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Sci. Pharm.
Volume 84
Issue 1
10.3797/scipharm.ISP.2015.01
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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Article

Study of HMG-CoA Reductase Inhibition Activity of the Hydrolyzed Product of Snakehead Fish (Channa striata) Skin Collagen with 50 kDa Collagenase from Bacillus licheniformis F11.4

by
Agnes VIRGINIA
,
Heni RACHMAWATI
,
Catur RIANI
and
Debbie S. RETNONINGRUM
*
School of Pharmacy, Institut Teknologi Bandung, Ganeca 10, 40132 Bandung, Indonesia.
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2016, 84(1), 81-88; https://doi.org/10.3797/scipharm.ISP.2015.01
Submission received: 5 September 2015 / Accepted: 15 December 2015 / Published: 14 February 2016
Versions Notes

Abstract

Bioactive peptides produced from enzymatic hydrolysis fibrous protein have been proven to have several biological activities. Previous study showed that the hydrolysis product of snakehead fish skin collagen with 26 kDa collagenase from Bacillus licheniformis F11.4 showed HMG-CoA (HMGR) inhibition activity. The aim of this research was to determine the ability of the hydrolysis product produced from snakehead fish skin collagen hydrolysed by 50 kDa collagenase from B. licheniformis F11.4 in inhibiting HMGR activity. Snakehead fish skin collagen was extracted using an acid method and collagenase was produced from B. licheniformis F11.4 using half-strength Luria Bertani (LB) medium containing 5% collagen. Crude collagenase was concentrated and fractionated using the DEAE Sephadex A-25 column eluted with increasing gradient concentrations of NaCl. Collagen, collagenase, and fractions were analyzed using SDS-PAGE and collagenolytic activity was analyzed by the zymography method. Collagenase with 50 kDa molecular weight presented in fraction one was used to hydrolyze the collagen. The reaction was done in 18 hours at 50°C. The hydrolysis product using 3.51 μg collagen and 9 ng collagenase showed 25.8% inhibition activity against pravastatin. This work shows for the first time that the hydrolysis product of snakehead fish skin collagen and 50 kDa collagenase from B. licheniformis F11.4 has potential as an anticholesterol agent.
Keywords: Bacillus licheniformis F11.4; HMGR inhibitor; Snakehead fish skin collagen; Collagenase Bacillus licheniformis F11.4; HMGR inhibitor; Snakehead fish skin collagen; Collagenase

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MDPI and ACS Style

VIRGINIA, A.; RACHMAWATI, H.; RIANI, C.; RETNONINGRUM, D.S. Study of HMG-CoA Reductase Inhibition Activity of the Hydrolyzed Product of Snakehead Fish (Channa striata) Skin Collagen with 50 kDa Collagenase from Bacillus licheniformis F11.4. Sci. Pharm. 2016, 84, 81-88. https://doi.org/10.3797/scipharm.ISP.2015.01

AMA Style

VIRGINIA A, RACHMAWATI H, RIANI C, RETNONINGRUM DS. Study of HMG-CoA Reductase Inhibition Activity of the Hydrolyzed Product of Snakehead Fish (Channa striata) Skin Collagen with 50 kDa Collagenase from Bacillus licheniformis F11.4. Scientia Pharmaceutica. 2016; 84(1):81-88. https://doi.org/10.3797/scipharm.ISP.2015.01

Chicago/Turabian Style

VIRGINIA, Agnes, Heni RACHMAWATI, Catur RIANI, and Debbie S. RETNONINGRUM. 2016. "Study of HMG-CoA Reductase Inhibition Activity of the Hydrolyzed Product of Snakehead Fish (Channa striata) Skin Collagen with 50 kDa Collagenase from Bacillus licheniformis F11.4" Scientia Pharmaceutica 84, no. 1: 81-88. https://doi.org/10.3797/scipharm.ISP.2015.01

APA Style

VIRGINIA, A., RACHMAWATI, H., RIANI, C., & RETNONINGRUM, D. S. (2016). Study of HMG-CoA Reductase Inhibition Activity of the Hydrolyzed Product of Snakehead Fish (Channa striata) Skin Collagen with 50 kDa Collagenase from Bacillus licheniformis F11.4. Scientia Pharmaceutica, 84(1), 81-88. https://doi.org/10.3797/scipharm.ISP.2015.01

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