Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L.

Lashin, Islam; Fouda, Amr; Gobouri, Adil A.; Azab, Ehab; Mohammedsaleh, Zuhair M.; Makharita, Rabab R.

doi:10.3390/biom11030341

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Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L.

^1,2,

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³,

⁴

Zuhair M. Mohammedsaleh

⁵

and

Rabab R. Makharita

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Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt

Department of Biology, Faculty of Science and Arts, Al-Mandaq Al-Baha University, Al-Baha 1988, Saudi Arabia

Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

⁴

Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

⁵

Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia

⁶

Biology Department, Faculty of Science and Arts, Khulais, University of Jeddah, Jeddah 21959, Saudi Arabia

⁷

Botany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt

Author to whom correspondence should be addressed.

Academic Editor: Simona Clichici

Biomolecules 2021, 11(3), 341; https://doi.org/10.3390/biom11030341

Received: 29 January 2021 / Revised: 10 February 2021 / Accepted: 18 February 2021 / Published: 24 February 2021

(This article belongs to the Special Issue Bionanomaterials: Synthesis, Characterization, Toxicity and Biomedical Applications)

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Abstract

The in vitro callus induction of Solanum incanum L. was executed on MS medium supplemented with different concentrations of auxin and cytokinin utilizing petioles and explants of leaves. The highest significant fresh weights from petioles and leaf explants were 4.68 and 5.13 g/jar for the medium supplemented with1.0 mg L⁻¹ BA and 1.0 mg L⁻¹ 2,4-D. The callus extract of the leaves was used for the green synthesis of silver nanoparticles (Ag-NPs). Analytical methods used for Ag-NPs characterization were UV-vis spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and Transmission Electron Microscopy (TEM). Spherical, crystallographic Ag-NPs with sizes ranging from 15 to 60nm were successfully formed. The FT-IR spectra exhibited the role of the metabolites involved in callus extract in reducing and capping Ag-NPs. The biological activities of Ag-NPs were dose-dependent. The MIC value for Staphylococcus aureus, Bacillus subtilis, and Escherichia coli was 12.5 µg mL⁻¹, while it was 6.25 µg mL⁻¹ for Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans. The highest inhibition of phytopathogenic fungi Alternaria alternata, Fusarium oxysporum, Aspergillus niger, and Pythium ultimum was 76.3 ± 3.7, 88.9 ± 4.1, 67.8 ± 2.1, and 76.4 ± 1.0%, respectively at 200 µg mL⁻¹. Moreover, green synthesized Ag-NPs showed cytotoxic efficacy against cancerous cell lines HepG2, MCF-7 and normal Vero cell line with IC₅₀ values of 21.76 ± 0.56, 50.19 ± 1.71, and 129.9 ± 0.94 µg mL⁻¹, respectively. View Full-Text

Keywords: Solanum incanum L.; silver nanoparticles; callus aqueous extract; antimicrobial; phytopathogenic fungi; in vitro cytotoxicity

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Lashin, I.; Fouda, A.; Gobouri, A.A.; Azab, E.; Mohammedsaleh, Z.M.; Makharita, R.R. Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L. Biomolecules 2021, 11, 341. https://doi.org/10.3390/biom11030341

AMA Style

Lashin I, Fouda A, Gobouri AA, Azab E, Mohammedsaleh ZM, Makharita RR. Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L. Biomolecules. 2021; 11(3):341. https://doi.org/10.3390/biom11030341

Chicago/Turabian Style

Lashin, Islam, Amr Fouda, Adil A. Gobouri, Ehab Azab, Zuhair M. Mohammedsaleh, and Rabab R. Makharita. 2021. "Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L." Biomolecules 11, no. 3: 341. https://doi.org/10.3390/biom11030341

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Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L.

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