Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene

Peng, Zhong; Hu, Zizhe; Li, Zugang; Li, Xiaosong; Jia, Chaoying; Zhang, Xiaoxue; Wu, Bin; Chen, Huanchun; Wang, Xiangru

doi:10.3390/microorganisms7110558

Open AccessArticle

Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene

by

Zhong Peng

^1,2,†

,

Zizhe Hu

^1,2,†,

Zugang Li

^1,2,

Xiaosong Li

^1,2,

Chaoying Jia

^1,2,

Xiaoxue Zhang

^1,2,

Bin Wu

^1,2

,

Huanchun Chen

^1,2 and

Xiangru Wang

^1,2,*

¹

State Key Laboratory of Agricultural Microbiology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China

²

The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Microorganisms 2019, 7(11), 558; https://doi.org/10.3390/microorganisms7110558

Submission received: 23 October 2019 / Revised: 8 November 2019 / Accepted: 9 November 2019 / Published: 12 November 2019

(This article belongs to the Special Issue Antimicrobial Resistance in Livestock)

Download

Browse Figures

Versions Notes

Abstract

Enterobacteriaceae having chromosomally-encoded mcr-1 is rarely reported. In this study, we recovered a chromosomal mcr-1 carrying Escherichia coli, designated HeN100, from the feces of a diarrheal pig in China. Antimicrobial susceptibility testing showed that HeN100 was resistant to three aminoglycosides, twelve β-lactams including three carbapenems, one phenicol, two tetracyclines, two fluoroquinolones, nitrofurantoin, and colistin tested. Oxford Nanopore MinION sequencing revealed that the complete genomes of the multidrug resistant (MDR) HeN100 consisted of a single circular chromosome and five circular plasmids. Bioinformatical analysis determined HeN100 as ST695 and it contained many acquired resistance genes responsible for its MDR phenotypes, including colistin resistance mcr-1 and the carbapenem resistance bla_NDM-1, and most of these genes were located on plasmids. However, the mcr-1 was found on the chromosome, and it was located between an IS30-like element ISApl1 and a PAP2-like encoding gene. These three genes consisted of an “ISApl1-mcr-1-orf” segment and inserted in high AT-rich regions. Finally, we found the bla_NDM-1 was carried on an IncFII type conjugative plasmid. The conjugation frequency of this plasmid was 7.61 ± 2.11 × 10⁻⁵ per recipient, and its conjugation conferred resistance to carbapenems and other β-lactams, as well as aminoglycosides. The spread of this mcr-1/bla_NDM-1-carrying E. coli ST695 represents a great concern of public health.

Keywords: Escherichia coli; ST695; antibiotic resistance; Oxford Nanopore MinION sequencing; chromosomally-encoded mcr-1; plasmid-carrying bla_NDM-1; plasmid conjugation

Graphical Abstract

Share and Cite

MDPI and ACS Style

Peng, Z.; Hu, Z.; Li, Z.; Li, X.; Jia, C.; Zhang, X.; Wu, B.; Chen, H.; Wang, X. Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene. Microorganisms 2019, 7, 558. https://doi.org/10.3390/microorganisms7110558

AMA Style

Peng Z, Hu Z, Li Z, Li X, Jia C, Zhang X, Wu B, Chen H, Wang X. Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene. Microorganisms. 2019; 7(11):558. https://doi.org/10.3390/microorganisms7110558

Chicago/Turabian Style

Peng, Zhong, Zizhe Hu, Zugang Li, Xiaosong Li, Chaoying Jia, Xiaoxue Zhang, Bin Wu, Huanchun Chen, and Xiangru Wang. 2019. "Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene" Microorganisms 7, no. 11: 558. https://doi.org/10.3390/microorganisms7110558

APA Style

Peng, Z., Hu, Z., Li, Z., Li, X., Jia, C., Zhang, X., Wu, B., Chen, H., & Wang, X. (2019). Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene. Microorganisms, 7(11), 558. https://doi.org/10.3390/microorganisms7110558

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI