Molecular Breeding for Wheat Disease Resistance

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Crop Genetics, Genomics and Breeding".

Deadline for manuscript submissions: 5 October 2026 | Viewed by 1161

Special Issue Editor


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Guest Editor
Agricultural Institute, HUN-REN Centre for Agricultural Research, Brunszvik Str. 2, 2462 Martonvasar, Hungary
Interests: resistance breeding; cereal breeding; organic breeding; MAS; detached-leaf technique; leaf pathogenes of wheat; host-pathogen interactions

Special Issue Information

Dear Colleagues,

Wheat is the most important cereal crop of humankind, with the largest current cultivation area. The success of wheat cultivation can significantly influence the situation of plant growers and the national economy. The genetic yield potential of modern, currently cultivated wheat varieties is modified by several factors. However, wheat yields are significantly threatened by several pathogens, which cause an estimated 11.6% annual yield loss in global production. Breeding resistant varieties is the most economical and ecofriendly way to protect against plant diseases.

Advances in molecular breeding, genomics, and crop genetics offer powerful tools to enhance disease resistance. Techniques such as marker-assisted selection, genomic selection, and gene editing enable the precise identification and integration of resistance genes into elite cultivars. Understanding host–pathogen interactions at the molecular level also aids in pyramiding durable resistance genes. Integrating these approaches accelerates the breeding process and supports the development of resilient wheat varieties that can thrive under increasing biotic stresses.

This Special Issue highlights recent progress and future directions in molecular breeding for enhancing wheat disease resistance.

This Special Issue will aim to collect and share the results of developing wheat varieties with durable and broad-spectrum resistance to major fungal, bacterial, and viral diseases through molecular breeding techniques.

The scope of this field encompasses the identification and functional analysis of resistance genes and quantitative trait loci (QTLs), the development and deployment of molecular markers, and the use of omics technologies (genomics, transcriptomics, and proteomics) to better understand host–pathogen interactions. It also includes the strategic pyramiding of multiple resistance genes for durable protection and the ongoing surveillance of pathogen evolution to ensure long-term effectiveness.

Dr. Monika Cseplo
Guest Editor

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Keywords

  • wheat disease resistance breeding
  • wheat pathogens
  • molecular markers
  • genomic selection and prediction
  • gene editing technologies
  • eco-friendly and sustainable farming systems
  • resistance genes
  • omics technologies
  • pathogen monitoring

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Published Papers (1 paper)

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Research

24 pages, 5813 KB  
Article
Small Non-Coding RNAs in the Regulatory Network of Wheat Dwarf Virus-Infected Wheat
by Abdoallah Sharaf, Jiban K. Kundu, Przemysław Nuc, Emad Ibrahim and Jan Ripl
Agriculture 2026, 16(1), 67; https://doi.org/10.3390/agriculture16010067 - 28 Dec 2025
Cited by 1 | Viewed by 735
Abstract
Wheat dwarf virus (WDV) is a major constraint to global wheat production, causing severe yield losses and economic disruption. Understanding the molecular basis of wheat–WDV interactions is essential for developing resistant cultivars. Non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), [...] Read more.
Wheat dwarf virus (WDV) is a major constraint to global wheat production, causing severe yield losses and economic disruption. Understanding the molecular basis of wheat–WDV interactions is essential for developing resistant cultivars. Non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), are key regulators of gene expression and defence. This study identified ncRNAs involved in wheat responses to WDV, including host lncRNAs, miRNAs, and viral small interfering RNAs (siRNAs) targeting WDV genomic regions. High-throughput sequencing revealed extensive ncRNA reprogramming under WDV infection. A total of 437 differentially expressed lncRNAs (DElncRNAs) and 58 miRNAs (DEmiRNAs) were detected. Resistant genotypes displayed more DElncRNAs (204 in Svitava; 163 in Fengyou 3) than the susceptible Akteur (141). In Akteur, 66.7% of DElncRNAs were downregulated, whereas in Svitava, 56.9% were upregulated. Akteur also exhibited more DEmiRNAs (28) than resistant genotypes (15), with predominant downregulation. A co-expression network analysis revealed 391 significant DElncRNA–mRNA interactions mediated by 16 miRNAs. The lncRNA XLOC_058282 was linked to 298 transcripts in resistant genotypes, suggesting a central role in the host defence. Functional annotation showed enrichment in signalling, metabolic, and defence-related pathways. Small RNA profiling identified 1166 differentially expressed sRNAs targeting WDV, including conserved hotspots and 408 genotype-specific sites in Akteur versus Fengyou 3. Infected plants displayed longer sRNAs, a sense-strand bias, and a 5′ uridine preference, but lacked typical 21–24 nt phasing. These findings highlight the central roles of ncRNAs in orchestrating wheat antiviral defence and provide a molecular framework for breeding virus-resistant wheat. Full article
(This article belongs to the Special Issue Molecular Breeding for Wheat Disease Resistance)
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