Toxics

24 pages, 5241 KiB

Open AccessReview

Global Environmental Geochemistry and Molecular Speciation of Heavy Metals in Soils and Groundwater from Abandoned Smelting Sites: Analysis of the Contamination Dynamics and Remediation Alternatives in Karst Settings

by Hang Xu, Qiao Han, Muhammad Adnan, Mengfei Li, Mingshi Wang, Mingya Wang, Fengcheng Jiang and Xixi Feng

Toxics 2025, 13(7), 608; https://doi.org/10.3390/toxics13070608 - 21 Jul 2025

Viewed by 238

Abstract

Abandoned smelting sites in karst terrain pose a serious environmental problem due to the complex relationship between specific hydrogeological elements and heavy metal contamination. This review combines work from across the globe to consider how karst-specific features (i.e., rapid underground drainage, high permeability, [...] Read more.

Abandoned smelting sites in karst terrain pose a serious environmental problem due to the complex relationship between specific hydrogeological elements and heavy metal contamination. This review combines work from across the globe to consider how karst-specific features (i.e., rapid underground drainage, high permeability, and carbonate mineralogy) influence the mobility, speciation, and bioavailability of “metallic” pollutants, such as Pb, Cd, Zn, and As. In some areas, such as Guizhou (China), the Cd content in the surface soil is as high as 23.36 mg/kg, indicating a regional risk. Molecular-scale analysis, such as synchrotron-based XAS, can elucidate the speciation forms that underlie toxicity and remediation potential. Additionally, we emphasize discrepancies between karst in Asia, Europe, and North America and synthesize cross-regional contamination events. The risk evaluation is complicated, particularly when dynamic flow systems and spatial heterogeneity are permanent, and deep models like DI-NCPI are required as a matter of course. The remediation is still dependent on the site; however, some technologies, such as phytoremediation, biosorption, and bioremediation, are promising if suitable geochemical and microbial conditions are present. This review presents a framework for integrating molecular data and hydrogeological concepts to inform the management of risk and sustainable remediation of legacy metal pollution in karst. Full article

(This article belongs to the Section Toxicity Reduction and Environmental Remediation)

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20 pages, 1258 KiB

Open AccessArticle

The Crime of Vehicular Homicide in Italy: Trends in Alcohol and Drug Use in Fatal Road Accidents in Lazio Region from 2018 to 2024

by Francesca Vernich, Leonardo Romani, Federico Mineo, Giulio Mannocchi, Lucrezia Stefani, Margherita Pallocci, Luigi Tonino Marsella, Michele Treglia and Roberta Tittarelli

Toxics 2025, 13(7), 607; https://doi.org/10.3390/toxics13070607 - 19 Jul 2025

Viewed by 135

Abstract

In Italy, the law on road homicide (Law no. 41/2016) introduced specific provisions for drivers who cause severe injuries or death to a person due to the violation of the Highway Code. The use of alcohol or drugs while driving constitutes an aggravating [...] Read more.

In Italy, the law on road homicide (Law no. 41/2016) introduced specific provisions for drivers who cause severe injuries or death to a person due to the violation of the Highway Code. The use of alcohol or drugs while driving constitutes an aggravating circumstance of the offence and provides for a tightening of penalties. Our study aims to report on the analysis performed on blood samples collected between January 2018 and December 2024 from drivers convicted of road homicide and who tested positive for alcohol and/or drugs. The majority of the involved subjects were males belonging to the 18–30 and 41–50 age groups. Alcohol, cocaine and cannabinoids were the most detected substances and the most frequent polydrug combination was alcohol and cocaine. We also investigated other influencing factors in road traffic accidents as the day of the week and the time of the day in which fatal road traffic accident occurred, and the time elapsed between the road accident and the collection of biological samples. Our data, in line with the international scenario, strongly support that, in addition to the tightening of penalties, raising awareness plays a key role in preventing alcohol- and drug-related traffic accidents by increasing risk perception and encouraging safer driving behaviors. Full article

(This article belongs to the Special Issue Current Issues and Research Perspectives in Forensic Toxicology)

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24 pages, 1399 KiB

Open AccessSystematic Review

Nephrotoxicity of New Antibiotics: A Systematic Review

by Panagiotis Stathopoulos, Laura T. Romanos, Charalampos Loutradis and Matthew E. Falagas

Toxics 2025, 13(7), 606; https://doi.org/10.3390/toxics13070606 - 19 Jul 2025

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Abstract

Drug-induced nephrotoxicity is a common and serious problem in clinical practice. We conducted a systematic review of studies reporting nephrotoxicity events associated with antibiotics approved since 2018. The agents assessed included aztreonam/avibactam, cefepime/enmetazobactam, cefiderocol, ceftobiprole, contezolid, gepotidacin, imipenem/cilastatin/relebactam, lascufloxacin, lefamulin, levonadifloxacin, plazomicin, and [...] Read more.

Drug-induced nephrotoxicity is a common and serious problem in clinical practice. We conducted a systematic review of studies reporting nephrotoxicity events associated with antibiotics approved since 2018. The agents assessed included aztreonam/avibactam, cefepime/enmetazobactam, cefiderocol, ceftobiprole, contezolid, gepotidacin, imipenem/cilastatin/relebactam, lascufloxacin, lefamulin, levonadifloxacin, plazomicin, and sulbactam/durlobactam. Literature searches were conducted in PubMed, Scopus, Web of Science, and major pharmacovigilance databases (Vigibase, FAERS, EudraVigilance, EMA, FDA, NMPA, PMDA, and CDSCO) in May 2025, along with reference citation tracking. Studies were included if they reported safety or adverse event data. The risk of bias was assessed using validated tools in accordance with the study design. Out of 2105 potentially relevant records, 74 studies met inclusion criteria, comprising 52 clinical trials, 17 observational studies, 1 registry-based study, 3 case series, and 1 case report. Nephrotoxicity was rarely reported for any of the newly approved antibiotics. No renal adverse events were found in the available studies for aztreonam/avibactam, levonadifloxacin, and contezolid. Most studies were of moderate to high quality; two were classified as low quality. However, nephrotoxicity was inconsistently assessed, with variable definitions and methodologies used. Although current data suggest a low frequency of nephrotoxicity, limitations in study design and reporting preclude firm conclusions. There is a need for post-marketing studies to better characterize renal safety. Clinicians should remain vigilant and continue to monitor for and report renal-related adverse events. Full article

(This article belongs to the Special Issue Nephrotoxicity Induced by Drugs and Chemicals in the Environment)

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19 pages, 1944 KiB

Open AccessArticle

Impact of Polystyrene Microplastics on Human Sperm Functionality: An In Vitro Study of Cytotoxicity, Genotoxicity and Fertility-Related Genes Expression

by Filomena Mottola, Maria Carannante, Ilaria Palmieri, Lorenzo Ibello, Luigi Montano, Mariaceleste Pezzullo, Nicola Mosca, Nicoletta Potenza and Lucia Rocco

Toxics 2025, 13(7), 605; https://doi.org/10.3390/toxics13070605 - 19 Jul 2025

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Abstract

Polystyrene microplastics (PS-MPs) released in the environment reportedly affect the reproduction of various organisms, induced oxidative stress and apoptosis, resulting in altered sperm parameters. In this in vitro study, we tested the cytotoxicity and genotoxicity of PS-MPs by exposing human semen samples to [...] Read more.

