Next Article in Journal
Pan-Cancer Analysis of the Genomic Alterations and Mutations of the Matrisome
Previous Article in Journal
Early Change in the Plasma Levels of Circulating Soluble Immune Checkpoint Proteins in Patients with Unresectable Hepatocellular Carcinoma Treated by Lenvatinib or Transcatheter Arterial Chemoembolization
Article

TCGA mRNA Expression Analysis of the Heme Biosynthesis Pathway in Diffusely Infiltrating Gliomas: A Comparison of Typically 5-ALA Fluorescent and Non-Fluorescent Gliomas

1
Department of Neurosurgery, Medical University Vienna, 1090 Vienna, Austria
2
Comprehensive Cancer Center-Central Nervous System Tumours Unit, Medical University Vienna, 1090 Vienna, Austria
3
Department of Neurology, Division of Neuropathology and Neurochemistry, Medical University Vienna, 1090 Vienna, Austria
4
Department of Statistics, Ludwig-Maximilians-University, 80539 Munich, Germany
5
Department of Neurological Surgery, University of California, San Francisco, CA 94143, USA
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(8), 2043; https://doi.org/10.3390/cancers12082043
Received: 1 July 2020 / Revised: 20 July 2020 / Accepted: 22 July 2020 / Published: 24 July 2020
(This article belongs to the Section Cancer Therapy)
5-Aminolevulinic acid (5-ALA) is a fluorescent dye that after metabolization to Protoporphyrin IX (PpIX) by the heme biosynthesis pathway typically leads to visible fluorescence in WHO grade IV but not grade II gliomas. The exact mechanism for high PpIX levels in WHO grade IV gliomas and low PpIX levels in WHO grade II gliomas is not fully clarified. To detect relevant changes in mRNA expression, we performed an in-silico analysis of WHO grade II and IV glioma sequencing datasets provided by The Cancer Genome Atlas (TCGA) to investigate mRNA expression levels of relevant heme biosynthesis genes: Solute Carrier Family 15 Member 1 and 2 (SLC15A1 and SLC15A2), Aminolevulinate-Dehydratase (ALAD), Hydroxymethylbilane-Synthase (HMBS), Uroporphyrinogen-III-Synthase (UROS), Uroporphyrinogen-Decarboxylase (UROD), Coproporphyrinogen-Oxidase (CPOX), Protoporphyrinogen-Oxidase (PPOX), ATP-binding Cassette Subfamily B Member 6 (ABCB6)/G Member 2 (ABCG2) and Ferrochelatase (FECH). Altogether, 258 WHO grade II and 166 WHO grade IV samples were investigated. The mRNA expression levels showed significant differences in 8 of 11 examined genes between WHO grade II and IV gliomas. Significant differences in mRNA expression included increases of HMBS, UROD, FECH and PPOX as well as decreases of SLC15A2, ALAD, UROS and ABCB6 in WHO IV gliomas. Since the majority of changes was found in directions that might actually impair PpIX accumulation in WHO grade IV gliomas, additional studies are needed to analyze the corresponding factors of the heme biosynthesis also on protein level. View Full-Text
Keywords: 5-ALA; fluorescence; glioma; heme biosynthesis; TCGA; gene expression 5-ALA; fluorescence; glioma; heme biosynthesis; TCGA; gene expression
Show Figures

Figure 1

MDPI and ACS Style

Mischkulnig, M.; Kiesel, B.; Lötsch, D.; Roetzer, T.; Borkovec, M.; Wadiura, L.I.; Mercea, P.A.; Jaklin, F.J.; Hervey-Jumper, S.; Roessler, K.; Berger, M.S.; Widhalm, G.; Erhart, F. TCGA mRNA Expression Analysis of the Heme Biosynthesis Pathway in Diffusely Infiltrating Gliomas: A Comparison of Typically 5-ALA Fluorescent and Non-Fluorescent Gliomas. Cancers 2020, 12, 2043. https://doi.org/10.3390/cancers12082043

AMA Style

Mischkulnig M, Kiesel B, Lötsch D, Roetzer T, Borkovec M, Wadiura LI, Mercea PA, Jaklin FJ, Hervey-Jumper S, Roessler K, Berger MS, Widhalm G, Erhart F. TCGA mRNA Expression Analysis of the Heme Biosynthesis Pathway in Diffusely Infiltrating Gliomas: A Comparison of Typically 5-ALA Fluorescent and Non-Fluorescent Gliomas. Cancers. 2020; 12(8):2043. https://doi.org/10.3390/cancers12082043

Chicago/Turabian Style

Mischkulnig, Mario, Barbara Kiesel, Daniela Lötsch, Thomas Roetzer, Martin Borkovec, Lisa I. Wadiura, Petra A. Mercea, Florian J. Jaklin, Shawn Hervey-Jumper, Karl Roessler, Mitchel S. Berger, Georg Widhalm, and Friedrich Erhart. 2020. "TCGA mRNA Expression Analysis of the Heme Biosynthesis Pathway in Diffusely Infiltrating Gliomas: A Comparison of Typically 5-ALA Fluorescent and Non-Fluorescent Gliomas" Cancers 12, no. 8: 2043. https://doi.org/10.3390/cancers12082043

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop