breeders introduced this trait in their lineages of purebred cats. The trait has been called “dominant blue eyes (DBE)” and was confirmed to be autosomal dominant in all lineages. DBE was initially described in outbred cats from Kazakhstan and Russia and in two purebred lineages of British cats from Russia, as well as in Dutch Maine Coon cats, suggesting different founding effects. We have previously identified two variants in the Paired Box 3 (PAX3) gene associated with DBE in Maine Coon and Celestial cats; however, the presence of an underlying variant remains undetermined in other DBE breeding lines. Using a genome-wide association study, we identified a single region on chromosome C1 that was associated with DBE in British cats. Within that region, we identified PAX3 as the strongest candidate gene. Whole-genome sequencing of a DBE cat revealed an RD-114 retrovirus LTR (long terminal repeat) insertion within PAX3 intron 4 (namely NC_018730.3:g.206975776_206975777insN[433]) known to contain regulatory sequences. Using a panel of 117 DBE cats, we showed that this variant was fully associated with DBE in two British lineages, in Altai cats, and in some other DBE lineages. We propose that this NC_018730.3:g.206975776_206975777insN[433] variant represents the DBEALT (Altai Dominant Blue Eye) allele in the domestic cat. Finally, we genotyped DBE cats from 14 lineages for the three PAX3 variants and showed that they were not present in four lineages, confirming genetic heterogeneity of the DBE trait in the domestic cat. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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11 pages, 2290 KiB  
Article
Comparative Cytogenetics of the Malagasy Ground Geckos of the Paroedura bastardi and Paroedura picta Species Groups
by Marcello Mezzasalma, Gaetano Odierna, Rachele Macirella and Elvira Brunelli
Animals 2024, 14(11), 1708; https://doi.org/10.3390/ani14111708 - 6 Jun 2024
Viewed by 1220
Abstract
We present a comparative chromosome study of several taxa of the Malagasy ground geckos of the Paroedura bastardi and P. picta species groups. We employed a preliminary molecular analysis using a trait of the mitochondrial 16S rRNA gene (of about 570 bp) to [...] Read more.
We present a comparative chromosome study of several taxa of the Malagasy ground geckos of the Paroedura bastardi and P. picta species groups. We employed a preliminary molecular analysis using a trait of the mitochondrial 16S rRNA gene (of about 570 bp) to assess the taxonomic status of the samples studied and a cytogenetic analysis with standard karyotyping (5% Giemsa solution), silver staining (Ag–NOR staining) and sequential C-banding (C-banding + Giemsa and + fluorochromes). Our results show that all the taxa studied of the P. bastardi group (P. ibityensis, P. rennerae and P. cf. guibeae) have a similar karyotype composed of 2n = 34 chromosomes, with two metacentric pairs (1 and 3) and all other pairs being acrocentric. Chromosome diversification in the P. bastardi group was mainly linked to the diversification of heteromorphic sex chromosome systems (ZZ/ZW) in P. ibityensis and P. rennerae, while no heteromorphic sex chromosome pair was found in P. cf. guibeae. The two taxa investigated of the P. picta species group (here named P. picta and P. cf. picta based on molecular data) showed the same chromosome number of 2n = 36, mostly acrocentric elements, but differed in the number of metacentric elements, probably as a result of an inversion at chromosome pair 2. We highlight that the genus Paroedura is characterized by the independent diversification of heterogametic sex chromosomes in different evolutionary lineages and, similarly to other phylogenetically related gecko genera, by a progressive formation of a biarmed element by means of tandem fusions and inversions of distinct pairs. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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16 pages, 1004 KiB  
Article
A Study on Mineral Oil Hydrocarbons (MOH) Contamination in Pig Diets and Its Transfer to Back Fat and Loin Tissues
by Paula Albendea, Chiara Conchione, Luca Menegoz Ursol and Sabrina Moret
Animals 2024, 14(10), 1450; https://doi.org/10.3390/ani14101450 - 13 May 2024
Cited by 2 | Viewed by 1909
Abstract
This study assessed saturated mineral oil hydrocarbons (MOSH) and aromatic mineral oil hydrocarbons (MOAH) levels in grower–finisher feeds for pigs supplemented with 5% crude palm oil (CP), crude olive pomace oil (COP), olive pomace acid oil (OPA), or a blend of CP and [...] Read more.
This study assessed saturated mineral oil hydrocarbons (MOSH) and aromatic mineral oil hydrocarbons (MOAH) levels in grower–finisher feeds for pigs supplemented with 5% crude palm oil (CP), crude olive pomace oil (COP), olive pomace acid oil (OPA), or a blend of CP and OPA (50:50, w/w); the contribution of the lipid source to that contamination; and the ability of pigs to accumulate MOH in back fat and loin tissues after 60 days of trial. MOSH and MOAH were analyzed with liquid chromatography (LC)–gas chromatography (GC)–flame ionization detection (FID) after sample preparation. Among the lipid sources, CP had the lowest MOH levels, but CP feeds showed the highest contamination. This, along with the different MOSH profiles, indicated the presence of more significant contamination sources in the feeds than the lipid source. The higher MOH contamination in CP feeds was reflected in the highest MOSH levels in pig back fat, whereas MOAH were not detected in animal tissues. Also, MOSH bioaccumulation in pig tissues was influenced by the carbon chain length. In conclusion, feed manufacturing processes can determine the MOSH contamination present in animal adipose tissues that can be included in human diets. Full article
(This article belongs to the Special Issue The Influence of Environment and Management on the Animal-Origin Products)
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23 pages, 6104 KiB  
Article
Maternal Effects and Trophodynamics Drive Interannual Larval Growth Variability of Atlantic Bluefin Tuna (Thunnus thynnus) from the Gulf of Mexico
by José M. Quintanilla, Ricardo Borrego-Santos, Estrella Malca, Rasmus Swalethorp, Michael R. Landry, Trika Gerard, John Lamkin, Alberto García and Raúl Laiz-Carrión
Animals 2024, 14(9), 1319; https://doi.org/10.3390/ani14091319 - 28 Apr 2024
Viewed by 1566
Abstract
Two cohorts of Atlantic bluefin tuna (Thunnus thynnus) larvae were sampled in 2017 and 2018 during the peak of spawning in the Gulf of Mexico (GOM). We examined environmental variables, daily growth, otolith biometry and stable isotopes and found that the [...] Read more.
Two cohorts of Atlantic bluefin tuna (Thunnus thynnus) larvae were sampled in 2017 and 2018 during the peak of spawning in the Gulf of Mexico (GOM). We examined environmental variables, daily growth, otolith biometry and stable isotopes and found that the GOM18 cohort grew at faster rates, with larger and wider otoliths. Inter and intra-population analyses (deficient vs. optimal growth groups) were carried out for pre- and post-flexion developmental stages to determine maternal and trophodynamic influences on larval growth variability based on larval isotopic signatures, trophic niche sizes and their overlaps. For the pre-flexion stages in both years, the optimal growth groups had significantly lower δ15N, implying a direct relationship between growth potential and maternal inheritance. Optimal growth groups and stages for both years showed lower C:N ratios, reflecting a greater energy investment in growth. The results of this study illustrate the interannual transgenerational trophic plasticity of a spawning stock and its linkages to growth potential of their offsprings in the GOM. Full article
(This article belongs to the Special Issue Fisheries Larval Ecology and Oceanography)
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16 pages, 866 KiB  
Article
Effects of Garlic Oil and Cinnamaldehyde on Sheep Rumen Fermentation and Microbial Populations in Rusitec Fermenters in Two Different Sampling Periods
by Jairo García-Rodríguez, Cristina Saro, Iván Mateos, María Dolores Carro and María José Ranilla
Animals 2024, 14(7), 1067; https://doi.org/10.3390/ani14071067 - 30 Mar 2024
Viewed by 2091
Abstract
Garlic oil (GO) and cinnamaldehyde (CIN) have shown potential to modify rumen fermentation. The aim of this study was to assess the effects of GO and CIN on rumen fermentation, microbial protein synthesis (MPS), and microbial populations in Rusitec fermenters fed a mixed [...] Read more.
Garlic oil (GO) and cinnamaldehyde (CIN) have shown potential to modify rumen fermentation. The aim of this study was to assess the effects of GO and CIN on rumen fermentation, microbial protein synthesis (MPS), and microbial populations in Rusitec fermenters fed a mixed diet (50:50 forage/concentrate), as well as whether these effects were maintained over time. Six fermenters were used in two 15-day incubation runs. Within each run, two fermenters received no additive, 180 mg/L of GO, or 180 mg/L of CIN. Rumen fermentation parameters were assessed in two periods (P1 and P2), and microbial populations were studied after each of these periods. Garlic oil reduced the acetate/propionate ratio and methane production (p < 0.001) in P1 and P2 and decreased protozoal DNA concentration and the relative abundance of fungi and archaea after P1 (p < 0.05). Cinnamaldehyde increased bacterial diversity (p < 0.01) and modified the structure of bacterial communities after P1, decreased bacterial DNA concentration after P2 (p < 0.05), and increased MPS (p < 0.001). The results of this study indicate that 180 mg/L of GO and CIN promoted a more efficient rumen fermentation and increased the protein supply to the animal, respectively, although an apparent adaptive response of microbial populations to GO was observed. Full article
(This article belongs to the Section Animal Nutrition)
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19 pages, 3141 KiB  
Systematic Review
Can the Inclusion of Forage Chicory in the Diet of Lactating Dairy Cattle Alter Milk Production and Milk Fatty Acid Composition? Findings of a Multilevel Meta-Analysis
by Mancoba C. Mangwe, Racheal H. Bryant, Antonia Olszewski, Hitihamy Mudiyanselage Gayani P. Herath and Omar Al-Marashdeh
Animals 2024, 14(7), 1002; https://doi.org/10.3390/ani14071002 - 25 Mar 2024
Viewed by 1921
Abstract
In traditional ryegrass/white clover (Lolium perenne L./Trifolium repens L.) pastoral systems, forage herbs such as chicory (Cichorium intybus L.) present an opportunity to fill feed deficits during late spring and summer. Although multiple research publications have evaluated the efficacy of [...] Read more.
In traditional ryegrass/white clover (Lolium perenne L./Trifolium repens L.) pastoral systems, forage herbs such as chicory (Cichorium intybus L.) present an opportunity to fill feed deficits during late spring and summer. Although multiple research publications have evaluated the efficacy of chicory for enhancing milk production and milk fatty acid (FA) profile, no publication has quantitatively synthesised the body of research. This systematic review and meta-analysis examined the effect of chicory on milk production and composition, as well as on the milk fatty acid composition of dairy cattle. A total of 29 comparisons from 15 unique research publications involving 597 dairy cattle were used to develop a dataset for analysis. Three-level random-effect and robust variance estimator models were used to account for the hierarchical structure of the data and the dependency of effect sizes within publications. Chicory inclusion increased milk yield when compared to grass-based diets {weighted mean difference (WMD) = 1.07 (95% CI 0.54–1.60) kg/cow/d, p < 0.001}, but it provided a similar milk yield when compared to other forages such as legumes and herbs {dicots; WMD = −0.30, (95% CI −89–0.29) kg/cow/day, p = 0.312}. Increases in milk yield were congruent with differences in DM intake (p = 0.09) and ME intakes (p = 0.003), being similar in chicory-fed and dicot-fed cows but higher than grass-fed cows. Chicory feeding’s effect on milk solids was twice as high during mid lactation {154 days in milk; WMD = 0.13, (95% 0.081–0.175) kg/cow/day, p < 0.001} as during late lactation {219 days in milk; WMD = 0.06, (95% 0.003–0.13) kg/cow/day, p = 0.041}. In line with milk yield, greater and more significant effect sizes were found for alpha linolenic acid {ALA; WMD = 0.20 (95% CI 0.06–0.35) g/100 g FA, p = 0.011} when chicory was compared to grass species only. Comparing chicory with dicots suggests that chicory inclusion did not impact ALA concentrations {WMD = 0.001 (95% CI −0.02–0.2) g/100 g FA, p = 0.99}. There were no differences in conjugated linoleic acid concentration in the milk of cows fed chicory or control diets. The study provides empirical evidence of chicory’s efficacy for improved milk production and milk fatty acid composition. Full article
(This article belongs to the Special Issue Sustainable Management of Ruminants: Feed Utilization, Nitrogen Dynamics, Physiology, and Metabolism)
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10 pages, 289 KiB  
Article
Evaluation of Two Species of Macroalgae from Azores Sea as Potential Reducers of Ruminal Methane Production: In Vitro Ruminal Assay
by Helder P. B. Nunes, Cristiana S. A. M. Maduro Dias, Nuno V. Álvaro and Alfredo E. S. Borba
Animals 2024, 14(6), 967; https://doi.org/10.3390/ani14060967 - 20 Mar 2024
Cited by 8 | Viewed by 2392
Abstract
The utilisation of seaweeds as feed supplements has been investigated for their potential to mitigate enteric methane emissions from ruminants. Enteric methane emissions are the primary source of direct greenhouse gas emissions in livestock and significantly contribute to anthropogenic methane emissions worldwide. The [...] Read more.
The utilisation of seaweeds as feed supplements has been investigated for their potential to mitigate enteric methane emissions from ruminants. Enteric methane emissions are the primary source of direct greenhouse gas emissions in livestock and significantly contribute to anthropogenic methane emissions worldwide. The aim of the present study is to evaluate the nutritional role and the in vitro effect on cumulative gas and methane production of Asparagopsis taxiformis (native species) and Asparagopsis armata (invasive species), two species of red algae from the Azorean Sea, as well as the ability to reduce biogas production when incubated with single pasture (Lolium perenne and Trifollium repens) as substrate. Four levels of concentrations marine algae were used (1.25%, 2.25%, 5%, and 10% DM) and added to the substrate to evaluate ruminal fermentation using the in vitro gas production technique. The total amount of gas and methane produced by the treatment incubation was recorded during 72 h of incubation. The results indicate that both algae species under investigation contain relatively high levels of protein (22.69% and 24.23%, respectively, for Asparagopsis taxiformis and Asparagopsis armata) and significant amounts of minerals, namely magnesium (1.15% DM), sodium (8.6% DM), and iron (2851 ppm). Concerning in vitro ruminal fermentation, it was observed that A. taxiformis can reduce enteric methane production by approximately 86%, during the first 24 h when 5% is added. In the same period and at the same concentration, A. armata reduced methane production by 34%. Thus, it can be concluded that Asparagopsis species from the Azorean Sea have high potential as a protein and mineral supplement, in addition to enabling a reduction in methane production from rumen fermentation. Full article
(This article belongs to the Special Issue Algae in Animal Nutrition)
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10 pages, 947 KiB  
Article
Effects of Sugarcane-Derived Polyphenol Supplementation on Methane Production and Rumen Microbial Diversity of Second-Cross Lambs
by Pragna Prathap, Surinder S. Chauhan, Matthew Flavel, Shane Mitchell, Jeremy J. Cottrell, Brian J. Leury and Frank R. Dunshea
Animals 2024, 14(6), 905; https://doi.org/10.3390/ani14060905 - 14 Mar 2024
Cited by 7 | Viewed by 2595
Abstract
The objective of this study was to evaluate the effects of feeding sugarcane-derived polyphenolic supplement (Polygain, The Product Makers Australia, Keysborough, VIC, Australia) on enteric methane (CH4) emission, rumen microbiota, and performance of second-cross lambs. For this purpose, 24 Poll Dorset [...] Read more.
The objective of this study was to evaluate the effects of feeding sugarcane-derived polyphenolic supplement (Polygain, The Product Makers Australia, Keysborough, VIC, Australia) on enteric methane (CH4) emission, rumen microbiota, and performance of second-cross lambs. For this purpose, 24 Poll Dorset × (Border Leicester × Merino) lambs were allocated to 3 different treatments: Control (C), 0.25% Polygain (0.25 PG), and 1% Polygain (1 PG) diets with a uniform basal feed (25% cracked wheat grain, 25% cracked barley grain, 25% oaten chaff, 25% lucerne chaff). Both doses of Polygain reduced the total CH4 production (g/day; p = 0.006), CH4 yield (CH4, g/kg of dry matter intake; p = 0.003) and CH4 intensity (CH4, g/kg of BW; p = 0.003). Dry matter intake tended to be greater (p = 0.08) in sheep fed 1 PG compared to the C group, with the 0.25 PG group being intermediate. The average daily gain of the lambs was improved (p = 0.03) with 1% Polygain supplementation. The relative abundance of genera Methanobrevibacter_unidentified, Methanomethylophilaceae_uncultured, Methanogenic archaeon mixed culture ISO4-G1, Methanosphaera uncultured rumen methanogen, Methanogenic archaeon ISO4-H5, and Methanobrevibacter boviskoreani JH1 were reduced with Polygain supplementation. In conclusion, feeding Polygain reduced lambs’ enteric CH4 emissions, altered the rumen microbiome, and improved the growth performance of lambs. Full article
(This article belongs to the Section Animal Nutrition)
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14 pages, 2035 KiB  
Article
Influence of Different Plant Extracts on CYP-Mediated Skatole and Indole Degradation in Pigs
by Philipp Marro, Raffael Wesoly and Volker Stefanski
Animals 2024, 14(6), 888; https://doi.org/10.3390/ani14060888 - 13 Mar 2024
Cited by 3 | Viewed by 1854
Abstract
One of the primary substances responsible for the unpleasant odor in boar meat is skatole. Enzymes belonging to the cytochrome P450 (CYP) family play a pivotal role in the hepatic clearance of skatole. This study aimed to investigate the impact of oregano essential [...] Read more.
