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Open AccessArticle

A Simple and a Reliable Method to Quantify Antioxidant Activity In Vivo

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Department of Pharmaceutical Technology and Chemistry, School of Pharmacy and Nutrition, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain
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Department of Environmental Biology, School of Sciences, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain
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IDISNA-Instituto de Investigación Biosanitaria de Navarra, 31008 Pamplona, Spain
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Department of Biochemistry & Genetics, School of Sciences, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(5), 142; https://doi.org/10.3390/antiox8050142
Received: 3 April 2019 / Revised: 16 May 2019 / Accepted: 20 May 2019 / Published: 22 May 2019
The characterization of compounds with antioxidant activity is of great interest due to their ability to reduce reactive oxygen species production and, therefore, prevent some age-related diseases. Its antioxidant capacity can be analyzed by different methods both in vitro and in vivo. Caenorhabditis elegans is an in vivo model widely used in ageing research. Until now, available tests analyze functional effects in the worms, so the antioxidant activity of the compound is indirectly monitored. We have developed a simple and a reliable method to quantify internal antioxidant activity in vivo. To validate this method, we analyzed an aqueous green tea extract and two other compounds with a well-known antioxidant activity and without this activity. The results obtained (EC50 green tea = 21.76 ± 1.28 µg/mL; EC50 positive control = 8.50 ± 0.33 µg/mL; negative control EC50 > 500 µg/mL) can help in the design of further in vivo experiments. Thus, our method can be used as a previous screening capable of reducing the gap between in vitro and in vivo assays. View Full-Text
Keywords: C. elegans; DPPH scavenging; green tea; rosmarinic acid C. elegans; DPPH scavenging; green tea; rosmarinic acid
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MDPI and ACS Style

de Torre, M.P.; Cavero, R.Y.; Calvo, M.I.; Vizmanos, J.L. A Simple and a Reliable Method to Quantify Antioxidant Activity In Vivo. Antioxidants 2019, 8, 142.

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