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Volume 12
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10.3390/plants12132543
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Article

Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb.

by
Marta Ruiz
1,*,
Annie Du Vo
1,
J. Ole Becker
2 and
Mikeal L. Roose
1,*
1
Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
2
Department of Nematology, University of California, Riverside, CA 92521, USA
*
Authors to whom correspondence should be addressed.
Plants 2023, 12(13), 2543; https://doi.org/10.3390/plants12132543
Submission received: 30 May 2023 / Revised: 27 June 2023 / Accepted: 28 June 2023 / Published: 4 July 2023
(This article belongs to the Special Issue Advances in Breeding, Genetics, and Genomics of Citrus)
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Abstract

As pest management options, such as nematicides, become more restrictive, developing rootstocks resistant to the citrus nematode is fundamental for citrus production. This study provides an updated methodology to screen for citrus nematode resistance in rootstock-breeding programs. We developed a novel method to extract female citrus nematodes from roots that is suitable for molecular work and a real-time-PCR-based nematode quantification method for Tylenchulus semipenetrans. These procedures allow scaling up screening to high-throughput workflows, increasing the chances of finding rootstock candidates that combine all the desired traits. Our results contribute to the growing literature supporting quantification of nematodes with molecular methods.
Keywords: plant parasitic; rootstock; breeding; Poncirus; trifoliate; extraction; quantification; qPCR; screening; pest plant parasitic; rootstock; breeding; Poncirus; trifoliate; extraction; quantification; qPCR; screening; pest

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MDPI and ACS Style

Ruiz, M.; Vo, A.D.; Becker, J.O.; Roose, M.L. Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb. Plants 2023, 12, 2543. https://doi.org/10.3390/plants12132543

AMA Style

Ruiz M, Vo AD, Becker JO, Roose ML. Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb. Plants. 2023; 12(13):2543. https://doi.org/10.3390/plants12132543

Chicago/Turabian Style

Ruiz, Marta, Annie Du Vo, J. Ole Becker, and Mikeal L. Roose. 2023. "Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb." Plants 12, no. 13: 2543. https://doi.org/10.3390/plants12132543

APA Style

Ruiz, M., Vo, A. D., Becker, J. O., & Roose, M. L. (2023). Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb. Plants, 12(13), 2543. https://doi.org/10.3390/plants12132543

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