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Polymorphic Protective Dps–DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering

1
National Research Center “Kurchatov Institute”, Akademika Kurchatova pl., 1, 123182 Moscow, Russia
2
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia
3
Moscow Institute of Physics and Technology, Institutsky lane 9, 141700 Dolgoprudny, Moscow Region, Russia
4
Shemyakin-Ovchinnikov Institute of bioorganic chemistry of Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
5
Frumkin Institute of Physical Chemistry and Electrochemistry of Russian Academy of Sciences, Leninsky prospect, 31, 119071 Moscow, Russia
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(1), 39; https://doi.org/10.3390/biom10010039
Received: 6 November 2019 / Revised: 19 December 2019 / Accepted: 22 December 2019 / Published: 26 December 2019
(This article belongs to the Section Biomacromolecules)
Rapid increase of intracellular synthesis of specific histone-like Dps protein that binds DNA to protect the genome against deleterious factors leads to in cellulo crystallization—one of the most curious processes in the area of life science at the moment. However, the actual structure of the Dps–DNA co-crystals remained uncertain in the details for more than two decades. Cryo-electron tomography and small-angle X-ray scattering revealed polymorphous modifications of the co-crystals depending on the buffer parameters. Two different types of the Dps–DNA co-crystals are formed in vitro: triclinic and cubic. Three-dimensional reconstruction revealed DNA and Dps molecules in cubic co-crystals, and the unit cell parameters of cubic lattice were determined consistently by both methods. View Full-Text
Keywords: biocrystallization; Dps; DNA; co-crystals; cryo-electron tomography; small-angle X-ray scattering biocrystallization; Dps; DNA; co-crystals; cryo-electron tomography; small-angle X-ray scattering
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Kamyshinsky, R.; Chesnokov, Y.; Dadinova, L.; Mozhaev, A.; Orlov, I.; Petoukhov, M.; Orekhov, A.; Shtykova, E.; Vasiliev, A. Polymorphic Protective Dps–DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering. Biomolecules 2020, 10, 39.

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