Polystyrene microplastics (PS-MPs) released in the environment reportedly affect the reproduction of various organisms, induced oxidative stress and apoptosis, resulting in altered sperm parameters. In this in vitro study, we tested the cytotoxicity and genotoxicity of PS-MPs by exposing human semen samples to PS-MPs levels (105 and 210 μg/mL) for 30–60–90 min. Semen parameters, genome stability, sperm DNA fragmentation (SDF) and reactive oxygen species (ROS) production were analyzed before and after exposure. Moreover, we also evaluated the expression level of spermatozoa-specific expressed genes essential for the fusion with oocyte (DCST1, DCST2, IZUMO1, SPACA6, SOF1, and TMEM95). After PS-MP exposure, semen concentration and morphology did not differ, while sperm vitality and motility decreased in a time-dependent manner. In addition, sperm agglutination was observed in the groups exposed to both PS-MPs concentrations tested. A time- and concentration-dependent reduction in genomic stability, as well as increased SDF and ROS production, was also observed. Moreover, all investigated transcripts were down-regulated after PS-MP exposure. Our results confirm the oxidative stress-mediated genotoxicity and cytotoxicity of PS-MPs on human spermatozoa. The sperm agglutination observed after treatment could be due to the aggregation of PS-MPs already adhered to the sperm membranes, hindering sperm movement and fertilizing capability. Interestingly, the downregulation of genes required for sperm–oocyte fusion, resulting from data on the in vitro experimental system, suggests that PS-MP exposure may have implications for sperm functionality. While these findings highlight potential mechanisms of sperm dysfunction, further investigations using in vivo models are needed to determine their broader biological implications. Possible environmental and working exposure to pollutants should be considered during the counselling for male infertility. Full article

(This article belongs to the Section Reproductive and Developmental Toxicity)

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23 pages, 4894 KiB

Open AccessArticle

Evaluating Copper-Induced Oxidative Stress in Germinating Wheat Seeds Using Laser Photoacoustic Spectroscopy and EPR Techniques

by Mioara Petrus, Cristina Popa, Ana-Maria Bratu, Alexandra Camelia Joita and Vasile Bercu

Toxics 2025, 13(7), 604; https://doi.org/10.3390/toxics13070604 - 18 Jul 2025

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Abstract

Copper is an essential micronutrient for plants, but excessive levels can induce toxicity and impair physiological functions. This study evaluates the toxic effects of copper sulfate (CuSO₄) on the germination of common wheat (Triticum aestivum), with emphasis on the [...] Read more.

Copper is an essential micronutrient for plants, but excessive levels can induce toxicity and impair physiological functions. This study evaluates the toxic effects of copper sulfate (CuSO₄) on the germination of common wheat (Triticum aestivum), with emphasis on the gas emission dynamics and oxidative stress biomarkers. Seeds were germinated in agar and exposed to CuSO₄ at concentrations of 1 µM, 100 µM, 1 mM, and 10 mM; distilled water served as the control. Ethylene and ammonia emissions were quantified using CO₂ laser photoacoustic spectroscopy, while electron paramagnetic resonance (EPR) spectroscopy was employed to detect free radicals and Cu²⁺ complexes. Exposure to Cu concentrations ≥ 1 mM significantly inhibited germination and biomass accumulation. Enhanced ethylene and ammonia emissions, particularly at 10 mM, indicated stress-related metabolic responses. The EPR spectra confirmed the presence of semiquinone radicals and Cu²⁺ complexes under higher Cu levels. These results demonstrate that photoacoustic and EPR techniques are effective tools for the early detection of metal-induced phytotoxicity and offer a non-invasive approach to environmental toxicity screening and plant stress assessment. Full article

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13 pages, 793 KiB

Open AccessArticle

Environmental Risk and Management of Iron Tailings in Road Subgrade

by Xiaowei Xu, Dapeng Zhang, Jie Cao, Chaoyue Wu, Yi Wang, Jing Hua, Zehua Zhao, Jun Zhang and Qi Yu

Toxics 2025, 13(7), 603; https://doi.org/10.3390/toxics13070603 - 17 Jul 2025

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Abstract

The utilization of iron tailings in road construction poses significant environmental risks due to the complex release mechanisms of pollutants and varying regional conditions. This study integrates an exponential decay model with an instantaneous pollutant transport model, employing Monte Carlo simulations to assess [...] Read more.

The utilization of iron tailings in road construction poses significant environmental risks due to the complex release mechanisms of pollutants and varying regional conditions. This study integrates an exponential decay model with an instantaneous pollutant transport model, employing Monte Carlo simulations to assess risks and regional characteristics. Results show high Potential Hazard Indices (PHIs) for arsenic, manganese, barium, nickel, and lead, with PHI values between 4.2 and 22.7. Simulations indicate that manganese and nickel concentrations may exceed groundwater standards, particularly in humid areas. The study recommends controlling the iron tailings mixing ratio based on climate, suggesting limits of 35% in humid, 60% in semi-humid, and more lenient ratios in arid and semi-arid regions. It also underscores the need for improved risk assessment methodologies and region-specific management strategies at the national level. Full article

(This article belongs to the Special Issue Soil Heavy Metal Pollution and Human Health)

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16 pages, 747 KiB

Open AccessArticle

Thermoset Polyester Resin Microplastics: Effects on Enzymatic Biomarkers and Toxicological Endpoint Responses of Eisenia fetida Earthworms

by David Amaya-Vías, Gemma Albendín, Vanessa Aranda-Quirós, Rocío Rodríguez-Barroso, Dolores Coello and Juana María Arellano

Toxics 2025, 13(7), 602; https://doi.org/10.3390/toxics13070602 - 17 Jul 2025

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Abstract

Thermosets are plastic composite materials widely used in many industrial sectors of modern society with an increasing presence in the environment. The adverse effects of this material on environmental compartments and biota of thermosets are still unknown. In this work, we studied the [...] Read more.

Thermosets are plastic composite materials widely used in many industrial sectors of modern society with an increasing presence in the environment. The adverse effects of this material on environmental compartments and biota of thermosets are still unknown. In this work, we studied the potential effects of two thermoset polyester resin-derived microplastics (R930A-SP and R930A-DVE1) on the survival, behavior, morphological changes and subcellular damage of earthworms Eisenia fetida. The proposed experimental conditions simulated environmentally relevant concentrations, taking as a reference other related microplastics present in the environment. Thus, E. fetida specimens were exposed to five concentrations (100, 500, 1000, 1500 and 2000 mg resin per kg soil) of these two resins for 14 days. At concentrations and exposure times studied, no significant effects on growth, measured as weight loss, or on the enzyme biomarkers (cholinesterase, carboxylesterase and glutathione S-transferase) were observed. Similarly, no behavioral changes were detected in earthworms, and the survival rate was 100%. Likewise, no differences were observed between the different formulations of the polyester resins studied. However, this study could serve as a starting point for further studies with higher concentrations and/or exposure times, as well as in combination with other pollutants. Full article

(This article belongs to the Special Issue Ecotoxicological Effects of Microplastics on the Soil Environment)

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32 pages, 3188 KiB

Open AccessArticle

Forty Years After Chernobyl: Radiocaesium in Wild Edible Mushrooms from North-Eastern Poland and Its Relevance for Dietary Exposure and Food Safety

by Iwona Mirończuk-Chodakowska, Jacek Kapała, Karolina Kujawowicz, Monika Sejbuk and Anna Maria Witkowska

Toxics 2025, 13(7), 601; https://doi.org/10.3390/toxics13070601 - 17 Jul 2025

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Abstract

Wild-growing edible mushrooms are known to bioaccumulate radionuclides from their environment, particularly the natural isotope potassium-40 (⁴⁰K) and anthropogenic cesium-137 (¹³⁷Cs). However, region-specific data for commercially relevant species in north-eastern Poland remain limited, despite the cultural and economic importance [...] Read more.

Wild-growing edible mushrooms are known to bioaccumulate radionuclides from their environment, particularly the natural isotope potassium-40 (⁴⁰K) and anthropogenic cesium-137 (¹³⁷Cs). However, region-specific data for commercially relevant species in north-eastern Poland remain limited, despite the cultural and economic importance of mushroom foraging and export. This study aimed to assess the radiological safety of wild mushrooms intended for human consumption, with particular attention to regulatory compliance and potential exposure levels. In this study, 230 mushroom samples representing 19 wild edible species were analyzed using gamma spectrometry, alongside composite soil samples collected from corresponding foraging sites. The activity concentration of ¹³⁷Cs in mushrooms ranged from 0.94 to 159.0 Bq/kg fresh mass (f.m.), and that of ⁴⁰K from 64.4 to 150.2 Bq/kg f.m. None of the samples exceeded the regulatory limit of 1250 Bq/kg f.m. for ¹³⁷Cs. The highest estimated annual effective dose was 2.32 µSv from ¹³⁷Cs and 0.93 µSv from ⁴⁰K, with no exceedance of regulatory limits observed in any sample. A strong positive correlation was observed between ¹³⁷Cs activity in soil and mushroom dry mass (Spearman’s Rho = 0.81, p = 0.042), supporting predictable transfer patterns. Additionally, the implications of mushroom drying were assessed considering Council Regulation (Euratom) 2016/52, which mandates radionuclide levels in dried products be evaluated based on their reconstituted form. After such adjustment, even the most contaminated dried samples were found to comply with food safety limits. These findings confirm the radiological safety of wild mushrooms from north-eastern Poland and contribute novel data for a region with limited prior monitoring, in the context of current food safety regulations. Full article

(This article belongs to the Section Agrochemicals and Food Toxicology)

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17 pages, 449 KiB

Open AccessArticle

Immunotoxicity Studies on the Insecticide 2-((1-(4-Phenoxyphenoxy)propan-2-yl)oxy)pyridine (MPEP) in Hsd:Harlan Sprague Dawley SD^® Rats

by Victor J. Johnson, Stefanie C. M. Burleson, Michael I. Luster, Gary R. Burleson, Barry McIntyre, Veronica G. Robinson, Reshan A. Fernando, James Blake, Donna Browning, Stephen Cooper, Shawn Harris and Dori R. Germolec

Toxics 2025, 13(7), 600; https://doi.org/10.3390/toxics13070600 - 17 Jul 2025

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Abstract

The broad-spectrum insect growth regulator (IGR) and insecticide 2-((1-(4-Phenoxyphenoxy)propan-2-yl)oxy)pyridine (MPEP; also known as pyriproxyfen) is increasingly being used to address public health programs for vector control, initiated by the spread of Zika virus in 2015–2016. While considered relatively safe for humans under normal [...] Read more.

The broad-spectrum insect growth regulator (IGR) and insecticide 2-((1-(4-Phenoxyphenoxy)propan-2-yl)oxy)pyridine (MPEP; also known as pyriproxyfen) is increasingly being used to address public health programs for vector control, initiated by the spread of Zika virus in 2015–2016. While considered relatively safe for humans under normal conditions, limited toxicology data are available. Current studies were undertaken to address the data gap regarding potential immunotoxicity of MPEP, with particular emphasis on host resistance to viral infection. Hsd:Harlan Sprague Dawley SD^® rats were treated for 28 days by oral gavage with doses of 0, 62.5, 125, 250 or 500 mg/kg/day of MPEP in corn oil. There was a dose-dependent increase in liver weights which is consistent with the liver playing a dominant role in MPEP metabolism. However, no histological correlates were observed. Following treatment, rats were subjected to a battery of immune tests as well as an established rat model of influenza virus infection to provide a comprehensive assessment of immune function and host resistance. While several of the immune tests showed minor exposure-related changes, evidenced by negative dose–response trends, most did not show significant differences in any of the MPEP treatment groups relative to vehicle control. Most notable was a negative trend in pulmonary mononuclear cell phagocytosis with increases in dose of MPEP. There was also a positive trend in early humoral immune response (5 days after immunization) to keyhole limpet hemocyanin (KLH) as evidenced by increased serum anti-KLH IgM antibodies which was followed later (14 days following immunization) by decreasing trends in anti-KLH IgM and IgG antibody levels. However, MPEP treatment had no effect on the ability of rats to clear the influenza virus nor the T-dependent IgM and IgG antibody response to the virus. The lack of effects of MPEP on host resistance to influenza suggests the immune effects were minimal and unlikely to present a hazard with respect to susceptibility to respiratory viral infection. Full article

(This article belongs to the Special Issue Environmental Contaminants and Human Health—2nd Edition)

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23 pages, 5171 KiB

Open AccessArticle

Investigation into the Enhancement Effects of Combined Bioremediation of Petroleum-Contaminated Soil Utilizing Immobilized Microbial Consortium and Sudan Grass

by Tie-Jun Wang, Zi-Yue Ding, Zi-Wei Hua, Zi-Wang Yuan, Qiu-Hong Niu and Hao Zhang

Toxics 2025, 13(7), 599; https://doi.org/10.3390/toxics13070599 - 16 Jul 2025

Viewed by 209

Abstract

Petroleum-contaminated soil is an increasingly severe environmental issue. The integration of phytoremediation and microbial remediation can effectively mitigate their individual limitations and enhance remediation efficiency. In this study, four newly isolated bacterial strains (including Cytobacillus and Rhodococcus) that exhibited preferential degradation of [...] Read more.

Petroleum-contaminated soil is an increasingly severe environmental issue. The integration of phytoremediation and microbial remediation can effectively mitigate their individual limitations and enhance remediation efficiency. In this study, four newly isolated bacterial strains (including Cytobacillus and Rhodococcus) that exhibited preferential degradation of distinct petroleum components were combined with the rhamnolipid-producing strain Pseudomonas aeruginosa SL-1. The immobilization of this petroleum-degrading microbial consortium was performed by biochar adsorption and sodium alginate embedding, subsequently optimized using response surface methodology (0.75 g·L⁻¹ of biochar, 40 g·L⁻¹ of sodium alginate, and 40 g·L⁻¹ of calcium chloride). The results showed that the highest petroleum degradation rate (97.1%) of immobilized bacterial consortium was achieved at 72 h at a petroleum concentration of 5.0 g·L⁻¹. When combined with Sudan grass for soil bioremediation, the degradation rate reached 72.8% after 120 d for soil containing 5.0 g·kg⁻¹ of petroleum, higher than the results for the treatments with only immobilized bacterial consortium (53.0%) or Sudan grass (49.2%). Furthermore, significant improvements were observed for soil pH; nitrogen, phosphorus, and potassium contents; and urease, dehydrogenase, and catalase activities. Composite treatment also significantly increased the diversity and richness of the soil bacterial community and regulated its structure, function, and network composition. This study offers theoretical insights and potential practical applications for the enhanced bioremediation of petroleum-contaminated soils. Full article

(This article belongs to the Special Issue Multi-Media Environmental Pollution and Health Risks: Tracing, Effects, and Collaborative Governance)

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13 pages, 1476 KiB

Open AccessArticle

Interactive Effects of Ambient Ozone and Meteorological Factors on Cerebral Infarction: A Five-Year Time-Series Study

by Yanzhe Chen, Songtai Yang, Hanya Que, Jiamin Liu, Zhe Wang, Na Wang, Yunkun Qin, Meng Li and Fang Zhou

Toxics 2025, 13(7), 598; https://doi.org/10.3390/toxics13070598 - 16 Jul 2025

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Abstract

Objective: Our objective was to investigate the short-term effects of ambient ozone (O₃) meteorological factors and their interactions on hospitalizations for cerebral infarction in Zhengzhou, China. Methods: Daily data on air pollutants, meteorological factors, and hospitalization of cerebral infarction patients [...] Read more.

Objective: Our objective was to investigate the short-term effects of ambient ozone (O₃) meteorological factors and their interactions on hospitalizations for cerebral infarction in Zhengzhou, China. Methods: Daily data on air pollutants, meteorological factors, and hospitalization of cerebral infarction patients were collected from 1 January 2019 to 31 December 2023 in Zhengzhou, China. A generalized additive model was constructed to evaluate the association between ambient O₃ levels and hospitalization for cerebral infarction. A distributed lag non-linear model was applied to capture lagged and non-linear exposure effects. We further examined the modifying roles of temperature, humidity, wind speed, and atmospheric pressure, and conducted stratified analyses by sex, age, and season. Results: O₃ exposure was significantly associated with increased cerebral infarction risk, particularly during the warm season. A bimodal temperature-lag pattern was observed, as follows: moderate temperatures (10–20 °C) were associated with immediate effects, while cold (<10 °C) and hot (>30 °C) temperatures were linked to delayed risks. The association of O₃ and hospitalizations for cerebral infarction appeared stronger under high humidity, low wind speed, and low atmospheric pressure. Conclusions: Short-term O₃ exposure and adverse meteorological conditions are jointly associated with an elevated risk of cerebral infarction. Integrated air quality and weather-based warning systems are essential for targeted stroke prevention. Full article

(This article belongs to the Special Issue Ozone Pollution and Adverse Health Impacts)

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27 pages, 3771 KiB

Open AccessArticle

Health Risks from Microplastics in Intravenous Infusions: Evidence from Italy, Spain, and Ecuador

by Claudio Casella, Umberto Cornelli, Giuseppe Zanoni, Pablo Moncayo and Luis Ramos-Guerrero

Toxics 2025, 13(7), 597; https://doi.org/10.3390/toxics13070597 - 16 Jul 2025

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Abstract

The rising incidence of microplastics (MPs) is a possible health risk to humans. The present study aims to analyze the presence of MPs in intravenous (IV) infusions and compare MP concentrations from multiple brands. The IV solutions of 29 medical devices (IV-MDs) from [...] Read more.

The rising incidence of microplastics (MPs) is a possible health risk to humans. The present study aims to analyze the presence of MPs in intravenous (IV) infusions and compare MP concentrations from multiple brands. The IV solutions of 29 medical devices (IV-MDs) from seven brands commercialized in Ecuador, Spain, and Italy have been selected under specific consideration to carry out the study. The detection of MPs has been quite obvious in almost all of the samples from brands in the mentioned countries. MP concentrations ranged from 9 to 20 MPs/L in glass containers to 166–299 MPs/L in plastic bags, with the majority of fragments (63%) on fibres (37%) and more than 60% of particles less than 100 µm. Nine different types of MPs were identified in this study. High clinical risk was indicated by markings with >200 MPs/L. Nevertheless, the medium polymeric danger index (PHI) was 1.7. According to these outcomes, IV infusion is a direct exposure to MPs that may have harmful medical repercussions. It is imperative that MPs’ limitations be included in pharmacopoeic monographs and in vivo toxicological and epidemiological studies. The present study aims to analyze the presence of MPs in IV-MDs and compare MP concentrations from multiple IV-MD brands. Full article

(This article belongs to the Topic Plastic Contamination (Plastamination): An Environmental and Public Health-Related Concern)

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24 pages, 1164 KiB

Open AccessFeature PaperReview

The Aryl Hydrocarbon Receptor in Neurotoxicity: An Intermediator Between Dioxins and Neurons in the Brain

by Eiki Kimura

Toxics 2025, 13(7), 596; https://doi.org/10.3390/toxics13070596 - 16 Jul 2025

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Abstract

Industrial development has increased environmental dioxin concentrations, sparking concern about human health impacts. Examining dioxin neurotoxicity has highlighted associations with cognitive impairment and behavioral abnormality. Dioxins are ligands of the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor; it is speculated that dioxin-induced [...] Read more.

Industrial development has increased environmental dioxin concentrations, sparking concern about human health impacts. Examining dioxin neurotoxicity has highlighted associations with cognitive impairment and behavioral abnormality. Dioxins are ligands of the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor; it is speculated that dioxin-induced AHR activation is pivotal for toxic effects. Accurate AHR-expressing cell identification is therefore indispensable for understanding the molecular and cellular mechanisms of dioxin toxicity. Herein, current knowledge regarding AHR expression in the mammalian brain is summarized, and dioxin neurotoxicity mechanisms are discussed. Histological studies show AHR-expressing neurons in multiple brain regions, including the hippocampus and cerebral cortex. Dopaminergic and noradrenergic neurons exhibit AHR expression, suggesting possible roles in the monoaminergic system. AHR overactivation evokes dendritic arborization atrophy, whereas its deficiency increases complexity, implying that AHR-mediated signaling is crucial for neuronal growth and maturation. AHR is also involved in neurogenesis and neuronal precursor migration. Collectively, these findings support the notion that dioxin-induced AHR overactivation in individual neurons disrupts neural circuit structure, ultimately leading to impaired brain function. However, as AHR downstream signaling is intertwined with various molecules and pathways, the precise mechanisms remain unclear. Further studies on the expression, signaling, and roles of AHR are needed to clarify dioxin neurotoxicity. Full article

(This article belongs to the Section Human Toxicology and Epidemiology)

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13 pages, 933 KiB

Open AccessArticle

Accumulation Patterns and Health Risk Assessment of Trace Elements in Intermuscular Bone-Free Crucian Carp

by Shizhan Tang, Na Li, Zhipeng Sun, Ting Yan, Tingting Zhang, Huan Xu, Zhongxiang Chen, Dongli Qin and Youyi Kuang

Toxics 2025, 13(7), 595; https://doi.org/10.3390/toxics13070595 - 16 Jul 2025

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Abstract

This study investigated the accumulation characteristics and associated health risks of 11 trace elements (Al, Rb, Cr, Ni, Mo, Sr, Pb, Ba, Ag, As, and Ga) in four crucian carp varieties: gene-edited intermuscular bone-free crucian carp (Carassius auratus, WUCI) and its sibling [...] Read more.

This study investigated the accumulation characteristics and associated health risks of 11 trace elements (Al, Rb, Cr, Ni, Mo, Sr, Pb, Ba, Ag, As, and Ga) in four crucian carp varieties: gene-edited intermuscular bone-free crucian carp (Carassius auratus, WUCI) and its sibling wild-type (Carassius auratus, WT), Fangzheng silver crucian carp (Carassius gibelio var Fangzheng, FZYJ), and Songpu silver crucian carp (Carassius gibelio var Songpu, SPYJ). Results showed that Al and Rb were the most abundant elements across all groups. WUCI exhibited distinct accumulation patterns, including significantly higher hepatic Mo concentrations (0.265 ± 0.032 mg/kg) and muscle/liver Rb levels (muscle: 8.74 ± 1.21 mg/kg; liver: 12.56 ± 2.05 mg/kg) compared to other varieties (p < 0.05), which supports the hypothesis of genotype-specific differences in heavy metal accumulation. Correlation analysis revealed that WUCI exhibited similar elemental interactions with WT and SPYJ (e.g., Al-Ni positive correlation, |rs| ≥ 0.8), while SPYJ displayed distinct patterns with fifteen negative correlations compared to three to five in others varieties, suggesting a potential alteration in elemental homeostasis. Pollution index (P_i) assessments indicated mild contamination for Pb in SPYJ liver (P_i = 0.265) and Cr/As in WUCI muscle (P_i = 0.247/0.218). Despite these values, all hazard indices remained below the established safety thresholds (THQ < 0.1, HI < 0.25, TCR < 10⁻⁶), reinforcing the overall safety of the tested fish. Notably, muscle As levels (0.86 ± 0.15 mg/kg) exceeded hepatic concentrations (0.52 ± 0.09 mg/kg), potentially due to differential detoxification mechanisms. These findings demonstrate the food safety of all tested varieties, while highlighting genotype-specific metabolic adaptations, providing critical data for evaluating gene edited aquatic products. Full article

(This article belongs to the Special Issue Effects of Toxic Contaminants on Fish Behaviours)

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18 pages, 4436 KiB

Open AccessArticle

Liraglutide Attenuates Atorvastatin-Induced Hepatotoxicity by Restoring GLP-1R Expression and Activating Nrf2 and Autophagy Pathways in Wistar Rats

by Engy A. Elsiad, Hayat A. Abd El Aal, Hesham A. Salem, Mohammed F. El-Yamany and Mostafa A. Rabie

Toxics 2025, 13(7), 594; https://doi.org/10.3390/toxics13070594 - 16 Jul 2025

Viewed by 284

Abstract

HMG-CoA reductase inhibitors, statins, are extensively used to treat hyperlipidemia, coronary artery disease, and other atherosclerotic disorders. However, one of the common side effects of statin therapy is a mild elevation in liver aminotransferases, observed in less than 3% of patients. Atorvastatin and [...] Read more.

HMG-CoA reductase inhibitors, statins, are extensively used to treat hyperlipidemia, coronary artery disease, and other atherosclerotic disorders. However, one of the common side effects of statin therapy is a mild elevation in liver aminotransferases, observed in less than 3% of patients. Atorvastatin and simvastatin, in particular, are most frequently associated with statin-induced liver injury, leading to treatment discontinuation. Recent research has highlighted the antioxidant and anti-inflammatory properties of glucagon-like peptide-1 receptor (GLP-1R) activation in protecting against liver injury. Nonetheless, the potential protective effects of liraglutide (LIRA), a GLP-1R agonist, against atorvastatin (ATO)-induced liver dysfunction have not been fully elucidated. In this context, the present study aimed to investigate the protective role of LIRA in mitigating ATO-induced liver injury in rats, offering new insights into managing statin-associated hepatotoxicity. Indeed, LIRA treatment improved liver function enzymes and attenuated histopathological alterations. LIRA treatment enhanced antioxidant defenses by increasing Nrf2 content and superoxide dismutase (SOD) activity, while reducing NADPH oxidase. Additionally, LIRA suppressed inflammation by downregulating the HMGB1/TLR-4/RAGE axis and inhibiting the protein expression of pY323-MAPK p38 and pS635-NFκB p65 content resulting in decreased proinflammatory cytokines (TNF-α and IL-1β). Furthermore, LIRA upregulated GLP-1R gene expression and promoted autophagic influx via the activation of the pS473-Akt/pS486-AMPK/pS758-ULK1/Beclin-1 signaling cascade, along with inhibiting apoptosis by reducing caspase-3 content. In conclusion, LIRA attenuated ATO-induced oxidative stress and inflammation via activation of the Nrf-2/SOD cascade and inhibition of the HMGB1/TLR-4/RAGE /MAPK p38/NFκB p65 axis. In parallel, LIRA stimulated autophagy via the AMPK/ULK1/Beclin-1 axis and suppressed apoptosis, thus restoring the balance between autophagy and apoptosis. Full article

(This article belongs to the Section Drugs Toxicity)

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16 pages, 3177 KiB

Open AccessArticle

Cadmium as the Critical Limiting Factor in the Co-Disposal of Municipal Solid Waste Incineration Fly Ash in Cement Kilns: Implications for Three-Stage Water Washing Efficiency and Safe Dosage Control

by Zhonggen Li, Qingfeng Wang, Li Tang, Liangliang Yang and Guangyi Sun

Toxics 2025, 13(7), 593; https://doi.org/10.3390/toxics13070593 - 15 Jul 2025

Viewed by 253

Abstract

The co-disposal of municipal solid waste incineration fly ash (MSWI-FA) in cement kilns is an effective method for managing incineration by-products in China. However, the presence of heavy metals in MSWI-FA raises environmental concerns. This study analyzed the Cu, Zn, Cd, Pb, Cr, [...] Read more.

The co-disposal of municipal solid waste incineration fly ash (MSWI-FA) in cement kilns is an effective method for managing incineration by-products in China. However, the presence of heavy metals in MSWI-FA raises environmental concerns. This study analyzed the Cu, Zn, Cd, Pb, Cr, and Ni concentrations in MSWI-FA from 11 representative facilities across China and assessed the efficacy of a three-stage water washing process for Cl and heavy metal removal. The results revealed significant regional variations in heavy metal content that were strongly correlated with surface soil levels, with Zn, Pb, and Cu exhibiting the highest concentrations. Elemental correlations, such as Cu-Pb and Zn-Cd synergies and Cd-Ni antagonism, suggest common waste sources and temperature-dependent volatilization during incineration. The washing process (solid–liquid ratio = 1:10) achieved 97.1 ± 2.0% Cl removal, reducing residual Cl to 0.45 ± 0.32%, but demonstrated limited heavy metal elimination (10.28–19.38% efficiency), resulting in elevated concentrations (32.5–60.8% increase) due to 43.4 ± 9.2% mass loss. Notably, the washing effluents exceeded municipal wastewater discharge limits by up to 52-fold for Pb and 38-fold for Cd, underscoring the need for advanced effluent treatment. To mitigate environmental risks, the addition of washed MSWI-FA in cement kilns should be restricted to ≤0.5%, with Cd content prioritized in pre-disposal assessments. This study provides actionable insights for optimizing MSWI-FA co-processing while ensuring compliance with ecological safety standards. Full article

(This article belongs to the Special Issue Distribution and Behavior of Trace Metals in the Environment)

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20 pages, 1893 KiB

Open AccessArticle

Acute Dermatotoxicity of Green-Synthesized Silver Nanoparticles (AgNPs) in Zebrafish Epidermis

by Grace Emily Okuthe and Busiswa Siguba

Toxics 2025, 13(7), 592; https://doi.org/10.3390/toxics13070592 - 15 Jul 2025

Viewed by 193

Abstract

Silver nanoparticles (AgNPs), lauded for their unique antibacterial and physicochemical attributes, are proliferating across industrial sectors, raising concerns about their environmental fate, in aquatic systems. While “green” synthesis offers a sustainable production route with reduced chemical byproducts, the safety of these AgNPs for [...] Read more.

Silver nanoparticles (AgNPs), lauded for their unique antibacterial and physicochemical attributes, are proliferating across industrial sectors, raising concerns about their environmental fate, in aquatic systems. While “green” synthesis offers a sustainable production route with reduced chemical byproducts, the safety of these AgNPs for aquatic fauna remains uncertain due to nanoparticle-specific effects. Conversely, mast cells play crucial roles in fish immunity, orchestrating innate and adaptive immune responses by releasing diverse mediators and recognizing danger signals. Goblet cells are vital for mucosal immunity and engaging in immune surveillance, regulation, and microbiota interactions. The interplay between these two cell types is critical for maintaining mucosal homeostasis, is central to defending against fish diseases and is highly responsive to environmental cues. This study investigates the acute dermatotoxicity of environmentally relevant AgNP concentrations (0, 0.031, 0.250, and 5.000 μg/L) on zebrafish epidermis. A 96 h assay revealed a biphasic response: initial mucin hypersecretion at lower AgNP levels, suggesting an early stress response, followed by a concentration-dependent collapse of mucosal integrity at higher exposures, with mucus degradation and alarm cell depletion. A rapid and generalized increase in epidermal mucus production was observed across all AgNP exposure groups within two hours of exposure. Further mechanistic insights into AgNP-induced toxicity were revealed by concentration-dependent alterations in goblet cell dynamics. Lower AgNP concentrations initially led to an increase in both goblet cell number and size. However, at the highest concentration, this trend reversed, with a significant decrease in goblet cell numbers and size evident between 48 and 96 h post-exposure. The simultaneous presence of neutral and acidic mucins indicates a dynamic epidermal response suggesting a primary physical barrier function, with acidic mucins specifically upregulated early on to enhance mucus viscosity, trap AgNPs, and inhibit pathogen invasion, a clear defense mechanism. The subsequent reduction in mucin-producing cells at higher concentrations signifies a critical breakdown of this protective strategy, leaving the epidermis highly vulnerable to damage and secondary infections. These findings highlight the vulnerability of fish epidermal defenses to AgNP contamination, which can potentially compromise osmoregulation and increase susceptibility to threats. Further mechanistic research is crucial to understand AgNP-induced epithelial damage to guide sustainable nanotechnology. Full article

(This article belongs to the Section Ecotoxicology)

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1 pages, 138 KiB

Open AccessCorrection

Correction: Carfora et al. Helium Suicide, a Rapid and Painless Asphyxia: Toxicological Findings. Toxics 2022, 10, 424

by Anna Carfora, Raffaella Petrella, Giusy Ambrosio, Pasquale Mascolo, Bruno Liguori, Christian Juhnke, Carlo Pietro Campobasso and Thomas Keller

Toxics 2025, 13(7), 591; https://doi.org/10.3390/toxics13070591 - 15 Jul 2025

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Abstract

Text Correction [...] Full article

25 pages, 949 KiB

Open AccessFeature PaperArticle

New QSAR Models to Predict Human Transthyretin Disruption by Per- and Polyfluoroalkyl Substances (PFAS): Development and Application

by Marco Evangelista, Nicola Chirico and Ester Papa

Toxics 2025, 13(7), 590; https://doi.org/10.3390/toxics13070590 - 14 Jul 2025

Viewed by 227

Abstract

Per- and polyfluoroalkyl substances (PFAS) are of concern because of their potential thyroid hormone system disruption by binding to human transthyretin (hTTR). However, the amount of experimental data is scarce. In this work, new classification and regression QSARs were developed to predict the [...] Read more.

Per- and polyfluoroalkyl substances (PFAS) are of concern because of their potential thyroid hormone system disruption by binding to human transthyretin (hTTR). However, the amount of experimental data is scarce. In this work, new classification and regression QSARs were developed to predict the hTTR disruption based on experimental data measured for 134 PFAS. Bootstrapping, randomization procedures, and external validation were used to check for overfitting, to avoid random correlations, and to evaluate the predictivity of the QSARs, respectively. The best QSARs were characterized by good performances (e.g., training and test accuracies in classification of 0.89 and 0.85, respectively; R², Q²_loo, and Q²_F3 in regression of 0.81, 0.77, and 0.82, respectively) and significantly broader domains compared to the few existing similar models. The application of QSARs application to the OECD List of PFAS allowed for the identification of structural categories of major concern, such as per- and polyfluoroalkyl ether-based, perfluoroalkyl carbonyl, and perfluoroalkane sulfonyl compounds. Forty-nine PFAS showed a stronger binding affinity to hTTR than the natural ligand T4. Uncertainty quantification for each model and prediction further enhanced the reliability assessment of predictions. The implementation of the new QSARs in non-commercial software facilitates their application to support future research efforts and regulatory actions. Full article

(This article belongs to the Special Issue Computational Toxicology: Exposure and Assessment)

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27 pages, 5816 KiB

Open AccessArticle

Developmental Exposures to Three Mammalian Teratogens Produce Dysmorphic Phenotypes in Adult Caenorhabditis elegans

by Piper Reid Hunt, Martine Ferguson, Nicholas Olejnik, Jeffrey Yourick and Robert L. Sprando

Toxics 2025, 13(7), 589; https://doi.org/10.3390/toxics13070589 - 14 Jul 2025

Viewed by 188

Abstract

Efficient new methods are needed to support initiatives to reduce, refine, and/or replace toxicity testing in vertebrates. 5-fluorouracil (5FU), hydroxyurea (HU), and ribavirin (RV) are mammalian teratogens. Skeletal, endocrine organ, and cardiac effects are often associated with teratogenesis, and a simple nematode like [...] Read more.

Efficient new methods are needed to support initiatives to reduce, refine, and/or replace toxicity testing in vertebrates. 5-fluorouracil (5FU), hydroxyurea (HU), and ribavirin (RV) are mammalian teratogens. Skeletal, endocrine organ, and cardiac effects are often associated with teratogenesis, and a simple nematode like C. elegans lacks these systems. However, many genetic pathways required for mammalian morphogenesis have at least some conserved elements in this small, invertebrate model. The C. elegans lifecycle is 3 days. The effects of 5FU, HU, and RV on the C. elegans morphology were evaluated on day 4 post-initiation of the feeding after hatching for continuous and 24 h (early-only) developmental exposures. Continuous exposures to 5FU and HU induced increases in the incidences of abnormal gonadal structures that were significantly reduced in early-only exposure groups. The incidence of prolapse increased with continuous 5FU and HU exposures and was further increased in early-only exposure groups. Intestinal prolapse through the vulval muscle in C. elegans may be related to reported 5FU and HU effects on skeletal muscle and the gastrointestinal tract in mammals. Continuous RV exposures induced a phenotype lacking a uterus and gonad arms, as well as vulval anomalies that were largely, but not completely, reversed with early-only exposures, which is consistent with reported reversible reproductive tract anomalies after an RV exposure in mammals. These findings suggest that C. elegans can be used to detect the hazard risk from chemicals that adversely affect conserved pathways involved in organismal morphogenesis, but to determine the fit-for-purpose use of this model in chemical safety evaluations, further studies using larger and more diverse chemical test panels are needed. Full article

(This article belongs to the Section Novel Methods in Toxicology Research)

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12 pages, 5245 KiB

Open AccessArticle

Evaluation of Fly Ash Composition from Municipal Solid Waste Incinerators: The Role of the Incinerator Type and Flue Gas Deacidification Process

by Xuetong Qu, Yanan Wang, Feifei Chen, Chuqiao Li, Yunfei He, Jibo Dou, Shuai Zhang, Jiafeng Ding, Hangjun Zhang and Yuchi Zhong

Toxics 2025, 13(7), 588; https://doi.org/10.3390/toxics13070588 - 14 Jul 2025

Viewed by 231

Abstract

The resource utilization potential and environmental impact of fly ash from municipal solid waste incinerators (MSWIs) have attracted wide attention. In this study, four MSWIs in Hangzhou, Zhejiang Province were selected to systematically evaluate the effects of different incinerator types and flue gas [...] Read more.

The resource utilization potential and environmental impact of fly ash from municipal solid waste incinerators (MSWIs) have attracted wide attention. In this study, four MSWIs in Hangzhou, Zhejiang Province were selected to systematically evaluate the effects of different incinerator types and flue gas deacidification processes on fly ash’s oxide and heavy metal components and their temporal changes as well as conduct risk assessment. The results showed that the contents of MgO, Al₂O₃, SiO₂, and Fe₂O₃ in the grate furnace fly ash were significantly lower than those in the fluidized bed fly ash, but the compressive strength of its fly ash was high. Chemicals added during the flue gas deacidification process such as CaO and NaHCO₃ significantly affected the contents of CaO and Na₂O. In addition, heavy metals such as Cu, Mn, Cr, and Ni were mainly distributed in the fluidized bed fly ash, while heavy metals such as Pb and Cd were mainly collected in the grate furnace fly ash. The concentrations of various components in the fly ash fluctuated but were not significant under different time dimensions. Risk assessment indicated that heavy metals such as Cd, Pb, and Sb posed a high risk. This study is expected to provide theoretical support for the safe management and resource utilization of fly ash. Full article

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24 pages, 2639 KiB

Open AccessReview

Cement Industry Pollution and Its Impact on the Environment and Population Health: A Review

by Alina Bărbulescu and Kamal Hosen

Toxics 2025, 13(7), 587; https://doi.org/10.3390/toxics13070587 - 14 Jul 2025

Viewed by 717

Abstract

The cement industry, a foundation of global infrastructure development, significantly contributes to environmental pollution. Key sources of pollution include dust emissions; greenhouse gases, particularly carbon dioxide; and the release of toxic substances such as heavy metals and particulate matter. These pollutants contribute to [...] Read more.

The cement industry, a foundation of global infrastructure development, significantly contributes to environmental pollution. Key sources of pollution include dust emissions; greenhouse gases, particularly carbon dioxide; and the release of toxic substances such as heavy metals and particulate matter. These pollutants contribute to air, water, and soil degradation and are linked to severe health conditions in nearby populations, including respiratory disorders, cardiovascular diseases, and increased mortality rates. Noise pollution is also a significant issue, inducing auditory diseases that affect most workers in cement plants, and disturbing the population living in the neighborhoods and fauna behavior. This review explores the pollution paths and the multifaceted impacts of cement production on the environment. It also highlights the social challenges faced by communities, underscoring the urgent need for stricter environmental policies and the adoption of greener technologies to mitigate the adverse effects of cement production on both the environment and human health. Full article

(This article belongs to the Special Issue Exposure to Chemicals and Health Effects in Occupational and Everyday Settings)

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22 pages, 3768 KiB

Open AccessArticle

MWB_Analyzer: An Automated Embedded System for Real-Time Quantitative Analysis of Morphine Withdrawal Behaviors in Rodents

by Moran Zhang, Qianqian Li, Shunhang Li, Binxian Sun, Zhuli Wu, Jinxuan Liu, Xingchao Geng and Fangyi Chen

Toxics 2025, 13(7), 586; https://doi.org/10.3390/toxics13070586 - 14 Jul 2025

Viewed by 315

Abstract

Background/Objectives: Substance use disorders, particularly opioid addiction, continue to pose a major global health and toxicological challenge. Morphine dependence represents a significant problem in both clinical practice and preclinical research, particularly in modeling the pharmacodynamics of withdrawal. Rodent models remain indispensable for investigating [...] Read more.

Background/Objectives: Substance use disorders, particularly opioid addiction, continue to pose a major global health and toxicological challenge. Morphine dependence represents a significant problem in both clinical practice and preclinical research, particularly in modeling the pharmacodynamics of withdrawal. Rodent models remain indispensable for investigating the neurotoxicological effects of chronic opioid exposure and withdrawal. However, conventional behavioral assessments rely on manual observation, limiting objectivity, reproducibility, and scalability—critical constraints in modern drug toxicity evaluation. This study introduces MWB_Analyzer, an automated and high-throughput system designed to quantitatively and objectively assess morphine withdrawal behaviors in rats. The goal is to enhance toxicological assessments of CNS-active substances through robust, scalable behavioral phenotyping. Methods: MWB_Analyzer integrates optimized multi-angle video capture, real-time signal processing, and machine learning-driven behavioral classification. An improved YOLO-based architecture was developed for the accurate detection and categorization of withdrawal-associated behaviors in video frames, while a parallel pipeline processed audio signals. The system incorporates behavior-specific duration thresholds to isolate pharmacologically and toxicologically relevant behavioral events. Experimental animals were assigned to high-dose, low-dose, and control groups. Withdrawal was induced and monitored under standardized toxicological protocols. Results: MWB_Analyzer achieved over 95% reduction in redundant frame processing, markedly improving computational efficiency. It demonstrated high classification accuracy: >94% for video-based behaviors (93% on edge devices) and >92% for audio-based events. The use of behavioral thresholds enabled sensitive differentiation between dosage groups, revealing clear dose–response relationships and supporting its application in neuropharmacological and neurotoxicological profiling. Conclusions: MWB_Analyzer offers a robust, reproducible, and objective platform for the automated evaluation of opioid withdrawal syndromes in rodent models. It enhances throughput, precision, and standardization in addiction research. Importantly, this tool supports toxicological investigations of CNS drug effects, preclinical pharmacokinetic and pharmacodynamic evaluations, drug safety profiling, and regulatory assessment of novel opioid and CNS-active therapeutics. Full article

(This article belongs to the Section Drugs Toxicity)

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16 pages, 4613 KiB

Open AccessArticle

Perfluorononanoic Acid (PFNA) Exacerbates Atopic Dermatitis by Inducing Inflammation in Mice

by Jiali Xiao, Junchao Wang, Nuo Xu, Xulong Huang, Farid Khalilov, Xianfeng Huang, Xiangyong Zheng, Xiashun Xu, Shisheng Lin, Wengang Zhao and Elchin Khalilov

Toxics 2025, 13(7), 585; https://doi.org/10.3390/toxics13070585 - 13 Jul 2025

Viewed by 350

Abstract

Perfluorononanoic acid (PFNA) is a ubiquitous persistent environmental pollutant, and several studies have found significant links between atopic dermatitis (AD) and prenatal exposure to PFNA. However, the relationship between PFNA and AD remains unclear. In this study, 2,4-dinitrochlorobenzene (DNCB)-treated female BALB/c mice were [...] Read more.

Perfluorononanoic acid (PFNA) is a ubiquitous persistent environmental pollutant, and several studies have found significant links between atopic dermatitis (AD) and prenatal exposure to PFNA. However, the relationship between PFNA and AD remains unclear. In this study, 2,4-dinitrochlorobenzene (DNCB)-treated female BALB/c mice were used as AD models to investigate the effects of PFNA and its potential mechanisms. These mice were topically applied with 5 mg/kg PFNA per day for 15 days. The results demonstrated that PFNA significantly increased AD lesion severity and clinical symptoms, including dermatitis score, ear thickness, and epidermal thickness. In addition, PFNA also increased the serum IgE level, splenic atrophy, and upregulated the expression of TNF-α, IL-6, and IL-1β, genes that are associated with skin inflammatory factors. In addition, Western blot results showed that PFNA treatment upregulated the expression of p-JNK protein. Additionally, cellular experiments indicated that RAW264.7 macrophages and mouse brain microvascular endothelial (bEnd.3) cells treated with PFNA at concentrations of 0.01–100 μM for 72 h showed no changes in cell viability. However, 100 μM PFNA upregulated the mRNA expression levels of the pro-inflammatory cytokines IL-1β and IL-6, as well as the protein expression of p-JNK, in RAW264.7 cells induced with 1 mg/mL LPS for 2 h. Similarly, PFNA increased TNF-α and IL-6 mRNA expression and p-JNK protein expression in bEnd.3 cells stimulated with 20 ng/mL TNF-α for 0.5 h. Based on these findings, we can conclude that PFNA may aggravate atopic dermatitis by promoting inflammation. Full article

(This article belongs to the Section Emerging Contaminants)

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16 pages, 4256 KiB

Open AccessArticle

Physiological Stress Responses Associated with Microplastic Ingestion in the Benthic Flatfish Bothus podas

by Amanda Cohen-Sánchez, Montserrat Compa, Jessica Lombardo, Maria Magdalena Quetglas-Llabrés, Maria del Mar Ribas-Taberner, Manuel Jiménez-García, Silvia Tejada and Antoni Sureda

Toxics 2025, 13(7), 584; https://doi.org/10.3390/toxics13070584 - 13 Jul 2025

Viewed by 479

Abstract

Bothus podas (wide-eyed flounder) is a benthic flatfish likely exposed to microplastic (MP) pollution. We investigated MP ingestion and associated physiological effects in wild B. podas collected from Mallorca (Balearic Islands), Spain. Markers of oxidative stress, detoxification, and immunity were quantified in intestinal, [...] Read more.

Bothus podas (wide-eyed flounder) is a benthic flatfish likely exposed to microplastic (MP) pollution. We investigated MP ingestion and associated physiological effects in wild B. podas collected from Mallorca (Balearic Islands), Spain. Markers of oxidative stress, detoxification, and immunity were quantified in intestinal, hepatic, and splenic tissues. MPs were observed in the gastrointestinal tracts of 87.5% of the 24 specimens analyzed, with an average of 3.8 ± 0.6 items per fish. Fiber-type MPs predominated in both the gastrointestinal tract (69.6%) and sediment samples (97%). Additionally, micro-Fourier transform infrared spectroscopy analysis confirmed that the majority of ingested MPs were composed of polyethylene, polypropylene, and polyester. Fish were categorized into low (<3 items) and high (≥3 items) MP groups based on the median number of plastic items found in the gastrointestinal tract to assess sublethal impacts. In the gut, high-MP fish exhibited significantly elevated activities of detoxification enzymes: ethoxyresorufin-O-deethylase (phase I) and glutathione s-transferase (phase II), along with increased antioxidant enzyme superoxide dismutase and inflammatory myeloperoxidase. Gut catalase and malondialdehyde (MDA) were not significantly different between groups. In liver tissues, no biomarkers differed significantly with MP exposure. In the spleen, lysozyme and alkaline phosphatase activities were significantly higher in high-MP fish, while splenic MDA remained unchanged. These results indicate that gastrointestinal MP exposure triggers local oxidative stress responses and systemic immune activation in B. podas. Overall, ingestion of environmentally relevant MP levels elicited detoxification and inflammatory responses without significant increases in MDA, an indicator of oxidative damage, highlighting the physiological stress imposed by plastic pollution on benthic fish. Full article

(This article belongs to the Special Issue Microplastic Pollution and Impact on Aquatic and Terrestrial Ecosystems)

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13 pages, 1084 KiB

Open AccessArticle

Airborne SARS-CoV-2 Detection by ddPCR in Adequately Ventilated Hospital Corridors

by Joan Truyols-Vives, Marta González-López, Antoni Colom-Fernández, Alexander Einschütz-López, Ernest Sala-Llinàs, Antonio Doménech-Sánchez, Herme García-Baldoví and Josep Mercader-Barceló

Toxics 2025, 13(7), 583; https://doi.org/10.3390/toxics13070583 - 12 Jul 2025

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Abstract

Indoors, the infection risk of diseases transmitted through the airborne route is estimated from indoor carbon dioxide (CO₂) levels. However, the approaches to assess this risk do not account for the airborne concentration of pathogens, among other limitations. In this study, [...] Read more.

Indoors, the infection risk of diseases transmitted through the airborne route is estimated from indoor carbon dioxide (CO₂) levels. However, the approaches to assess this risk do not account for the airborne concentration of pathogens, among other limitations. In this study, we analyzed the relationship between airborne SARS-CoV-2 levels and environmental parameters. Bioaerosols were sampled (n = 40) in hospital corridors of two wards differing in the COVID-19 severity of the admitted patients. SARS-CoV-2 levels were quantified using droplet digital PCR. SARS-CoV-2 was detected in 60% of the total air samples. The ward where the mildly ill patients were admitted had a higher occupancy, transit of people in the corridor, and CO₂ levels, but there were no significant differences in SARS-CoV-2 detection between wards. The mean CO₂ concentration in the positive samples was 569 ± 35.6 ppm. Considering all samples, the CO₂ levels in the corridor were positively correlated with patient door openings but inversely correlated with SARS-CoV-2 levels. In conclusion, airborne SARS-CoV-2 can be detected indoors with optimal ventilation, and its levels do not scale with CO₂ concentration in hospital corridors. Therefore, CO₂ assessment should not be interpreted as a surrogate of airborne viral presence in all indoor spaces. Full article

(This article belongs to the Special Issue Cutting-Edge Research in Controlling Aerosols, Improving Public Health)

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13 pages, 1132 KiB

Open AccessArticle

Evaluating the Cytotoxic, Genotoxic, and Toxic Potential of Pyrolytic Tire Char Using Human Lymphocytes and a Bacterial Biosensor

by Ioanna Efthimiou, Margarita Dormousoglou, Lambrini Giova, Dimitris Vlastos, Stefanos Dailianis, Maria Antonopoulou and Ioannis Konstantinou

Toxics 2025, 13(7), 582; https://doi.org/10.3390/toxics13070582 - 12 Jul 2025

Viewed by 264

Abstract

Waste tires (WTs) constitute a potentially significant source of pollution, and the large quantities that are disposed of require proper handling. Pyrolysis has emerged as an environmentally friendly and effective method for WT treatment. In the present study, the cyto-genotoxic and toxic effects [...] Read more.