One of the primary substances responsible for the unpleasant odor in boar meat is skatole. Enzymes belonging to the cytochrome P450 (CYP) family play a pivotal role in the hepatic clearance of skatole. This study aimed to investigate the impact of oregano essential oil (OEO), Schisandra chinensis extract (SC), and garlic essential oil (GEO) on hepatic CYP2E1 and CYP2A activity in pigs. In three consecutive trials, cannulated castrated male pigs were provided with a diet containing 0.2–0.3% of one of these plant extracts. Following a 14-day feeding period, the animals were slaughtered, and liver and fat samples were collected. The findings indicate that the activities of CYP2E1 were unaffected by any treatment. However, GEO treatment demonstrated a significant reduction in CYP2A activity (p < 0.05). Pigs treated with GEO also exhibited a notable increase in skatole concentrations in both plasma and adipose tissue. In contrast, animals fed SC displayed elevated skatole concentrations in plasma but not in fat tissue. OEO did not influence skatole concentrations in either blood or fat. Furthermore, the study revealed that a supplementation of 6 g GEO per animal per day induced a significant increase in skatole concentrations in blood plasma within 24 h. Full article
(This article belongs to the Special Issue Plant Extracts as Feed Additives in Animal Nutrition and Health)
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38 pages, 6808 KiB  
Review
Effects of Nutritional Factors on Fat Content, Fatty Acid Composition, and Sensorial Properties of Meat and Milk from Domesticated Ruminants: An Overview
by Eric N. Ponnampalam, Hasitha Priyashantha, Janak K. Vidanarachchi, Ali Kiani and Benjamin W. B. Holman
Animals 2024, 14(6), 840; https://doi.org/10.3390/ani14060840 - 8 Mar 2024
Cited by 38 | Viewed by 10234
Abstract
The meat and milk products from domesticated ruminants are important foods within a balanced diet, offering a rich source of energy, protein, fats, minerals, and vitamins. The sensorial properties of meat and milk are mainly linked to their fat content and fatty acid [...] Read more.
The meat and milk products from domesticated ruminants are important foods within a balanced diet, offering a rich source of energy, protein, fats, minerals, and vitamins. The sensorial properties of meat and milk are mainly linked to their fat content and fatty acid composition, which are influenced by the feeding background or nutrient composition of diets. While several studies have investigated the nutritional effects on the fat content and fatty acid profile of ruminant meat and milk, as well as their relationship with sensorial properties, a comprehensive overview of these effects is lacking. This paper summarises current literature and discusses changes to fatty acid composition (including ω-3 concentrations), fattiness, and associated quality traits of sheep, goat, beef cattle, alpaca, and llama meat that can be achieved by using different forages or feeds in a total mixed ration. Additionally, it presents the shelf life and nutritional value of meat, milk, and cheeses from the milk of dairy cattle, buffalo, goats, and sheep as influenced by a ruminant diet. Further advancement in these areas will promote the sustainability of ruminant production and its associated feeding systems in achieving premium quality animal-derived foods. Full article
(This article belongs to the Special Issue Alternative Forages, Novel Feeds, and Supplementation Effects on Animal Performance and Product Quality: Meat, Milk, and Natural Fibres)
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16 pages, 3470 KiB  
Article
Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis
by Rafael González-Cano, Ana González-Martínez, Manuel Ramón, Miriam González Serrano, Miguel Moreno Millán, Alejandro Rubio de Juan and Evangelina Rodero Serrano
Animals 2024, 14(5), 793; https://doi.org/10.3390/ani14050793 - 4 Mar 2024
Viewed by 1927
Abstract
Most of the previous studies on the genetic variability in Spanish “Berrenda” breeds have been carried out using DNA microsatellites. The present work aimed to estimate the genetic diversity, population structure, and potential genetic differences among individuals of both Berrenda breeds and groups [...] Read more.
Most of the previous studies on the genetic variability in Spanish “Berrenda” breeds have been carried out using DNA microsatellites. The present work aimed to estimate the genetic diversity, population structure, and potential genetic differences among individuals of both Berrenda breeds and groups based on the presence of the Robertsonian chromosomal translocation, rob (1;29). A total of 373 samples from animals belonging to the two breeds, including 169 cases diagnosed as rob (1;29)-positive, were genotyped using an SNP50K chip. The genetic diversity at the breed level did not show significant differences, but it was significantly lower in those subpopulations containing the rob (1;29). Runs of homozygosity identified a region of homozygosity on chromosome 6, where the KIT (KIT proto-oncogene, receptor tyrosine kinase) gene, which determines the typical spotted coat pattern in both breeds, is located. The four subpopulations considered showed minor genetic differences. The regions of the genome that most determined the differences between the breeds were observed on chromosomes 4, 6, 18, and 22. The presence of this Robertsonian translocation did not result in sub-structuring within each of the breeds considered. To improve the reproductive performance of Berrenda breeds, it would be necessary to implement strategies considering the involvement of potential breeding stock carrying rob (1;29). Full article
(This article belongs to the Section Animal Genetics and Genomics)
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13 pages, 233 KiB  
Article
Estimation of Protein and Amino Acid Requirements in Layer Chicks Depending on Dynamic Model
by Miao Liu, Zhi-Yuan Xia, Hong-Lin Li, Yu-Xuan Huang, Alainaa Refaie, Zhang-Chao Deng and Lv-Hui Sun
Animals 2024, 14(5), 764; https://doi.org/10.3390/ani14050764 - 29 Feb 2024
Cited by 5 | Viewed by 2651
Abstract
Four trials were conducted to establish a protein and amino acid requirement model for layer chicks over 0–6 weeks by using the analytical factorization method. In trial 1, a total of 90 one-day-old Jing Tint 6 chicks with similar body weight were selected [...] Read more.
Four trials were conducted to establish a protein and amino acid requirement model for layer chicks over 0–6 weeks by using the analytical factorization method. In trial 1, a total of 90 one-day-old Jing Tint 6 chicks with similar body weight were selected to determine the growth curve, carcass and feather protein deposition, and amino acid patterns of carcass and feather proteins. In trials 2 and 3, 24 seven-day-old and 24 thirty-five-day-old Jing Tint 6 chicks were selected to determine the protein maintenance requirements, amino acid pattern, and net protein utilization rate. In trial 4, 24 ten-day-old and 24 thirty-eight-day-old Jing Tint 6 chicks were selected to determine the standard terminal ileal digestibility of amino acids. The chicks were fed either a corn–soybean basal diet, a low nitrogen diet, or a nitrogen-free diet throughout the different trials. The Gompertz equation showed that there is a functional relationship between body weight and age, described as BWt(g) = 2669.317 × exp(−4.337 × exp(−0.019t)). Integration of the test results gave a comprehensive dynamic model equation that could accurately calculate the weekly protein and amino acid requirements of the layer chicks. By applying the model, it was found that the protein requirements for Jing Tint 6 chicks during the 6-week period were 21.15, 20.54, 18.26, 18.77, 17.79, and 16.51, respectively. The model-predicted amino acid requirements for Jing Tint 6 chicks during the 6-week period were as follows: Aspartic acid (0.992–1.284), Threonine (0.601–0.750), Serine (0.984–1.542), Glutamic acid (1.661–1.925), Glycine (0.992–1.227), Alanine (0.909–0.961), Valine (0.773–1.121), Cystine (0.843–1.347), Methionine (0.210–0.267), Isoleucine (0.590–0.715), Leucine (0.977–1.208), Tyrosine (0.362–0.504), Phenylalanine (0.584–0.786), Histidine (0.169–0.250), Lysine (0.3999–0.500), Arginine (0.824–1.147), Proline (1.114–1.684), and Tryptophan (0.063–0.098). In conclusion, this study constructed a dynamic model for the protein and amino acid requirements of Jing Tint 6 chicks during the brooding period, providing an important insight to improve precise feeding for layer chicks through this dynamic model calculation. Full article
(This article belongs to the Special Issue Amino Acid Nutrition in Poultry)
30 pages, 2252 KiB  
Review
Understanding Circular RNAs in Health, Welfare, and Productive Traits of Cattle, Goats, and Sheep
by Dimitra Kirgiafini, Maria-Anna Kyrgiafini, Theocharis Gournaris and Zissis Mamuris
Animals 2024, 14(5), 733; https://doi.org/10.3390/ani14050733 - 27 Feb 2024
Cited by 8 | Viewed by 2928
Abstract
Circular RNAs (circRNAs) are unique noncoding RNA molecules, notable for their covalent closed-loop structures, which play a crucial role in regulating gene expression across a variety of biological processes. This review comprehensively synthesizes the existing knowledge of circRNAs in three key livestock species: [...] Read more.
Circular RNAs (circRNAs) are unique noncoding RNA molecules, notable for their covalent closed-loop structures, which play a crucial role in regulating gene expression across a variety of biological processes. This review comprehensively synthesizes the existing knowledge of circRNAs in three key livestock species: Bos taurus (cattle), Ovis aries (sheep), and Capra hircus (goats). It focuses on their functional importance and emerging potential as biomarkers for disease detection, stress response, and overall physiological health. Specifically, it delves into the expression and functionality of circRNAs in these species, paying special attention to traits critical to livestock productivity such as milk production, meat quality, muscle development, wool production, immune responses, etc. We also address the current challenges faced in circRNA research, including the need for standardized methodologies and broader studies. By providing insights into the molecular mechanisms regulated by circRNAs, this review underscores their scientific and economic relevance in the livestock industry. The potential of circRNAs to improve animal health management and the quality of animal-derived products aligns with growing consumer concerns for animal welfare and sustainability. Thus, this paper aims to guide future research directions while supporting the development of innovative strategies in livestock management and breeding. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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24 pages, 319 KiB  
Article
A Reexamination of the Relationship between Training Practices and Welfare in the Management of Ambassador Animals
by Steve Martin, Grey Stafford and David S. Miller
Animals 2024, 14(5), 736; https://doi.org/10.3390/ani14050736 - 27 Feb 2024
Cited by 2 | Viewed by 5597
Abstract
There is an ethical need to document and develop best practices for meeting ambassador animals’ welfare needs within the context of meeting zoo and aquarium program objectives. This is because ambassador animals experience direct and frequent contact with humans. This paper rigorously synthesizes [...] Read more.
There is an ethical need to document and develop best practices for meeting ambassador animals’ welfare needs within the context of meeting zoo and aquarium program objectives. This is because ambassador animals experience direct and frequent contact with humans. This paper rigorously synthesizes behavioral research and theory, contemporary practices, and personal experiences to offer key concepts that can be applied to meet ambassador animal welfare needs. These key concepts include addressing an animal’s recognition of choice and control, the use of the most positive and least intrusive effective interventions when training animals to participate in programming, and an overall reduction in aversive strategy use. Our model for increasing ambassador animal welfare focuses on seven main areas of concern, including the following: choosing the most suitable animal for the program; choosing the human with the right skills and knowledge for the program; using the most positive, least intrusive, effective training methods; developing a strong trusting relationship between trainer and animal; developing a comprehensive enrichment program; the need for institutional support; and creating opportunities for animals to practice species-appropriate behaviors. Our model will provide guidelines for improved ambassador animal welfare that can be refined with future research. Full article
(This article belongs to the Special Issue Zoo and Aquarium Welfare, Ethics, Behavior)
23 pages, 3187 KiB  
Article
Dietary Protein Quality Affects the Interplay between Gut Microbiota and Host Performance in Nile Tilapia
by Gabriella do Vale Pereira, Carla Teixeira, José Couto, Jorge Dias, Paulo Rema and Ana Teresa Gonçalves
Animals 2024, 14(5), 714; https://doi.org/10.3390/ani14050714 - 24 Feb 2024
Cited by 7 | Viewed by 3254
Abstract
Dietary protein quality plays a key role in maintaining intestinal mucosal integrity, but also modulates the growth of luminal microorganisms. This work assessed the effect of dietary protein sources on the performance, gut morphology, and microbiome in Nile tilapia. Four isonitrogenous and isolipidic [...] Read more.
Dietary protein quality plays a key role in maintaining intestinal mucosal integrity, but also modulates the growth of luminal microorganisms. This work assessed the effect of dietary protein sources on the performance, gut morphology, and microbiome in Nile tilapia. Four isonitrogenous and isolipidic diets comprising equivalent amounts of the protein supply derived from either PLANT, ANIMAL, INSECT, or BACTERIAL (bacterial biomass) sources were fed to triplicate groups of fish (IBW: 12 g) for 46 days. Fish fed the ANIMAL and BACTERIAL diets showed significantly higher weight gains than those fed the PLANT and INSECT diets (p < 0.05). Relative abundance at the phylum level showed that Bacteroidetes, Fusobacteria, and Proteobacteria were the more abundant phyla in tilapia’s intestine, while Cetobacterium was the most representative genus in all treatments. Interesting patterns were observed in the correlation between amino acid intake and genus and species abundance. Metabolism prediction analysis showed that BACTERIAL amine and polyamine degradation pathways are modulated depending on diets. In conclusion, different protein sources modulate the relationship between bacteria functional pathways and amino acid intake. Full article
(This article belongs to the Special Issue Feed Ingredients and Fish Mucosal Health)
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32 pages, 1417 KiB  
Article
Dolphin-WET—Development of a Welfare Evaluation Tool for Bottlenose Dolphins (Tursiops truncatus) under Human Care
by Katrin Baumgartner, Tim Hüttner, Isabella L. K. Clegg, Manuel Garcia Hartmann, Daniel Garcia-Párraga, Xavier Manteca, Birgitta Mercera, Tania Monreal-Pawlowsky, Cristina Pilenga, Kerstin Ternes, Oriol Tallo-Parra, Ruta Vaicekauskaite, Lorenzo von Fersen, Lisa Yon and Fabienne Delfour
Animals 2024, 14(5), 701; https://doi.org/10.3390/ani14050701 - 23 Feb 2024
Cited by 3 | Viewed by 5710
Abstract
Ensuring high standards of animal welfare is not only an ethical duty for zoos and aquariums, but it is also essential to achieve their conservation, education, and research goals. While for some species, animal welfare assessment frameworks are already in place, little has [...] Read more.
Ensuring high standards of animal welfare is not only an ethical duty for zoos and aquariums, but it is also essential to achieve their conservation, education, and research goals. While for some species, animal welfare assessment frameworks are already in place, little has been done for marine animals under human care. Responding to this demand, the welfare committee of the European Association for Aquatic Mammals (EAAM) set up a group of experts on welfare science, cetacean biology, and zoo animal medicine across Europe. Their objective was to develop a comprehensive tool to evaluate the welfare of bottlenose dolphins (Tursiops truncatus), named Dolphin-WET. The tool encompasses 49 indicators that were either validated through peer review or management-based expertise. The first of its kind, the Dolphin-WET is a species-specific welfare assessment tool that provides a holistic approach to evaluating dolphin welfare. Inspired by Mellor’s Five Domains Model and the Welfare Quality®, its hierarchical structure allows for detailed assessments from overall welfare down to specific indicators. Through combining 37 animal-based and 12 resource-based indicators that are evaluated based on a two- or three-level scoring, the protocol offers a detailed evaluation of individual dolphins. This approach allows for regular internal monitoring and targeted welfare management, enabling caretakers to address specific welfare concerns effectively. Full article
(This article belongs to the Special Issue Advances in Marine Mammal Cognition and Cognitive Welfare)
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31 pages, 13259 KiB  
Article
Impact of Climate Change on the Distribution of Three Rare Salamanders (Liua shihi, Pseudohynobius jinfo, and Tylototriton wenxianensis) in Chongqing, China, and Their Conservation Implications
by Qi Ma, Lipeng Wan, Shengchao Shi and Zhijian Wang
Animals 2024, 14(5), 672; https://doi.org/10.3390/ani14050672 - 21 Feb 2024
Cited by 9 | Viewed by 2429
Abstract
The Wushan Salamander (Liua shihi), Jinfo Salamander (Pseudohynobius jinfo), and Wenxian Knobby Salamander (Tylototriton wenxianensis) are rare national Class II protected wild animals in China. We performed MaxEnt modeling to predict and analyze the potential distribution and [...] Read more.
