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Pathogens 2018, 7(2), 41; https://doi.org/10.3390/pathogens7020041

Association and Expression of Virulence from Plasmids of the Group B Strain in Pseudomonas syringae pv. eriobotryae

1
Agricultural Genetics Institute, Pham Van Dong Street, Hanoi 122300, Vietnam
2
Division of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Higashi Hiroshima 739-8529, Japan
*
Author to whom correspondence should be addressed.
Received: 5 February 2018 / Revised: 10 April 2018 / Accepted: 12 April 2018 / Published: 14 April 2018
(This article belongs to the Section Plant Pathogens)
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Abstract

Pseudomonas syringae pv. eriobotryae causes serious stem canker in loquat (Eriobotrya japonica) trees. This study was conducted to determine whether plasmids are involved with its virulence. The strain NAE89, which belonged to the B group, harbored two plasmids at approximately 6.2 and 50 Mdal that caused stem canker and halo leaf spots on loquat plants. Following digestion with BamHI and ligation into the BamHI cloning site of the broad range host cosmid pLAFR3, four DNA fragments at 3.8, 6.6, 12.3, and 22.8 kb were generated. Although the plasmid-encoded virulence gene psvA was undigested with the BamHI, the halo leaf spot gene may be adjacent to the psvA gene was digested. A pLAFR3 cosmid clone was introduced into the non-pathogenic PE0 and NAE89-1 strains by triparental matings and the pathogenicity was recovered. As a result, the pLAFR3 cosmid clone was introduced into the largest size DNA fragment of 22.8 kb and determined to be the causal agent of canker on the stem of the loquat. This study revealed that the psvA gene, previously found in the 50 Mdal plasmid, was also observed in the 22.8 kb DNA fragment. View Full-Text
Keywords: Pseudomonas syringae pv. eriobotryae; pigment; pathogenic; psvA; virulence gene Pseudomonas syringae pv. eriobotryae; pigment; pathogenic; psvA; virulence gene
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Khanh, T.D.; Xuan, T.D. Association and Expression of Virulence from Plasmids of the Group B Strain in Pseudomonas syringae pv. eriobotryae. Pathogens 2018, 7, 41.

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