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Article

Quantitative Analysis of Rutin by HPTLC and In Vitro Antioxidant and Antibacterial Activities of Phenolic-Rich Extracts from Verbesina sphaerocephala

by
Kathia Yanelly Rodríguez-Valdovinos
1,
Rafael Salgado-Garciglia
2,
Monserrat Vázquez-Sánchez
3,
Dioselina Álvarez-Bernal
1,
Ernesto Oregel-Zamudio
1,4,
Luis Fernando Ceja-Torres
1 and
José Roberto Medina-Medrano
5,*
1
Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Michoacán, Instituto Politécnico Nacional, Jiquilpan 59510, Michoacan, Mexico
2
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Michoacan, Mexico
3
Programa de Posgrado en Botánica, Colegio de Postgraduados Campus Montecillo, Texcoco 56230, Estado de Mexico, Mexico
4
Programa de Doctorado en Ciencias en Bioprocesos, Unidad Profesional Interdisciplinaria de Biotecnología (UPIBI), Instituto Politécnico Nacional, Ticomán 07340, Ciudad de Mexico, Mexico
5
CONACYT—Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Michoacán, Instituto Politécnico Nacional, Jiquilpan 59510, Michoacan, Mexico
*
Author to whom correspondence should be addressed.
Plants 2021, 10(3), 475; https://doi.org/10.3390/plants10030475
Submission received: 10 February 2021 / Revised: 25 February 2021 / Accepted: 27 February 2021 / Published: 3 March 2021
(This article belongs to the Special Issue Antioxidant Activity of Medical Plants)
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Abstract

Verbesina sphaerocephala A. Gray, like other wild plants of the genus Verbesina, has been used in herbal medicine. There is information for other species of the genus related to their phenolic content, antioxidant capacity, and isolation of bioactive compounds with antimicrobial activity. However, there are no reports for V. sphaerocephala, although it has an important presence in the state of Michoacán, México. In this study, the phenolic composition, quantification of rutin, and in vitro antioxidant and antibacterial activities of methanolic extracts from V. sphaerocephala leaves and flowers were determined. The results showed that all the investigated extracts have high phenolic and flavonoid contents. The flavonoid rutin was identified in all the extracts from V. sphaerocephala by high-performance thin-layer chromatography (HPTLC). The V. sphaerocephala extracts showed scavenging activity against DPPH• and ABTS•+ radicals (IC50 and 5.83 ± 0.50 and 0.93 ± 0.01 mg/mL, respectively) as well as relevant antioxidant capacity (51.05 ± 0.36 mg of ascorbic acid/g of dry tissue). The experimental results show that V. sphaerocephala extracts possessed a strong antimicrobial activity against Escherichia coli and Staphylococcus aureus bacteria. This research indicates that V. sphaerocephala could be considered as a potential source of natural compounds from the point of ethnopharmacological usage.
Keywords: phenolic compounds; antimicrobial activity; scavenging activity; high-performance thin-layer chromatography (HPTLC); rutin phenolic compounds; antimicrobial activity; scavenging activity; high-performance thin-layer chromatography (HPTLC); rutin
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MDPI and ACS Style

Rodríguez-Valdovinos, K.Y.; Salgado-Garciglia, R.; Vázquez-Sánchez, M.; Álvarez-Bernal, D.; Oregel-Zamudio, E.; Ceja-Torres, L.F.; Medina-Medrano, J.R. Quantitative Analysis of Rutin by HPTLC and In Vitro Antioxidant and Antibacterial Activities of Phenolic-Rich Extracts from Verbesina sphaerocephala. Plants 2021, 10, 475. https://doi.org/10.3390/plants10030475

AMA Style

Rodríguez-Valdovinos KY, Salgado-Garciglia R, Vázquez-Sánchez M, Álvarez-Bernal D, Oregel-Zamudio E, Ceja-Torres LF, Medina-Medrano JR. Quantitative Analysis of Rutin by HPTLC and In Vitro Antioxidant and Antibacterial Activities of Phenolic-Rich Extracts from Verbesina sphaerocephala. Plants. 2021; 10(3):475. https://doi.org/10.3390/plants10030475

Chicago/Turabian Style

Rodríguez-Valdovinos, Kathia Yanelly, Rafael Salgado-Garciglia, Monserrat Vázquez-Sánchez, Dioselina Álvarez-Bernal, Ernesto Oregel-Zamudio, Luis Fernando Ceja-Torres, and José Roberto Medina-Medrano. 2021. "Quantitative Analysis of Rutin by HPTLC and In Vitro Antioxidant and Antibacterial Activities of Phenolic-Rich Extracts from Verbesina sphaerocephala" Plants 10, no. 3: 475. https://doi.org/10.3390/plants10030475

APA Style

Rodríguez-Valdovinos, K. Y., Salgado-Garciglia, R., Vázquez-Sánchez, M., Álvarez-Bernal, D., Oregel-Zamudio, E., Ceja-Torres, L. F., & Medina-Medrano, J. R. (2021). Quantitative Analysis of Rutin by HPTLC and In Vitro Antioxidant and Antibacterial Activities of Phenolic-Rich Extracts from Verbesina sphaerocephala. Plants, 10(3), 475. https://doi.org/10.3390/plants10030475

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