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Article

LC-QTOF/MS Untargeted Metabolomics of Sheep Milk under Cocoa Husks Enriched Diet

1
Dipartimento di Scienze della Vita e dell’Ambiente, Campus Universitario, 09042 Monserrato, Italy
2
Dipartimento di Agraria, Sezione di Scienze Zootecniche, Università degli Studi di Sassari, Viale Italia 39, 07100 Sassari, Italy
*
Author to whom correspondence should be addressed.
Dairy 2021, 2(1), 112-121; https://doi.org/10.3390/dairy2010011
Received: 15 December 2020 / Revised: 18 January 2021 / Accepted: 26 January 2021 / Published: 22 February 2021
(This article belongs to the Special Issue Innovation Meets Tradition in the Sheep and Goat Dairy Industry)
The aim of this work was to evaluate, by an untargeted metabolomics approach, changes of milk metabolites induced by the replacement of soybean hulls with cocoa husks in the ewes’ diet. Animals were fed with a soybean diet integrated with 50 or 100 g/d of cacao husks. Milk samples were analyzed by an ultra high performance liquid chromatograph coupled to a time of flight mass spectrometer (UHPLC-QTOF-MS) platform. Multivariate statistical analysis showed that the time of sampling profoundly affected metabolite levels, while differences between treatments were evident at the fourth week of sampling. Cocoa husks seem to induce level changes of milk metabolites implicated in the thyroid hormone metabolism and ubiquinol-10 biosynthesis. View Full-Text
Keywords: mass spectrometry; animals management; thyroid hormone metabolism; ubiquinol-10 biosynthesis mass spectrometry; animals management; thyroid hormone metabolism; ubiquinol-10 biosynthesis
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MDPI and ACS Style

Manis, C.; Scano, P.; Nudda, A.; Carta, S.; Pulina, G.; Caboni, P. LC-QTOF/MS Untargeted Metabolomics of Sheep Milk under Cocoa Husks Enriched Diet. Dairy 2021, 2, 112-121. https://doi.org/10.3390/dairy2010011

AMA Style

Manis C, Scano P, Nudda A, Carta S, Pulina G, Caboni P. LC-QTOF/MS Untargeted Metabolomics of Sheep Milk under Cocoa Husks Enriched Diet. Dairy. 2021; 2(1):112-121. https://doi.org/10.3390/dairy2010011

Chicago/Turabian Style

Manis, Cristina, Paola Scano, Anna Nudda, Silvia Carta, Giuseppe Pulina, and Pierluigi Caboni. 2021. "LC-QTOF/MS Untargeted Metabolomics of Sheep Milk under Cocoa Husks Enriched Diet" Dairy 2, no. 1: 112-121. https://doi.org/10.3390/dairy2010011

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