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Article

Pentavalent Disabled Infectious Single Animal (DISA)/DIVA Vaccine Provides Protection in Sheep and Cattle against Different Serotypes of Bluetongue Virus

1
Department of Virology, Wageningen Bioveterinary Research (WBVR), 8200 RA Lelystad, The Netherlands
2
Department of Biochemistry, Centre for Human Metabolomics, North-West University, Potchefstroom 2520, South Africa
3
Public Health Department, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Amine A. Kamen
Vaccines 2021, 9(10), 1150; https://doi.org/10.3390/vaccines9101150
Received: 30 August 2021 / Revised: 28 September 2021 / Accepted: 5 October 2021 / Published: 9 October 2021
(This article belongs to the Section Attenuated/Inactivated/Live and Vectored Vaccines)
Bluetongue (BT) is a midge-borne OIE-notifiable disease of ruminants caused by the bluetongue virus (BTV). There are at least 29 BTV serotypes as determined by serum neutralization tests and genetic analyses of genome segment 2 encoding serotype immunodominant VP2 protein. Large parts of the world are endemic for multiple serotypes. The most effective control measure of BT is vaccination. Conventionally live-attenuated and inactivated BT vaccines are available but have their specific pros and cons and are not DIVA compatible. The prototype Disabled Infectious Single Animal (DISA)/DIVA vaccine based on knockout of NS3/NS3a protein of live-attenuated BTV, shortly named DISA8, fulfills all criteria for modern veterinary vaccines of sheep. Recently, DISA8 with an internal in-frame deletion of 72 amino acid codons in NS3/NS3a showed a similar ideal vaccine profile in cattle. Here, the DISA/DIVA vaccine platform was applied for other serotypes, and pentavalent DISA/DIVA vaccine for “European” serotypes 1, 2, 3, 4, 8 was studied in sheep and cattle. Protection was demonstrated for two serotypes, and neutralization Ab titers indicate protection against other included serotypes. The DISA/DIVA vaccine platform is flexible in use and generates monovalent and multivalent DISA vaccines to combat specific field situations with respect to Bluetongue. View Full-Text
Keywords: bluetongue virus; vaccine platform; DISA; DIVA; NS3; serotype exchange bluetongue virus; vaccine platform; DISA; DIVA; NS3; serotype exchange
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MDPI and ACS Style

van Rijn, P.A.; Maris-Veldhuis, M.A.; Spedicato, M.; Savini, G.; van Gennip, R.G.P. Pentavalent Disabled Infectious Single Animal (DISA)/DIVA Vaccine Provides Protection in Sheep and Cattle against Different Serotypes of Bluetongue Virus. Vaccines 2021, 9, 1150. https://doi.org/10.3390/vaccines9101150

AMA Style

van Rijn PA, Maris-Veldhuis MA, Spedicato M, Savini G, van Gennip RGP. Pentavalent Disabled Infectious Single Animal (DISA)/DIVA Vaccine Provides Protection in Sheep and Cattle against Different Serotypes of Bluetongue Virus. Vaccines. 2021; 9(10):1150. https://doi.org/10.3390/vaccines9101150

Chicago/Turabian Style

van Rijn, Piet A., Mieke A. Maris-Veldhuis, Massimo Spedicato, Giovanni Savini, and René G. P. van Gennip. 2021. "Pentavalent Disabled Infectious Single Animal (DISA)/DIVA Vaccine Provides Protection in Sheep and Cattle against Different Serotypes of Bluetongue Virus" Vaccines 9, no. 10: 1150. https://doi.org/10.3390/vaccines9101150

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