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Bioengineering, Volume 12, Issue 6

June 2025 - 119 articles

Cover Story: A novel protein engineering approach enhances the crystallization of the leaf branch compost cutinase (LCC) quadruple mutant ICCG, a key enzyme for effective PET recycling under mild conditions. By introducing targeted electrostatic interactions—specifically Arg–Glu pairs—at crystal contact sites, researchers generated the ICCGY T110E mutant with improved crystallizability in terms of faster crystallization at decreased protein concentrations. Controlled mutations at non-interacting sites confirmed the specificity of this strategy, with preservation of activity. This advancement facilitates more efficient downstream processing of biocatalysts, offering a rational method to streamline enzyme purification and support sustainable industrial biotechnology applications. View this paper
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Articles (119)

  • Article
  • Open Access
1 Citations
2,776 Views
16 Pages

AI-Powered Spectral Imaging for Virtual Pathology Staining

  • Adam Soker,
  • Maya Almagor,
  • Sabine Mai and
  • Yuval Garini

Pathological analysis of tissue biopsies remains the gold standard for diagnosing cancer and other diseases. However, this is a time-intensive process that demands extensive training and expertise. Despite its importance, it is often subjective and n...

  • Article
  • Open Access
1 Citations
1,206 Views
21 Pages

Real-time information on key state variables during fermentation is crucial for the effective optimization and control of bioprocesses. Specialized sensors for online or at-line monitoring of these variables are often associated with high costs, espe...

  • Article
  • Open Access
3,198 Views
15 Pages

Artificial Intelligence Outperforms Physicians in General Medical Knowledge, Except in the Paediatrics Domain: A Cross-Sectional Study

  • Joana Miranda,
  • Raquel Pereira-Silva,
  • João Guichard,
  • Jorge Meneses,
  • Andreia Neves Carreira and
  • Daniela Seixas

Generative artificial intelligence (genAI) shows promising results in clinical practice. This study compared a GPT-4-turbo virtual assistant with physicians from Italy, France, Spain, and Portugal on medical knowledge derived from national exams whil...

  • Review
  • Open Access
1 Citations
1,950 Views
16 Pages

This review addresses the exposure risks associated with the inhalation of aerosolized cosmetic products and explores the utility of lung organoids in assessing these risks. Aerosolized cosmetics such as sprays pose potential health hazards through i...

  • Review
  • Open Access
1 Citations
1,653 Views
23 Pages

OCT in Oncology and Precision Medicine: From Nanoparticles to Advanced Technologies and AI

  • Sanam Daneshpour Moghadam,
  • Bogdan Maris,
  • Ali Mokhtari,
  • Claudia Daffara and
  • Paolo Fiorini

Optical Coherence Tomography (OCT) is a relatively new medical imaging device that provides high-resolution and real-time visualization of biological tissues. Initially designed for ophthalmology, OCT is now being applied in other types of pathologie...

  • Article
  • Open Access
8 Citations
2,592 Views
39 Pages

Hierarchical Swin Transformer Ensemble with Explainable AI for Robust and Decentralized Breast Cancer Diagnosis

  • Md. Redwan Ahmed,
  • Hamdadur Rahman,
  • Zishad Hossain Limon,
  • Md Ismail Hossain Siddiqui,
  • Mahbub Alam Khan,
  • Al Shahriar Uddin Khondakar Pranta,
  • Rezaul Haque,
  • S M Masfequier Rahman Swapno,
  • Young-Im Cho and
  • Mohamed S. Abdallah

Early and accurate detection of breast cancer is essential for reducing mortality rates and improving clinical outcomes. However, deep learning (DL) models used in healthcare face significant challenges, including concerns about data privacy, domain-...

  • Review
  • Open Access
2 Citations
1,112 Views
26 Pages

Toward Integrative Biomechanical Models of Osteochondral Tissues: A Multilayered Perspective

  • Bruna Silva,
  • Marco Domingos,
  • Sandra Amado,
  • Juliana R. Dias,
  • Paula Pascoal-Faria,
  • Ana C. Maurício and
  • Nuno Alves

Understanding the complex mechanical behavior of osteochondral tissues in silico is essential for improving experimental models and advancing research in joint health and degeneration. This review provides a comprehensive analysis of the constitutive...

  • Article
  • Open Access
1,173 Views
12 Pages

Spinal cord injury (SCI) often necessitates the use of a manual wheelchair, which can overload the shoulders and contribute to upper extremity (UE) pain. Currently, no standardized methods exist to assess UE kinematics during wheelchair propulsion. T...

  • Review
  • Open Access
1,239 Views
13 Pages

Background: Steal syndrome in the setting of microvascular reconstruction refers to a phenomenon whereby blood flow is diverted from the native tissue to the free flap, leading to ischemia and potential limb loss. In the present study, we aim to comp...

  • Article
  • Open Access
1,144 Views
14 Pages

Exploration of Cytokines That Impact the Therapeutic Efficacy of Mesenchymal Stem Cells in Alzheimer’s Disease

  • Herui Wang,
  • Chonglin Zhong,
  • Yi Mi,
  • Guo Li,
  • Chenliang Zhang,
  • Yaoyao Chen,
  • Xin Li,
  • Yongjun Liu and
  • Guangyang Liu

Current therapies for Alzheimer’s disease (AD) includes acetylcholinesterase inhibitors, NMDA receptor antagonists, and amyloid beta (Aβ)/Tau-targeting drugs. While these drugs improve cognitive decline and target the pathological mechanis...

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Bioengineering - ISSN 2306-5354