The Wushan Salamander (Liua shihi), Jinfo Salamander (Pseudohynobius jinfo), and Wenxian Knobby Salamander (Tylototriton wenxianensis) are rare national Class II protected wild animals in China. We performed MaxEnt modeling to predict and analyze the potential distribution and trends of these species in Chongqing under current and future climate conditions. Species distribution data were primarily obtained from field surveys, supplemented by museum collections and the existing literature. These efforts yielded 636 records, including 43 for P. jinfo, 23 for T. wenxianensis, and 570 for L. shihi. Duplicate records within the same 100 m × 100 m grid cell were removed using ENMTools, resulting in 10, 12, and 58 valid distribution points for P. jinfo, T. wenxianensis, and L. shihi, respectively. The optimization of feature class parameters (FC) and the regularization multiplier (RM) were applied using R package “ENMeval 2.0” to establish the optimal model with MaxEnt. The refined models were applied to simulate the suitable distribution areas for the three species. The results indicate that the current suitable habitat area for L. shihi accounted for 9.72% of the whole region of the Chongqing municipality. It is projected that, by 2050, the proportion of suitable habitat will increase to 12.54% but will decrease to 11.98% by 2070 and further decline to 8.80% by 2090. The current suitable habitat area for P. jinfo accounted for 1.08% of the whole region of the Chongqing municipality, which is expected to decrease to 0.31%% by 2050, 0.20% by 2070, and 0.07% by 2090. The current suitable habitat area for T. wenxianensis accounted for 0.81% of the whole region of the Chongqing municipality, which is anticipated to decrease to 0.37% by 2050, 0.21% by 2070, and 0.06% by 2090. Human disturbance, climate variables, and habitat characteristics are the primary factors influencing the distribution of three salamander species in Chongqing. The proximity to roads significantly impacts L. shihi, while climate conditions mainly affect P. jinfo, and the distance to water sources is crucial for T. wenxianensis. The following suggestions were made based on key variables identified for each species: (1) For L. shihi, it is imperative to minimize human disturbances and preserve areas without roads and the existing vegetation within nature reserves to ensure their continued existence. (2) For P. jinfo, the conservation of high-altitude habitats is of utmost importance, along with the reduction in disturbances caused by roads to maintain the species’ ecological niche. (3) For T. wenxianensis, the protection of aquatic habitats is crucial. Additionally, efforts to mitigate the impacts of road construction and enhance public awareness are essential for the preservation of this species and the connectivity of its habitats. Full article
(This article belongs to the Special Issue Adaptive Responses of Vertebrates to Climate Change)
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19 pages, 9714 KiB  
Article
Exploration of Toxins from a Marine Annelid: An Analysis of Phyllotoxins and Accompanying Bioactives
by Ana P. Rodrigo, Inês Moutinho Cabral, António Alexandre and Pedro M. Costa
Animals 2024, 14(4), 635; https://doi.org/10.3390/ani14040635 - 16 Feb 2024
Cited by 1 | Viewed by 3204
Abstract
Proteinaceous toxins are peptides or proteins that hold great biotechnological value, evidenced by their ecological role, whether as defense or predation mechanisms. Bioprospecting using bioinformatics and omics may render screening for novel bioactives more expeditious, especially considering the immense diversity of toxin-secreting marine [...] Read more.
Proteinaceous toxins are peptides or proteins that hold great biotechnological value, evidenced by their ecological role, whether as defense or predation mechanisms. Bioprospecting using bioinformatics and omics may render screening for novel bioactives more expeditious, especially considering the immense diversity of toxin-secreting marine organisms. Eulalia sp. (Annelida: Phyllodocidae), a toxin bearing marine annelid, was recently shown to secrete cysteine-rich protein (Crisp) toxins (hitherto referred to as ‘phyllotoxins’) that can immobilize its prey. By analyzing and validating transcriptomic data, we narrowed the list of isolated full coding sequences of transcripts of the most abundant toxins or accompanying bioactives secreted by the species (the phyllotoxin Crisp, hyaluronidase, serine protease, and peptidases M12A, M13, and M12B). Through homology matching with human proteins, the biotechnological potential of the marine annelid’s toxins and related proteins was tentatively associated with coagulative and anti-inflammatory responses for the peptidases PepM12A, SePr, PepM12B, and PepM13, and with the neurotoxic activity of Crisp, and finally, hyaluronidase was inferred to bear properties of an permeabilizing agent. The in silico analysis succeeded by validation by PCR and Sanger sequencing enabled us to retrieve cDNAs can may be used for the heterologous expression of these toxins. Full article
(This article belongs to the Special Issue Marine Invertebrates: From Ecological Traits to Biotechnological Clues)
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15 pages, 320 KiB  
Review
Genetic Parameter Estimation and Selection for Resistance to Gastrointestinal Nematode Parasites in Sheep—A Review
by Samla Marques Freire Cunha, Olivia Willoughby, Flavio Schenkel and Ángela Cánovas
Animals 2024, 14(4), 613; https://doi.org/10.3390/ani14040613 - 14 Feb 2024
Cited by 6 | Viewed by 3136
Abstract
Gastrointestinal nematodes (GINs) are a major problem affecting sheep production systems worldwide. The flocks infected with GINs can undergo significant economic losses due to a decrease in productivity, the animals’ deaths, and the costs associated with treatments. The over-reliance on anthelmintics in the [...] Read more.
Gastrointestinal nematodes (GINs) are a major problem affecting sheep production systems worldwide. The flocks infected with GINs can undergo significant economic losses due to a decrease in productivity, the animals’ deaths, and the costs associated with treatments. The over-reliance on anthelmintics in the past years to eliminate GINs has resulted in the development of resistance against the available commercial anthelmintics. Genetically resistant animals can be used in mating systems to improve the overall flock resistance. This review aimed to summarize the estimated genetic parameters for resistance traits and genetic gains through the use of genetic/genomic selection for resistance to GINs in sheep. Heritability estimates from the literature ranged from 0.00 to 0.46 for fecal egg counts, 0.12 to 0.37 for packed cell volume/hematocrit, 0.07 to 0.26 for FAffa MAlan CHArt (FAMACHA©), from 0.10 to 0.37 for blood parameters, and 0.19 for Immunoglobulin A. Genetic correlations between traits measuring resistance to GINs and production traits ranged from negative to positive values in the literature. Genetic gains are possible when genetic/genomic selection for GIN resistance is applied. Therefore, genetic/genomic selection can be used to improve flocks’ resistance to GINs as a sustainable approach in sheep production systems. Full article
(This article belongs to the Special Issue Second Edition of Breeding for Disease Resistance in Ruminants)
29 pages, 7110 KiB  
Systematic Review
A Review of Potential Feed Additives Intended for Carbon Footprint Reduction through Methane Abatement in Dairy Cattle
by Ian Hodge, Patrick Quille and Shane O’Connell
Animals 2024, 14(4), 568; https://doi.org/10.3390/ani14040568 - 8 Feb 2024
Cited by 11 | Viewed by 6423
Abstract
Eight rumen additives were chosen for an enteric methane-mitigating comparison study including garlic oil (GO), nitrate, Ascophyllum nodosum (AN), Asparagopsis (ASP), Lactobacillus plantarum (LAB), chitosan (CHI), essential oils (EOs) and 3-nitrooxypropanol (3-NOP). Dose-dependent analysis was carried out on selected feed additives using a [...] Read more.
Eight rumen additives were chosen for an enteric methane-mitigating comparison study including garlic oil (GO), nitrate, Ascophyllum nodosum (AN), Asparagopsis (ASP), Lactobacillus plantarum (LAB), chitosan (CHI), essential oils (EOs) and 3-nitrooxypropanol (3-NOP). Dose-dependent analysis was carried out on selected feed additives using a meta-analysis approach to determine effectiveness in live subjects or potential efficacy in live animal trials with particular attention given to enteric gas, volatile fatty acid concentrations, and rumen microbial counts. All meta-analysis involving additives GO, nitrates, LAB, CHI, EOs, and 3-NOP revealed a reduction in methane production, while individual studies for AN and ASP displayed ruminal bacterial community improvement and a reduction in enteric CH4. Rumen protozoal depression was observed with GO and AN supplementation as well as an increase in propionate production with GO, LAB, ASP, CHI, and 3-NOP rumen fluid inoculation. GO, AN, ASP, and LAB demonstrated mechanisms in vitro as feed additives to improve rumen function and act as enteric methane mitigators. Enzyme inhibitor 3-NOP displays the greatest in vivo CH4 mitigating capabilities compared to essential oil commercial products. Furthermore, this meta-analysis study revealed that in vitro studies in general displayed a greater level of methane mitigation with these compounds than was seen in vivo, emphasising the importance of in vivo trials for final verification of use. While in vitro gas production systems predict in vivo methane production and fermentation trends with reasonable accuracy, it is necessary to confirm feed additive rumen influence in vivo before practical application. Full article
(This article belongs to the Special Issue Nutritional Strategies to Control Enteric Methane Production of Ruminants)
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23 pages, 2057 KiB  
Article
Alternatives to Carbon Dioxide in Two Phases for the Improvement of Broiler Chickens’ Welfare during Stunning
by Daniel Santiago Rucinque, Antonio Velarde, Aida Xercavins, Aranzazu Varvaró-Porter, Troy John Gibson, Virginie Michel and Alexandra Contreras-Jodar
Animals 2024, 14(3), 486; https://doi.org/10.3390/ani14030486 - 1 Feb 2024
Viewed by 3251
Abstract
This study evaluated the exposure to gas mixtures of carbon dioxide (CO2) associated with nitrogen (N2) as alternatives to CO2 in two phases to improve the welfare of broiler chickens at slaughter. Broilers were exposed to one of [...] Read more.
This study evaluated the exposure to gas mixtures of carbon dioxide (CO2) associated with nitrogen (N2) as alternatives to CO2 in two phases to improve the welfare of broiler chickens at slaughter. Broilers were exposed to one of three treatments: 40C90C (1st phase: <40% CO2 for 2 min; 2nd phase: >90% CO2 and <2% O2 for 2 min, n = 92), 40C60N (40% CO2, 60% N2, and <2% O2 for 4 min, n = 79), or 20C80N (20% CO2, 80% N2, and <2% O2 for 4 min, n = 72). Brain activity (EEG) was assessed to determine the onset of loss of consciousness (LOC) and death. Behavioural assessment allowed for characterisation of an aversive response to the treatments and confirmed loss of posture (LOP) and motionlessness as behavioural proxies of LOC and brain death in 40C60N and 20N80C. However, the lack of quality of the EEG traces obtained in 40C90C did not allow us to determine the onset of LOC and brain death for this treatment. The onset of LOC in 40C60N was found at 19 s [14–30 s] and in 20C80N at 21 s [16–37 s], whereas a LOP was seen at 53 s [26–156 s] in 40C90C. Birds showed brain death in 40C60N at 64 s [43–108 s] and in 20C80N at 70 s [45–88 s]), while they became motionless in 40C90C at 177 s [89–212 s]. The 40C90C birds not only experienced more events of aversive behaviours related to mucosal irritation, dyspnoea, and breathlessness during induction to unconsciousness but were at risk of remaining conscious when the CO2 concentration was increased in the 2nd phase (known to cause severe pain). From an animal welfare point of view, 40C60N proved to be the least aversive of the three treatments tested, followed by 20C80N and 40C90C. Full article
(This article belongs to the Special Issue Animal Stress and Welfare During Transport and Slaughtering)
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11 pages, 250 KiB  
Article
Effects of Dietary Crude Protein Level of Concentrate Mix on Growth Performance, Rumen Characteristics, Blood Metabolites, and Methane Emissions in Fattening Hanwoo Steers
by Joonpyo Oh, Hyunjin Cho, Sinyong Jeong, Kyewon Kang, Mingyung Lee, Seoyoung Jeon, Hamin Kang and Seongwon Seo
Animals 2024, 14(3), 469; https://doi.org/10.3390/ani14030469 - 31 Jan 2024
Cited by 3 | Viewed by 2192
Abstract
This study aimed to investigate the effect of varying levels of dietary crude protein (CP) on growth performance, rumen characteristics, blood metabolites, and methane emissions in fattening Hanwoo steers. Twenty-four steers, weighing 504 ± 33.0 kg (16 months old), were assigned to four [...] Read more.
This study aimed to investigate the effect of varying levels of dietary crude protein (CP) on growth performance, rumen characteristics, blood metabolites, and methane emissions in fattening Hanwoo steers. Twenty-four steers, weighing 504 ± 33.0 kg (16 months old), were assigned to four dietary treatments with different CP concentrations (15, 18, 19, and 21% of CP on a dry matter (DM) basis). A linear increasing trend in the average daily gain (ADG) was observed (p = 0.066). With increased dietary CP levels, the rumen ammonia concentration significantly increased (p < 0.001), while the propionate proportion linearly decreased (p = 0.004) and the proportions of butyrate and valerate linearly increased (p ≤ 0.003). The blood urea exhibited a linear increase (p < 0.001), whereas the blood non-esterified fatty acids and cholesterol showed a linear decrease (p ≤ 0.003) with increasing dietary CP. The methane concentration from eructation per intake (ppm/kg), forage neutral detergent fiber (NDF) intake, total NDF intake, and ADG exhibited linear decreases (p ≤ 0.014) across the treatments. In conclusion, increasing the dietary CP up to 21% in concentrates demonstrated a tendency to linearly increase the ADG and significantly decrease the propionate while increasing the butyrate. The methane concentration from eructation exhibited a tendency to linearly decrease with increasing dietary CP. Full article
(This article belongs to the Section Animal Nutrition)
13 pages, 2622 KiB  
Technical Note
Computed Tomography Evaluation of Frozen or Glycerinated Bradypus variegatus Cadavers: A Comprehensive View with Emphasis on Anatomical Aspects
by Michel Santos e Cunha, Rodrigo dos Santos Albuquerque, José Gonçalo Monteiro Campos, Francisco Décio de Oliveira Monteiro, Kayan da Cunha Rossy, Thiago da Silva Cardoso, Lucas Santos Carvalho, Luisa Pucci Bueno Borges, Sheyla Farhayldes Souza Domingues, Roberto Thiesen, Roberta Martins Crivelaro Thiesen and Pedro Paulo Maia Teixeira
Animals 2024, 14(3), 355; https://doi.org/10.3390/ani14030355 - 23 Jan 2024
Cited by 1 | Viewed by 1911
Abstract
Bradypus variegatus has unique anatomical characteristics, and many of its vascular and digestive tract aspects have yet to be clearly understood. This lack of information makes clinical diagnoses and surgical procedures difficult. The aim of this study was to evaluate the anatomical aspects [...] Read more.
Bradypus variegatus has unique anatomical characteristics, and many of its vascular and digestive tract aspects have yet to be clearly understood. This lack of information makes clinical diagnoses and surgical procedures difficult. The aim of this study was to evaluate the anatomical aspects of frozen and glycerinated corpses of B. variegatus using computed tomography (CT), emphasizing vascular and digestive contrast studies. Nine corpses that died during routine hospital were examined via CT in the supine position with scanning in the craniocaudal direction. In frozen cadavers, the contrast was injected into a cephalic vein after thawing and, subsequently, was administered orally. In addition to bone structures, CT allowed the identification of organs, soft tissues, and vascular structures in specimens. Visualization of soft tissues was better after contrast been administered intravenously and orally, even without active vascularization. Furthermore, the surfaces of the organs were highlighted by the glycerination method. With this technique, it was possible to describe part of the vascularization of the brachial, cervical, thoracic, and abdominal regions, in addition to highlighting the esophagus and part of the stomach. CT can be another tool for the evaluation of B. variegatus cadavers by anatomists or pathologists, contributing to the identification of anatomical structures. Full article
(This article belongs to the Section Mammals)
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44 pages, 4601 KiB  
Article
Impacts of Puppy Early Life Experiences, Puppy-Purchasing Practices, and Owner Characteristics on Owner-Reported Problem Behaviours in a UK Pandemic Puppies Cohort at 21 Months of Age
by Claire L. Brand, Dan G. O’Neill, Zoe Belshaw, Fiona C. Dale, Bree L. Merritt, Kathryn N. Clover, Mi-Xue Michelle Tay, Camilla L. Pegram and Rowena M. A. Packer
Animals 2024, 14(2), 336; https://doi.org/10.3390/ani14020336 - 22 Jan 2024
Cited by 11 | Viewed by 8758
Abstract
Problem behaviours are a leading cause of relinquishment and euthanasia of young dogs. Previous research has identified associations between owner-reported problem behaviours and risk factors, including how dogs were acquired as puppies, early socialisation experiences, and owners’ experience levels. Puppies acquired during the [...] Read more.
