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Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development

Department of Physiology, Medical University of Bialystok, 15-089 Bialystok, Poland
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Biomolecules 2020, 10(8), 1133; https://doi.org/10.3390/biom10081133
Received: 15 June 2020 / Revised: 25 July 2020 / Accepted: 29 July 2020 / Published: 31 July 2020
Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease characterized by excessive lipid deposition. Lipid metabolism disturbances are possibly associated with hepatocyte inflammation development and oxidative balance impairment. The aim of our experiment was to examine the first moment when changes in plasma and liver arachidonic acid (AA) levels as a pro-inflammatory precursor may occur during high-fat diet (HFD)-induced NAFLD development. Wistar rats were fed a diet rich in fat for five weeks, and after each week, inflammation and redox balance parameters were evaluated in the liver. The AA contents in lipid fractions were assessed by gas–liquid chromatography (GLC). Protein expression relevant to inflammatory and lipogenesis pathways was determined by immunoblotting. The oxidative system indicators were determined with assay kits. Our results revealed that a high-fat diet promoted an increase in AA levels, especially in the phospholipid (PL) fraction. Importantly, rapid inflammation development via increased inflammatory enzyme expression, elevated lipid peroxidation product content and oxidative system impairment was caused by the HFD as early as the first week of the experiment. Based on these results, we may postulate that changes in AA content may be an early indicator of inflammation and irreversible changes in NAFLD progression. View Full-Text
Keywords: arachidonic acid; inflammation; inflammatory precursor; lipid accumulation; oxidative stress; non-alcoholic fatty liver disease arachidonic acid; inflammation; inflammatory precursor; lipid accumulation; oxidative stress; non-alcoholic fatty liver disease
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MDPI and ACS Style

Sztolsztener, K.; Chabowski, A.; Harasim-Symbor, E.; Bielawiec, P.; Konstantynowicz-Nowicka, K. Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development. Biomolecules 2020, 10, 1133. https://doi.org/10.3390/biom10081133

AMA Style

Sztolsztener K, Chabowski A, Harasim-Symbor E, Bielawiec P, Konstantynowicz-Nowicka K. Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development. Biomolecules. 2020; 10(8):1133. https://doi.org/10.3390/biom10081133

Chicago/Turabian Style

Sztolsztener, Klaudia, Adrian Chabowski, Ewa Harasim-Symbor, Patrycja Bielawiec, and Karolina Konstantynowicz-Nowicka. 2020. "Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development" Biomolecules 10, no. 8: 1133. https://doi.org/10.3390/biom10081133

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