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Multiple Screening of Pesticides Toxicity in Zebrafish and Daphnia Based on Locomotor Activity Alterations

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Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 320314, Taiwan
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Department of Chemistry, Chung Yuan Christian University, Chung-Li 320314, Taiwan
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Department of Biomedical Engineering, Chung Yuan Christian University, Chung-Li 320314, Taiwan
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Department of Applied Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Thanyaburi 12110, Thailand
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Department of Biological Science & Technology, College of Medicine, I-Shou University, Kaohsiung 82445, Taiwan
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Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan
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Department of Applied Chemistry, National Pingtung University, Pingtung 900391, Taiwan
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Department of Aquatic Biosciences, National Chiayi University, 300 University Rd., Chiayi 60004, Taiwan
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Center of Nanotechnology, Chung Yuan Christian University, Chung-Li 320314, Taiwan
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Authors to whom correspondence should be addressed.
Equal contribution authors.
Biomolecules 2020, 10(9), 1224; https://doi.org/10.3390/biom10091224
Received: 29 June 2020 / Revised: 17 August 2020 / Accepted: 20 August 2020 / Published: 23 August 2020
Pesticides are widely used to eradicate insects, weed species, and fungi in agriculture. The half-lives of some pesticides are relatively long and may have the dire potential to induce adverse effects when released into the soil, terrestrial and aquatic systems. To assess the potential adverse effects of pesticide pollution in the aquatic environment, zebrafish (Danio rerio) and Daphnia magna are two excellent animal models because of their transparent bodies, relatively short development processes, and well-established genetic information. Moreover, they are also suitable for performing high-throughput toxicity assays. In this study, we used both zebrafish larvae and water flea daphnia neonates as a model system to explore and compare the potential toxicity by monitoring locomotor activity. Tested animals were exposed to 12 various types of pesticides (three fungicides and 9 insecticides) for 24 h and their corresponding locomotor activities, in terms of distance traveled, burst movement, and rotation were quantified. By adapting principal component analysis (PCA) and hierarchical clustering analysis, we were able to minimize data complexity and compare pesticide toxicity based on locomotor activity for zebrafish and daphnia. Results showed distinct locomotor activity alteration patterns between zebrafish and daphnia towards pesticide exposure. The majority of pesticides tested in this study induced locomotor hypo-activity in daphnia neonates but triggered locomotor hyper-activity in zebrafish larvae. According to our PCA and clustering results, the toxicity for 12 pesticides was grouped into two major groups based on all locomotor activity endpoints collected from both zebrafish and daphnia. In conclusion, all pesticides resulted in swimming alterations in both animal models by either producing hypo-activity, hyperactivity, or other changes in swimming patterns. In addition, zebrafish and daphnia displayed distinct sensitivity and response against different pesticides, and the combinational analysis approach by using a phenomic approach to combine data collected from zebrafish and daphnia provided better resolution for toxicological assessment. View Full-Text
Keywords: zebrafish; daphnia; pesticide; locomotion; behavior; phenomics zebrafish; daphnia; pesticide; locomotion; behavior; phenomics
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MDPI and ACS Style

Hussain, A.; Audira, G.; Malhotra, N.; Uapipatanakul, B.; Chen, J.-R.; Lai, Y.-H.; Huang, J.-C.; Chen, K.H.-C.; Lai, H.-T.; Hsiao, C.-D. Multiple Screening of Pesticides Toxicity in Zebrafish and Daphnia Based on Locomotor Activity Alterations. Biomolecules 2020, 10, 1224. https://doi.org/10.3390/biom10091224

AMA Style

Hussain A, Audira G, Malhotra N, Uapipatanakul B, Chen J-R, Lai Y-H, Huang J-C, Chen KH-C, Lai H-T, Hsiao C-D. Multiple Screening of Pesticides Toxicity in Zebrafish and Daphnia Based on Locomotor Activity Alterations. Biomolecules. 2020; 10(9):1224. https://doi.org/10.3390/biom10091224

Chicago/Turabian Style

Hussain, Akhlaq, Gilbert Audira, Nemi Malhotra, Boontida Uapipatanakul, Jung-Ren Chen, Yu-Heng Lai, Jong-Chin Huang, Kelvin H.-C. Chen, Hong-Thih Lai, and Chung-Der Hsiao. 2020. "Multiple Screening of Pesticides Toxicity in Zebrafish and Daphnia Based on Locomotor Activity Alterations" Biomolecules 10, no. 9: 1224. https://doi.org/10.3390/biom10091224

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