Next Article in Journal
Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in Escherichia coli, Purification, and Characterization
Previous Article in Journal
A Synthetic CPP33-Conjugated HOXA9 Active Domain Peptide Inhibits Invasion Ability of Non-Small Lung Cancer Cells
Previous Article in Special Issue
Post-Myocardial Infarction Ventricular Remodeling Biomarkers—The Key Link between Pathophysiology and Clinic
Open AccessArticle

Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes

1
Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, Italy
2
Department of Medicine and Health Sciences, University of Molise, 86100 Campobasso, Italy
3
Department of Vascular Physiopathology, IRCCS Neuromed, 86077 Pozzilli, Italy
4
Department of Translational Medical Sciences, Federico II University of Naples, 80131 Naples, Italy
5
Istituti Clinici Scientifici Maugeri SPA-Società Benefit, IRCCS, 82037 Telese Terme (BN), Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2020, 10(11), 1590; https://doi.org/10.3390/biom10111590
Received: 11 October 2020 / Revised: 19 November 2020 / Accepted: 20 November 2020 / Published: 23 November 2020
(This article belongs to the Special Issue Molecular Biomarkers In Cardiology)
Heart Failure (HF) is a syndrome, which implies the existence of different phenotypes. The new categorization includes patients with preserved ejection fraction (HFpEF), mid-range EF (HFmrEF), and reduced EF (HFrEF) but the molecular mechanisms involved in these HF phenotypes have not yet been exhaustively investigated. Sirt1 plays a crucial role in biological processes strongly related to HF. This study aimed to evaluate whether Sirt1 activity was correlated with EF and other parameters in HFpEF, HFmrEF, and HFrEF. Seventy patients, HFpEF (n = 23), HFmrEF (n = 23) and HFrEF (n = 24), were enrolled at the Cardiology Unit of the University Hospital of Salerno. Sirt1 activity was measured in peripheral blood mononuclear cells (PBMCs). Angiotensin-Converting Enzyme 2 (ACE2) activity, Tumor Necrosis Factor-alpha (TNF-α) and Brain Natriuretic Peptide (BNP) levels were quantified in plasma. HFpEF showed lower Sirt1 and ACE2 activities than both HFmrEF and HFrEF (p < 0.0001), without difference compared to No HF controls. In HFmrEF and HFrEF a very strong correlation was found between Sirt1 activity and EF (r2 = 0.899 and r2 = 0.909, respectively), and between ACE2 activity and Sirt1 (r2 = 0.801 and r2 = 0.802, respectively). HFrEF showed the highest TNF-α levels without reaching statistical significance. Significant differences in BNP were found among the groups, with the highest levels in the HFrEF. Determining Sirt1 activity in PBMCs is useful to distinguish the HF patients’ phenotypes from each other, especially HFmrEF/HFrEF from HFpEF. View Full-Text
Keywords: heart failure with preserved ejection fraction; heart failure with mid-range ejection fraction; heart failure with reduced ejection fraction; sirtuins; Sirt1 heart failure with preserved ejection fraction; heart failure with mid-range ejection fraction; heart failure with reduced ejection fraction; sirtuins; Sirt1
Show Figures

Figure 1

MDPI and ACS Style

Conti, V.; Corbi, G.; Polito, M.V.; Ciccarelli, M.; Manzo, V.; Torsiello, M.; De Bellis, E.; D’Auria, F.; Vitulano, G.; Piscione, F.; Carrizzo, A.; Di Pietro, P.; Vecchione, C.; Ferrara, N.; Filippelli, A. Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes. Biomolecules 2020, 10, 1590.

AMA Style

Conti V, Corbi G, Polito MV, Ciccarelli M, Manzo V, Torsiello M, De Bellis E, D’Auria F, Vitulano G, Piscione F, Carrizzo A, Di Pietro P, Vecchione C, Ferrara N, Filippelli A. Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes. Biomolecules. 2020; 10(11):1590.

Chicago/Turabian Style

Conti, Valeria; Corbi, Graziamaria; Polito, Maria V.; Ciccarelli, Michele; Manzo, Valentina; Torsiello, Martina; De Bellis, Emanuela; D’Auria, Federica; Vitulano, Gennaro; Piscione, Federico; Carrizzo, Albino; Di Pietro, Paola; Vecchione, Carmine; Ferrara, Nicola; Filippelli, Amelia. 2020. "Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes" Biomolecules 10, no. 11: 1590.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop