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Open AccessArticle

Analysis of the Metaphase Chromosome Karyotypes in Imaginal Discs of Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus (Diptera: Culicidae) Mosquitoes

1
Laboratory of Evolution Cytogenetics, Tomsk State University, Lenin st, 36, Tomsk 634050, Russia
2
Laboratory of Evolutionary Genomics of Insects, the Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev ave., 10, Novosibirsk 630090, Russia
*
Author to whom correspondence should be addressed.
Insects 2020, 11(1), 63; https://doi.org/10.3390/insects11010063
Received: 11 December 2019 / Revised: 15 January 2020 / Accepted: 16 January 2020 / Published: 19 January 2020
(This article belongs to the Special Issue Genomics and Cytogenetics of Mosquitoes)
In this study, cytogenetic analysis of the metaphase chromosomes from imaginal discs of Aedes (Diptera: Culicidae) mosquitoes—Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus—was performed. The patterns of C-banding and DAPI staining of the heteroсhromatin and the length of the chromosomes demonstrate species specificity. In particular, the Ae. punctor chromosomes are the shortest compared with Ae. communis, Ae. intrudens, and Ae. rossicus, and they also carry additional C and DAPI bands in intercalary regions. The Ae. intrudens chromosomes are the longest, they have pericentromeric C bands, and they almost lack any DAPI bands near the centromere of chromosome 3 versus Ae. communis, which has the largest pericentromeric DAPI blocks in all three chromosome pairs. Ae. rossicus also possesses DAPI bands in the centromeric regions of all chromosomes, but their staining is weaker compared with those of Ae. communis. Therefore, the analysis of karyotypes is a tool for species-level identification of these mosquitoes. View Full-Text
Keywords: mosquitoes; Culicidae; Aedes; C-banding; DAPI; mitotic chromosomes; imaginal discs mosquitoes; Culicidae; Aedes; C-banding; DAPI; mitotic chromosomes; imaginal discs
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Alekseeva, S.S.; Andreeva, Y.V.; Wasserlauf, I.E.; Sibataev, A.K.; Stegniy, V.N. Analysis of the Metaphase Chromosome Karyotypes in Imaginal Discs of Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus (Diptera: Culicidae) Mosquitoes. Insects 2020, 11, 63.

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