Problem behaviours are a leading cause of relinquishment and euthanasia of young dogs. Previous research has identified associations between owner-reported problem behaviours and risk factors, including how dogs were acquired as puppies, early socialisation experiences, and owners’ experience levels. Puppies acquired during the 2020 phase of the UK COVID-19 pandemic (“Pandemic Puppies”) were more likely to be exposed to many of these risk factors compared to puppies acquired in 2019; however, consequences for their adult behaviour are, as yet, unexplored. This study aimed to investigate the impact of these early-life and provenance-based risk factors, in addition to owner management strategies (including training methods) into early adulthood, upon adult dog behaviour aged 21 months. An online longitudinal cohort study of n = 985 Pandemic Puppies was conducted, recruited from a cohort of n = 4369 puppies originally surveyed in November–December 2020, which included data on how and why they were acquired and their socialisation/habituation experiences <16 weeks of age. Nearly all owners (96.7%) reported that their dog had exhibited at least one problem behaviour up to 21 months of age (median: 5; IQR: 3–7). Almost one third of dogs (30.9%) were reported to have displayed separation-related behaviours. Multivariable linear regression modelling revealed that owners reporting more problem behaviours were more likely to use multiple aversive training techniques (e.g., physical punishment), the use of which was notably high in this population (82.3%) compared to previous studies. Attendance at online puppy classes was the sole protective factor against owner use of aversive training methods. Almost one third of owners had underestimated how hard training their dog would be; this view was more likely among first-time owners. Urgent efforts are required to support this vulnerable population of dogs, including providing owner education regarding humane training techniques, to improve canine emotional health and avoid future relinquishment and/or behavioural euthanasia. Full article
(This article belongs to the Section Companion Animals)
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12 pages, 1382 KiB  
Article
An Influx of Non-Native Bird Species into the Natural Environment Owing to the Accidental Release of Pet Birds in Japan
by Sumiko Nishida and Wataru Kitamura
Animals 2024, 14(2), 221; https://doi.org/10.3390/ani14020221 - 10 Jan 2024
Cited by 4 | Viewed by 4015
Abstract
The escape of pet birds into the wild raises concerns about the introduction of invasive avian species. This study investigated the impact of escaped pet birds on the introduction of non-native species in Japan. Data sourced from four lost-and-found pet websites between January [...] Read more.
The escape of pet birds into the wild raises concerns about the introduction of invasive avian species. This study investigated the impact of escaped pet birds on the introduction of non-native species in Japan. Data sourced from four lost-and-found pet websites between January 2018 and December 2021 revealed 12,125 recorded escapes exhibiting both daily occurrences and seasonal fluctuations. Statistical modeling identified the monthly average temperature (positively correlated) and maximum electricity demand (negatively correlated) as influential factors. Text analysis revealed “window” and “open” as frequently cited reasons for escapes. Budgerigars (Melopsittacus undulatus) and Cockatiels (Nymphicus hollandicus) accounted for 76% of the total escape, suggesting a low perceived risk of establishment in nonnative environments. Interestingly, two globally established invasive species, the Rose-ringed Parakeet (Psittacula krameri) and Monk Parakeet (Myiopsitta monachus), were among the escaped birds. While the Rose-ringed Parakeet is locally naturalized in Tokyo and its adjacent prefectures, the Monk Parakeet failed to establish itself in Japan. Despite the limited number of escaped Monk Parakeets, ongoing efforts are crucial for preventing the potential re-establishment of species with such capabilities. Full article
(This article belongs to the Special Issue Invasive Birds: New Advances and Perspectives)
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14 pages, 257 KiB  
Article
Effect of a Dietary Essential Oil Blend in Dairy Cows during the Dry and Transition Period on Blood and Metabolic Parameters of Dams and Their Calves
by Cangir Uyarlar, Abdur Rahman, Eyup Eren Gultepe, Ibrahim Sadi Cetingul and Ismail Bayram
Animals 2024, 14(1), 150; https://doi.org/10.3390/ani14010150 - 2 Jan 2024
Cited by 5 | Viewed by 2889
Abstract
Cows face severe challenges of immunosuppression and negative energy balance during transition periods. The current study was designed to investigate the effects of essential oil blend supplementation in dairy cow’s ration during dry periods on the health of the cow and calf. In [...] Read more.
Cows face severe challenges of immunosuppression and negative energy balance during transition periods. The current study was designed to investigate the effects of essential oil blend supplementation in dairy cow’s ration during dry periods on the health of the cow and calf. In the current study, 45 dry Holstein dairy cows were divided into three groups, each consisting of 15 animals. The control group was without any supplementation; the positive control group was only injected with 0.2 mg/kg levamisole (intramuscular) at 2 months before parturition and 1 month before parturition; and the treatment group was supplemented with 3 g/day for each cow essential oil blend mixed in total mixed ration (TMR). A mixed vaccine against E. coli, Rotavirus, and Coronavirus was administered to all cows before 42 days and after 21 days of calving. The day of the calving of the animal was accepted as day 0. Blood samples were collected from the coccygeal vein of all cows at −60, −45, −30, −15, −7, 0, 1 and 2 days, and the colostrum samples were taken on days 0, 1, and 2. Blood samples were also collected from the jugular vein (V. Jugularis) of the newborn calves on days 0, 1, and 2. The results of the hematological parameters revealed no difference in the total red blood cell count, hemoglobin amount, hematocrit, MCV, MCH, MCHC, RDW, PLT, MPV, and PCT values in both prenatal and postnatal blood of the cows (p > 0.05). In terms of immunological parameters, the total IgG level was significantly higher in the experimental group on the 7th day before birth compared to the other two groups, while the total leukocyte count, lymphocyte count, monocyte count and granulocyte counts were found to be lower after birth (p < 0005). Blood NEFA and BHBA levels were also lower in the experimental group compared to the other two groups (p < 0.005), and the blood glucose level was not different (p > 0.05). In calves, no difference was found between the groups in any of the parameters examined (p > 0.05). It is concluded that the dietary addition of an essential oil blend during the transition period enhanced the immune status and energy metabolism of cows without any effect on the health status of newborn calves. Full article
(This article belongs to the Section Animal Physiology)
12 pages, 3766 KiB  
Article
Cultivable Gut Microbiota in Synanthropic Bats: Shifts of Its Composition and Diversity Associated with Hibernation
by Igor V. Popov, Iraida S. Berezinskaia, Ilia V. Popov, Irina B. Martiusheva, Elizaveta V. Tkacheva, Vladislav E. Gorobets, Iuliia A. Tikhmeneva, Anna V. Aleshukina, Tatiana I. Tverdokhlebova, Michael L. Chikindas, Koen Venema and Alexey M. Ermakov
Animals 2023, 13(23), 3658; https://doi.org/10.3390/ani13233658 - 26 Nov 2023
Cited by 8 | Viewed by 2796
Abstract
The role of bats in the global microbial ecology no doubt is significant due to their unique immune responses, ability to fly, and long lifespan, all contributing to pathogen spread. Some of these animals hibernate during winter, which results in the altering of [...] Read more.
The role of bats in the global microbial ecology no doubt is significant due to their unique immune responses, ability to fly, and long lifespan, all contributing to pathogen spread. Some of these animals hibernate during winter, which results in the altering of their physiology. However, gut microbiota shifts during hibernation is little studied. In this research, we studied cultivable gut microbiota composition and diversity of Nyctalus noctula before, during, and after hibernation in a bat rehabilitation center. Gut microorganisms were isolated on a broad spectrum of culture media, counted, and identified with mass spectrometry. Linear modeling was used to investigate associations between microorganism abundance and N. noctula physiological status, and alpha- and beta-diversity indexes were used to explore diversity changes. As a result, most notable changes were observed in Serratia liquefaciens, Hafnia alvei, Staphylococcus sciuri, and Staphylococcus xylosus, which were significantly more highly abundant in hibernating bats, while Citrobacter freundii, Klebsiella oxytoca, Providencia rettgeri, Citrobacter braakii, and Pedicoccus pentosaceus were more abundant in active bats before hibernation. The alpha-diversity was the lowest in hibernating bats, while the beta-diversity differed significantly among all studied periods. Overall, this study shows that hibernation contributes to changes in bat cultivable gut microbiota composition and diversity. Full article
(This article belongs to the Special Issue Bats of Europe: Current Research on Diversity, Ecology and Conservation Needs)
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18 pages, 2195 KiB  
Article
Understanding the Factors Influencing Cat Containment: Identifying Opportunities for Behaviour Change
by Gemma C. Ma and Lynette J. McLeod
Animals 2023, 13(10), 1630; https://doi.org/10.3390/ani13101630 - 12 May 2023
Cited by 11 | Viewed by 3917
Abstract
There are over 5 million pet cats in Australia managed on a spectrum from fully indoors to completely outdoor free roaming. Roaming cats threaten biodiversity, can create a nuisance and are at risk of accidents and injury. Hence, there is substantial interest in [...] Read more.