Waste tires (WTs) constitute a potentially significant source of pollution, and the large quantities that are disposed of require proper handling. Pyrolysis has emerged as an environmentally friendly and effective method for WT treatment. In the present study, the cyto-genotoxic and toxic effects of untreated and acid-treated pyrolytic tire char (PTC_UN and PTC_AT, respectively) were investigated. The cytokinesis block micronucleus (CBMN) assay, using human lymphocytes, and the Aliivibrio fischeri bioluminescence assay were used for the assessment of cyto-genotoxicity and ecotoxicity, respectively. According to the results, both PTC_UN and PTC_AT exhibited genotoxicity at all concentrations tested (2.5, 5, and 10 μg mL⁻¹), which was more pronounced in the case of PTC_AT. Cytotoxicity induction was reported for PTC_UN and PTC_AT at all concentrations. Both demonstrated a relatively low potential for ecotoxicity induction against A. fischeri. Since the cyto-genotoxic and toxic effects of PTC_AT seemed to be more pronounced, the toxic profile of tire char should be investigated in depth before selecting the appropriate applications, thereby avoiding detrimental effects in the environment and humans alike. Full article

(This article belongs to the Special Issue From Low-Tier to Individual Effects of Emerging Pollutants: Integrative Approaches to Ecotoxicological Assessments—2nd Edition)

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18 pages, 1650 KiB

Open AccessArticle

Biomonitoring of Inorganic Pollutants in Blood Samples of Population Affected by the Tajogaite Eruption: The ISVOLCAN Study in Spain

by Katherine Simbaña-Rivera, María Cristo Rodríguez-Pérez, Manuel Enrique Fuentes-Ferrer, Manuel Zumbado Peña, Ángel Rodríguez Hernández, Julia Eychenne, Lucie Sauzéat, Damary S. Jaramillo-Aguilar, Ana Rodríguez Chamorro and Luis D. Boada

Toxics 2025, 13(7), 581; https://doi.org/10.3390/toxics13070581 - 10 Jul 2025

Viewed by 257

Abstract

Volcanic eruptions release gases and particulates that may adversely affect human health. The Tajogaite eruption on La Palma provided a unique opportunity to evaluate inorganic pollutant exposure in a directly affected population. As part of the ISVOLCAN study, blood samples from 393 adults [...] Read more.

Volcanic eruptions release gases and particulates that may adversely affect human health. The Tajogaite eruption on La Palma provided a unique opportunity to evaluate inorganic pollutant exposure in a directly affected population. As part of the ISVOLCAN study, blood samples from 393 adults residing in the island’s western region were analyzed for 43 inorganic elements using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), including 20 toxic elements identified by the Agency for Toxic Substances and Disease Registry (ATSDR). The median age of participants was 51 years, and 56.7% were female. Higher levels of Hg and Mn were associated with long-term occupational exposure, while smoking was linked to elevated Cd, Pb, and Sr levels. Participants living within 6.5 km of the volcano had significantly higher concentrations of Al and Ti. Ash cleanup activities were associated with increased levels of Ni and Cu, and those spending over five hours outdoors daily showed elevated Se and Pb. This is the first biomonitoring study to assess blood concentrations of inorganic pollutants in a population exposed to volcanic emissions. The findings highlight key exposure factors and underscore the need for continued research to assess long-term health effects and inform public health measures. Full article

(This article belongs to the Special Issue Human Biomonitoring in Health Risk Assessment of Emerging Chemicals)

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19 pages, 2183 KiB

Open AccessSystematic Review

Mercury Scenario in Fish from the Amazon Basin: Exploring the Interplay of Social Groups and Environmental Diversity

by Thaís de Castro Paiva, Inácio Abreu Pestana, Lorena Nascimento Leite Miranda, Gabriel Oliveira de Carvalho, Wanderley Rodrigues Bastos and Daniele Kasper

Toxics 2025, 13(7), 580; https://doi.org/10.3390/toxics13070580 - 10 Jul 2025

Viewed by 366

Abstract

The Amazon faces significant challenges related to mercury contamination, including naturally elevated concentrations and gold mining activities. Due to mercury’s toxicity and the importance of fish as a protein source for local populations, assessing mercury levels in regional fish is crucial. However, there [...] Read more.

The Amazon faces significant challenges related to mercury contamination, including naturally elevated concentrations and gold mining activities. Due to mercury’s toxicity and the importance of fish as a protein source for local populations, assessing mercury levels in regional fish is crucial. However, there are gaps in knowledge regarding mercury concentrations in many areas of the Amazon basin. This study aims to synthesize the existing literature on mercury concentrations in fish and the exposure of urban and traditional social groups through fish consumption. A systematic review (1990–2022) was conducted for six fish genera (Cichla spp., Hoplias spp. and Plagioscion spp., Leporinus spp., Semaprochilodus spp., and Schizodon spp.) in the Web of Science (Clarivate Analytics) and Scopus (Elsevier) databases. The database consisted of a total of 46 studies and 455 reports. The distribution of studies in the region was not homogeneous. The most studied regions were the Madeira River sub-basin, while the Paru–Jari basin had no studies. Risk deterministic and probabilistic assessments based on Joint FAO/WHO Expert Committee on Food Additives (JECFA, 2007) guidelines showed high risk exposure, especially for traditional communities. Carnivorous fish from lakes and hydroelectric reservoirs, as well as fish from black-water ecosystems, exhibited higher mercury concentrations. In the Amazon region, even if mercury levels in fish muscle do not exceed regulatory limits, the high fish consumption can still elevate health risks for local populations. Monitoring mercury levels across a broader range of fish species, including both carnivorous and non-carnivorous species, especially in communities heavily reliant on fish for their diet, will enable a more accurate risk assessment and provide an opportunity to recommend fish species with lower mercury exposure risk for human consumption. The present study emphasizes the need to protect regions that already exhibit higher levels of mercury—such as lakes, hydroelectric reservoirs, and black-water ecosystems—to ensure food safety and safeguard public health. Full article

(This article belongs to the Special Issue Mercury Cycling and Health Effects—2nd Edition)

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20 pages, 4637 KiB

Open AccessArticle

Interpretable Machine Learning Models and Symbolic Regressions Reveal Transfer of Per- and Polyfluoroalkyl Substances (PFASs) in Plants: A New Small-Data Machine Learning Method to Augment Data and Obtain Predictive Equations

by Yuan Zhang, Yanting Li, Yang Li, Lin Zhao and Yongkui Yang

Toxics 2025, 13(7), 579; https://doi.org/10.3390/toxics13070579 - 10 Jul 2025

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Abstract

Machine learning (ML) techniques are becoming increasingly valuable for modeling the transport of pollutants in plant systems. However, two challenges (small sample sizes and a lack of quantitative calculation functions) remain when using ML to predict migration in hydroponic systems. For the bioaccumulation [...] Read more.

Machine learning (ML) techniques are becoming increasingly valuable for modeling the transport of pollutants in plant systems. However, two challenges (small sample sizes and a lack of quantitative calculation functions) remain when using ML to predict migration in hydroponic systems. For the bioaccumulation of per- and polyfluoroalkyl substances, we studied the key factors and quantitative calculation equations based on data augmentation, ML, and symbolic regression. First, feature expansion was performed on the input data after data preprocessing; the most important step was data augmentation. The original training set was expanded nine times by combining the synthetic minority oversampling technique and a variational autoencoder. Subsequently, the four ML models were applied to the test set to predict the selected output parameters. Categorical boosting (CatBoost) had the highest prediction accuracy (R² = 0.83). The Shapley Additive Explanation values indicated that molecular weight and exposure time were the most important parameters. We applied three symbolic regression models to obtain accurate prediction equations based on the original and augmented data. Based on augmented data, the high-dimensional sparse interaction equation exhibited the highest accuracy (R² = 0.776). Our results indicate that this method could provide crucial insights into absorption and accumulation in plant roots. Full article

(This article belongs to the Special Issue Transformation Process and Toxic Effects of Pollutants in Agricultural Environment)

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Toxics, Volume 13, Issue 7 (July 2025) – 92 articles

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