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J. Imaging, Volume 4, Issue 1 (January 2018)
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Cover Story (view full-size image) This paper presents the first results of bacterial and cellular load estimation in the human distal [...] Read more. This paper presents the first results of bacterial and cellular load estimation in the human distal lung where pathologies such as pneumonia occur. In pulmonary fibre confocal fluorescence microscopy (FCFM) lung tissue is autofluorescent, while bacteria appear as fluorescent dots when exposed to a targeted substance. Bacterial dots are visible in panel d (post-substance) but not in panel b (pre-substance). We learn spatio-temporal templates of a bacterium and cell from the FCFM videos, and use them as centers of a radial basis function network to count the number of cells and bacteria. View this paper