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Resistance to the SDHI Fungicides Boscalid and Fluopyram in Podosphaera xanthii Populations from Commercial Cucurbit Fields in Spain
 
 
Article

Gene Mining for Conserved, Non-Annotated Proteins of Podosphaera xanthii Identifies Novel Target Candidates for Controlling Powdery Mildews by Spray-Induced Gene Silencing

1
Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain
2
Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”-Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29071 Málaga, Spain
*
Author to whom correspondence should be addressed.
Academic Editors: Antonieta De Cal, Inmaculada Larena and Paloma Melgarejo
J. Fungi 2021, 7(9), 735; https://doi.org/10.3390/jof7090735
Received: 14 August 2021 / Revised: 31 August 2021 / Accepted: 6 September 2021 / Published: 8 September 2021
(This article belongs to the Special Issue Control of Fungal Diseases in Crops)
The powdery mildew fungus Podosphaera xanthii is one of the most important limiting factors for cucurbit production worldwide. Despite the significant efforts made by breeding and chemical companies, effective control of this pathogen remains elusive to growers. In this work, we examined the suitability of RNAi technology called spray-induced gene silencing (SIGS) for controlling cucurbit powdery mildew. Using leaf disc and cotyledon infiltration assays, we tested the efficacy of dsRNA applications to induce gene silencing in P. xanthii. Furthermore, to identify new target candidate genes, we analyzed sixty conserved and non-annotated proteins (CNAPs) deduced from the P. xanthii transcriptome in silico. Six proteins presumably involved in essential functions, specifically respiration (CNAP8878, CNAP9066, CNAP10905 and CNAP30520), glycosylation (CNAP1048) and efflux transport (CNAP948), were identified. Functional analysis of these CNAP coding genes by dsRNA-induced gene silencing resulted in strong silencing phenotypes with large reductions in fungal growth and disease symptoms. Due to their important contributions to fungal development, the CNAP1048, CNAP10905 and CNAP30520 genes were selected as targets to conduct SIGS assays under plant growth chamber conditions. The spray application of these dsRNAs induced high levels of disease control, supporting that SIGS could be a sustainable approach to combat powdery mildew diseases. View Full-Text
Keywords: cucurbits; dsRNA; Podosphaera xanthii; powdery mildews; RNAi; spray-induced gene silencing (SIGS) cucurbits; dsRNA; Podosphaera xanthii; powdery mildews; RNAi; spray-induced gene silencing (SIGS)
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MDPI and ACS Style

Ruiz-Jiménez, L.; Polonio, Á.; Vielba-Fernández, A.; Pérez-García, A.; Fernández-Ortuño, D. Gene Mining for Conserved, Non-Annotated Proteins of Podosphaera xanthii Identifies Novel Target Candidates for Controlling Powdery Mildews by Spray-Induced Gene Silencing. J. Fungi 2021, 7, 735. https://doi.org/10.3390/jof7090735

AMA Style

Ruiz-Jiménez L, Polonio Á, Vielba-Fernández A, Pérez-García A, Fernández-Ortuño D. Gene Mining for Conserved, Non-Annotated Proteins of Podosphaera xanthii Identifies Novel Target Candidates for Controlling Powdery Mildews by Spray-Induced Gene Silencing. Journal of Fungi. 2021; 7(9):735. https://doi.org/10.3390/jof7090735

Chicago/Turabian Style

Ruiz-Jiménez, Laura, Álvaro Polonio, Alejandra Vielba-Fernández, Alejandro Pérez-García, and Dolores Fernández-Ortuño. 2021. "Gene Mining for Conserved, Non-Annotated Proteins of Podosphaera xanthii Identifies Novel Target Candidates for Controlling Powdery Mildews by Spray-Induced Gene Silencing" Journal of Fungi 7, no. 9: 735. https://doi.org/10.3390/jof7090735

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