Next Article in Journal
Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection of Mycoplasma bovis: Comparison of Assay Performance with a Conventional PCR Assay and Another Taqman Real-Time PCR Assay
Previous Article in Journal
A Physician’s View of One Health: Challenges and Opportunities
Open AccessCommunication

Optimizing the Measurement of Colostrum Antibody Concentrations for Identifying BVDV Persistently Infected Calves

School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Duncan C. Ferguson and Margarethe Hoenig
Vet. Sci. 2015, 2(1), 26-31; https://doi.org/10.3390/vetsci2010026
Received: 27 January 2015 / Revised: 27 February 2015 / Accepted: 5 March 2015 / Published: 9 March 2015
Colostrum contains substantially higher concentrations of immunoglobulins compared to serum, which may help to improve the utility of diagnostic tests. The aim of this study was to determine the diagnostic value of colostrum antibody concentrations in identifying Bovine Viral Diarrhoea Virus (BVDV) PI (persistently infected) calf carrying beef heifers following an experimental infection. Colostrum was collected within 12 hours of parturition and tested in undiluted, 1:5, 1:10, 1:100, 1:200, and 1:500 dilutions using an enzyme-linked immunosorbent assay (ELISA) for BVDV antibody. Cows were determined to be carrying a PI calf based on positive quantitative Real Time-Polymerase Chain Reaction and antigen ELISA result on pre-colostral serum and ear notch samples collected from their calf. The median ELISA sample-to-positive (S/P) ratio for colostrum collected from heifers that carried a PI calf were significantly higher than the median ELISA S/P ratio for colostrum collected from heifers that did not carry a PI calf at dilutions of 1:100, 1:200, and 1:500. This study provides further evidence for increased antigenic stimulation in utero by the BVDV viraemic PI calf, which can also be identified with 100% diagnostic sensitivity when using 1:500 dilution colostrum. View Full-Text
Keywords: BVDV; colostrum; ELISA; PI calves BVDV; colostrum; ELISA; PI calves
Show Figures

Figure 1

MDPI and ACS Style

Jenvey, C.J.; Reichel, M.P.; Lanyon, S.R.; Cockcroft, P.D. Optimizing the Measurement of Colostrum Antibody Concentrations for Identifying BVDV Persistently Infected Calves. Vet. Sci. 2015, 2, 26-31.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop