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Proceedings 2017, 1(10), 1000;

Investigation of Apoptotic Effect of Sinapic Acid in Hep3B and HepG2 Human Hepatocellular Carcinoma Cells

Department of Medical Biology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya 42060, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
Author to whom correspondence should be addressed.
Published: 13 November 2017
PDF [141 KB, uploaded 13 November 2017]


Hepatocellular carcinoma is one of the most common malignancies in worldwide. Sinapic acid (SA) is a phytochemical found in various fruits and vegetables. The aim of the study was to investigate the effect of SA on apoptosis in Hep3B and HepG2 hepatocellular carcinoma cells. XTT assay was used to determine the cytotoxic effect of SA. Total RNA isolation was conducted using TRIzol Reagent. Relative mRNA expression levels of important genes in apoptosis including CASP3, CASP7, CASP8, CASP9, BAX, BCL2, FAS and CYCS were quantified using qPCR in control and dose groups. The IC50 dose of SA in Hep3B and HepG2 cells were found to be 1000 µM and 1150 µM for 72 h, respectively. According to qPCR results, significant increases in the expressions of CASP3 and FAS were determined as 23.37 and 27.47 folds in treated Hep3B cells. On the other hand, SA in HepG2 cells caused a significant increase in the expressions of CASP3, CASP8, CASP9, BAX and FAS as 1.53, 1.77, 1.21, 1.47 and 1.39 folds respectively, compared with the control group. It is considered that SA may cause apoptosis in Hep3B and HepG2 cells. Further studies with SA should be conducted to can be therapeutic agent on hepatocellular carcinoma.
Keywords: apoptosis; Hep3B cells; HepG2 cells; sinapic acid apoptosis; Hep3B cells; HepG2 cells; sinapic acid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Eroğlu, C.; Kurar, E.; Avcı, E.; Vural, H. Investigation of Apoptotic Effect of Sinapic Acid in Hep3B and HepG2 Human Hepatocellular Carcinoma Cells. Proceedings 2017, 1, 1000.

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