There are over 5 million pet cats in Australia managed on a spectrum from fully indoors to completely outdoor free roaming. Roaming cats threaten biodiversity, can create a nuisance and are at risk of accidents and injury. Hence, there is substantial interest in behaviour change interventions to increase cat containment. An online questionnaire collected information on cat owner demographics, the number of cats owned, current containment behaviours and an agreement with 15 capability, opportunity and motivation (COM) items. Responses were received from 4482 cat owners. More than half (65%) indicated that they currently keep their cat(s) fully contained. Another 24% practiced a night curfew. Owners’ psychological capability had the greatest influence on containment behaviour. Motivation (community- and cat welfare-framed), living in an apartment and renting were also associated with a greater likelihood of containment. Cat owners not currently containing their cats could be divided into six profiles who differed on agreement with COM themes, age, future intentions, current behaviour, location and gender. Understanding differences between cat owner segments can assist with designing behaviour change interventions. Increasing cat owners’ psychological capability to contain their cats and encouraging the adoption of a night curfew as a first step towards 24 h containment are recommended. Full article
(This article belongs to the Section Companion Animals)
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12 pages, 872 KiB  
Article
Multimodal Communication in the Human–Cat Relationship: A Pilot Study
by Charlotte de Mouzon and Gérard Leboucher
Animals 2023, 13(9), 1528; https://doi.org/10.3390/ani13091528 - 3 May 2023
Cited by 7 | Viewed by 21234
Abstract
Across all species, communication implies that an emitter sends signals to a receiver, through one or more channels. Cats can integrate visual and auditory signals sent by humans and modulate their behaviour according to the valence of the emotion perceived. However, the specific [...] Read more.
Across all species, communication implies that an emitter sends signals to a receiver, through one or more channels. Cats can integrate visual and auditory signals sent by humans and modulate their behaviour according to the valence of the emotion perceived. However, the specific patterns and channels governing cat-to-human communication are poorly understood. This study addresses whether, in an extraspecific interaction, cats are sensitive to the communication channel used by their human interlocutor. We examined three types of interactions—vocal, visual, and bimodal—by coding video clips of 12 cats living in cat cafés. In a fourth (control) condition, the human interlocutor refrained from emitting any communication signal. We found that the modality of communication had a significant effect on the latency in the time taken for cats to approach the human experimenter. Cats interacted significantly faster to visual and bimodal communication compared to the “no communication” pattern, as well as to vocal communication. In addition, communication modality had a significant effect on tail-wagging behaviour. Cats displayed significantly more tail wagging when the experimenter engaged in no communication (control condition) compared to visual and bimodal communication modes, indicating that they were less comfortable in this control condition. Cats also displayed more tail wagging in response to vocal communication compared to the bimodal communication. Overall, our data suggest that cats display a marked preference for both visual and bimodal cues addressed by non-familiar humans compared to vocal cues only. Results arising from the present study may serve as a basis for practical recommendations to navigate the codes of human–cat interactions. Full article
(This article belongs to the Section Companion Animals)
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23 pages, 5672 KiB  
Article
Taurine Supplementation to Plant-Based Diets Improves Lipid Metabolism in Senegalese Sole
by Cláudia Aragão, Rita Teodósio, Rita Colen, Nadège Richard, Ivar Rønnestad, Jorge Dias, Luís E. C. Conceição and Laura Ribeiro
Animals 2023, 13(9), 1501; https://doi.org/10.3390/ani13091501 - 28 Apr 2023
Cited by 7 | Viewed by 4865
Abstract
Taurine is a sulphur-containing amino acid with important physiological roles and a key compound for the synthesis of bile salts, which are essential for the emulsion and absorption of dietary lipids. This study aimed to evaluate the effects of taurine supplementation to low-fishmeal [...] Read more.
Taurine is a sulphur-containing amino acid with important physiological roles and a key compound for the synthesis of bile salts, which are essential for the emulsion and absorption of dietary lipids. This study aimed to evaluate the effects of taurine supplementation to low-fishmeal diets on the metabolism of taurine, bile acids, and lipids of Senegalese sole. A fishmeal (FM) and a plant-protein-based (PP0) diet were formulated, and the latter was supplemented with taurine at 0.5 and 1.5% (diets PP0.5 and PP1.5). Diets were assigned to triplicate tanks containing 35 fish (initial weight ~14 g) for 6 weeks. Fish from the PP0 treatment presented lower taurine and bile-acid concentrations compared with the FM treatment, and a downregulation of cyp7a1 and abcb11 was observed. Triolein catabolism decreased in PP0-fed fish, resulting in increased hepatic fat content and plasma triglycerides, while no effects on plasma cholesterol were observed. Taurine supplementation to plant-based diets resulted in a higher taurine accumulation in fish tissues, increased bile-acid concentration, and upregulation of cyp7a1 and abcb11. Hepatic fat content and plasma triglycerides decreased with increasing dietary taurine supplementation. Taurine supplementation mitigated part of the negative effects of plant-based diets, leading to better lipid utilisation. Full article
(This article belongs to the Special Issue Amino Acid Supplementation in Fish Nutrition and Welfare)
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17 pages, 2294 KiB  
Article
Exploring the Effect of Functional Diets Containing Phytobiotic Compounds in Whiteleg Shrimp Health: Resistance to Acute Hepatopancreatic Necrotic Disease Caused by Vibrio parahaemolyticus
by Carla Hernández-Cabanyero, Esther Carrascosa, Silvia Jiménez and Belén Fouz
Animals 2023, 13(8), 1354; https://doi.org/10.3390/ani13081354 - 15 Apr 2023
Cited by 11 | Viewed by 5138
Abstract
Acute hepatopancreatic necrosis (AHPND) is an emerging severe disease caused by strains of Vibrio parahaemolyticus (VpAHPND) in whiteleg shrimp (Litopenaeus vannamei). Mitigating its negative impact, and at the same time minimizing antibiotics treatments, is the major challenge in [...] Read more.
Acute hepatopancreatic necrosis (AHPND) is an emerging severe disease caused by strains of Vibrio parahaemolyticus (VpAHPND) in whiteleg shrimp (Litopenaeus vannamei). Mitigating its negative impact, and at the same time minimizing antibiotics treatments, is the major challenge in shrimp aquaculture. A sustainable strategy could be to include immunostimulants in diet. Phytobiotics, harmless plant extracts with immunostimulatory and biocidal activities, are promising candidates. In this study, we evaluated the effectiveness of two diets (E and F) supplemented with phytobiotics (functional diets) in terms of protecting shrimp against AHPND. For this purpose, groups of animals were fed functional or control diets for 4 and 5 weeks and, subsequently, they were challenged with VpAHPND by immersion. We compared the mortality in infected groups and estimated the percentage of carriers by using a specific qPCR in hepatopancreas tissue. The results showed that mortality was significantly lower in the group fed functional diet E and, after a 5-week feeding schedule. This group also showed the lowest percentage of carriers. The pathological effects were also reduced with diet F. Thus, feeding shrimp with phytobiotic-enriched diets in critical periods will be highly beneficial because it increases the host’s resistance to AHPND pathology. Full article
(This article belongs to the Special Issue Functional Feeds to Improve Shrimp and Fish Aquaculture)
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16 pages, 4922 KiB  
Article
Red Sea Bream Iridovirus Kinetics, Tissue Tropism, and Interspecies Horizontal Transmission in Flathead Grey Mullets (Mugil cephalus)
by Kyung-Ho Kim, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Mun-Gyeong Kwon, Jae-Ok Kim and Chan-Il Park
Animals 2023, 13(8), 1341; https://doi.org/10.3390/ani13081341 - 13 Apr 2023
Cited by 6 | Viewed by 3166
Abstract
Red sea bream iridovirus (RSIV) causes significant economic losses in the aquaculture industry. We analyzed the pathogenicity of RSIV in flathead grey mullets (Mugil cephalus), the correlation of histopathological lesions, and interspecies horizontal transmission, through immersion infection and cohabitation challenges. Flathead [...] Read more.
Red sea bream iridovirus (RSIV) causes significant economic losses in the aquaculture industry. We analyzed the pathogenicity of RSIV in flathead grey mullets (Mugil cephalus), the correlation of histopathological lesions, and interspecies horizontal transmission, through immersion infection and cohabitation challenges. Flathead grey mullets, which were challenged by immersion infection, exhibited mortality at 14 and 24 days after RSIV exposure. Viral shedding in seawater peaked 2–3 days before or after the observed mortality. Specific lesions of RSIV were observed in the spleen and kidney, and the correlation between histopathological grade and viral load was the highest in the spleen. In a cohabitation challenge, flathead grey mullets were the donors, and healthy rock bream, red sea bream, and flathead grey mullets were the recipients. Viral shedding in seawater was the highest in flathead grey mullet and rock bream at 25 °C, with 106.0 RSIV copies L/g at 14 dpi. No mortality was observed in any group challenged at 15 °C, and no RSIV was detected in seawater after 30 dpi. The virus shed from RSIV-infected flathead grey mullets caused horizontal transmission through seawater. These findings suggest that rapid decision-making is warranted when managing disease in fish farms. Full article
(This article belongs to the Section Aquatic Animals)
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