- Article
Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection of Mycoplasma bovis: Comparison of Assay Performance with a Conventional PCR Assay and Another Taqman Real-Time PCR Assay
- Hemant Naikare,
- Daniela Bruno,
- Debabrata Mahapatra,
- Alesia Reinisch,
- Russell Raleigh and
- Robert Sprowls
The objective of this study was to develop and validate a Taqman real-time PCR assay for the detection of Mycoplasma bovis (M. bovis). Unique primers targeting the highly conserved house-keeping gene (uvrC) were designed and the probe sequence was de...

