Desferrioxamine B-Mediated Pre-Clinical In Vivo Imaging of Infection by the Mold Fungus Aspergillus fumigatus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Experiments
2.2. Ex Vivo Biodistribution
2.3. Animal Imaging
2.4. Synthesis of the Derivatives
2.4.1. Analytical RP-HPLC
2.4.2. Preparative RP-HPLC
2.4.3. Fe-DFO-B
2.4.4. Fe-DFO-Bac and Fe-DFOsuc
2.4.5. Analytical Data
2.5. Radiolabelling
2.6. Short-Term Siderophore Uptake Analysis
2.7. Analysis of Siderophore Utilization Via Growth Promotion
3. Results
3.1. [68Ga]Ga-DFO-B Allows In Vivo PET Imaging of Pulmonary Aspergillosis in a Rat Model
3.2. In Vivo Uptake of [67/68Ga]Ga-DFO-B Is Comparable to [67/68Ga]Ga-DFO-E
3.3. Chemical Modification of DFO-B Improves Its Growth Promotion Activity at Acidic pH
3.4. Short-Term Uptake of DFO-B Displays pH Dependence
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Misslinger, M.; Petrik, M.; Pfister, J.; Hubmann, I.; Bendova, K.; Decristoforo, C.; Haas, H. Desferrioxamine B-Mediated Pre-Clinical In Vivo Imaging of Infection by the Mold Fungus Aspergillus fumigatus. J. Fungi 2021, 7, 734. https://doi.org/10.3390/jof7090734
Misslinger M, Petrik M, Pfister J, Hubmann I, Bendova K, Decristoforo C, Haas H. Desferrioxamine B-Mediated Pre-Clinical In Vivo Imaging of Infection by the Mold Fungus Aspergillus fumigatus. Journal of Fungi. 2021; 7(9):734. https://doi.org/10.3390/jof7090734
Chicago/Turabian StyleMisslinger, Matthias, Milos Petrik, Joachim Pfister, Isabella Hubmann, Katerina Bendova, Clemens Decristoforo, and Hubertus Haas. 2021. "Desferrioxamine B-Mediated Pre-Clinical In Vivo Imaging of Infection by the Mold Fungus Aspergillus fumigatus" Journal of Fungi 7, no. 9: 734. https://doi.org/10.3390/jof7090734