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Article

Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar

1
Faculty of Veterinary Science, University of Thessaly, 431 00 Karditsa, Greece
2
Animal and Plant Health Agency, Addlestone KT15 3NB, UK
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Faculty of Public and Integrated Health, University of Thessaly, 431 00 Karditsa, Greece
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SaBio, Instituto de Investigación en Recursos Cinegéticos IREC, 13005 Ciudad Real, Spain
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SABIOTEC, Camino de Moledores s/n, 13005 Ciudad Real, Spain
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Faculty of Forestry, Wood Science and Design, 431 00 Karditsa, Greece
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Faculty of Animal Science, University of Thessaly, 412 22 Larissa, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: Hirokazu Kimura
Microorganisms 2021, 9(5), 904; https://doi.org/10.3390/microorganisms9050904
Received: 27 March 2021 / Revised: 16 April 2021 / Accepted: 20 April 2021 / Published: 23 April 2021
(This article belongs to the Special Issue Farm Animal and Wildlife Zoonotic Microorganisms)
The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative), and Trichinella (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell B. suis 1330 antigen, and an E/S T. spiralis antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for M. bovis, 0.98 and 0.86; for B. suis, 1.00 and 0.97; and for T. spiralis, 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar. View Full-Text
Keywords: multiplex bead assay; Mycobacterium bovis; Brucella suis; Trichinella spiralis; wild boar; serological assay multiplex bead assay; Mycobacterium bovis; Brucella suis; Trichinella spiralis; wild boar; serological assay
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MDPI and ACS Style

Touloudi, A.; Valiakos, G.; Cawthraw, S.; Kostoulas, P.; Gortázar, C.; Boadella, M.; Giannakopoulos, A.; Birtsas, P.; Sofia, M.; Athanasiou, L.V.; Satra, M.; Athanasakopoulou, Z.; Kantere, M.; Spyrou, V.; Petrovska, L.; Billinis, C. Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar. Microorganisms 2021, 9, 904. https://doi.org/10.3390/microorganisms9050904

AMA Style

Touloudi A, Valiakos G, Cawthraw S, Kostoulas P, Gortázar C, Boadella M, Giannakopoulos A, Birtsas P, Sofia M, Athanasiou LV, Satra M, Athanasakopoulou Z, Kantere M, Spyrou V, Petrovska L, Billinis C. Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar. Microorganisms. 2021; 9(5):904. https://doi.org/10.3390/microorganisms9050904

Chicago/Turabian Style

Touloudi, Antonia, George Valiakos, Shaun Cawthraw, Polychronis Kostoulas, Christian Gortázar, Mariana Boadella, Alexios Giannakopoulos, Periklis Birtsas, Marina Sofia, Labrini V. Athanasiou, Maria Satra, Zoi Athanasakopoulou, Maria Kantere, Vassiliki Spyrou, Liljana Petrovska, and Charalambos Billinis. 2021. "Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar" Microorganisms 9, no. 5: 904. https://doi.org/10.3390/microorganisms9050904

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