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Volume 11
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10.3390/antiox11091640
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Article

Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

by
Jing-Tao Sun
1,
Jin-Dong Yuan
1,
Qi Zhang
1,
Xin Luo
1,
Xin-Yue Qi
1,
Jia-Hui Liu
1,
Xi-Qing Jiang
1,
Sanghoon Lee
2,
Anukul Taweechaipaisankul
3,
Zhong-Hua Liu
1,* and
Jun-Xue Jin
1,*
1
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin 150030, China
2
Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea
3
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand
*
Authors to whom correspondence should be addressed.
Antioxidants 2022, 11(9), 1640; https://doi.org/10.3390/antiox11091640
Submission received: 23 June 2022 / Revised: 20 August 2022 / Accepted: 22 August 2022 / Published: 24 August 2022
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Abstract

This study aimed to determine the underlying mechanism of ramelteon on the competence of oocyte and subsequent embryo development in pigs during in vitro maturation (IVM). Our results showed that the cumulus expansion index was significantly lower in the control group compared to the ramelteon groups (p < 0.05). Moreover, supplementation of 10−11 and 10−9 M ramelteon significantly increased the cumulus expansion and development-related genes expression, and reduced apoptosis in cumulus cells (p < 0.05). In oocytes, the nuclear maturation rate was significantly improved in 10−11, 10−9, and 10−7 M ramelteon groups compared to the control (p < 0.05). Additionally, the level of intracellular GSH was significantly increased and ROS was significantly decreased in ramelteon-supplemented groups, and the gene expression of oocyte development and apoptosis were significantly up- and down-regulated by 10−11 and 10−9 M ramelteon (p < 0.05), respectively. The immunofluorescence results showed that the protein levels of GDF9, BMP15, SOD1, CDK1, and PGC1α were significantly increased by 10−11 M ramelteon compared to the control (p < 0.05). Although there was no significant difference in cleavage rate, the blastocyst formation rate, total cell numbers, and hatching/-ed rate were significantly improved in 10−11 M ramelteon group compared to the control (p < 0.05). Furthermore, embryo development, hatching, and mitochondrial biogenesis-related genes were dramatically up-regulated by 10−11 M ramelteon (p < 0.05). In addition, the activities of lipogenesis and lipolysis in oocytes were dramatically increased by 10−11 M ramelteon compared to the control (p < 0.05). In conclusion, supplementation of 10−11 M ramelteon during IVM improved the oocyte maturation and subsequent embryo development by reducing oxidative stress and maintenance of lipid homeostasis.
Keywords: ramelteon; oocyte maturation; oxidative stress; lipid homeostasis; pig ramelteon; oocyte maturation; oxidative stress; lipid homeostasis; pig
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MDPI and ACS Style

Sun, J.-T.; Yuan, J.-D.; Zhang, Q.; Luo, X.; Qi, X.-Y.; Liu, J.-H.; Jiang, X.-Q.; Lee, S.; Taweechaipaisankul, A.; Liu, Z.-H.; et al. Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes. Antioxidants 2022, 11, 1640. https://doi.org/10.3390/antiox11091640

AMA Style

Sun J-T, Yuan J-D, Zhang Q, Luo X, Qi X-Y, Liu J-H, Jiang X-Q, Lee S, Taweechaipaisankul A, Liu Z-H, et al. Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes. Antioxidants. 2022; 11(9):1640. https://doi.org/10.3390/antiox11091640

Chicago/Turabian Style

Sun, Jing-Tao, Jin-Dong Yuan, Qi Zhang, Xin Luo, Xin-Yue Qi, Jia-Hui Liu, Xi-Qing Jiang, Sanghoon Lee, Anukul Taweechaipaisankul, Zhong-Hua Liu, and et al. 2022. "Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes" Antioxidants 11, no. 9: 1640. https://doi.org/10.3390/antiox11091640

APA Style

Sun, J.-T., Yuan, J.-D., Zhang, Q., Luo, X., Qi, X.-Y., Liu, J.-H., Jiang, X.-Q., Lee, S., Taweechaipaisankul, A., Liu, Z.-H., & Jin, J.-X. (2022). Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes. Antioxidants, 11(9), 1640. https://doi.org/10.3390/antiox11091640

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