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Open AccessArticle

An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line

1
Department of Molecular Biotechnology and Genetics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland
2
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland
3
Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland
4
Faculté des Sciences et Technologies, La Rochelle Université, UMRi CNRS 7266 LIENSs, CEDEX 1, F-17042 La Rochelle, France
5
Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(4), 510; https://doi.org/10.3390/biom10040510
Received: 23 February 2020 / Revised: 23 March 2020 / Accepted: 25 March 2020 / Published: 27 March 2020
(This article belongs to the Special Issue Selected Papers from Bio.Natural Meeting 2019)
Many biologically-active plant-derived compounds have therapeutic or chemopreventive effects. The use of plant in vitro cultures in conjunction with modern genetic engineering techniques allows greater amounts of valuable secondary metabolites to be obtained without interfering with the natural environment. This work presents the first findings concerning the acquisition of transgenic hairy roots of Senna obtusifolia overexpressing the gene encoding squalene synthase 1 from Panax ginseng (PgSS1) (SOPSS hairy loot lines) involved in terpenoid biosynthesis. Our results confirm that one of PgSS1-overexpressing hairy root line extracts (SOPSS2) possess a high cytotoxic effect against a human acute lymphoblastic leukemia (NALM6) cell line. Further analysis of the cell cycle, the expression of apoptosis-related genes (TP53, PUMA, NOXA, BAX) and the observed decrease in mitochondrial membrane potential also confirmed that the SOPSS2 hairy root extract displays the highest effects; similar results were also obtained for this extract combined with doxorubicin. The high cytotoxic activity, observed both alone or in combination with doxorubicin, may be due to the higher content of betulinic acid as determined by HPLC analysis. Our results suggest synergistic effects of tested extract (betulinic acid in greater amount) with doxorubicin which may be used in the future to develop new effective strategies of cancer chemosensitization. View Full-Text
Keywords: Senna obtusifolia; transgenic hairy roots; betulinic acid; anticancer properties; NALM6 leukemia cell line Senna obtusifolia; transgenic hairy roots; betulinic acid; anticancer properties; NALM6 leukemia cell line
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MDPI and ACS Style

Kowalczyk, T.; Sitarek, P.; Toma, M.; Picot, L.; Wielanek, M.; Skała, E.; Śliwiński, T. An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line. Biomolecules 2020, 10, 510. https://doi.org/10.3390/biom10040510

AMA Style

Kowalczyk T, Sitarek P, Toma M, Picot L, Wielanek M, Skała E, Śliwiński T. An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line. Biomolecules. 2020; 10(4):510. https://doi.org/10.3390/biom10040510

Chicago/Turabian Style

Kowalczyk, Tomasz; Sitarek, Przemysław; Toma, Monika; Picot, Laurent; Wielanek, Marzena; Skała, Ewa; Śliwiński, Tomasz. 2020. "An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line" Biomolecules 10, no. 4: 510. https://doi.org/10.3390/biom10040510

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